sort by

8 publications mentioning bta-mir-449b

Open access articles that are associated with the species Bos taurus and mention the gene name mir-449b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 199
Other miRNAs from this paper: bta-mir-34c, bta-mir-449a, bta-mir-449c, bta-mir-449d
Several studies have indicated that miR-449b may be involved in histone deacetylation; Buurman et al. reported that up-regulation of HDAC1-3 reduces expression of miR-449 in hepatocellular carcinoma cells [20], and William et al. observed that the influenza -induced expression of miR-449b interacted with the histone deacetylase HDAC1 to alter IFN-β gene expression [21]. [score:10]
Target genes for miR-449b were predicted using the TargetScan and miRanda software, and the intersection of target genes were selected. [score:7]
According to predictions of target genes using the TargetScan and miRanda software and relative literature, c-MYC, HDAC1, BCL-2, CDK6, NANOG and CCND1 were selected as candidate targets of miR-449b. [score:7]
In the present study, HDAC1 was verified as one of the important target genes of miR-449b, suggesting that miR-449b delivered in the sperm might improve the acetylation level of histone by down -regulating the expression of HDAC1 during embryonic development and promote the reprogramming. [score:7]
In addition, the expression levels of these target genes were detected in different developmental stages of preimplantation embryos derived from IVF, NT-miR-449b (dox -induced cells as nuclear donor) and NT-control group (non -induced cells as nuclear donor). [score:6]
Kheir, T. B. et al. miR-449 inhibits cell proliferation and is down-regulated in gastric cancer. [score:6]
The relative expression levels of miR-449b target genes were examined at the 2-cell and 8-cell stages by qRT-PCR. [score:5]
In this study, we found that histone H3K9ac was significantly lower at the 2-cell and 8-cell stages in NT-control embryos, but its expression level could similar to the IVF by miR-449b overexpression in donor cells. [score:5]
Then, the expression levels of miR-449b was examined in dox -induced cells, non -induced cells and sperm, and these data were normalized to the expression of small nuclear RNA U6. [score:5]
Figure 7Relative expression levels of the target genes of miR-449b. [score:5]
Though weak expression of miR-449b was discovered in oocytes and fibroblasts, expression levels were significantly higher in sperm (Fig.   1A). [score:5]
The expression levels of BCL-2, CDK6, c-MYC, and HDAC1 were significantly higher without miR-449b overexpression (NT-control group) at both the 2-cell and 8-cell stages, but returned to the level of IVF group when donor cells were treated with dox (Fig.   7). [score:5]
Target gene predictions and relative researches indicated that BCL-2, CDK6, c-MYC, HDAC1, NANOG and CCND1 might be the target genes of miR-449b. [score:5]
Therefore, we speculated that miR-449b delivered in the sperm might improve the acetylation level of histone by down -regulating the expression of HDAC1 during embryonic development and promote epigenetic reprogramming. [score:5]
Though these regulatory mechanisms remain unclear, two cell cycle regulatory genes, CDK6 and c-MYC, were identified as the target genes of miR-449b. [score:5]
