miRBase entry: dme-mir-278

Stem-loop dme-mir-278


Accession
MI0000361
Description
Drosophila melanogaster dme-mir-278 precursor miRNA
Gene family
MIPF0000155; mir-278

Literature search
6 open access papers mention dme-mir-278
(13 sentences)

Sequence

121023 reads, 4144 reads per million, 49 experiments
guaaugguacggugcgaCCGGAUGAUGGUUCACAACGACCgugucauuuaaacgggUCGGUGGGACUUUCGUCCGUUUguaacgccauuugucaacga
..((((((....(((((.(((((((.(((((.((.(((((.(((.......)))))))).))))))).))))))).)))))..)))))).........

Structure
-------gu      acgg     C       U     A  A     g   ca 
         aauggu    ugcga CGGAUGA GGUUC CA CGACC ugu  u
         ||||||    ||||| ||||||| ||||| || ||||| |||  u
         uuaccg    augUU GCCUGCU UCAGG GU GCUgg gca  u
agcaacugu      --ca     U       U     -  G     -   aa 


Annotation confidence High
Do you think this miRNA is real?
Comments
miR-278 was reported independently in references [1] and [2]. Computational prediction followed by northern blotting confirmed that the strand containing the predicted miR is predominantly expressed [1]. Reference [2] confirmed the 5' end of the excised miRNA by cloning and reported a length distribution of 20-22 nt with 22 nt the most commonly expressed.

Genome context
chr2R: 15657066-15657163 [+]

Database links

Mature dme-miR-278-3p

Accession MIMAT0000339
Description Drosophila melanogaster dme-miR-278-3p mature miRNA
Sequence 57 - UCGGUGGGACUUUCGUCCGUUU - 78
Evidence experimental
Northern [1-2], cloned [2], 454 [3-4], Illumina [4]
Database links
Predicted targets

Mature dme-miR-278-5p

Accession MIMAT0020805
Description Drosophila melanogaster dme-miR-278-5p mature miRNA
Sequence 18 - CCGGAUGAUGGUUCACAACGACC - 40
Evidence not_experimental
Database links

References

  1. PubMed ID: 12919683
    The small RNA profile during Drosophila melanogaster development
    "Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T"
    "Dev Cell (2003) 5:337-350

  2. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  3. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  4. PubMed ID: 12844358
    Computational identification of Drosophila microRNA genes
    "Lai EC, Tomancak P, Williams RW, Rubin GM"
    "Genome Biol (2003) 4:R42