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7 publications mentioning eca-mir-155

Open access articles that are associated with the species Equus caballus and mention the gene name mir-155. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 153
The qRT-PCR (Fig 3) analysis confirmed a significant effect of LPS on miR-155 expression (F = 11.9, p = 0.0015), with 1.4-fold upregulation at two hours (p = 0.028) and 1.6-fold upregulation at four hours (p = 0.038) in LPS -treated cells compared to controls. [score:8]
As AKT1 also suppresses miR-155 production, suppression of AKT1 by miR-155 would form a positive feedback loop, enhancing its own expression. [score:7]
Functional analysis of GO terms for TargetScan-predicted miR-155 targets indicated statistical over-representation of the PDGF pathway (5-fold enrichment, p = 0.035) and a range of biological processes (S3 File) including terms such as ‘regulation of cell differentiation’ and ‘positive regulation of signal transduction’, but no terms specifically associated with immune function. [score:7]
By suppressing LPS -induced production of SOCS1 (an inherent negative feedback system), upregulation of miR-155 would be expected to augment the effects of inflammatory cytokines in endotoxemia. [score:6]
MicroRNA-155 was selected due to its significant LPS -induced expression change, whereas miR-146a and miR-146b were selected due to strong time effects, trends towards an LPS effect, high expression and known roles in TLR regulation in other species. [score:6]
The predominant effect of the LPS -induced upregulation of miR-155 on this pathway is therefore difficult to predict, and will depend on the relative efficiency of repression of each target. [score:6]
Suppressor of cytokine signaling-1 is one of the best studied targets of miR-155. [score:5]
TargetScan analysis for miR-155 yielded 262 potential targets with a total context+ score < -0.1. [score:5]
C: Statistical over-representation analysis of Gene Ontology biological function terms for TargetScan miR-155 target predictions. [score:5]
Although statistical enrichment of immune pathways among predicted targets was not demonstrated, bioinformatic predictions indicated a number of possible targets for miR-155 in the toll-like receptor signaling cascade, as well as other aspects of the innate and adaptive immune system (Fig 7). [score:5]
S3 File A: miR-155 target predictions generated by TargetScan. [score:5]
Functional significance of miR-155 upregulation. [score:4]
The LPS -induced upregulation of miR-155 in this mo del is consistent with the majority of human and murine mo dels and cell lines studied to date [7, 43– 46]. [score:4]
Overall, data in other species indicate that the significant upregulation of miR-155 in this mo del is likely to have predominantly pro-inflammatory effects. [score:4]
The differences in production of key LPS -induced cytokines and in miR-155 expression do however indicate that sufficient differential stimulation was achieved to observe an LPS effect. [score:3]
B: Target predictions for eca-miR-155 generated using RNA22, from a list of 51 candidate genes with experimental evidence in other species. [score:3]
The magnitude and time course of expression changes for miR-155 was similar between NGS and PCR analysis (Fig 3). [score:3]
The time course of expression change in miR-155 paralleled that observed for supernatant TNFα concentration. [score:3]
Targeting of other genes by miR-155 is predicted to have anti-inflammatory effects. [score:3]
This is further supported by the temporal association between miR-155 expression changes and production of TNFα, which is induced by NFκB [58]. [score:3]
For example, ectopic expression of miR-155 reverses the effect of dexamethasone on TNFα, IL-6 and nitric oxide production in LPS-stimulated macrophages [66]. [score:3]