The bars graphs indicate the ratios (renilla luciferase/firely luciferase) of different putative target genes, and the result showed that the ratios of c-MYC, HDAC1, BCL-2, and CDK6 were reduced when compared with the control group, while NANOG and CCND1 were increased, suggested c-MYC, HDAC1, BCL-2, and CDK6 might be the target genes of miR-449b in vivo. [score:4]
Although miR-449b mimic could alter the expression level of miR-449b in cells, it did not exclude other endogenous miRNAs (regulate NANOG or/and CCND1) or CeRNA (binding miR-449b competitively). [score:4]
In our previous studies we found that miR-449b was highly expressed in bovine sperm [19], suggesting that it might be delivered into oocytes and participate the development of embryos after fertilization. [score:4]
Gene sequences from NCBI confirmed seed sequence of miR-449b in the 3′UTR of putative target genes, and those with 3′ UTR matches were further considered as targets. [score:4]
We noticed that during early embryonic development in bovine, miR-449b related studies were few, so we attempted to explore whether miR-449b plays a regulatory role in bovine embryonic development. [score:4]
In summary, the present results indicated that sperm-borne miR-449b may play crucial roles in early developmental regulation, specifically prior to the first cleavage division. [score:3]
Prediction and identification of target genes for miR-449b. [score:3]
Compared to PA embryos, we observed that miR-449b was more strongly expressed in IVF during early-development stage, indicating that we could exclude the interference from maternally and zygotically (see Supplementary Fig.   S2). [score:3]
Figure 1Quantitative (q) PCR analysis of relative expression levels of miR-449b in fibroblasts, oocytes, and sperm (A), as well as in no dox -induced fibroblasts, dox -induced fibroblasts, and sperm (B). [score:3]
As shown in the result, the expression of miR-449b was similar in the IVF and NT-miR449b group, but significantly higher at the 1-cell stage, indicating that induced miR-449b level in NT could be comparable to IVF zygotes, meanwhile, sperm-borne miR-449b did not degrade immediately after fertilization but remained in zygotes. [score:3]
Expression of miR-449b in oocytes, fibroblasts, sperm, dox -induced cells, and embryos derived from IVF, SCNT, and PA. [score:3]
The showed that the relative expression level of miR-449b in dox -induced cells was similar to sperm, and significantly higher than that in no dox -induced cells (Fig.   1B). [score:3]
Bovine fetal fibroblasts, expressing similar level of miR-449b as sperm through the control of Tet-On system, were used as nuclear donor cells for SCNT. [score:3]
As part of the same family, miR-34c and miR-449b share the same seed sequence and many target genes 14, 35. [score:3]
Besides, the expression pattern of miRNA-449b in bovine IVF preimplantation embryos can be found as Supplementary Fig.   S3. [score:3]
miR-449b overexpression increased global histone acetylation levels of SCNT early embryos. [score:3]
Confirmation of target genes of miR-449b in early embryos. [score:3]
miR-449b overexpression decreased apoptosis index of SCNT embryos. [score:3]
miR-449b overexpression prolonged the first cleavage time of SCNT embryo. [score:3]
Figure 2Representative images of G418-resistant colonies after transfection with Tet-On 3 G expression vector (miR-449b-pTRE3G-BI-eGFP and pEF1α-Tet3G). [score:3]
Previous studies about the function of miR-449b mainly focused on its regulatory role in the progression of cancer 14– 16. [score:2]
