Stem-loop cel-mir-267

Accession

MI0000347

Description

Caenorhabditis elegans cel-mir-267 precursor miRNA

Sequence

uaaaaguuucaaaacCCCGUGAAGUGUCUGCUGCAaucuaaauuuuuagauauagaugcagucgacucuucauuggguuuucaggaacccgua
.....((((((((((((.((((((.(((.((((((.((((.(((....))).)))))))))).))).)))))).)))))))..))))).....

Structure

uaaaa     --       C      U   U      a    a   u 
     guuuc  aaaacCC GUGAAG GUC GCUGCA ucua auu u
     |||||  ||||||| |||||| ||| |||||| |||| |||  
     caagg  uuuuggg uacuuc cag ugacgu agau uag u
augcc     ac       u      u   c      -    a   a 

Annotation confidence Not enough data

Comments

This precursor sequence was predicted by comparative computational approaches. The excised miRNA sequence is predicted and the precise 5' or 3' end unknown. A PCR amplification protocol confirmed that the strand containing the predicted miR is predominantly expressed.

Genome context

chrII: 12638134-12638226 [-]

Mature cel-miR-267

• Accession: MIMAT0000323
• Description: Caenorhabditis elegans cel-miR-267 mature miRNA
• Sequence: 16 - CCCGUGAAGUGUCUGCUGCA - 35
• Evidence: experimental ; PCR [1]

References

1. PubMed ID: 12769849
   Computational and experimental identification of C. elegans microRNAs
   "Grad Y, Aach J, Hayes GD, Reinhart BJ, Church GM, Ruvkun G, Kim J"
   "Mol Cell (2003) 11:1253-1263