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2 publications mentioning ola-let-7a-2

Open access articles that are associated with the species Oryzias latipes and mention the gene name let-7a-2. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 57
Recently, let-7, miR-122 and miR-143 have been shown to have tumor suppressive activity, and de-regulated let-7 expression has been associated with cancer [14], [16], [41]. [score:6]
All let-7 members share a similar seed region which regulates the interaction between miRNA and its target genes, so it is generally believed that let-7 family imposes similar biological functions among different species. [score:4]
Moreover, let-7 transgenic mice study indicated an impairment of glucose tolerance through the deregulation of insulin receptor (Insr) and insulin receptor substrate 2 (Irs2) in liver [34], suggesting that the hypoxia -mediated let-7a suppression may result in the dysfunction of medaka liver under hypoxia. [score:4]
Our data also demonstrated the suppression of let-7a in liver and brain tissues under hypoxic condition. [score:3]
MiRNAs that were highly expressed in both sexes or that were tissue-enriched were chosen: let-7a (non-tissue enriched), miR-9-3p (brain-enriched), miR-122 (liver-enriched), miR-27a (ovary-enriched), and miR-2184 (testis-enriched). [score:3]
Consistent with our RNA sequencing data, qRT-PCR analysis (Figure S2) showed that let-7a was amplified in all of the male and female tissues examined, with the highest expression level observed in brain. [score:3]
Our qRT-PCR analysis further confirmed that the identified O. melastigma miRNAs (let-7a, miR-9-3p, miR-122, miR-27a and miR-2184) are authentic miRNAs, which are differentially expressed by specific tissue and sex. [score:3]
qRT-PCR analysis demonstrated the suppression of let-7a in female liver, male liver and female brain under hypoxia, respectively (A–C). [score:3]
In gonads, the most highly expressed miRNA was let-7a, with RPM values of 3,563 in ovary and 2,351 in testis (Tables S6– S7). [score:3]
Other highly expressed miRNAs in brain included let-7a, miR-29b and miR-153, each having RPM values greater than 6,000. [score:3]
For example, the first known miRNAs, lin-4 and let-7, were found to play a major role in developmental timing [30], [31]. [score:2]
Let-7 is one of the most highly expressed across all tissues in our data. [score:2]
For example, mature let-7 regulates cell proliferation and differentiation and is also highly conserved across animal species [32]. [score:2]
Furthermore, let-7 regulated ageing of testis stem cell niche in drosophila [35]. [score:2]
Our results demonstrated a significant suppression of let-7a in hypoxic liver and brain tissues of female marine medaka as compared to normoxic tissues, and a similar reduction of let-7a was also found in male liver (Figure 5A–C). [score:2]
However, the expression levels of let-7a in gonads were fairly low compared to those in brain tissue: 13,722 RPM in females and 10,299 RPM in males. [score:2]
There is ample reports that demonstrated the importance of let-7 in different biological functions of brain, liver and gonads [33]– [35]. [score:1]
Sabrina et al reported that the introduction of let-7 caused the activation of Toll-like receptor 7, resulting in neurodegeneration in mouse’s brain [33]. [score:1]
Our qRT-PCR analysis further identified the hypoxia-responsive miRNAs: let-7a & miR-122 (female liver); let-7a & miR-9-3p (female brain) and miR2184 (testis) in hypoxia-exposed marine medaka. [score:1]