Buggele, W. A., Krause, K. E. & Horvath, C. M. Small RNA Profiling of Influenza A Virus-Infected Cells Identifies miR-449b as a Regulator of Histone Deacetylase 1 and Interferon Beta. [score:2]
These results indicated that sperm-born miR449b might be involved in the regulation of the timing of the first cleavage. [score:2]
Besides, the intracellular environment also affected the temporal regulation of miR-449b. [score:2]
Therefore, we postulated that sperm-born miR-34c and miR-449b might cooperate in the regulation of embryonic first cleavage. [score:2]
Further studies will be required to clarify the underlying mechanism of how these two genes regulate the first embryonic cleavage through sperm-born miR-449b. [score:2]
These results indicated that miR-449b might play pivotal roles in cell cycle regulation (CDK6 and c-MYC), cellular apoptosis (BCL-2), and epigenetic reprogramming (HDAC1). [score:2]
Development of SCNT embryos in the IVF, NT-miR-449b, and NT-control groups at 24 h, 48 h, day 6 and day 7 after activation/insemination were showed in corresponding image, respectively. [score:2]
After co-transfection of bovine fetal fibroblast cells with the response element (miR-449b-pTRE3G-BI-eGFP) and regulatory element (pEF1α-Tet3G), positive clones were selected using G418 for more than one week, and the fluorescence intensity and the growth state of cells were observed by fluorescence microscopy. [score:2]
Embryos at different development stages (2-cell, 8-cell, and blastocyst) in the IVF group, NT-miR-449b group and NT-control group were collected for immunofluorescence staining, respectively. [score:2]
We inferred that these potential RNAs in cells may be involved in the regulatory network of miR-449b through a particular way. [score:2]
Kheir et al. showed a loss of miR-449 expression in human gastric tumours compared to normal tissues [17]. [score:2]
The miR-449b expression among three groups decreased at the 2-cell stage and significantly lower in the NT-control group, however, increased significantly in IVF group at the 8-cell stage compared to the NT-control and NT-miR449b group (see Supplementary Fig.   S1). [score:2]
Briefly, cells were transfered into 4-mm cuvette gap with 10 μg of miR-449b-pTRE3G-BI-eGFP and 5 μg of pEF1α-Tet3G, and transfected using the BTX Electro Cell Manipulator ECM2001 with three pulses of 1-msec at 510 V. Then transfected cells were transferred to a 90-mm dish for culture. [score:1]
In the present study, we found that sperm-born miR-449b played important roles on cleavage timing, blastocyst formation, epigenetic reprogramming, and blastomere apoptosis. [score:1]
In the present study, we found that miR-449b influenced not only cleavage rate but also cleavage speed, i. e. the first cleavage occurred 24 h later in most of IVF and miR449b supplemented SCNT embryos, while for the NT-control embryos, the first cleavage occurred before 24 h after activation/insemination. [score:1]
Gene ontology (GO) annotations indicate that miR-449 related pathways include DNA damage response, cell cycle, and senescence and autophagy in cancer. [score:1]
The above results indicated that miR-449b may be involved in the reprogramming of histone acetylation that occurs during early post-fertilization events. [score:1]
As shown in Fig.   5A, no significant differences in the apoptosis index between the IVF and NT-miR-449b group, while apoptosis index in the NT-control blastocyst was significantly higher than both the IVF and NT-miR-449b groups (P < 0.05). [score:1]