This interaction has for example been implicated in the reduction in LPS -induced liver injury and reduced TNFα production observed with a miR-155 inhibitor in a mouse mo del [61]. [score:3]
Interactions between miR-155, its targets, cytokines and transcription factors in innate immune pathways. [score:3]
A number of miRNAs clustered together with miR-155 on the hierarchical clustering analysis (Fig 2), including miRNAs such as miR-146a, miR-146b and miR-132 that had significant increases in expression with time. [score:3]
Functional analysis of equine miR-155 targets. [score:3]
MicroRNA-155 is one of the most studied of all miRNAs, and is implicated in a number of disease processes. [score:3]
Expression changes with time in miR-146a, miR-146b and miR-155. [score:3]
Consistent with the primary hypothesis, differential miRNA expression in response to LPS was demonstrated in equine PBMCs, although only for a single miRNA, miR-155. [score:3]
There is strong evidence that miR-155 -mediated suppression of SOCS1 has biological significance in infection and autoimmunity [60]. [score:3]
After correction for multiple comparisons, only miR-155 showed a statistically significant difference in expression between LPS -treated cells and controls (q-value 0.036 at 4 hours). [score:3]
Predicted targets for miR-155 included 34 genes related to immune responses, of which 26 were associated with innate immunity, and seven with TLR4 signaling. [score:3]
Equine target predictions for miR-155. [score:3]
Regulation of the MIR155 host gene in physiological and pathological processes. [score:2]
Moreover, the miR-155 precursor gene and adjacent regulatory elements examined here have a much closer sequence identity to humans than do the mouse equivalents. [score:2]
For example, miR-155 knockout renders mice more susceptible to infection with Mycobacterium tuberculosis [62]. [score:2]
Organization and conservation of the miR-155 precursor gene. [score:1]
Conservation of the equine MIR155 gene. [score:1]
Pairwise correlations between the cytokines TNFα, IL-10, IFNγ, IL-4 and IL-17, and the -ΔΔCT values for the miRNAs miR-155, miR-146 and miR-146b, are shown in Fig 5. MicroRNA-155 correlated strongly with miR-146a (r = 0.87, p < 0.001) and miR-146b (r = 0.79, p < 0.001), and showed moderate to strong correlations with the cytokines TNFα, IL-10, IFNγ and IL-17 (r = 0.61–0.80). [score:1]
Furthermore, miRNAs of likely functional significance in PBMCs, including miR-146a, miR-146b and miR-155 were previously designated as colon-specific. [score:1]
The encoding gene and flanking sequences for the equine miR-155 precursor were aligned to the human and murine genomes to assess conservation between species. [score:1]
The equine mature miR-155 sequence is identical to the human miRNA, but differs from the murine miRNA by a single nucleotide (C versus U at position 12/23). [score:1]
Conservation of the equine MIR155 geneThe equine mature miR-155 sequence is identical to the human miRNA, but differs from the murine miRNA by a single nucleotide (C versus U at position 12/23). [score:1]
In other contexts, however, the effect of miR-155 on SOCS1 may in fact be protective. [score:1]
This cluster (inset in Fig 2) included miR-155 and seven miRNAs with statistically significant time effects. [score:1]
miR-155 is the principal LPS -induced miRNA in equine PBMCs. [score:1]
Neighbor joining tree to show similarities of human pre-miR-155 to 18 other species. [score:1]
Except for increases in miR-155, there has been remarkably little consensus between studies, likely due in part to differences in the cell populations studies and to methodological differences. [score:1]
The human miR-155 precursor gene on chromosome 21, formerly known as the B-cell Integration Cluster, contains four exons in the most recent genome assembly and Ensembl annotation. [score:1]
The mechanisms of miR-155 induction vary according to cell line and stimulus, but the transcription factors NFκB and AP-1 both play prominent roles in the LPS response [56, 57]. [score:1]
