Accession
MI0014161
Symbol
HGNC:
MIR3138
Description
Homo sapiens
hsa-mir-3138 precursor miRNA mir-3138
Gene
family?
family?
RF03837;
mir-3138
Summary
Caution, this is an AI generated summary based on literature. This may have errors. ?
MIR3138 is a microRNA that has been implicated in nerve degeneration and regeneration in diabetic peripheral neuropathy (DPN) [PMC6615525]. The study used pyrosequencing and RT-PCR to validate changes in methylation and gene expression of DPYSL2 and MIR3138 [PMC6615525]. The researchers cross-referenced predicted and known targets of MIR3138 from miRDB and DIANA-TarBase, identifying ERBB4 as a target of MIR3138 [PMC6615525]. ERBB4 has been associated with sensory nerve loss and regeneration, suggesting a potential regulatory role for MIR3138 in DPN [PMC6615525'>PMC6615525'>PMC6615525'>PMC6615525'>PMC6615525]. The study confirmed that differential methylation seen by RRBS (reduced representation bisulfite sequencing) and pyrosequencing was associated with a decrease in MIR3138 gene expression in degenerating sural nerves [PMC6615525]. DPYSL2, NFATC1, and MIR3138 were validated using pyrosequencing and RT-PCR, suggesting that epigenetic downregulation of MIR3138 may play a role in DPN progression [PMC6615525]. The primers for DPYSL2, NFATC1, and MIR3138 were designed at the University of Michigan Epigenomics Core using a published protocol [PMC6615525]. Furthermore, the study found that MIR3138 was the most differentially methylated miRNA between degenerating nerves and regenerating nerves [PMC6615525]. DPYSL2 showed significantly higher methylation levels in the degenerating group compared to the regenerating group by pyrosequencing (p = 0.0005), similar to the results observed for MIR3138 (p = 0.0130) [PMC6615525].
Literature search
4 open access papers mention hsa-mir-3138
(16 sentences)
(16 sentences)
Sequence
557
reads,
31
reads per million, 53 experiments
cccuccucggcacuucccccaccucacugcccgggugcccacaagacUGUGGACAGUGAGGUAGAGGGAGUgccgaggaggg
(((((((((((((((((.(.((((((((((((((............))).)).))))))))).).)))))))))))))))))
(((((((((((((((((.(.((((((((((((((............))).)).))))))))).).)))))))))))))))))
Structure
c c - - gugcc
cccuccucggcacuucc c accucacug cc cgg c
||||||||||||||||| | ||||||||| || |||
gggaggagccgUGAGGG G UGGAGUGAC GG GUc a
A A A U agaac
Annotation confidence
Not enough data
Do you think this miRNA is real?
Genome context
chr4: 10078611-10078692 [-]
Database links
Mature hsa-miR-3138
| Accession | MIMAT0015006 |
| Description | Homo sapiens hsa-miR-3138 mature miRNA |
| Sequence | 48 - UGUGGACAGUGAGGUAGAGGGAGU - 71 |
| Evidence |
experimental
Illumina [1-2] |
| Database links |
|
| Predicted targets |
|
References
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