miRNA Search Results

sort by 1 publications mentioning mml-mir-184 Open access articles that are associated with the species Macaca mulatta and mention the gene name mir-184. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.
1	[+] score: 40 Other miRNAs from this paper: hsa-mir-143, hsa-mir-154, hsa-mir-184, hsa-mir-34c, hsa-mir-299, hsa-mir-375, hsa-mir-383, hsa-mir-487a, ptr-mir-143, ptr-mir-154, ptr-mir-184, hsa-mir-499a, mml-mir-34c, mml-mir-143, mml-mir-154, mml-mir-299, mml-mir-375, mml-mir-383, mml-mir-487a, mml-mir-499, ptr-mir-34c, ptr-mir-375, ptr-mir-383, ptr-mir-487a, ptr-mir-499, ptr-mir-299, hsa-mir-499b To assess whether miRNA with expression divergence on the human lineage might be associated with human cognitive functions, we investigated the expression of genes targeted by five miRNA showing human-specific expression, according to multiple methodologies: miR-184, miR-487a, miR-383, miR-34c-5p and miR-299-3p (Figure 2). [score:7] Potential influence of miRNA divergence on neuronal functions was further confirmed by preferential expression of the corresponding miR-299-3p and miR-184 in cortical neurons, as well as verification of the predicted miRNA-target relationship in two human neuroblastoma cell lines. [score:5] This effect was significant for combined targets of the five miRNA, as well as for targets of miR-184 and miR-383 analyzed individually. [score:5] Furthermore, miR-184 targets were significantly enriched in “long-term potentiation” pathway – one of the few pathways directly connected to learning and memory formation [34], [35]. [score:4] Similarly, based on the DIANA-mirPath algorithm [33], targets of miR-184, miR-487a and miR-299-3p were significantly enriched in KEGG pathways that are related to neural functions (Table S10). [score:3] To further test association of miR-184 and miR-299-3p with neuronal functions, we determined their expression patterns in the human and macaque prefrontal cortex by in situ hybridization with specific LNA-probes (Table S11). [score:3] Based on the DIANA-mirPath algorithm, targets of miR-184, miR-487a and miR-299-3p were significantly enriched in KEGG pathways that are related to neural functions (Table S10). [score:3] Requiring significant support by at least two out of three methodologies (sequencing, microarrays and Q-PCR), expression changes in five miRNA (miR-184, miR-299-3p, miR-487a, miR-383 and miR-34c-5p) could be assigned to the human evolutionary lineage and two (miR-375 and miR-154*) to the chimpanzee evolutionary lineage (Figure 2). [score:3] Recent studies have also shown that miR-184 is involved in regulation of neural stem cell proliferation and differentiation [36]. [score:2] We chose three types of miRNA differences: (1) consistent by both methodologies: miR-383 and miR-34c-5p; (2) significant according to sequencing, but unconfirmed in the microarray experiment: miR-143 and miR-499; (3) significant according to sequencing, but not detected or masked on the microarrays: miR-184 and miR-299-3p. [score:1] 1002327.g004 Figure 4 In situ staining of miR-184 and miR-299-3p in prefrontal cortex. [score:1] In situ staining of miR-184 and miR-299-3p in prefrontal cortex. [score:1] We designed two LNA-probes complementary to miR-184 and miR-299-3p respectively (Table S11). [score:1] On the DNA sequence level, these miRNA tend to be conserved: miR-184 mature miRNA sequence is evolutionarily conserved from insects to humans, with only one nucleotide different at 3′end of mature sequence, while miR-383 and miR-34c-5p are classified as broadly conserved and miR-299-3p - as conserved among animal species [25], [31]. [score:1] [1 to 20 of 14 sentences]

To assess whether miRNA with expression divergence on the human lineage might be associated with human cognitive functions, we investigated the expression of genes targeted by five miRNA showing human-specific expression, according to multiple methodologies: miR-184, miR-487a, miR-383, miR-34c-5p and miR-299-3p (Figure 2). [score:7]

Potential influence of miRNA divergence on neuronal functions was further confirmed by preferential expression of the corresponding miR-299-3p and miR-184 in cortical neurons, as well as verification of the predicted miRNA-target relationship in two human neuroblastoma cell lines. [score:5]

This effect was significant for combined targets of the five miRNA, as well as for targets of miR-184 and miR-383 analyzed individually. [score:5]

Furthermore, miR-184 targets were significantly enriched in “long-term potentiation” pathway – one of the few pathways directly connected to learning and memory formation [34], [35]. [score:4]

Similarly, based on the DIANA-mirPath algorithm [33], targets of miR-184, miR-487a and miR-299-3p were significantly enriched in KEGG pathways that are related to neural functions (Table S10). [score:3]

To further test association of miR-184 and miR-299-3p with neuronal functions, we determined their expression patterns in the human and macaque prefrontal cortex by in situ hybridization with specific LNA-probes (Table S11). [score:3]

Based on the DIANA-mirPath algorithm, targets of miR-184, miR-487a and miR-299-3p were significantly enriched in KEGG pathways that are related to neural functions (Table S10). [score:3]

Requiring significant support by at least two out of three methodologies (sequencing, microarrays and Q-PCR), expression changes in five miRNA (miR-184, miR-299-3p, miR-487a, miR-383 and miR-34c-5p) could be assigned to the human evolutionary lineage and two (miR-375 and miR-154*) to the chimpanzee evolutionary lineage (Figure 2). [score:3]

Recent studies have also shown that miR-184 is involved in regulation of neural stem cell proliferation and differentiation [36]. [score:2]

We chose three types of miRNA differences: (1) consistent by both methodologies: miR-383 and miR-34c-5p; (2) significant according to sequencing, but unconfirmed in the microarray experiment: miR-143 and miR-499; (3) significant according to sequencing, but not detected or masked on the microarrays: miR-184 and miR-299-3p. [score:1]

1002327.g004 Figure 4 In situ staining of miR-184 and miR-299-3p in prefrontal cortex. [score:1]

In situ staining of miR-184 and miR-299-3p in prefrontal cortex. [score:1]

We designed two LNA-probes complementary to miR-184 and miR-299-3p respectively (Table S11). [score:1]

On the DNA sequence level, these miRNA tend to be conserved: miR-184 mature miRNA sequence is evolutionarily conserved from insects to humans, with only one nucleotide different at 3′end of mature sequence, while miR-383 and miR-34c-5p are classified as broadly conserved and miR-299-3p - as conserved among animal species [25], [31]. [score:1]

[1 to 20 of 14 sentences]

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1 publications mentioning mml-mir-184