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6 publications mentioning ssc-mir-218-2

Open access articles that are associated with the species Sus scrofa and mention the gene name mir-218-2. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 142
Based on our results, we speculate that the α-(1, 2) fucosyltransferase 2 gene (FUT2) and Discs, large homolog 5 (DLG5) genes were the targets of down-regulated ssc-miR-218-3p, the MUC4 gene was the target of down-regulated ssc-miR-136, MyD88 was the target of up-regulated ssc-miR-499-5p, LBP and Toll-like receptor (TLR4) genes were the target of up-regulated ssc-miR-196b. [score:21]
Based on our results, we speculate that the α-(1, 2) fucosyltransferase 2 gene (FUT2) and Discs, large homolog 5 (DLG5) genes were the targets of down-regulated ssc-miR-218-3p, the MUC4 gene was the target of down-regulated ssc-miR-136, MyD88 was the target of up-regulated ssc-miR-499-5p, LBP and Toll-like receptor (TLR4) genes were the target of up-regulated ssc-miR-196b. [score:21]
Interestingly, RT-qPCR showed that ssc-miR-136 and ssc-miR-218-3p were significantly up-regulated in F18-sensitive piglets (P < 0.05), and ssc-miR-196b and ssc-miR-499-5p were significantly up-regulated in F18-resistant piglets (P < 0.05). [score:7]
Based on the expression correlation between miRNA and target genes analysis, we speculate that miR-218-3p targeting to DLG5, appears to be very promising candidate for miRNAs involved in response to E. coli F18 infection. [score:7]
They show that mir-218-3p is down-regulated in sensitive tissues by RT-PCR (Table 1), and DLG5 is also down-regulated in the same tissue (fig. 8), yet they assert that the two are strongly NEGATIVELY correlated (fig. 9). [score:7]
It seems that there is a fundamental discrepancy between sequencing data (fig. 5) which shows that mir-218 is UP-regulated in sensitive tissues and the RT-PCR data (Table 1) which shows a significant DOWN-regulation in the same tissue, thus not a validation but instead in opposition. [score:7]
Based on the expression correlation between miRNA and potential target mRNAs, we speculate that DLG5, potential target gene of miR-218-3p, probably acts as a novel marker of E. coli F18 resistance. [score:7]
In the present study, RT-qPCR showed that the expression of miR-136, miR-218-3p, miR-196b and miR-499-5p were significantly up- or down-regulated between F18-sensitive and -resistant piglets. [score:6]
*means the difference was significant in the test level of P < 0.05 and **means extremely significant in 0.01 level In this study, we analysed the correlation between expression of miR-218-3p and six target genes (Fig.   9). [score:5]
However, the expression level of the miR-218-3p had significant positive correlations with LBP and MyD88 expression (P < 0.05). [score:5]
Previous studies have shown that above miRNAs (miR-136, miR-218-3p, miR-196b and miR-499-5p) indeed play roles in the development and regulation of human disease [32– 35]. [score:5]
Fig. 9Correlation analysis of gene expression of miR-218-3p with six potential target mRNAs in intestinal tissue. [score:5]
The expression level of miR-218-3p in intestinal tissue had a very significant negative correlation with DLG5 (P < 0.01), but there were no significant correlations with FUT2, MUC4 and TLR4 expression. [score:5]
Interestingly, the expression of miR-218-3p in intestinal tissue had a very significant negative correlation with target DLG5 (P < 0.01). [score:5]
These studies will further our understanding of the mechanisms of miR-218-3p as well as miRNA -mediated genes in the regulation of Mershan weaning piglets resistance to E. coli F18 and lay a solid foundation for the breeding of disease resistance to E. coli in Chinese domestic pig breeds. [score:4]
Therefore, we speculated that miR-218-3p targeting DLG5 was likely to regulate the formation of the E. coli F18 receptor and maintain the intestinal mucosa integrity of epithelial cells. [score:4]
Stem-loop RT-qPCR showed that miR-136, miR-196b, miR-499-5p and miR-218-3p significantly expressed in intestinal tissue (p < 0.05). [score:3]