These results indicate that miR-449b influences the first cleavage division. [score:1]
The pre-miR-449b sequence derived from miRBase (http://www. [score:1]
miR-449b is a member of miR-449 cluster composed of miR-449a, miR-449b, miR-449c and miR-449d. [score:1]
In the IVF and NT-miR-449b groups, the acetylation levels of histone H3K9 were significantly higher at the 2-cell and 8-cell stages than that of the NT-control group, and there was no difference between the NT-miR-449b and IVF embryos. [score:1]
To obtain the pre-miR-449b and the eGFP (enhanced green fluorescent protein) fragments, double enzyme digestion of the Kpn I-Pst I site of PUC57 and the BamH I-Not site of pd1EGFP-N1 (Clontech, Mountain View, CA, USA) was performed, respectively. [score:1]
However, at 48 h, the cleavage rate was significantly higher in the NT-miR-449b and IVF than NT-control group (78.02% ± 1.34, and 69.28% ± 2.54 vs 61.50% ± 3.21, P < 0.05). [score:1]
As shown in Table  1 and Fig.   3, the cleavage of the NT-control embryos was faster than IVF embryos at 24 h after activation or insemination, i. e. more than a half of NT-control embryos were 2-cell while the cleavage rate is only 35.47% ± 1.96 and 37.68% ± 2.14 in NT-miR-449b and IVF group respectively, which is significantly lower than that of NT-control group (P < 0.05). [score:1]
Meanwhile, we characterized the expression of miR-449b in 1-cell, 2-cell, 8-cell embryos derived from in vitro fertilization (IVF), SCNT, and parthenogenetic activation (PA). [score:1]
The cells of the NT-miR-449b group (dox -induced) and NT-control group (no dox -induced) were used as donor cells for nuclear transfer. [score:1]
Although, no significant difference in blastocyst formation rate on day 7 was observed among the three groups (30.36% ± 2.02 and 30.19% ± 1.67 vs 22.18% ± 2.34, P > 0.05), blastocyst formation rate on day 6 was significantly higher in the NT-miR-449b and IVF than NT-control group (30.19% ± 1.37 and 29.36% ± 2.02 vs 18.87% ± 2.34, P < 0.05). [score:1]
The levels of firefly luciferase and renilla luciferase of candidate genes were detected after miR-449b mimic/mimic control and recombinant plasmid psiCHECK [TM]-2-3′UTR were co -transfected into 293T cells. [score:1]
The pre-miR-449b and eGFP fragments were inserted into the multiple cloning site (MCS) of the pTRE3G-BI vector and yielded the final construct (miR-449b-pTRE3G-BI-eGFP). [score:1]
The result showed that the relative luciferase activity (renilla luciferase/firely luciferase) of c-MYC, HDAC1, BCL-2, and CDK6 was significantly reduced when compared with the control group (Fig.   6), indicating they were regulated by miR-449b in vivo. [score:1]
Next, miR-449b mimic/mimic control and recombinant plasmid psiCHECK [TM]-2–3′ UTR were co -transfected into 293T cells. [score:1]
[1 to 20 of 68 sentences]
2
[+] score: 22
However, of these DE miRNAs, the top three up-regulated miRNAs were bta-miR-592, bta-miR-1247-5p, bta-miR-2484, with an increased fold-change of 11.94, 4.114338, 3.319338, respectively; the top three down-regulated miRNAs were bta-miR-449a, bta-miR-34c, bta-miR-449b, with a decreased fold-change of 0.001319, 0.006306, 0.007821, respectively. [score:7]
