sort by

7 publications mentioning ssc-mir-365-1

Open access articles that are associated with the species Sus scrofa and mention the gene name mir-365-1. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 66
Furthermore, our study found that the expression of miR-365-3p was significantly upregulated from Day 15 of gestation to Days 26, 50, and 95 of gestation, which shows similar expression pattern with the PLET1-L transcript as shown in Figure 5. This is opposed to the observations that miR-365-3p could reduce the PLET1-L 3′ UTR luciferase reporter activity in vitro and that the PLET1-L 3′ UTR showed higher luciferase activity. [score:8]
The quantitative RT-PCR (qRT-PCR) was carried out to detect the expression levels of the two PLET1 transcripts and the miR-365-3p expressed in the conceptuses (Day 15 of gestation) and placentas (chorioallantoic tissue, Days 26, 50, and 95 of gestation) on the CFX384 real-time PCR detection System (Bio-Rad Laboratories, Inc. [score:5]
The value of 2−ΔΔ Ct represents the relative expression level of miR-365-3p. [score:3]
To detect the expression profile of the miR-365-3p, total RNA was reverse transcribed using Mir-X [TM] miRNA First-Strand Synthesis Kit (Takara Bio Inc. [score:3]
Moreover, the present data suggest that miR-365-3p has an impact on the expression of the PLET1 gene. [score:3]
2.6. miR-365-3p Was Differentially Expressed during Days 15, 26, 50, and 95 of Gestation in Pigs. [score:3]
In this study, the expression data of miR-365-3p and PLET1-L 3′ UTR were derived from porcine placenta samples. [score:3]
In addition, overexpression of the miR-365-3p mutant had no significant effect on the PLET1-S 3′ UTR luciferase reporter activity. [score:3]
The results suggest that a possible interaction may exist between PLET1-S 3′ UTR and miR-365-3p, although we did not find the perfect seed matches of miR-365-3p in the PLET1-S 3′ UTR by using the online miRNA target prediction tools described above. [score:3]
The qRT-PCR was used to detect the expression pattern of miR-365-3p in conceptus and placenta during the periods Days 15, 26, 50, and 95 of gestation. [score:3]
However, the cell location of miR-365-3p has yet to be assessed, so data shown in Figure 8 may not exactly be the expression pattern of miR-365-3p in trophoblast cells. [score:3]
This may explain the unexpected correlation of expression pattern between miR-365-3p and the PLET1-L transcript in porcine placenta. [score:3]
We found that overexpression of the miR-365-3p significantly decreased the luciferase activity of the wild-type plasmid compared to that of the NC control. [score:2]
As shown in Figure 8, the miR-365-3p expression levels were significantly increased in placentas from Days 26, 50, and 95 of gestation as compared to that in conceptuses from Day 15 of gestation. [score:2]
As expected, overexpression of the miR-365-3p mutant had no significant effect on luciferase activity of this reporter compared to that in cells transfected with NC-miRNA. [score:2]
Therefore, further studies using porcine trophoblast cells will be needed to elucidate the detailed molecular mechanisms underlying the miR-365-3p -mediated regulation on the porcine PLET1. [score:2]
It is worth noting that the 5′ end of the seed sequence of miR-365-3p matches the first 4 bp of the distal (second) core polyadenylation signal (AUUAAA) (Figure 4); Third, we validated the interaction between miR-365-3p and porcine PLET1 by transfection of three constructs (the PLET1-L 3′ UTR, PLET1-S 3′ UTR and mutant PLET1-L 3′ UTR where the putative miR-365-3p binding site was mutated) into PK15 cells, respectively. [score:1]
Together with data shown in Figure 7, our study demonstrated the interaction between the PLET1 and miR-365-3p in vitro, and indicates that in addition to the predicted canonical binding site in the PLET1-L 3′ UTR, a non-canonical miR-365-3p binding site might be within the PLET1-S 3′ UTR. [score:1]
Validation of the Interaction between miR-365-3p and PLET1. [score:1]
We then developed a second PLET1-L 3′ UTR luciferase reporter carrying a mutant miR-365-3p binding site. [score:1]
The miRNA mimics of miR-365-3p, the miR-365-3p mutant (mut-miR-365-3p), and NC-miRNA (a scrambled sequence) were each synthesized as duplexes. [score:1]
As showed in Figure 7B, neither miR-365-3p nor miR-365-3p mutants could result in significant decrease in the PLET1-L-mut luciferase reporter activity (Figure 7B). [score:1]
Finally, we constructed the truncated wild-type plasmid containing the predicted binding site of miR-365-3p and the corresponding mutant plasmid containing the mutated seed binding site. [score:1]
