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3 publications mentioning bta-mir-2305

Open access articles that are associated with the species Bos taurus and mention the gene name mir-2305. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 53
The dual luciferase reporter system was then used to validate the target genes described in Fig 7. To determine whether bta-let-7i and bta-miR-2305 can directly target their predicted target genes, including MAP3K1, proheparin -binding epidermal growth factor-like growth factor (HBEGF), serine/threonine-protein kinase (PAK1) and Ras-related C3 botulinum toxin substrate 2 (RAC2), we designed luciferase reporter constructs that included either the wild- or mutant-type 3’-UTR of MAP3K1, HBEGF, PAK1 and RAC2 (Fig 7C). [score:8]
Regarding qRT-PCR used validate relative gene expression of the 8 selected miRNAs, we showed in Fig 5E that miR-let-7i, miR-2305 and miR-2890 were upregulated and the expression of miR-874, miR-1, miR-106b, miR-1296 and miR-362-5p decreased in steers. [score:8]
The effects of overexpression or suppression of bta- let-7i or bta-miR-2305 on the levels of mRNA expression of endogenous MAP3K1, HBEGF, PAK1 and RAC2 were further examined. [score:7]
Interestingly, our data on targets validation provided strong evidence that MAP3K1 and HBEGF are targets of bta-let-7i, whereas PAK1 and RAC2 are targets of bta-miR-2305 (Fig 7C, 7D, 7E, 7F and 7G). [score:7]
G: Effect of bta-miR-2305 -mimics, mimic-NC or bta-miR-2305 -inhibitor, inhibitor-NC on PAK1 and RAC2 mRNA levels in bovine pre-adipocytes. [score:5]
These results demonstrated that MAP3K1 and HBEGF are targets of bta-let-7i, whereas PAK1 and RAC2 are targets of bta-miR-2305. [score:5]
Of note, co-transfection with bta-let-7i and bta-miR-2305 significantly suppressed luciferase activity (Fig 7D and 7E), and wild-type MAP3K1, HBEGF, PAK1, RAC2 groups had approximately 48% (P<0.01), 33% (P<0.01), 22% (P<0.05) and 17% (P<0.05) less luciferase activity, respectively, than the corresponding NC groups. [score:3]
Chemically synthetic bta- let-7i and bta-miR-2305 mimics or inhibitor and the negative control (purchased from Ribobio, Guangzhou, China) were transfected into 293A or primary pre-adipocyte cells using a standard reverse transfection protocol at 50 nM. [score:3]
The qRT-PCR results showed that bta- let-7i or bta-miR-2305 could markedly affect the expression of MAP3K1, HBEGF, PAK1 and RAC2 mRNA (P<0.05; Fig 7F and 7G). [score:3]
Bovine pre-adipocytes were transfected with bta- let-7i or bta-miR-2305 mimics, inhibitor and NC. [score:3]
D, E: Effect of bta- let-7i–mimic or bta-miR-2305 mimic and its mimic-NC on luciferase activity in 293A cells transfected with either the WT 3’UTR or 3’UTR-Mut reporter. [score:1]
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2
[+] score: 2
It is especially notable that the bovine-specific miR-1584-5p, miR-2305, miR-2412, and miR-2478, most of which are associated with energy metabolism [32], were highly enriched in the plasma samples of both groups in the present study. [score:1]
Those profiles were enriched with bovine-specific miR-1584-5p, miR-2305, miR-2412, and miR-2478, which are associated with energy metabolism [32]. [score:1]
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3
[+] score: 1
Also present were miR-1777b (6.71%), miR-1777a (4.88%), miR-1246 (4.42%), miR-126-3p (2.44%), miR-2305 (2.07%), miR-1584-5p (1.90%), miR-2413 (1.74%), miR-4286 (1.58%), miR-1224 (1.56%), and miR-451 (1.41%). [score:1]
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