In the brain tissue, 89 miRNAs were identified, 9 of which (miR-20b, miR-83, miR-124-5p, miR-133, miR-184, miR-203a, miR-205a, miR-725, miR-962-3p) are only found in female brain and 3 (let-7a-2-3p, miR-17-3p, miR-124-3p) are enriched in male brain (Figure 4a). [score:1]
But, it has been reported that let-7 can be induced by HIF-1α in response to hypoxia in vascular endothelial cell (HUVECs) [18]. [score:1]
This response to oxygen depletion is concordant to the previous finding that hypoxia caused reduction of let-7a in nasopharyngeal carcinoma [46]. [score:1]
Experiments were therefore further carried out to test the response of five selected miRNA (let-7a, miR-9-3p, miR-27a, miR-122 and miR-2184) to hypoxic stress. [score:1]
However, there is no significant change of let-7a in gonadal tissues and male brain of marine medaka (data not shown). [score:1]
Also, let-7 was found to control glucose homeostasis and insulin sensitivity in liver [34]. [score:1]
Only 3 O. melastigma miRNAs (Let-7, miR-1 and miR-124) were highly conserved across all mo del animals, 52 were conserved in all of the vertebrate mo dels, and 31 were found to be teleost-specific (Table 4). [score:1]
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2
[+] score: 2
Other miRNAs from this paper: dre-mir-10a, dre-mir-10b-1, dre-mir-183, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-mir-1-2, dre-mir-9-1, dre-mir-9-2, dre-mir-9-4, dre-mir-9-3, dre-mir-9-5, dre-mir-9-6, dre-mir-9-7, dre-mir-10b-2, dre-mir-10c, dre-mir-10d, dre-mir-15a-1, dre-mir-15a-2, dre-mir-17a-1, dre-mir-17a-2, dre-mir-20a, dre-mir-29b-1, dre-mir-29b-2, dre-mir-29a, dre-mir-92a-1, dre-mir-92a-2, dre-mir-92b, dre-mir-101a, dre-mir-101b, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-145, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-2, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-mir-499, ola-mir-430a-1, ola-mir-430c-1, ola-mir-430b-1, ola-mir-430c-2, ola-mir-430c-3, ola-mir-430d-1, ola-mir-430a-2, ola-mir-430c-4, ola-mir-430d-2, ola-mir-430a-3, ola-mir-430a-4, ola-mir-430c-5, ola-mir-430d-3, ola-mir-430b-2, ola-mir-430c-6, ola-mir-430c-7, ola-mir-20a-1, ola-mir-92a-2, ola-mir-9a-2, ola-mir-101a, ola-mir-9b-1, ola-mir-499, ola-let-7a-1, ola-mir-9a-3, ola-mir-183-1, ola-mir-29b-1, ola-mir-29a, ola-mir-124-1, ola-mir-124-2, ola-mir-9a-4, ola-mir-101b, ola-let-7a-4, ola-mir-10d, ola-mir-9a-1, ola-mir-92b, ola-mir-9b-2, ola-mir-1-2, ola-mir-124-3, ola-mir-15a, ola-mir-10b, ola-mir-92a-1, ola-mir-20a-2, ola-mir-17, ola-mir-29b-2, ola-mir-29c, ola-mir-183-2, ola-let-7a-3, ola-mir-9a-5, ola-mir-145, dre-mir-29b3
For detailed lists of miRNA family assignments, see Supplement Table 4 The age -dependent expression of the following miRNAs was previously demonstrated by qPCR: tni-miR-15a, tni-miR-101a, tni-miR-101b, dre-miR-145, hsa-miR 29c-1 (100% identical to dre-miR-29a), hsa-let-7a-5p, hsa-miR-124a-1, hsa-miR-1-2, olamiR-21, ola-miR-183-5p and, from cluster dre-miR-17a/18a/19a, and dre-miR-20a (the used primers were Qiagen miScript primer). [score:1]
For detailed lists of miRNA family assignments, see Supplement Table 4 The age -dependent expression of the following miRNAs was previously demonstrated by qPCR: tni-miR-15a, tni-miR-101a, tni-miR-101b, dre-miR-145, hsa-miR 29c-1 (100% identical to dre-miR-29a), hsa-let-7a-5p, hsa-miR-124a-1, hsa-miR-1-2, olamiR-21, ola-miR-183-5p and, from cluster dre-miR-17a/18a/19a, and dre-miR-20a (the used primers were Qiagen miScript primer). [score:1]
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