These findings indicate the existence of the interaction between miR-365-3p and the predicted binding site in the porcine PLET1-L 3′ UTR. [score:1]
Random 4 base substitutions in the seed sequence of miR-365-3p were introduced to create the miR-365-3p mutant (mut-miR-365-3p) when synthesized, and NC-miRNA was used as the negative control. [score:1]
We found the following: (1) The PLET1-S 3′ UTR luciferase reporter activity decreased ~2.0-fold in cells transfected with miR-365-3p compared to NC-miRNA, and overexpression of miR-365-3p mutant did not result in the decrease of PLET1-S 3′ UTR luciferase activity (Figure 7A); (2) The PLET1-L 3′ UTR luciferase reporter activity decreased ~3.0-fold in cells transfected with miR-365-3p compared to NC-miRNA. [score:1]
Therefore, our observation may suggest a non-canonical binding mechanism involved in the interaction between miR-365-3p and the PLET1-S 3′ UTR. [score:1]
Our study predicted a putative binding site of miR-365-3p in pig PLET1-L 3′ UTR. [score:1]
Of the four miRNAs, only the miR-365-3p mimic could result in decrease in luciferase activity. [score:1]
The two plasmids (PLET1-S and PLET1-L) that mentioned above and PLET1-L 3′ UTR plasmid where the putative miR-365-3p binding site was mutated (designed as PLET1-L-mut) were used to validate the interaction of miR-365-3p and PLET1-L 3′ UTR. [score:1]
In summary, these results demonstrated the interaction between the PLET1 and miR-365-3p in vitro. [score:1]
[1 to 20 of 31 sentences]
2
[+] score: 15
Five upregulated (miR-146a-5p, miR-146b, miR-365-3p, miR-92a, and miR-181c) and five downregulated (miR-30b-3p, miR-214, miR-140-3p, miR-664-3p, and miR-1307) miRNAs from the microarray were selected randomly to conduct real-time PCR. [score:7]
Target prediction of the 29 differentially expressed miRNAs revealed that miR-148a-3p, miR-27b-3p, miR-423-5p, miR-125a, miR-181c, and miR-365-3p target TNF-α. [score:7]
Among them, five miRNAs (miR-365-3p, miR-378, miR-30c-5p, miR-181a, and miR-92a) had two copies in the genome, while P-m0299-5p did not map to any chromosome in the database available (supplemental Table S3). [score:1]
[1 to 20 of 3 sentences]
3
[+] score: 10
For instance, Y-82 targets ADCY6 that is involved in cAMP signalling; miR-4334-5p, Y-67, and Y-12 potentially target ITPR3 of IP3 signalling; ssc-miR-365-3p potentially target the GH-secretion-related molecule SNAP23. [score:7]
In addition, there were other miRNAs such as miR-365, miR-183, miR-149, miR-224, and miR-199b that were frequently aberrantly expressed in GH-secreting or other pituitary adenomas[45, 46], although their detailed function is still unknown. [score:3]
[1 to 20 of 2 sentences]
4
[+] score: 10
We compared the basal expression levels of miR-125b, miR-155, miR-23a and miR-365 in PAMs by qPCR and found that miR-125b was among the most highly expressed miRNAs examined (data not shown). [score:4]
To screen the potential miRNAs which can reduce PRRSV replication, the mimics or inhibitors of 10 miRNAs (Table S1), including miR-24, miR-93, miR-122, miR-125b, miR-146a, miR-155, miR-181, miR-196, miR-351, and miR-365, were chosen and synthetized. [score:3]
The mimics and inhibitors of miR-24, miR-93, miR-122, miR-125b, miR-146a, miR-155, miR-181, miR-196, miR-351, and miR-365 (shown in Table S1) were obtained from GenePharma (Shanghai, China). [score:3]
[1 to 20 of 3 sentences]
5
[+] score: 7
miR-365-3p was found to have an effect on the expression of the placenta-expressed transcript 1 (PLET1) gene, which is important for placental development in pigs [44]. [score:6]
The other two miRNAs (miR-365-3p and miR-4334-3p) are less studied. [score:1]
[1 to 20 of 2 sentences]
6
[+] score: 7
As shown in the heat map, compared with sample on PID 0, most miRNAs were down-regulated in sample on PID 4, whereas only few miRNAs were differentially expressed in sample on PID 7. Compared with sample on PID 4, a majority of miRNAs were up-regulated in sample on PID 7. To validate the Solexa sequencing results, qRT-PCR was performed separately to investigate the relative expression levels of 5 randomly selected DE miRNAs (ssc-miR-424-3p, ssc-miR-542-5p, ssc-miR-365-5p, ssc-miR-450b-5p, ssc-miR-450a). [score:7]
[1 to 20 of 1 sentences]
7
[+] score: 5
Interestingly, we found that three miRNAs (miR-363, miR-365 and miR-422b) were differentially expressed between E33 and Adu, despite their expression not being significantly different when comparing either E33 to E65 or E65 to Adu. [score:5]
[1 to 20 of 1 sentences]