The finding of this study that miR-155 is the principal LPS -induced miRNA in horses, as in other species, supports the hypothesis that the miRNA response is comparable with humans and lends some support to the use of an equine mo del to study this aspect of the response to endotoxin. [score:1]
In such cases, miR-155 could exacerbate the clinical syndrome. [score:1]
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2
[+] score: 31
This, in turn, suggests that HMB may play an important role in the inflammation processes as an anti-inflammatory factor which could be related to the inhibition of pro-inflammatory cytokine secretion by HMB -induced miR-146 over -expression and activate innate immunity response by miR-155 over -expression. [score:7]
The following cell proliferation-related genes were identified: jak2 (target of identified miR-101, miR-155), rarg (miR-142-3p, miR-30c), pten (miR-146a, miR-374b, miR-193a), ets1 (miR-221/222), and rarb (miR-146a, miR-146b); cell differentiation-related target genes: jak2 (miR-155), pten (miR-1), klf4 (miR-1, miR-146a, miR-206) and ets1 (miR-221/222). [score:5]
Taken together, changes in expression of pro-proliferative (miR-133a/b, miR-146a/b, miR-222/221) and differentiation-related miRNAs (miR-1, miR-133a/b, miR-155, miR-193a, miR-204, miR-206, miR-221/222, miR-331, miR-324, miR-374, miR-675) were observed following HMB incubation and exposition of ESC cultures to H [2]O [2], with concomitant changes in expression of their corresponding DET. [score:5]
They were represented by miR-204 which was upregulated in differentiated human cardiomyocyte progenitor cells [32] and miR-155 (↑) and miR-193a (↑), known to regulate cell differentiation in muscle cells [33] and brown fat cells [34], respectively. [score:5]
Moreover, we identified several target genes which are involved in muscle organ development: sgcd (miR-142-3p), scd (miR-1, miR-128), cav3 (miR-101), tcf12 (miR-101, miR-142-3p, miR-155, miR-204, miR-208, miR-221/222), and col19a1 (miR-1, miR-206), as modulated in ESC treated with HMB. [score:4]
Moreover, miR-155, known to be an immunomodulatory miRNA, acts as a broad limiter of pro-inflammatory gene expression in muscle [47] possessing the same trend which was observed in our experiment in the case of HMB -treated group. [score:3]
Toll-like receptor pathway is related to the inflammatory activity [61] and different kind of myopathies and could be modulated by miR-155 [62]. [score:1]
RIG-I-like receptor pathway is known to play a crucial role in innate response [63] which is necessary to activate early muscle regeneration and may be modulated by three identified miRNAs: miR-146a, miR-146b, and miR-155. [score:1]
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3
[+] score: 21
Alone or in combination with SMAD4, miR-224 could be an independent prognostic marker for the survival of CRC patients [62, 63 MVc contains miR-155, an oncogenic microRNA that is significantly up-regulated by coculture with LC-MSCs and by S100A4 ectopic overexpression [39] (Table 1). [score:6]
We have revealed common small non-coding RNA molecules (miR-26a, miR-195, miR- miR-126, miR-122, miR-21, miR-155, miR-9, miR-135b, miR-29b, miR-142-3p, miR-210, miR-181, miR- 224) in HCC and CRC, which suppress the expression of multiple genes involved in tumor- stromal interactions, immune invasion and tumor angiogenesis. [score:5]
For instance, miR-210 and miR-155, which are overexpressed in HCC and CRC, promote the metastatic potential of HCC cells by targeting the vacuole membrane protein 1 (VMP1), in this way being an example of tumor- to- stroma communication (Figure 1) [39, 50, 51, 52]. [score:5]
On the one hand, the intravenous administration of B-cell derived exosomes loaded with miR-155 correlated with significantly increased miR-155 expression in the liver of miR -knockout mice compared with controls [1, 57, 59]. [score:3]
