miRBase entry: mmu-mir-1a-2

Stem-loop mmu-mir-1a-2


Accession
MI0000652
Symbol
MGI: Mir1a-2
Description
Mus musculus mmu-mir-1a-2 precursor miRNA
Gene family
MIPF0000038; mir-1

Literature search
287 open access papers mention mmu-mir-1a-2
(2998 sentences)

Sequence

20208824 reads, 19830 reads per million, 99 experiments
ucagagcACAUACUUCUUUAUGUACCCAUAugaacauucagugcuaUGGAAUGUAAAGAAGUAUGUAUuuug
.(((((.((((((((((((((((..(((((.(.((.....)).)))))).)))))))))))))))).)))))

Structure
u     c                AC     u a  a 
 cagag ACAUACUUCUUUAUGU  CCAUA g ac u
 ||||| ||||||||||||||||  ||||| | || u
 guuuU UGUAUGAAGAAAUGUA  GGUau c ug c
-     A                -A     - g  a 


Annotation confidence High
Do you think this miRNA is real?
Comments
Lagos-Quintana et al. [1] reported the cloning of miR-1b, miR-1c and miR-1d. The mature processed miR sequences are identical apart from the 3' residues (A in mir-1b, C in mir-1c and UU in mir-1d). The 3' residues of both miR-1b and miR-1c conflict with the predicted stem-loop precursor sequence shown here and these sequences are not found in current assemblies of human and mouse genomes. It is suggested that polyA polymerase may add 1-3 nts to the 3' end of the mature transcript (Tom Tuschl, pers. comm.). The common 21 nts of the 3 reported miR sequences have been rationalised here and named miR-1. There are 2 pairs of orthologous putative hairpin precursor structures named mir-1-1 (human MIR:MI0000651, mouse MIR:MI0000139), and mir-1-2 (human MIR:MI0000437, mouse MIR:MI0000652). The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [2].

Genome context
chr18: 10785481-10785552 [-]
Clustered miRNAs
2 other miRNAs are < 10 kb from mmu-mir-1a-2
Name Accession Chromosome Start End Strand Confidence




Database links

Mature mmu-miR-1a-3p

Accession MIMAT0000123
Description Mus musculus mmu-miR-1a-3p mature miRNA
Sequence 47 - UGGAAUGUAAAGAAGUAUGUAU - 68
Evidence experimental
cloned [1-2], Illumina [3]
Database links
Predicted targets

Mature mmu-miR-1a-2-5p

Accession MIMAT0017047
Description Mus musculus mmu-miR-1a-2-5p mature miRNA
Sequence 8 - ACAUACUUCUUUAUGUACCCAUA - 30
Evidence experimental
Illumina [3]
Database links
Predicted targets

References

  1. PubMed ID: 17604727
    A mammalian microRNA expression atlas based on small RNA library sequencing
    "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
    "Cell (2007) 129:1401-1414

  2. PubMed ID: 12007417
    Identification of tissue-specific microRNAs from mouse
    "Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T"
    "Curr Biol (2002) 12:735-739

  3. PubMed ID: 20413612
    Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
    "Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP"
    "Genes Dev (2010) 24:992-1009