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8 publications mentioning hsa-mir-4284

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-4284. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 86
The anti-cancer effects of BBMD3 in these cells was shown to be associated with the up-regulation of miR-4284 expression and activation of the stress-response JNK signaling pathway, as well as the AP-1 transcriptional factor, a JNK downstream target. [score:8]
After these GBM neurospheres were transfected with, and allowed to express for 24 hours, the miR-27a and miR-4284 anti-sense inhibitors, or the control miRNA inhibitor sequence, cells were incubated with 5 µM BBMD3 for another 24 hours, at which point cell viability was determined. [score:7]
In our study, we found that BBMD3 treatment up-regulated the expression of miR-4284 more than four-fold in GBM stem-like cells. [score:6]
Therefore, BBMD3 mediated inhibition of GBM stem-like cell viability depends, at least in part, on increasing expression of miR-4284. [score:5]
Thus, BBMD3 inhibits cell viability and induces apoptosis in these stem-like cells, at least in part, through increasing expression of miR-4284. [score:5]
0094443.g005 Figure 5(A) Cells from PBT003 and PBT030 neurospheres were transfected with 60 nM of anti-sense inhibitors against miR-4284, miR-27a and an unrelated control miRNA, and the inhibitor was allowed to interact with the miRNA for 48 hours prior to determining the viability of the cells. [score:5]
However, transfection of the miR-27a anti-sense inhibitor into these neurosphere cultures was not as effective at reducing the cytotoxic effect of BBMD3 treatment on the GBM stem-like cells as transfection with the miR4284 inhibitor. [score:5]
The results shown in Figure 5B indicated that, in contrast to the control miRNA inhibitor, cells transfected with the anti-sense miR-4284 inhibitor showed greater viability (two fold) following BBMD3 treatment in both the PBT008 and PBT030 neurosphere cultures. [score:5]
We also show that cell killing is associated with up-regulation of miRNA-4284 and the JNK/AP-1 signaling pathway. [score:4]
In contrast, the two most up-regulated miRNAs are miR-4284 and miR-27a; increasing 4.48 and 2.25 fold respectively. [score:4]
miR-4284 Inhibitor Blocks the Effects of BBMD3 on the Viability of GBM Neurospheres. [score:3]
The results shown in Fig. 5A demonstrated that miR-27a and miR-4284 inhibitors alone did not effectively decrease cell viability relative to that of the untransfected cells (untran). [score:3]
60 nM of human anti-sense miRNA inhibitors (i. e., synthetic oligonucleotides) against hsa-miRNA-4284 and hsa-miRNA-27a were transfected into cells derived from PBT008 and PBT030 neurospheres. [score:3]
Five hours later, 60 nM miR-4284 or miR-27a anti-sense inhibitors were transfected into the cells by RNAiFect transfection reagent. [score:3]
Human miRNA inhibitors (synthetic oligonucleotides) against hsa-miRNA-4284 and has-miRNA-27a were purchased from GeneCopoeia. [score:3]
The effect of miR-4284 and miR27a inhibitors on the cytotoxic activity of BBMD3 in PBT003 and PBT030 neurospheres. [score:3]
BBMD3 Increases the Expression of miR-4284 and miR-27a in PBT003, PBT008, PBT022 and PBT030 Neurospheres. [score:3]
An anti-sense inhibitor of miR-4284 activity could partially block the anticancer effects of BBMD3 on those GBM stem-like cells. [score:3]
As an aside, it should be noted that all of the functions attributed to miR-4284 expression have not yet been identified. [score:3]
We then tested the effect of miR-27a and miR-4284 inhibitors on the cytotoxic activity of BBMD3 using PBT008 and PBT030 neurospheres. [score:3]
To date, we have been unable to find any publication describing the regulatory function(s) of miR-4284. [score:2]
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2
[+] score: 47
Among the 77 miRNAs, 7 miRNAs, hsa-miR-33b-3p, hsa-miR-4284, hsa-miR-663a, hsa-miR-150-5p, hsa-miR-1233-3p, hsa-miR-671-3p, and hsa-miR-140-3p, were selected for further validation based on the fact that they were in the top 20 up- or downregulated miRNAs as found in microarray microRNA expression profiling presented in Fig.   1a, b. More specifically, miR-33b-3p and miR-4284 were among the top upregulated, and miR-663a, miR150-5p, miR-1233-3p, miR-671-3p, and miR-140-3p were downregulated. [score:12]
The expression levels of hsa-miR-33b-3p and hsa-miR-4284 coincided between the two methodologies, while hsa-miR-1233-3p, hsa-miR-140-3p, hsa-miR-150-5p, hsa-miR-663a, and hsa-miR-671-3p were marginally overexpressed in microarray analysis and appeared to be down-regulated with qRT-PCR Moreover, we found that hsa-miR-33b-3p and hsa-miR-4284 expression levels coincided between microarray analysis and qRT-PCR, exhibiting decreased expression in serum of OA samples compared to controls. [score:11]
The expression levels of hsa-miR-33b-3p and hsa-miR-4284 coincided between the two methodologies, while hsa-miR-1233-3p, hsa-miR-140-3p, hsa-miR-150-5p, hsa-miR-663a, and hsa-miR-671-3p were marginally overexpressed in microarray analysis and appeared to be down-regulated with qRT-PCR Moreover, we found that hsa-miR-33b-3p and hsa-miR-4284 expression levels coincided between microarray analysis and qRT-PCR, exhibiting decreased expression in serum of OA samples compared to controls. [score:11]
