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15 publications mentioning ssc-mir-124a-1

Open access articles that are associated with the species Sus scrofa and mention the gene name mir-124a-1. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 126
The suspected target gene was Sox9 and embryonic overexpression of mir-124 lead to overexpression of this target gene in the adult mouse, presumably to compensate for the abnormal embryonic miRNA profile [55]. [score:9]
In abdominal fat the expression of both mir-9 and mir-124a was found to be more highly expressed in obese males (mir-124a FC: 119.8; p-value = 0.0108, mir-9 FC: 7.8; p-value = 0.07) and both were significantly more highly expressed in the liver of obese males (mir-124a FC: 12.3; p-value = 0.013, mir-9 FC: 1.6; p. value = 0.036) while no difference in expression could be detected in the females. [score:9]
A selection of target genes for mir-9 and mir-124 (list in S7 Dataset) were tested by qPCR but none of them were significantly suppressed in the obese group (S8 Dataset). [score:5]
qPCR validation of these results in general confirms the expression pattern, however, statistical significant differential expression was only observed for two of the six miRNAs, mir-9 and mir-124a (Table 6, Fig 2). [score:5]
Two of the 6 miRNAs found to be differentially expressed in the sequencing study, mir-9 and mir-124a, were significantly differentially expressed between the two groups (Table 6, Fig 2). [score:5]
In Fig 2 the expression of mir-9 and mir-124a in lean and obese subcutaneous adipose tissue is illustrated in a Column scatter plot as the relative mean of log2 fold change were the lowest expressed sample of each miRNA assay is set to 1. Mir-9 and mir-124a are both significantly up regulated in obese animals with even higher fold changes than found in the sequencing study. [score:5]
MicroRNAs miR-96, miR-124, and miR-199a regulate gene expression in human bone marrow-derived mesenchymal stem cells. [score:4]
In a study of macrophages in lung inflammation, mir-124 expression was up regulated in macrophages by IL4 and IL13 stimulation as well as by allergy induced inflammation [53]. [score:4]
Expression of mir-9 and mir-124a in Subcutaneous Adipose Tissue. [score:3]
The data is presented as column scatter plots in Fig 3. Mir-9 and mir-124a were expressed in all three tissues. [score:3]
On the other hand, adipocytes have also been proven to express mir-124 [54]. [score:3]
Fold changes and p-values for qPCR in subcutaneous adipose tissue can be found in Table 6. To further study the expression of mir-9 and mir-124a, the two miRNAs with the largest fold changes between the lean and obese group in the subcutaneous adipose tissue, qPCR was also performed on cDNA from abdominal adipose tissue, liver and muscle from the same animals as in the study of subcutaneous adipose tissue. [score:3]
IL-4/IL-13 -dependent and independent expression of miR-124 and its contribution to M2 phenotype of monocytic cells in normal conditions and during allergic inflammation. [score:3]
The overexpression of mir-9 and mir-124 in the adipose tissue of obese pigs may also contribute to the lipid accumulation in the adipocytes. [score:3]
Additionally, only mir-124 is significantly up regulated in abdominal adipose tissue of male obese pigs and both mir-9 and mir-124a are up regulated in the liver of obese male pigs. [score:3]
0131650.g002 Fig 2 qPCR expression of mir-9 (A) and mir-124a (B) in Subcutaneous Adipose Tissue (SC AT). [score:3]
qPCR expression of mir-9 and mir-124a in Abdominal Adipose Tissue (Abd AT) (A,B) and Liver (C,D). [score:3]
Among these the expression profiles of mir-124a-3p and mir-9-3p overlapped with the results for the analysis of the combined dataset. [score:3]
Inactivated lipid carrying HSCs have higher mir-9 and mir-124 expression than activated HSCs that carry no lipids [63]. [score:3]
Student’s t-test of mir-9 and mir-124a qPCR Expression in Liver and Abdominal Adipose Tissue. [score:3]
Mir-9 and mir-124a were both significantly differentially expressed in the liver of obese males, while there was no significant difference in the females. [score:3]
MiRTarbase Verified mir-9 and mir-124 Target Genes. [score:3]
In abdominal adipose tissue both mir-9 and mir-124a was expressed at a higher level in the obese male pigs, (p-value = 0.07 and 0.01 respectively). [score:3]
