miRBase entry: hsa-mir-195

Stem-loop hsa-mir-195


Accession
MI0000489
Symbol
HGNC: MIR195
Description
Homo sapiens hsa-mir-195 precursor miRNA
Gene family
MIPF0000006; mir-15

Summary
Caution, this is an AI generated summary based on literature. This may have errors. ?

MIR195 is a subset of miRNAs that has been studied in relation to its expression in MIN6B1 cells and rat pancreatic islets. Agarose gel electrophoresis was conducted at different N/P ratios to confirm the condensation of MIR195 with FasudilSHPMIR195 [PMC4990390]. The expression of MIR195, along with other miRNAs such as miR34a, miR96, miR145, miR146, and miR210, was further analyzed using quantitative RT-PCR in a large series of samples obtained from MIN6B1 cells and freshly isolated rat pancreatic islets [PMC2551683]. 

References:
- [PMC4990390]: The reference provides information on the agarose gel electrophoresis conducted at different N/P ratios to confirm the condensation of MIR195 with FasudilSHPMIR195.
- [PMC2551683]: The reference provides information on the analysis of the expression of MIR195 and other miRNAs using quantitative RT-PCR in samples obtained from MIN6B1 cells and rat pancreatic islets.

Literature search
231 open access papers mention hsa-mir-195
(1860 sentences)

Sequence

191769 reads, 1198 reads per million, 153 experiments
agcuucccuggcucUAGCAGCACAGAAAUAUUGGCacagggaagcgagucugCCAAUAUUGGCUGUGCUGCUCCaggcaggguggug
.(((.(((((.((..((((((((((.((((((((((..((........))))))))))))..))))))))))..)).))))).))).

Structure
a   u     g  cU          -A          ca  gaa 
 gcu cccug cu  AGCAGCACAG  AAUAUUGGCa  gg   g
 ||| ||||| ||  ||||||||||  ||||||||||  ||    
 ugg gggac ga  UCGUCGUGUC  UUAUAACCgu  cu   c
g   u     g  CC          GG          --  gag 


Annotation confidence High
Do you think this miRNA is real?
Comments
This miRNA sequence is predicted based on homology to a verified miRNA from mouse [1], later verified in human [2].

Genome context
chr17: 7017615-7017701 [-]
Clustered miRNAs
1 other miRNA is < 10 kb from hsa-mir-195
Name Accession Chromosome Start End Strand Confidence




Disease association
hsa-mir-195 is associated with one or more human diseases in the Human microRNA Disease Database
Disease Description Category PubMed ID


Database links

Mature hsa-miR-195-5p

Accession MIMAT0000461
Description Homo sapiens hsa-miR-195-5p mature miRNA
Sequence 15 - UAGCAGCACAGAAAUAUUGGC - 35
Evidence experimental
cloned [2]
Database links
Predicted targets

Mature hsa-miR-195-3p

Accession MIMAT0004615
Description Homo sapiens hsa-miR-195-3p mature miRNA
Sequence 53 - CCAAUAUUGGCUGUGCUGCUCC - 74
Evidence experimental
cloned [2]

References

  1. PubMed ID: 17604727
    A mammalian microRNA expression atlas based on small RNA library sequencing
    "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
    "Cell (2007) 129:1401-1414

  2. PubMed ID: 12554859
    New microRNAs from mouse and human
    "Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T"
    "RNA (2003) 9:175-179