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2 publications mentioning bmo-mir-306a

Open access articles that are associated with the species Bombyx mori and mention the gene name mir-306a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 33
The most abundantly expressed miRNAs in B. mori are also highly conserved miRNAs e. g, miR-1, miR-8, miR-10, let-7, miR-263a, miR276a, and miR-306 and were expressed in all four stages, albeit their expression levels vary across different developmental periods. [score:8]
miR-306 is fairly uniformly expressed across the developmental stages, as judged by the number of reads, whereas miR-263 is dynamically regulated from the larva to adult stages. [score:5]
Additionally, we detected two antisense miRNA loci (miR-263-S and miR-263-AS; miR-306-S and miR-306-AS) that are expressed in sense and antisense directions. [score:4]
Interestingly, miR-263 and miR-306 are preferentially and abundantly expressed in pupae and adults, respectively. [score:3]
While miR-116 level steadily increases during progression of larvae to moth (Figure 4d), miR-274b level gradually decreased during the development (Figure 4e), miR-283 and miR-306 levels were relatively uniform in the four stages (Table 3). [score:2]
The same could be true for miR-306 and miR-306-AS. [score:1]
Predicted fold-back of miR-306 (a) and miR-263 (b) from sense (top panel) and antisense strand (bottom panel) and mature miRNA is shown in red. [score:1]
Comparing the mature sequences in each sense and antisense pair showed the seed region in miR-263 and miR263-AS to be almost identical, whereas miR-306 and miR-306-AS showed variations in several internal positions, including the seed region (Figure 6e). [score:1]
miR-306 and miR-263 loci could generate antisense miRNAs in silkworm. [score:1]
Identification of three antisense miRNAs for miR-306, miR-263, and miR-iab-4-AS in silkworm suggests that such antisense miRNAs may be more widespread in other organisms. [score:1]
We recovered eight sequence reads that uniquely mapped to the antisense hairpin sequence of miR-306 (miR-306-AS) and a single read mapping to the antisense hairpin of miR-263 (miR-263-AS) (Figure 6c and 6d), which indicates that the antisense transcripts were processed into mature miRNAs in vivo. [score:1]
Mature sequences of miR-306:miR-306-AS pair (e) and miR-263:miR263-AS (f) aligned for showing identical nucleotide sequences. [score:1]
Analysis of the antisense sequences of miR-306-AS and miR-263-AS loci by mFold showed that they can adopt canonical hairpin secondary structures (Figure 6a and 6b). [score:1]
Sequencing reads uniquely aligned to hairpin sequence of reverse complement of miR-306 (c) and miR-263 (d) followed by their cloning frequency and length (nt). [score:1]
The top three abundant miRNAs of the silkworm are miR-1 (30%), miR-8 (11%) and miR-306 (11%) in the feeding larval stage; miR-1 (40%), miR-8 (18%), and miR-276a (15%) in the spinning larval stage; miR-276a (27%), miR-1 (16%) and miR278 (8%) in the pupal stage; miR-263a (18%), miR276a (16%) and miR-1 (12%) in the adult stage (Additional file 2). [score:1]
Our sequencing efforts identified, for the first time, the prevalence of at least two new miRNA loci, miR-306 and miR-263, that generate miRNAs by convergent transcription from both strands in the silkworm genome. [score:1]
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2
[+] score: 8
For instance, aside from a few commonly expressed miRNAs, such as bmo-miR-8, bmo-miR-13, bmo-miR-263a, bmo-miR-275, bmo-miR-279, bmo-282*, bmo-miR-285 and bmo-miR-306, most of them were found development-related (Table 3) although some may be due to inadequate sampling that often results less reliable statistics. [score:4]
In addition, four highly-conserved miRNAs (bmo-miR-278, bmo-miR-306, bmo-miR-317 and bmo-miR-768) and three less-conserved miRNAs (bmo-miR-1920, bmo-miR-1921 and bmo-miR-1922) were also cloned directly, which were not predicted based on our predicting criteria albeit their canonical precursors (Figure 1). [score:2]
However, several pre-miRNAs were not detected due to the stringency of the filters but discovered with our direct cloning experiments, including bmo-miR-278, bmo-miR-306, bmo-miR-317, bmo-miR-768, bmo-miR-1920, bmo-miR-1921, and bmo-miR-1922. [score:2]
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