QPCR studies confirm some of these differences, in particular for mir-9 and mir-124a which are significantly differentially expressed with large fold changes. [score:3]
qPCR expression of mir-9 (A) and mir-124a (B) in Subcutaneous Adipose Tissue (SC AT). [score:3]
In the present study, where all cells in the adipose tissue have been included for the extraction of RNA, it might be that the increased mir-124a expression in this tissue is due to the presence of macrophages. [score:3]
0131650.g003 Fig 3 qPCR expression of mir-9 and mir-124a in Abdominal Adipose Tissue (Abd AT) (A,B) and Liver (C,D). [score:3]
Expression of mir-9 and mir-124a in Abdominal Adipose Tissue and Liver. [score:3]
When the qPCR results were analyzed for male pigs only, mir-99a was differentially expressed in addition to mir-124a and mir-9 (Table 6). [score:3]
In the female pigs mir-9, mir-124a, mir-103, mir-10b and mir-99a were differentially expressed (Table 6). [score:3]
Analysis of mir-9 and mir-124a target genes has not previously been performed in adipose tissue. [score:3]
Differential expression in the sequencing data from the lean and obese pigs was calculated using the DESeq2 package in R and revealed six significantly differentially expressed miRNAs between the two groups: mir-9-5p, mir-124a-3p, mir-9-3p, mir-199a-5p, mir-489-3p and mir-34c-3p. [score:3]
Mir-9 and mir-124a are significantly up regulated in subcutaneous adipose tissue of obese pigs, independently of gender. [score:2]
Mir-124 expression has previously been linked to weight gain. [score:2]
Mir-124 is also targeting ADIPOR2 –a receptor for the protein hormone adiponectin, which is secreted in adipose tissue [58]. [score:2]
This is, to our knowledge, the first study of subcutaneous adipose tissue of lean versus obese subjects where mir-9 and mir-124a have been shown to be significantly up regulated in the obese subjects with large fold changes compared to the lean subjects. [score:1]
An example is that mir-9 and mir-124 are slightly up regulated in the blood of diabetic patients compared to non-diabetic controls [52]. [score:1]
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2
[+] score: 85
Use of miR-124 target sites restricts transgene expression to murine RPE in vivo To restrict AAV2/5-CMV -mediated transgene expression to the RPE, we exploited miR-124, a miRNA abundantly expressed in differentiated neurons [30]. [score:9]
To check whether the high number of miRTs could perturb the capacity of miRNAs to regulate their physiological target genes, we analyzed the expression levels of VAMP3 and RDH10, two direct targets of miR-124 [39], [40] in retinal samples of animals (n = 4) injected with high doses of either the control AAV2/5-CMV- EGFP or the construct bearing the miR-124 binding sites. [score:9]
In particular, constructs harboring miR-124 binding sites can efficiently de-target reporter expression from the PRs, while the presence of miR-204 sites induces elimination of transgene expression from the RPE. [score:7]
Finally, to exclude that the presence of exogenous miRNA target sequences can interfere with the physiological function of the PRs, we performed electroretinograms (ERG) on mice injected at a high dose (2.6×10 [9] GC/eye) with the AAV2/5 vectors harboring the miR-124 or miR-204 target sequences and the control EGFP construct. [score:5]
Although this does not appear to be a problem as we did not observe any retinal toxic effect so far [21], ideally we could have tailored RPE65 expression to RPE if we had included miR-124 target sites in our AAV vector. [score:5]
Our results demonstrate that miR-124 and miR-204 target sequences can efficiently restrict AAV2/5 -mediated transgene expression to retinal pigment epithelium and photoreceptors, respectively, in mice and pigs. [score:5]
As shown in Figure 3, the use of target sequences for miR-204 (Figures 3c,d ) and miR-124 (Figures 3e,f ) efficiently restricted AAV2/5 mediated EGFP expression to the PRs and RPE of the porcine retina, respectively. [score:5]
Use of miR-124 target sites restricts transgene expression to murine RPE in vivo. [score:5]
To this end, we generated AAV2/5 vectors expressing EGFP and containing four tandem copies of miR-124 or miR-204 complementary sequences in the 3′UTR of the transgene expression cassette. [score:5]
To restrict AAV2/5-CMV -mediated transgene expression to the RPE, we exploited miR-124, a miRNA abundantly expressed in differentiated neurons [30]. [score:5]
