3 publications mentioning mmu-mir-5107

Open access articles that are associated with the species Mus musculus and mention the gene name mir-5107. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

The miRNA that had the highest number of predicted targets was mmu-miR-5107 (212 targets) and the best matched target was Pafah2 (platelet-activating factor acetylhydrolase 2, NM_133880).

The mmu-miR-5107 was expressed marginally higher in the 21d sample (1.19 ± 0.12) than in the 14d (1 ± 0.14), while the expression level of mmu-miR-novel-1 was slightly lower in the 21d sample (0.63 ± 0.07) than that in the 14d RNA (1 ± 0.11).

Six representative miRNAs, including mmu-mir-5107 and mmu-miR-novel-1 (common to both time points post-infection); 14df-m0014-5p and 14df-m0027-3p (14d-specific miRNAs), and 21df-m0027-5p and 21df-m0013-5p (21d-specific miRNAs), which had higher copy numbers than others, were chosen and used in a quantitative PCR analysis (Figure  3).

2

The upregulated miRNAs included mmu-miR-34a-5p, mmu-miR-129b-5p, mmu-miR-451a, mmu-miR-144-5p and mmu-miR-129b-3p, whereas highly downregulated miRNAs included mmu-miR-100-5p, mmu-miR-99a-5p, mmu-miR-33-5p, mmu-miR-125a-5p, mmu-miR-128-1-5p, mmu-miR-181b-1-3p, mmu-miR-188-5p, mmu-miR-196b-5p, mmu-miR-211-5p, mmu-miR-224-5p, mmu-miR-455-3p, mmu-miR-504-5p, mmu-miR-592-5p, mmu-miR-5107-3p, mmu-miR-5120, and mmu-let-7i-3p.

3

miRNA Fold change at 3 dpi Fold change at 5 dpi mmu-miR-466h-3p NS (Not significant) 14.311053 mmu-miR-346-5p NS 3.4766614 mmu-miR-877-3p NS 3.416667 mmu-miR-7a-5p NS 2.1413074 mmu-miR-5107-5p NS −2.047792 mmu-miR-3473a −2.2872427 −2.1317267 mmu-miR-150-5p NS −2.1770155 mmu-miR-3473b −3.2475147 −2.282881 mmu-miR-721 NS −2.6864858 mmu-miR-669b-5p NS −2.9408455 mmu-miR-709 NS −3.0065749 mmu-miR-669n NS −3.0094464 mmu-miR-468-3p NS −3.40051 mmu-miR-466m-5p NS −4.33538 mmu-miR-32-3p NS −4.5324426 mmu-miR-466h-5p NS −4.9673104 mmu-miR-3082-5p NS −6.01648 mmu-miR-466i-5p NS −7.6776285 mmu-miR-1187 NS −8.772696 mmu-miR-574-5p NS −9.259378 To confirm the validity of the differentially expressed miRNAs that had been identified by microarray analysis, we performed real-time PCR on all 20 of these miRNAs using the polyA tailing technique.

Among them, miR-5107-5p could regulate the MAPK p38 signaling pathway via the activation of MAPKAPK2 (MAPK activated protein kinase 2/MK2) in particularly (Figure 5 and Supporting File 3), thereby promoting the production of microglial-derived cytokines like TNF-α, IL-6, or IFN-γ, inside the BBB (Kaminska et al., 2009; Wang et al., 2015).