This paper suggests that several miRNAs, notably mir-218-3p, are involved in E. Coli resistance in Meishan piglets, via targeting DLG5 among others. [score:3]
These results suggested that DLG5 was probably an important target gene of miR-218-3p. [score:3]
Author’s response: In our manuscript, we performed a comparative miRNA sequencing of duodenal tissues between E. coli F18-resistant group and E. coli F18-sensitive group, and then we screened out some differential expression miRNAs including mir-218-3p. [score:3]
In our study, the expression level of miR-218-3p in intestinal tissue had a very significant negative correlation with DLG5 (P < 0.01). [score:3]
Meanwhile, we could further analyze the correlation of mir-218-3p and DLG5 expression. [score:3]
In conclusion, we initially identified miR-196b, miR-499-5p and miR-218-3p as candidate miRNAs involved in E. coli F18 infection by miRNAs sequencing and qRT-PCR validation. [score:1]
However, the comparison of miR-SEQ and qRT-PCR has been shown in Fig. 5. 7/The authors should ideally demonstrate that mir-218-3p and DLG5 are both expressed in gut epithelial cells, which is not a given since they carried out measurements on entire duodenal tissue. [score:1]
However, the comparison of miR-SEQ and qRT-PCR has been shown in Fig. 5. 7/The authors should ideally demonstrate that mir-218-3p and DLG5 are both expressed in gut epithelial cells, which is not a given since they carried out measurements on entire duodenal tissue. [score:1]
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2
[+] score: 17
miR-218 was considered as one of tumor suppressive miRNAs, and involved in modulation of the nuclear factor-kappa B (NF-κB) signaling pathway by directly targeting the IKK-β (IκBs kinase) gene and in activation of the mTOR (mammalian target of rapamycin)-Akt signaling pathway targeting the mTOR component Rictor [57], [58]. [score:9]
Only 3 miRNAs (miR-155, miR-150-1 and miR-204) at W1 and W4, and 4 miRNAs (miR-132, miR-146b, miR-212 and miR-218-2) at W4 and W7 were consistently upregulated across two of three time points. [score:4]
In our study, other 6 miRNAs (miR-155, miR-150-1, miR-204, miR-132, miR-212 and miR-218-2) were consistently upregulated across two of three weaning times. [score:4]
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3
[+] score: 8
For example, hsa-miR-133a, hsa-miR-200b, hsa-miR-206, and hsa-miR-218 were considered as tooth tissue-specific miRNAs [4]; eight differentially expressed miRNAs were expressed during morphogenesis and seven were expressed in the incisor cervical loop containing the stem cell niche [1]; the three most highly expressed microRNAs in dental epithelium were identified as mmu-miR-24, mmu-miR-200c, and mmu-miR-205, while mmu-miR-199a-3p and mmu-miR-705 were found in dental mesenchyme [2]; and miR-200 was suggested to play an important role in the formation of incisor cervical loop during stem cell–fueled incisor growth [5]. [score:8]
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4
[+] score: 4
Among these DE miRNAs, 14 were co-expressed, including miR-218-5p and miR-34c. [score:3]
Five central node genes (CTNNA2, ITGB1, ITGA4, LIMS1 and COL6A2) and two miRNAs (miR218-5p and miR-221-5p) were detected in the integrated network. [score:1]
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5
[+] score: 2
Using miRNA microarray analysis and RT-PCR, some researchers have found that miR-24, miR-31, miR-140, miR-141, miR-205, miR-200c, miR-875-5p, miR-455, miR-689, miR-711, and miR-720 may regulate tooth epithelial stem cell differentiation [6], [7]; others identified miR-133a, miR-200b, miR-206, and miR-218 as tooth-specific miRNAs, and that miR-141, miR-199b*, miR-200a, miR-200b, miR-200c, and miR-429 likely play a role in the renewal and differentiation of adult stem cells during stem cell-fueled incisor growth [8], [9]. [score:2]
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6
[+] score: 1
Among them, six microRNAs (ssc-miR-128, ssc-miR-186, ssc-miR-4332, ssc-miR-218-3p, ssc-miR-2320-5p and ssc-miR-150) were predicted to bind to the 3′UTR of WUH3 PRRSV genome. [score:1]
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