The latter is strongly expressed in the neural retina, predominantly in PRs [25], [26], and, to our current knowledge, is unlikely to either be under the direct control of any of the miRNAs tested or have affinity for the miR-204 and miR-124 miRTs. [score:4]
In particular, we analyzed the expression levels of miR-204 and miR-124, as well as of miR-182. [score:3]
miR-124 and miR-204 expression in retina supports the use of AAV vectors harboring corresponding miRTs. [score:3]
Given the highly conserved cellular distribution of these two miRNAs across species [26], [34], [35], we assumed that miR-124 and miR-204 are likely to be expressed in the same porcine retinal layers. [score:3]
miR-124, a neuronal-specific miRNA, is expressed in all layers of the neural retina but is not detected in the RPE. [score:3]
0022166.g001 Figure 1(a) Expression profile of miR-204 and miR-124 in retina sections of adult, albino (CD1) mouse as revealed by ISH using LNA -modified probes. [score:3]
To generate the standard curve, serial amounts (ranging from 10 [2] to 10 [8] copies) of a synthetic RNA oligonucleotide corresponding to miR-124 (5′-UAAGGCACGCGGUGAAUGCC-3′; Sigma–Aldrich, St. [score:1]
Similarly, the cassette containing four copies of a sequence which is perfectly complementary to miR-124 (in capital letters) was constructed by annealing the following two sets of oligonucleotides: 5′-ctagatctGGCATTCACCGCGTGCCTTAcgatGGCATTCACCGCGTGCCTTAaagctt-3′, 5′-TAAGGCACGCGGTGAATGCCatcgTAAGGCACGCGGTGAATGCCagat-3′ and ′-GGCATTCACCGCGTGCCTTAtcacGGCATTCACCGCGTGCCTTAagatc-3′5, 5′-tcgagatctTAAGGCACGCGGTGAATGCCgtgaTAAGGCACGCGGTGAATGCCaagctt-3′. [score:1]
The mature sequence of miR-124 and miR-204 is identical in pigs and mouse (miRBase, http://www. [score:1]
Therefore, we cloned four tandem copies of a sequence that is perfectly complementary to the mature miR-124 downstream of the WPRE element in the pAAV2.1-CMV- EGFP plasmid (Figure 1b ). [score:1]
Four tandem copies (4xmiRT) of a sequence perfectly complementary to the sequence of the mature miR-124 or miR-204 (see alignments) were cloned downstream of the WPRE element in the pAAV2.1-CMV- EGFP plasmid. [score:1]
We and others have shown by ISH that miR-124 stains strongly all retinal cell layers, but is not detected in the RPE [26], [31] (Figure 1a ). [score:1]
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3
[+] score: 11
of target genes P-value IPA Z-score Reference (DOI) Transcription Regulator NUPR1 Cell cycle 166 3.09E-14 −3.035 IPA Knowledge database PML Regulation of the TGF-beta signaling pathway 58 2.22E-12 −3.195 IPA Knowledge database CDKN2A Cell cycle negative regulator 100 3.37E-11 −2.147 IPA Knowledge database KLF3 Multicellular organismal development 112 1.48E-10 −7.504 IPA Knowledge database SMAD7 Negative regulation of BMP signaling pathway, negative regulation of cell migration 53 8.29E-10 −4.682 IPA Knowledge database KDM5A Chromatin modification, chromatin organization 54 2.02E-09 −5.900 IPA Knowledge database KDM5B Chromatin modification 55 3.34E-09 −4.673 IPA Knowledge database SPDEF Germ cell migration 33 3.00E-08 −4.402 IPA Knowledge database IRF4 Interferon-gamma -mediated signaling pathway 46 1.48E-04 −4.662 IPA Knowledge database MXI1 Negatively regulate MYC function 10 1.51E-04 −2.919 IPA Knowledge database Mature MicroRNA miR-124-3p Potential regulator of PIM1 118 1.36E-28 −10.788 Deng et al. 2016 (10.1111/cas. [score:11]
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4
[+] score: 9
There were 17 DEMs, represented by miR-124 and miR-214, dominating the miRNA-regulated gene expression in the early metaphase of embryonic stage and 10 DEMs, miR-29 for instance, were highly expressed at the postnatal stage. [score:6]
Three category patterns from the DEMs were clustered based on the expression percentages including early-middle embryonic stage from miR-124 to miR-424, late embryonic stage from miR-345 to miR-451 and adult stage from miR-29b to miR-133a, respectively. [score:3]
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5
[+] score: 9
Other miRNAs from this paper: ssc-mir-124a-2, ssc-mir-155
The expression levels of two miRNAs (mir-124 and mir-155) with over-represented targets were quantified by Stem-loop TaqMan Assay (Figure 7). [score:4]
While the expression of mir-124 in peripheral blood was almost undetectable (data not shown), miR-155 was down regulated in pig peripheral blood RNA from PS3 animals inoculated with ST (over 5 fold, p<0.01, Figure 7). [score:4]
