Stem-loop sequence rno-mir-421

Accession

MI0001423 (change log)

Description

Rattus norvegicus miR-421 stem-loop

Gene family

MIPF0000098; mir-95

Literature search

6 open access papers mention rno-mir-421
(17 sentences)

Stem-loop

       cu      -    uua            a   aaa 
5' caca  guaggc cuca   aauguuuguuga uga   a
   ||||  |||||| ||||   |||||||||||| |||   a
3' gugu  cguccg gggu   uuacagacaacu acu   u
       cu      c    uaa            -   aag 

Deep sequencing

125540 reads, 6.88 reads per million, 494 experiments

Confidence

Annotation confidence: high

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Genome context

• Coordinates (Rnor_6.0; GCA_000001895.4): chrX: 74443492-74443570 [-]
• Overlapping transcripts:

  intergenic

Clustered miRNAs

< 10kb from rno-mir-421

• rno-mir-374: chrX: 74443648-74443715 [-]
• rno-mir-421: chrX: 74443492-74443570 [-]

Database links

• EntrezGene:Mir421
• miRBase Tracker:MI0001423

Mature sequence rno-miR-421-5p

• Accession: MIMAT0001320
• Previous IDs: rno-miR-421
• Sequence: 10 - ggccucauuaaauguuuguug - 30
• Deep sequencing: 527 reads, 186 experiments
• Evidence: experimental; cloned [1], SOLiD [2]
• Predicted targets:
  • TargetScanVert:rno-miR-421-5p
  • miRDB:rno-miR-421-5p
  • microrna.org:rno-miR-421-5p

Mature sequence rno-miR-421-3p

• Accession: MIMAT0017175
• Previous IDs: rno-miR-421*
• Sequence: 47 - aucaacagacauuaauuggg - 66
• Deep sequencing: 124995 reads, 494 experiments
• Evidence: experimental; SOLiD [2]
• Predicted targets:
  • TargetScanVert:rno-miR-421-3p
  • miRDB:rno-miR-421-3p

References

1. PMID:15345052 "Microarray analysis of microRNA expression in the developing mammalian brain" Miska EA, Alvarez-Saavedra E, Townsend M, Yoshii A, Sestan N, Rakic P, Constantine-Paton M, Horvitz HR Genome Biol. 5:R68(2004).
2. PMID:20403161 "Small RNA expression and strain specificity in the rat" Linsen SE, de Wit E, de Bruijn E, Cuppen E BMC Genomics. 11:249(2010).