As the essential roles played by these miRNAs in meiosis, the downregulation of bta-miR-34b/c and bta-miR-449 cluster in cattleyak could repress the expression of Nanos3, Scp3, and Stra8 and contribute to the failure of the transition from mitosis to meiosis and the subsequent spermiogensis, which was consistent with our previous finding that spermatogenic arrest of cattleyak got aggravated during meiosis [3]. [score:6]
Furthermore, the downregulation of bta-miR-34b/c and bta-miR-449 cluster may have caused the transition failure from mitosis to meiosis and the subsequent spermiogensis in cattleyak. [score:4]
Bao J MicroRNA-449 and microRNA-34b/c function redundantly in murine testes by targeting E2F transcription factor-retinoblastoma protein (E2F-pRb) pathwayJ Biol Chem. [score:3]
Upon the initiation of meiosis, the miR-449 cluster and miR-34b/c functioned redundantly in down -regulating the activities of the E2F-pRb pathway, which allowed male germ cells to exit from the mitotic cycle and to enter the meiotic program in murine testes [37]. [score:2]
[1 to 20 of 5 sentences]
3
[+] score: 5
Expression of related miRNA family members was apparent with increased expression of bta-mir-34b (log [2] FC 1.7) and bta-mir-34c (log [2] FC 1.6), as well as bta-mir-449a, bta-mir-449b and bta-mir-449c (log [2] FC 3.8, 3.4 and 3.9, respectively). [score:5]
[1 to 20 of 1 sentences]
4
[+] score: 5
Other miRNAs from this paper: bta-mir-26a-2, bta-mir-29a, bta-let-7f-2, bta-mir-16b, bta-mir-21, bta-mir-221, bta-mir-222, bta-mir-30d, bta-mir-99a, bta-mir-145, bta-mir-181a-2, bta-mir-199a-1, bta-mir-27b, bta-mir-142, bta-mir-181b-2, bta-mir-30e, bta-mir-92a-2, bta-let-7d, bta-mir-132, bta-mir-181c, bta-mir-191, bta-mir-199b, bta-mir-214, bta-mir-29b-2, bta-mir-29c, bta-mir-455, bta-let-7g, bta-mir-10b, bta-mir-24-2, bta-let-7a-1, bta-mir-150, bta-let-7f-1, bta-mir-122, bta-let-7i, bta-mir-34c, bta-mir-363, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-195, bta-mir-34a, bta-mir-365-1, bta-mir-99b, bta-mir-100, bta-mir-129-1, bta-mir-129-2, bta-mir-130a, bta-mir-130b, bta-mir-133a-2, bta-mir-133a-1, bta-mir-143, bta-mir-146b, bta-mir-146a, bta-mir-155, bta-mir-181d, bta-mir-182, bta-mir-183, bta-mir-184, bta-mir-24-1, bta-mir-196a-2, bta-mir-196a-1, bta-mir-199a-2, bta-mir-212, bta-mir-26a-1, bta-mir-28, bta-mir-29d, bta-mir-32, bta-mir-335, bta-mir-338, bta-mir-339a, bta-mir-346, bta-mir-365-2, bta-mir-378-1, bta-mir-383, bta-mir-409a, bta-mir-449a, bta-mir-449c, bta-mir-592, bta-mir-708, bta-mir-92a-1, bta-mir-92b, bta-mir-29e, bta-mir-29b-1, bta-mir-1271, bta-mir-1249, bta-mir-181a-1, bta-mir-181b-1, bta-mir-2285a, bta-mir-2285d, bta-mir-2285b-1, bta-mir-2332, bta-mir-199c, bta-mir-2389, bta-mir-2285c, bta-mir-2404-1, bta-mir-449d, bta-mir-2411, bta-mir-2446, bta-mir-339b, bta-mir-2404-2, bta-mir-2483, bta-mir-424, bta-mir-378-2, bta-mir-409b, bta-mir-2285e-1, bta-mir-2285e-2, bta-mir-2285f-1, bta-mir-2285f-2, bta-mir-2285g-1, bta-mir-2285h, bta-mir-2285i, bta-mir-2285j-1, bta-mir-2285j-2, bta-mir-2285k-1, bta-mir-2285l, bta-mir-2285o-1, bta-mir-2285o-2, bta-mir-2285n-1, bta-mir-2285n-2, bta-mir-2285p, bta-mir-2285m-1, bta-mir-2285m-2, bta-mir-378b, bta-mir-2285n-3, bta-mir-2285n-4, bta-mir-2285o-3, bta-mir-2285o-4, bta-mir-2285m-3, bta-mir-378c, bta-mir-2285m-4, bta-mir-2285o-5, bta-mir-2285m-5, bta-mir-2285n-5, bta-mir-2285n-6, bta-mir-2285n-7, bta-mir-2285k-2, bta-mir-2285k-3, bta-mir-2285k-4, bta-mir-2285k-5, bta-mir-2285q, bta-mir-2285r, bta-mir-2285s, bta-mir-2285t, bta-mir-2285b-2, bta-mir-2285v, bta-mir-2285g-2, bta-mir-2285g-3, bta-mir-2285af-1, bta-mir-2285af-2, bta-mir-2285y, bta-mir-2285w, bta-mir-2285x, bta-mir-2285z, bta-mir-2285u, bta-mir-2285aa, bta-mir-2285ab, bta-mir-2285ac, bta-mir-2285ad, bta-mir-2285ae, bta-mir-378d, bta-mir-2285ag, bta-mir-2285ah, bta-mir-2285ai, bta-mir-2285aj, bta-mir-2285ak, bta-mir-2285al, bta-mir-2285am, bta-mir-2285ar, bta-mir-2285as-1, bta-mir-2285as-2, bta-mir-2285as-3, bta-mir-2285at-1, bta-mir-2285at-2, bta-mir-2285at-3, bta-mir-2285at-4, bta-mir-2285au, bta-mir-2285av, bta-mir-2285aw, bta-mir-2285ax-1, bta-mir-2285ax-2, bta-mir-2285ax-3, bta-mir-2285ay, bta-mir-2285az, bta-mir-2285an, bta-mir-2285ao-1, bta-mir-2285ao-2, bta-mir-2285ap, bta-mir-2285ao-3, bta-mir-2285aq-1, bta-mir-2285aq-2, bta-mir-2285ba-1, bta-mir-2285ba-2, bta-mir-2285bb, bta-mir-2285bc, bta-mir-2285bd, bta-mir-2285be, bta-mir-2285bf-1, bta-mir-2285bf-2, bta-mir-2285bf-3, bta-mir-2285bg, bta-mir-2285bh, bta-mir-2285bi-1, bta-mir-2285bi-2, bta-mir-2285bj-1, bta-mir-2285bj-2, bta-mir-2285bk, bta-mir-2285bl, bta-mir-2285bm, bta-mir-2285bn, bta-mir-2285bo, bta-mir-2285bp, bta-mir-2285bq, bta-mir-2285br, bta-mir-2285bs, bta-mir-2285bt, bta-mir-2285bu-1, bta-mir-2285bu-2, bta-mir-2285bv, bta-mir-2285bw, bta-mir-2285bx, bta-mir-2285by, bta-mir-2285bz, bta-mir-2285ca, bta-mir-2285cb, bta-mir-2285cc, bta-mir-2285cd, bta-mir-2285ce, bta-mir-2285cf, bta-mir-2285cg, bta-mir-2285ch, bta-mir-2285ci, bta-mir-2285cj, bta-mir-2285ck, bta-mir-2285cl, bta-mir-2285cm, bta-mir-2285cn, bta-mir-2285co, bta-mir-2285cp, bta-mir-2285cq, bta-mir-2285cr-1, bta-mir-2285cr-2, bta-mir-2285cs, bta-mir-2285ct, bta-mir-2285cu, bta-mir-2285cv-1, bta-mir-2285cv-2, bta-mir-2285cw-1, bta-mir-2285cw-2, bta-mir-2285cx, bta-mir-2285cy, bta-mir-2285cz, bta-mir-2285da, bta-mir-2285db, bta-mir-2285dc, bta-mir-2285dd, bta-mir-2285de, bta-mir-2285df, bta-mir-2285dg, bta-mir-2285dh, bta-mir-2285di, bta-mir-2285dj, bta-mir-2285dk, bta-mir-2285dl-1, bta-mir-2285dl-2, bta-mir-2285dm
Moreover, the expression of 13 miRNA families including bta-miR-29 (a, b, c, d), bta-miR-449 (a, b, c), bta-miR-181 (a, b, c, d), bta-miR-455 (-3p,-5p), bta-miR-99 (b, a-5p) and bta-miR-2483 (-5p,-3p) were co -overexpressed or co-repressed at day 7 compared to day 3 (Table 2). [score:4]
Among miRNAs increased at day 7 of the estrous cycle, bta-miR-2389, bta-miR-29d, bta-miR-363, bta-miR-1249, bta-miR-338, bta-miR-129-3p, bta-miR-129-5p, bta-miR-129, bta-miR-142-3p, bta-miR-449b, bta-miR-2285t and bta-miR-346 were not detected at day 3 (Figure 9A). [score:1]
[1 to 20 of 2 sentences]
5
[+] score: 4
Other miRNAs from this paper: bta-mir-125a, bta-mir-125b-1, bta-mir-128-1, bta-mir-181a-2, bta-mir-199a-1, bta-mir-27b, bta-mir-34b, bta-mir-127, bta-mir-181b-2, bta-mir-215, bta-mir-218-2, bta-mir-30e, bta-mir-181c, bta-mir-192, bta-mir-200a, bta-mir-200c, bta-mir-22, bta-mir-30a, bta-mir-200b, bta-mir-122, bta-mir-34c, bta-mir-125b-2, bta-mir-34a, bta-mir-128-2, bta-mir-143, bta-mir-146b, bta-mir-154a, bta-mir-181d, bta-mir-199a-2, bta-mir-218-1, bta-mir-32, bta-mir-326, bta-mir-429, bta-mir-449a, bta-mir-449c, bta-mir-504, bta-mir-181a-1, bta-mir-181b-1, bta-mir-2285a, bta-mir-2285d, bta-mir-2285b-1, bta-mir-2285c, bta-mir-449d, bta-mir-424, bta-mir-2285e-1, bta-mir-2285e-2, bta-mir-2285f-1, bta-mir-2285f-2, bta-mir-2285g-1, bta-mir-2285h, bta-mir-2285i, bta-mir-2285j-1, bta-mir-2285j-2, bta-mir-2285k-1, bta-mir-2285l, bta-mir-2285o-1, bta-mir-2285o-2, bta-mir-2285n-1, bta-mir-2285n-2, bta-mir-2285p, bta-mir-2285m-1, bta-mir-2285m-2, bta-mir-2285n-3, bta-mir-2285n-4, bta-mir-154c, bta-mir-154b, bta-mir-2285o-3, bta-mir-2285o-4, bta-mir-2285m-3, bta-mir-2285m-4, bta-mir-2285o-5, bta-mir-2285m-5, bta-mir-2285n-5, bta-mir-2285n-6, bta-mir-2285n-7, bta-mir-2285k-2, bta-mir-2285k-3, bta-mir-2285k-4, bta-mir-2285k-5, bta-mir-2285q, bta-mir-2285r, bta-mir-2285s, bta-mir-2285t, bta-mir-2285b-2, bta-mir-2285v, bta-mir-2285g-2, bta-mir-2285g-3, bta-mir-2285af-1, bta-mir-2285af-2, bta-mir-2285y, bta-mir-2285w, bta-mir-2285x, bta-mir-2285z, bta-mir-2285u, bta-mir-2285aa, bta-mir-2285ab, bta-mir-2285ac, bta-mir-2285ad, bta-mir-2285ae, bta-mir-2285ag, bta-mir-2285ah, bta-mir-2285ai, bta-mir-2285aj, bta-mir-2285ak, bta-mir-2285al, bta-mir-2285am, bta-mir-2285ar, bta-mir-2285as-1, bta-mir-2285as-2, bta-mir-2285as-3, bta-mir-2285at-1, bta-mir-2285at-2, bta-mir-2285at-3, bta-mir-2285at-4, bta-mir-2285au, bta-mir-2285av, bta-mir-2285aw, bta-mir-2285ax-1, bta-mir-2285ax-2, bta-mir-2285ax-3, bta-mir-2285ay, bta-mir-2285az, bta-mir-2285an, bta-mir-2285ao-1, bta-mir-2285ao-2, bta-mir-2285ap, bta-mir-2285ao-3, bta-mir-2285aq-1, bta-mir-2285aq-2, bta-mir-2285ba-1, bta-mir-2285ba-2, bta-mir-2285bb, bta-mir-2285bc, bta-mir-2285bd, bta-mir-2285be, bta-mir-2285bf-1, bta-mir-2285bf-2, bta-mir-2285bf-3, bta-mir-2285bg, bta-mir-2285bh, bta-mir-2285bi-1, bta-mir-2285bi-2, bta-mir-2285bj-1, bta-mir-2285bj-2, bta-mir-2285bk, bta-mir-2285bl, bta-mir-2285bm, bta-mir-2285bn, bta-mir-2285bo, bta-mir-2285bp, bta-mir-2285bq, bta-mir-2285br, bta-mir-2285bs, bta-mir-2285bt, bta-mir-2285bu-1, bta-mir-2285bu-2, bta-mir-2285bv, bta-mir-2285bw, bta-mir-2285bx, bta-mir-2285by, bta-mir-2285bz, bta-mir-2285ca, bta-mir-2285cb, bta-mir-2285cc, bta-mir-2285cd, bta-mir-2285ce, bta-mir-2285cf, bta-mir-2285cg, bta-mir-2285ch, bta-mir-2285ci, bta-mir-2285cj, bta-mir-2285ck, bta-mir-2285cl, bta-mir-2285cm, bta-mir-2285cn, bta-mir-2285co, bta-mir-2285cp, bta-mir-2285cq, bta-mir-2285cr-1, bta-mir-2285cr-2, bta-mir-2285cs, bta-mir-2285ct, bta-mir-2285cu, bta-mir-2285cv-1, bta-mir-2285cv-2, bta-mir-2285cw-1, bta-mir-2285cw-2, bta-mir-2285cx, bta-mir-2285cy, bta-mir-2285cz, bta-mir-2285da, bta-mir-2285db, bta-mir-2285dc, bta-mir-2285dd, bta-mir-2285de, bta-mir-2285df, bta-mir-2285dg, bta-mir-2285dh, bta-mir-2285di, bta-mir-2285dj, bta-mir-2285dk, bta-mir-2285dl-1, bta-mir-2285dl-2, bta-mir-2285dm
Of these 85 differentially expressed miRNAs, we detected six miRNA families, including miR-2285, miR-34, miR-192, miR-449, miR-200 families (Table S4). [score:3]
The miR-34 family consist of four members, including miR-34a, miR-34b, miR-449a, miR-449b. [score:1]
[1 to 20 of 2 sentences]
6
[+] score: 1
The simultaneous inactivation of miR-34b/c and its functionally related family member miR-449 - which contains an identical seed sequence, result in male sterility in mice due to severely altered epididymis as well as low sperm counts and deformed sperm with minimal motility [30]. [score:1]
[1 to 20 of 1 sentences]
7
[+] score: 1
Besides the abovementioned miRNAs promoting myoblast differentiation, a few identified molecules such as miR-29b [49], miR-31 [50], miR-9 [51], miR-145 [52], miR-194 [53], miR-378 [54], miR-449 [55], miR-503 [11, 27], miR-542, [56], and miR-660 [11] were described in the literature as skeletal muscle-related. [score:1]
[1 to 20 of 1 sentences]
8
[+] score: 1
While, 36 miRNAs including bta-miR-409a and bta-miR-449b were unique to subordinate follicles. [score:1]
[1 to 20 of 1 sentences]