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4 publications mentioning sja-mir-8

Open access articles that are associated with the species Schistosoma japonicum and mention the gene name mir-8. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 16
Similar results were also observed in cells transfected with miR-8, miR-3479 and miR-1989 mimics and the corresponding recombinant plasmids expressing schistosome mRNA target sites (Fig 3). [score:5]
Among the validated miRNA targets, we found that male enriched miR-8 and miR-3479 regulate the molecules likely involved in the Wnt (GenBank Accession Nos: FN313640 and DQ643829.2) and TGF-β (Accession No. [score:4]
Transfection of miR-2 (A), Let-7a (B), bantam (C), miR-8 (D and E), miR-31 (F), miR-1989 (G), and miR-3479 mimics (H) into HEK293T or Hela cells led to a significant reduction of luciferase activity from co -transfected plasmids containing their corresponding target regions. [score:3]
1005423.g003 Fig 3Transfection of miR-2 (A), Let-7a (B), bantam (C), miR-8 (D and E), miR-31 (F), miR-1989 (G), and miR-3479 mimics (H) into HEK293T or Hela cells led to a significant reduction of luciferase activity from co -transfected plasmids containing their corresponding target regions. [score:3]
Hela or HEK293T cells were transfected with these recombinant plasmids, control plasmids (pGL3), and the corresponding 2`-O-methyl and phosphorothioate miRNA mimics representing bantam, miR-8, miR-31, miR-1989, miR-3479, or control mimics (scrambled or mismatched seeding region of these miRNA) (S8 Table). [score:1]
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2
[+] score: 10
The result showed that some miRNA genes, including miRNA-2e-5p, miRNA-2e-3p, miR-71b, miR-2a, miR-2f, miR-124 and miR-31, tended to be enriched in immature worms, whereas miR-125, miR-8 and bantam exhibited a higher abundance in mature adults (Figure 3D), suggesting that different miRNAs could play a distinct role in different development stages. [score:2]
The tenth nucleotide uracil (U) of Schistosoma miR-8 is significantly distinct from cytidine (C) of all orthologs or their paralogs, including S. mediterranea miR-8. miR-8 of Platyhelminthes (Schistosoma and S. mediterranea) and Arthropoda share the same eleventh adenine (A) with Nematoda miR-236, which is different from U or guanine (G) of the same position of Urochordata and Vertebrata miR-141, miR-200 and miR-429 (Figure 5B). [score:1]
Interestingly, Schistosoma miR-8, shared by both schistosomes, was homologous with Arthropoda orthologs, as well as Nematoda miR-236, Urochordata and Vertebrata miR-141, miR-200 and miR-429 (Figure 5B). [score:1]
Some miRNA genes, including miRNA-2e-5p, miRNA-2e-3p, miR-71b, miR-2a, miR-2f, miR-124 and miR-31, seem to be preferentially enriched in immature worms, whereas miR-125, miR-8 and bantam exhibited a higher abundance in mature adults (Figure 3D). [score:1]
Phylogenetic analysis implying that Schistosoma miR-8, which exhibited a different clade from the S. mediterranea ortholog, could represent an ancient prototype of Arthropoda orthologs, and share the ancestral miRNA gene origin with Urochordata and Vertebrata miR-141 and miR-200a (Figure S1). [score:1]
Although 21 S. japonicum miRNAs are orthologs of known miRNAs within the metazoans, some nucleotides at many positions of Schistosoma miRNAs, such as miR-8, let-7, miR-10, miR-31, miR-92, miR-124, and miR-125, are indeed significantly distinct from other bilaterian orthologs. [score:1]
Phylogenetic analysis was performed for the known miR-8 and its orthologs or paralogs from bilaterian animals using MEGA 4 software. [score:1]
Figure S1Phylogenetic analysis of Schistosoma miR-8 and its orthologs or paralogs. [score:1]
Some of the common Schistosoma miRNAs, including let-7, miR-8, miR-10, miR-31, miR-92, miR-124 and miR-125, are evolutionally conserved across bilaterian animals (Figure 5A). [score:1]
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3
[+] score: 1
Only one miRNA, the previously reported sja-miR-8-5p [19], was not detected in this study (Table S6). [score:1]
[1 to 20 of 1 sentences]
4
[+] score: 1
Membranes were incubated with biotin-labeled probes complementary to candidate miRNA sequences (sja-miR-1a, sja-miR-1b, sja-miR-7, sja-miR-71b, sja-miR-71a, sja-miR-87, sja-miR-124, sja-miR-281, sja-miR-2b, sja-miR-2a, sja-miR-307, sja-miR-31, sja-miR-133, sja-miR-310, sja-miR-8, sja-miR-10, sja-miR-36, sja-miR-61, sja-miR-277, sja-miR-219, sja-miR-190, sja-miR-candidate-03). [score:1]
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