The expression level of miR-124 and -155 were quantified in the LS3 and PS3 animals using the Stem-loop TaqMan MicroRNA Assay (ABI Assay: 000446 and 002623) and normalized by the total amount of input RNA. [score:1]
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6
[+] score: 8
Ssc-miR-124a was found to target the NP genes isolated in all 38 different times, while ssc-miR-145 targeted the NP genes isolated at 35 different times and ssc-miR-136 targeted the NA and NP genes isolated in 31 of the 38 times. [score:7]
In addition, the interaction of ssc-miR-124a and NP, and ssc-miR-145 and NP were not predicted in our work, which might be due to the different prediction tools and criteria, or the sequence-specificity of SIV-H1N1/2009. [score:1]
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7
[+] score: 6
Recently, miR-155-5p was reported to be upregulated after plaque rupture for 0.5 h and 1 h [25], and miR-124, highly expressed in monocyte/macrophage cells, was as well a promising biomarker for AMI diagnosis [10, 19], suggesting that monocytes-released miRNAs could be useful for AMI early diagnosis. [score:6]
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8
[+] score: 5
Moreover, a single intraperitoneal injection of SFV4-miR-124 into mice with implanted CT-2A orthotopic gliomas showed significant inhibition of tumor growth and improved survival rates. [score:3]
An interesting approach comprises the introduction of micro RNA-124 (miR-124) into an SFV4 vector [90]. [score:1]
As IFN-1 tolerance has been associated with the SFV nsP3-nsP4 genes, conditionally replicating SFV4-miR-124 virus was able to replicate in neurons and allowed targeting of gliomas otherwise sensitive to IFN-1. Evaluation of CT-2A mouse astrocytoma cells and IFN-1 pretreated human glioblastoma cells showed increased oncolytic potency. [score:1]
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9
[+] score: 5
A recent study has shown that the in vivo administration of miR-124 suppresses experimental autoimmune encephalitis by affecting macrophages, suggesting that miRNA delivery could be used to treat some inflammatory diseases associated with microglial activation [19]. [score:5]
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10
[+] score: 5
GO annotation was performed for the target genes of five designated differentially expressed miRNAs (miR-146b, miR-155–5p, miR-195, miR-124a and miR-1306–5p). [score:5]
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11
[+] score: 4
Li H., Xie S., Liu M., Chen Z., Liu X., Wang L., Li D., and Zhou Y. 2014 The clinical significance of downregulation of mir-124–3p, mir-146a-5p, mir-155–5p and mir-335–5p in gastric cancer tumorigenesis. [score:4]
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12
[+] score: 4
Laine S. K. Alm J. J. Virtanen S. P. Aro H. T. Laitala-Leinonen T. K. MicroRNAs miR-96, miR-124 and miR-199a regulate gene expression in human bone marrow-derived mesenchymal stem cellsJ. [score:4]
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13
[+] score: 3
Of these, targeting by the three miRNAs miR-124a, miR-136, and miR-145 appears to have been evolutionarily conserved, suggesting that these miRNA: viral gene interactions are an essential part of influenza pathogenesis. [score:3]
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14
[+] score: 3
0024883.g003 Figure 3 The selected miRNAs for the analysis include: miR-99b, miR-204, miR-27a, miR-24, miR-7, miR-145, miR-124, miR-21, miR-125b, miR-30b, miR-128a, miR-122, miR-183, and miR-103. [score:1]
0024883.g002 Figure 2 The selected miRNAs for the analysis include: miR-99b, miR-204, miR-27a, miR-24, miR-7, miR-145, miR-124, miR-21, miR-125b, miR-30b, miR-128a, miR-122, miR-183, and miR-103. [score:1]
The selected miRNAs for the analysis include: miR-99b, miR-204, miR-27a, miR-24, miR-7, miR-145, miR-124, miR-21, miR-125b, miR-30b, miR-128a, miR-122, miR-183, and miR-103. [score:1]
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15
[+] score: 1
For example, Hamrick et al., (2010) reported that miR-7, miR-468, miR-542, and miR-698 levels in mouse muscle tissue substantially increased with age, whereas miR-124a, miR-181a, miR-221, miR-382, miR-434, and miR-455 levels substantially decreased with age. [score:1]
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