miRBase entry: ebv-mir-BART20

Stem-loop ebv-mir-BART20


Accession
MI0004993
Description
Epstein Barr virus ebv-mir-BART20 precursor miRNA
Gene family
MIPF0000322; mir-BART20


Sequence

uacaggcguagggccuauugUAGCAGGCAUGUCUUCAUUCCugcguaccgaauggCAUGAAGGCACAGCCUGUUACCauuggcaccuuuuuuccaugua
((((((...((((((.((.(((((((((.(((((((((.((............)).)))))))))..))))))))).)).))).))).....)).))))

Structure
    -  --cgu   -   u  u         -A         U  ugcgu 
uaca gg     agg gcc au gUAGCAGGC  UGUCUUCAU CC     a
|||| ||     ||| ||| || |||||||||  ||||||||| ||      
augu cc     ucc cgg ua CAUUGUCCG  ACGGAAGUA gg     c
    a  uuuuu   a   u  C         AC         C  uaagc 


Annotation confidence Not enough data
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Comments
The arm of the precursor miRNA giving rise to the mature sequence was verified using microarray hybridisation and Northern blot, but the extents of the mature miRNA shown here are predicted and not experimentally determined [1]. This sequence was named miR-BART18 by Grundhoff et al [1]. The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [2]. The ends of the miRNA may be offset with respect to previous annotations.

Genome context
HHV507799: 148319-148417 [+]
Clustered miRNAs
21 other miRNAs are < 10 kb from ebv-mir-BART20
Name Accession Chromosome Start End Strand Confidence




Database links

Mature ebv-miR-BART20-5p

Accession MIMAT0003719
Description Epstein Barr virus ebv-miR-BART20-5p mature miRNA
Sequence 21 - UAGCAGGCAUGUCUUCAUUCC - 41
Evidence experimental
array [1], Northern [1], cloned [2]

Mature ebv-miR-BART20-3p

Accession MIMAT0003720
Description Epstein Barr virus ebv-miR-BART20-3p mature miRNA
Sequence 56 - CAUGAAGGCACAGCCUGUUACC - 77
Evidence experimental
array [1], Northern [1], cloned [2]

References

  1. PubMed ID: 17604727
    A mammalian microRNA expression atlas based on small RNA library sequencing
    "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
    "Cell (2007) 129:1401-1414

  2. PubMed ID: 16540699
    A combined computational and microarray-based approach identifies novel microRNAs encoded by human gamma-herpesviruses
    "Grundhoff A, Sullivan CS, Ganem D"
    "RNA (2006) 12:733-750