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1 publications mentioning crm-mir-64b

Open access articles that are associated with the species Caenorhabditis remanei and mention the gene name mir-64b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 30
Three of the six miRNAs conserved between C. remanei and C. elegans (mir-248, mir-784, and mir-787) are located on the X chromosome and five of them (mir-64, mir-248, mir-356, mir-784, and mir-787) regulate target genes enriched in gonad formation in C. elegans. [score:4]
Tandem duplicates and gene loss within the mir-64 cluster may modulate miRNA dosage to strengthen gene expression regulation according to species-specific needs (Doench and Sharp 2004). [score:4]
In addition, our analysis of the mir-64 cluster of miRNA genes further implicates mir-64c as a direct target of positive selection. [score:4]
Extensive sequence differences in the hairpin and in the mature sequences of mir-64 paralogs suggest possible subfunctionalization following their origin by duplication (fig. 7 B), although conservation of the seed in all members implies an overlapping set of target genes. [score:3]
Interestingly, the first ranked functional clusters of five miRNAs (mir-64, mir-248, mir-356, mir-784, and mir-787) are enriched for GO terms related to gonad development, although enrichment is significant only for mir-248, mir-356, and mir-787 after correction for multiple testing (supplementary table S5, online). [score:2]
We observed this process in the mir-64 cluster as an ongoing evolutionary dynamic between the closely related species C. remanei and C. latens, and within and between populations of C. remanei for three other miRNAs. [score:1]
Mature sequences of mir-64 homologs are well conserved between C. remanei and C. latens, with the exception of mir-64c homologs, which differ by up to three substitutions between the two species. [score:1]
We reconstructed the phylogenetic relationships among mature sequences of members of the mir-64 cluster in C. remanei, C. latens, C. elegans, C. briggsae, and C. brenneri using Neighbor-Joining with a maximum composite likelihood distance in MEGA 5, and assessed node confidence with 1,000 bootstrap replicates. [score:1]
To better understand the very recent evolution of the mir-64 cluster, we then examined the pattern of genetic differentiation of mir-64 miRNAs between populations of C. remanei. [score:1]
We could not compare diversity between flanking sequences and miRNA hairpins for members of the mir-54 and mir-64 clusters. [score:1]
We identified an extra mir-64 copy within the C. latens mir-64 cluster based on sequence similarity with mir-64 homologs in C. latens and C. remanei, and typical minimum free energy stem-loop structure computed with the Vienna RNA server. [score:1]
The number of mir-64 paralogs differs between C. remanei and C. latens because of the possible loss of mir-64c-1 in C. remanei. [score:1]
We found that evolution of the mir-64 cluster is particularly dynamic. [score:1]
Two other miRNAs (lin-4 and mir-64b) have an SNP in the seed motif of the star miRNA, although we observed no substitutions between C. remanei and C. latens orthologs in the seeds of mature and star miRNAs (fig. 1 A). [score:1]
Nevertheless, we also showed extensive sequence divergence in the hairpin and mature sequences of mir-64 paralogs in both C. remanei and C. latens. [score:1]
However, the number of mir-64 paralogs varies up to 2-fold because of the independent gain and/or loss of tandem duplicates in different Caenorhabditis species. [score:1]
We also computed nucleotide divergence, with a maximum composite likelihood distance, among mir-64 homologs in C. remanei and C. latens separately for the mature and hairpin sequences. [score:1]
We included four additional strains of C. remanei (Japan; Tennessee, United States) and one of C. latens (JU724 from Zhouzhuang City, China) for analysis of the mir-64 cluster. [score:1]
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