We, next, validated with qRT-PCR, which offers high accuracy, sensitivity, and dynamic range [46] the expression levels of 7 selected out of the 77 DE miRNAs, which were among the top 20 up- or downregulated in the microarray screening, namely, hsa-miR-33b-3p, hsa-miR-4284, hsa-miR-663a, hsa-miR-150-5p, hsa-miR-1233-3p, hsa-miR-140-3p, and hsa-miR-671-3p, in serum and in OA and healthy articular cartilage samples. [score:6]
Hsa-miR-1233-3p (a), hsa-miR-140-3p (b), hsa-miR-150-5p (c), hsa-miR-33-3p (d), hsa-miR-4284 (e), hsa-miR-663a (f), and hsa-miR-671-3p (g) manifested an area under the curve (AUC) value > 0.8 (AUC > 0.8) and p < 0.01 We verified by qRT-PCR the expression levels of the seven selected miRNAs (hsa-miR-33b-3p, hsa-miR-4284, hsa-miR-663a, hsa-miR-150-5p, hsa-miR-1233-3p, hsa-miR-140-3p, and hsa-miR-671-3p) in all serum samples. [score:3]
No significant differences were observed for hsa-miR-33-3p, hsa-miR-4284, hsa-miR-663a, and hsa-miR-1233-3p expression levels between OA and healthy articular cartilage. [score:3]
The expression levels of 7 selected miRNAs screened with miRNA microarrays, as mature hsa-miR-33b-3p, hsa-miR-4284, hsa-miR-663a, hsa-miR-150-5p, hsa-miR-1233-3p, hsa-miR-140-3p, and hsa-miR-671-3p, were evaluated in serum samples from 12 OA patients and 12 healthy controls. [score:1]
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3
[+] score: 20
The analysis performed on PDX mo del showed a deregulation of only five microRNAs, two up-regulated (miR-4284 and miR-375) and three down- (miR-22-3p, let-7c, and miR-214-3p). [score:5]
Its role is not clear, but in a work of Yang, miR-4284 seems to be overexpressed by bermamine, a natural chemotherapeutic agent, in glioblastoma cells; they demonstrated its role as tumor suppressor, promoting apoptosis in cancer stem cells of glioblastoma [53]. [score:5]
The unique common microRNA up-regulated in both mo dels is miR-4284. [score:4]
Further, we revealed that miR-4284 was up-regulated in both in vitro and in vivo mo dels after trabectedin treatment. [score:4]
Further, a validation of the expression of miR-4284, miR 21-5p, and miR-494-3p was performed using TaqMan Assay. [score:2]
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4
[+] score: 10
We validated by RT-qPCR analysis the down-regulation of Let-7i-5p and miR-199a-3p and the up-regulation of miR-4284 during the conversion of white to brite adipocytes (Fig. 1C). [score:7]
For example, miR-4284, identified only in humans and not yet associated to any function in adipocytes, have as predicted targets in silico the well-known obesity associated gene FTO. [score:3]
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5
[+] score: 9
CXCL5, which has been reported to function for various cancer development, has been confirmed to be a direct target of miR-4284 [20], suggesting that CXCL5 might be also involved in BBMD3′s effect. [score:5]
The up-regulation of miR-4284 can partially accounts for antitumor mechanism of BBMD3, although the relevant signaling cascade is not completely clear [19]. [score:4]
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6
[+] score: 7
logFC p-value B-statistic microRNA 0.98 3.53E-08 9.07 hsa-miR-1244 0.74 2.34E-07 7.34 hsa-miR-494 0.45 6.83E-07 6.34 hsa-miR-1979 0.31 3.97E-06 4.66 hsa-miR-1826 0.77 9.14E-06 3.85 hsa-miR-1281 −0.31 1.13E-05 3.65 hsa-miR-202 −0.37 1.14E-05 3.64 hsa-miR-4284 −0.49 1.70E-05 3.25 hsa-miR-221-star −0.89 6.51E-05 1.94 hsa-miR-3172 −0.63 9.48E-05 1.57 hsa-miR-548a-3p −0.43 1. 02E-04 1.49 hsa-miR-1272 −0.34 1.29E-04 1.27 hsa-miR-15a −0.40 1.59E-04 1.06 hsa-miR-3152 −0.22 2.56E-04 0.59 hsa-miR-142–5p 0.24 3.03E-04 0.43 hsa-miR-4270 −0.29 3.27E-04 0.35 hsa-miR-1910 −0.42 3.29E-04 0.35 hsa-miR-34a-star −0.18 3.78E-04 0.21 hsa-miR-381 −0.30 4.10E-04 0.13 hsa-miR-450b-5p 0.30 4.34E-04 0.08 hsa-miR-1469 We also looked for differentially expressed miRNAs (BH adjusted p < 0.05) that may target differentially expressed mRNAs (BH adjusted p < 0.05). [score:7]
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7
[+] score: 3
Eleven miRNAs (miRNA-4530, miRNA-4739, miRNA-762, miRNA-4787-5p, miRNA-940, miRNA-3676-5p, miRNA-6090, miRNA-150-5p, miRNA-4516, miRNA-4284, miRNA-3656) demonstrated a similar expression trend in both the acute and chronic groups. [score:3]
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8
[+] score: 2
Seven human miRNAs (hsa-miR-1291, hsa-miR-1246, hsa-miR-1248, hsa-miR-1274b, hsa-miR-1973, hsa-miR-4284 and hsa-miR-3656) could be accurately mapped to other ncRNAs, which included snoRNAs, snRNAs, tRNAs and rRNAs (Table 1). [score:1]
Similarly, those miRNAs involved in cross-mapping events, such as hsa-miR-1246 and hsa-miR-4284 in Table 1 (their pre-miRNAs were classified as pseudo miRNA precursors according to the miPred web server), could also be miRNA -mimics that may be by-products of other ncRNAs. [score:1]
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