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5 publications mentioning ptr-let-7a-1

Open access articles that are associated with the species Pan troglodytes and mention the gene name let-7a-1. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 27
The most downregulated miRNA during EB formation was miR-let-7a, which is of interest because let-7 has been shown to regulate developmental timing in Caenorhabditis elegans [22]. [score:6]
RISC -mediated target RNA cleavage activity was determined by in vitro cleavage of a [32]P-target mRNA that perfectly matched the miR-302b or let-7 sequence. [score:5]
RISC activity of miR-302b, a representative miRNA expressed in hES cells and not in HeLa cells, and let-7a was analyzed by incubating cell extracts with a [32]P-cap-labeled substrate mRNA that was perfectly complementary to miR-302b or let-7a. [score:3]
0002820.g004 Figure 4(A) Northern blot analysis of miR-302b and miR let-7a expression in hES and HeLa cells, respectively. [score:3]
Fig 4A demonstrates the specific expression of miR-302b and let-7 in hES and HeLa cells, respectively. [score:3]
Conversely, let-7 RISC in HeLa extracts cleaved let-7 target RNA while hES RISC did not show detectable activity in this experiment. [score:3]
Conservation of miRNAs was analyzed as described in Fig 1. (A) Northern blot analysis of miR-302b and miR let-7a expression in hES and HeLa cells, respectively. [score:3]
Since let-7a is abundant in HeLa, we selected let-7 as a control in our experiments. [score:1]
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2
[+] score: 17
Median miR-24 and Let-7a copy numbers did not vary significantly between tumor and non-tumor tissue samples from HBV- and HCV-infected subjects (one-way ANOVA), suggesting that the expression of these miRNAs is relatively constant in liver and that either could serve as a standard for normalizing miR-122 abundance. [score:3]
Notably, we observed no difference in the abundance of Let-7a in HBV -associated cancer and non-tumor tissues (p=0.52 by two-sided, paired t test), despite a prior report suggesting that Let-7a expression is regulated by the HBx protein and increased in abundance in HBV -associated HCC [32]. [score:3]
A strong positive correlation was evident between the abundance of miR-24 and Let-7a (Figure 3E, Spearman r [s]=0.7959, p<0.001 by two-tailed t test), suggesting that these miRNAs might belong to a common regulatory network and that either could be used to normalize miR-122 abundance. [score:2]
Shown in the panels at the top are the relative abundance of (A) U6 snRNA, (B) Let-7a, and (C) miR-24 miRNAs in paired tumor and non-tumor tissues from subjects with HBV or HCV infection, normalized to total RNA. [score:1]
There was no correlation between the RIN score and miR-24 or Let-7a abundance, suggesting that U6 snRNA may be less stable and more prone to degradation than the miRNAs. [score:1]
These data suggest that U6 is less stable than the miRNAs and provide additional support for the use of miR-24 (or Let-7a) as a standard against which to normalize miR-122 abundance (see Discussion). [score:1]
Interestingly, while the quantity of U6 snRNA detected correlated positively with the RIN score (Spearman r [s] = 0.5216, two-tailed p = 0.0001) (Figure S1A in), this was not the case with miR-24 or Let-7a (r [s] = -0.124 and -0.045, respectively). [score:1]
Quantification of miR-122, miR-191, Let-7a, miR-24, and the small nuclear RNA (snRNA) U6 was carried out by quantitative reverse-transcription, polymerase chain reaction (qRT-PCR) in a two-step process. [score:1]
In the lower set of panels, (D) U6, (E) Let-7a, and (F) miR-122 abundance are plotted as a function of miR-24 abundance. [score:1]
Consistent with this, Let-7a was found to have greater biological stability and to be superior to U6 for normalization of miRNA abundance in previous studies of rat hepatocyte RNA [48]. [score:1]
0076867.g003 Figure 3Shown in the panels at the top are the relative abundance of (A) U6 snRNA, (B) Let-7a, and (C) miR-24 miRNAs in paired tumor and non-tumor tissues from subjects with HBV or HCV infection, normalized to total RNA. [score:1]
Similar associations were found when miR-122 abundance was normalized to Let-7a (data not shown). [score:1]
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3
[+] score: 3
rs17123507 was the only one located in the exon region (3′UTR) of FLJ33706 among a tandem set of putative binding sites of let-7, a brain-expressed miRNA implicated in neuron specification [31]. [score:3]
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4
[+] score: 2
This oligomer effectively prevented let-7 action in vitro, in cultured HeLa cells following transfection using a liposomal reagent, and in C. elegans after injection. [score:1]
The first application of this approach used an RNA oligomer complementary to the miRNA let-7, with 2′-O-methyl (2′-OMe) modifications at each position [21]. [score:1]
[1 to 20 of 2 sentences]
5
[+] score: 1
The comparisons among fish (Figure 5B) revealed that conservation between pufferfishes and zebrafish sequences was largely limited to coding regions and to a microRNA (family let-7) positioned upstream of the HADH2 gene, reflecting the deep divergence of the Euteleostei (110–160 million years; Myr) [29]. [score:1]
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