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1 publications mentioning chi-mir-363

Open access articles that are associated with the species Capra hircus and mention the gene name mir-363. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 128
miR-328 was found downregulated in goats but upregulated in sheep; two miRNAs—miR-2285f-2 and miR-27a-5p were found upregulated in goats but downregulated in sheep; six miRNAs—miR-320a-1, miR-320a-2, miR-1246, miR-363, miR-760-3p, and miR-21-3p were upregulated and two miRNAs—miR-34b and miR-150 were downregulated in both species (Figure 1B and Table 3). [score:19]
miRNAs Role Tissue Reference miR-27a-5p Repression of viral replication Lung Roberts et al., 2011b miR-21-3p Induce apoptosis Lung Lo et al., 2013 miR-320a Inhibit virus infection Lung Sun et al., 2014 miR-1246 Promotes virus cytotoxicity Lung Sheng et al., 2014 miR-363 Induced apoptosis Lung and spleen Zhang et al., 2014 miR-760-3p Very highly upregulated Lung and spleen – FIGURE 2 Overview of the miRNA prediction and identification of miRNAs that regulated dysregulated proteins. [score:8]
miRNAs Role Tissue Reference miR-27a-5p Repression of viral replication Lung Roberts et al., 2011b miR-21-3p Induce apoptosis Lung Lo et al., 2013 miR-320a Inhibit virus infection Lung Sun et al., 2014 miR-1246 Promotes virus cytotoxicity Lung Sheng et al., 2014 miR-363 Induced apoptosis Lung and spleen Zhang et al., 2014 miR-760-3p Very highly upregulated Lung and spleen – FIGURE 2 Overview of the miRNA prediction and identification of miRNAs that regulated dysregulated proteins. [score:8]
The upregulated miR-27a-5p, miR-363, miR-320a, and miR-760-3p were observed to bring about the downregulation of IGF1R in the PPRV infected lung of goats. [score:7]
miR-363 downregulates Mcl-1 expression and induces apoptosis in HepG2 cells. [score:6]
microRNA-363 plays a tumor suppressive role in osteosarcoma by directly targeting MAP2K4. [score:6]
Similarly, the expression of IFIT5, which is involved in stimulating anti-viral response (Zhang et al., 2013) was suppressed by miR-363 in the spleen of sheep. [score:5]
In spleen, the expression profile of miR-363 was higher in goats (log [2]FC = 1.55) than in sheep (log [2]FC = 1.28), however, no difference in expression of miR-760-3p was observed in goats (log [2]FC = 1.63) and sheep (log [2]FC = 1.67) infected with PPRV. [score:5]
These miRNAs include miR-21-3p, miR-320a, miR-27a-5p, and miR-1246—expressed in lung of both species; miR-760-3p and miR-363—expressed in lung and spleen of both species (Figure 2). [score:5]
Among these 31 DEmiRNAs, six DEmiRNAs—miR-21-3p, miR-320a, miR-27a-5p, miR-1246 (expressed in lung of both species), miR-760-3p and miR-363 (expressed in lung and spleen of both species) were selected based on their role in viral infection, apoptosis and fold change. [score:5]
MicroRNA-363 -mediated downregulation of S1PR1 suppresses the proliferation of hepatocellular carcinoma cells. [score:5]
The upregulation of miR-363 has also been reported in Human papillomavirus (HPV)+ and HPV- pharyngeal squamous cell carcinoma and in HPV16+ HNSCC cell lines (Lajer et al., 2011; Wald et al., 2011). [score:4]
In PPRV infected spleen of goats, miR-363 and miR-760-3p regulate apoptotic molecules (NFATC2, NOX5, and MYO6) and target genes involved in immunological processes (NFATC2, IGF1R, KLHL21, and NOX5) (Figure 3C). [score:4]
This suggests that PPRV -induced miR-21-3p, miR-320a, and miR-363 might act cooperatively to enhance viral pathogenesis in the lung and spleen of sheep by downregulating several immune response genes. [score:4]
The upregulation of miR-21-3p and miR-363 in PPRV infections suggests synergistic effect of these miRs along with the virus in inducing apoptosis. [score:4]
The miRNAs—miR-363 and miR-760-3p commonly present in PPRV infected lung and spleen of both species were identified to be upregulated. [score:4]
Similarly, IGF1R (insulin like growth factor 1) protein, involved in regulation of immune response was the target of miR-27a-5p, miR-363, miR-320a, and miR-760-3p. [score:4]
The upregulated miR-363 is also known to induce apoptosis (Zhang et al., 2014; Zhou et al., 2014; Hu et al., 2015; Li et al., 2015). [score:4]
The immune effector molecules IFIT5 and TRIM33 were targeted by miR-363, and CD244 and NFKBIE were regulated by miR-760-3p in infected spleen of sheep (Figure 3D). [score:4]
To further validate the expression of DEmiRNAs from high-throughput sequencing, qPCR was performed on three DEmiRNAs—miR-21-3p, miR-363, and miR-760-3p. [score:3]
In lung, the expression of miR-363 and miR-760-3p was higher in goats with log [2] fold change of 2.42 and 3.79, respectively, than in sheep (miR-363, log [2]FC = 1.04 and miR-760-3p, log [2]FC = 1.24) after PPRV infection. [score:3]
The expression of miR-363 and miR-760-3p in sheep and goats in both the tissues was in concordance with small RNA sequencing results. [score:3]
Lung (goats)Lung (sheep)Spleen (goats)Spleen (sheep) miRNAsqPCR (log [2] fold changemiRNA-seq (log [2] fold change)qPCR (log [2] fold change)miRNA-seq (log [2] fold change)qPCR (log [2] fold change)miRNA-seq (log [2] fold change)qPCR (log [2] fold change)miRNA-seq (log [2] fold change) miR-21-3p +6.619 +5.826 +4.344 +1.753 +0.189 – +0.097 – miR-363 +5.823 +2.425 +0.345 +1.049 +0.618 +1.555 +0.474 +1.286 miR-760-3p +5.433 +3.798 +0.629 +1.242 +2.199 +1.637 +1.784 +1.679 PPR is a major threat to livestock keepers in developing countries, causing a severe disease in goats and sheep. [score:3]
In the miRNA–protein network of lung tissue of goats, three miRNAs—miR-21-3p, miR-363, and miR-320a mutually regulate EGFR (epidermal growth factor receptor), which is involved in immune response. [score:2]
The change in the miRNA expression of miR-21-3p, miR-363, and miR-760-3p was calculated with U6snRNA as reference gene for normalization. [score:1]
Lung (goats)Lung (sheep)Spleen (goats)Spleen (sheep) miRNAsqPCR (log [2] fold changemiRNA-seq (log [2] fold change)qPCR (log [2] fold change)miRNA-seq (log [2] fold change)qPCR (log [2] fold change)miRNA-seq (log [2] fold change)qPCR (log [2] fold change)miRNA-seq (log [2] fold change) miR-21-3p +6.619 +5.826 +4.344 +1.753 +0.189 – +0.097 – miR-363 +5.823 +2.425 +0.345 +1.049 +0.618 +1.555 +0.474 +1.286 miR-760-3p +5.433 +3.798 +0.629 +1.242 +2.199 +1.637 +1.784 +1.679 Viral infection in the lung and spleen of sheep and goats infected with PPRV was confirmed by RT-PCR of 351 bp N gene amplicon in lung and spleen (Supplementary Figure S1). [score:1]
This study revealed that PPRV -induced miR-21-3p, miR-320a, and miR-363 might act cooperatively to enhance viral pathogenesis, which warrants further research. [score:1]
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