Equally, miR-155 has a pivotal role in modulating liver-cancer -associated mesenchymal stem cell (LC-MSCs), the invasion promoting effect being attenuated by a miR-155 antagonist [39]. [score:1]
An example of tumor- stroma communication is provided by theS100A4-miR155-SOCS1-MMP9 axis. [score:1]
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4
[+] score: 4
Whether hsa-miR-155 also plays a role in the immune control of polyomaviruses and more specifically in polyomavirus -induced diseases that are dependent on changes in the host immune system is of particular interest but remains to be determined. [score:3]
Recent work has also identified hsa-miR-155 as an important host cell miRNA involved in a more general role in the immune response upon viral infection. [score:1]
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5
[+] score: 2
Specifically, miR-155 plays a major role in both allergy and anti-parasitic immunity [27]. [score:1]
Okoye I. S. Czieso S. Ktistaki E. Roderick K. Coomes S. M. Pelly V. S. Kannan Y. Perez-Lloret J. Zhao J. L. Baltimore D. Transcriptomics identified a critical role for Th2 cell-intrinsic miR-155 in mediating allergy and antihelminth immunityProc. [score:1]
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6
[+] score: 2
Other miRNAs have also been implicated in muscle development, including miR-26a (23), miR-27b (24), miR-29 (25), miR- 125b (26), miR-155 (27), miR-128a (28) and miR- 181 (29). [score:2]
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7
[+] score: 1
Other miRNAs from this paper: hsa-mir-17, hsa-mir-28, hsa-mir-223, hsa-mir-127, hsa-mir-188, hsa-mir-194-1, hsa-mir-155, hsa-mir-194-2, hsa-mir-30e, hsa-mir-362, hsa-mir-363, hsa-mir-367, hsa-mir-379, hsa-mir-196b, hsa-mir-450a-1, hsa-mir-431, ssc-mir-28, hsa-mir-493, hsa-mir-512-1, hsa-mir-512-2, hsa-mir-500a, hsa-mir-501, hsa-mir-502, hsa-mir-450a-2, hsa-mir-513a-1, hsa-mir-513a-2, hsa-mir-506, hsa-mir-508, hsa-mir-509-1, hsa-mir-532, hsa-mir-615, hsa-mir-660, bta-mir-127, bta-mir-30e, bta-mir-17, bta-mir-450a-2, bta-mir-532, bta-mir-363, bta-mir-660, hsa-mir-891a, hsa-mir-892a, hsa-mir-509-2, hsa-mir-450b, hsa-mir-892b, hsa-mir-708, hsa-mir-509-3, hsa-mir-1285-1, hsa-mir-1285-2, hsa-mir-1248, ssc-mir-17, bta-mir-155, bta-mir-188, bta-mir-194-2, bta-mir-196b, bta-mir-223, bta-mir-28, bta-mir-362, bta-mir-367, bta-mir-379, bta-mir-431, bta-mir-493, bta-mir-500, bta-mir-502a-1, bta-mir-502a-2, bta-mir-502b, bta-mir-615, bta-mir-708, bta-mir-1248-1, bta-mir-1248-2, ssc-mir-450a, bta-mir-2320, bta-mir-1388, bta-mir-194-1, bta-mir-450a-1, eca-mir-30e, eca-mir-367, eca-mir-684, eca-mir-196b, eca-mir-615, eca-mir-708, eca-mir-194-1, eca-mir-493a, eca-mir-17, eca-mir-1248, eca-mir-28, eca-mir-127, eca-mir-379, eca-mir-431, eca-mir-493b, eca-mir-194-2, eca-mir-188, eca-mir-223, eca-mir-362, eca-mir-363, eca-mir-450a, eca-mir-450b, eca-mir-450c, eca-mir-500-1, eca-mir-500-2, eca-mir-501, eca-mir-502, eca-mir-508, eca-mir-509a, eca-mir-532, eca-mir-660, ssc-mir-30e, ssc-mir-196b-1, ssc-mir-450b, ssc-mir-127, ssc-mir-532, ssc-mir-708, ssc-mir-1285, ssc-mir-500, hsa-mir-514b, ssc-mir-363-1, ssc-mir-450c, hsa-mir-500b, ssc-mir-194b, ssc-mir-155, ssc-mir-362, bta-mir-3601, ssc-mir-615, ssc-mir-2320, bta-mir-450b, ssc-mir-194a, ssc-mir-196b-2, ssc-mir-363-2, ssc-mir-493, hsa-mir-892c, eca-mir-1388, eca-mir-514b, eca-mir-506a, eca-mir-509b, bta-mir-194b, ssc-mir-1388, ssc-mir-223, ssc-mir-660, bta-mir-194b-2, bta-mir-1949
Sometimes these duplicated annotations were found twice on the same chromosome in close proximity (for example mir-196b twice on the same strand, and both mir-615 and mir-194 twice, once on each strand) and sometimes they were found both on the assembled chromosomes and within the unplaced contigs (for example mir-127, mir-155). [score:1]
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