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17 publications mentioning gga-mir-223

Open access articles that are associated with the species Gallus gallus and mention the gene name mir-223. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 349
Together with the expression data of miR-223, these results suggesting that miR-223 upregulated its expression during the active stage of muscle cell differentiation and fusion, whereas it downregulated its expression during muscle fiber maturation. [score:13]
miR-223 overexpression inhibited the mRNA and protein expression level of ZEB1 (Figure 5d and e), and the inhibition of miR-223 promoted the mRNA and protein expression level of ZEB1 (Figure 5f and g). [score:11]
For primary myoblast, MYOD loss-of-function not only inhibited the expression of miR-223 and muscle differentiation marker genes, but also promoted the expression of miR-223 target genes IGF2 and ZEB1 (Figure 7f). [score:9]
In addition, in situ hybridization results also showed that the miR-223 expression was upregulated from E10 to E13 and downregulated from E14 to E19 (Figure 1b). [score:9]
miR-223 overexpression can also inhibit the expression of cell cycle-promoting genes and enhance cell cycle-inhibiting genes (Figure 2d). [score:9]
In addition, miR-223 significantly inhibited the mRNA and protein expression of IGF2 gene in CPM (Figure 3c), and the inhibition of miR-223 released IGF2 mRNA and protein expression (Figure 3d). [score:9]
Overexpression of miR-223 significantly increased the expression of muscle differentiation marker genes (Figure 4c and d), whereas the inhibition of miR-223 decreased the expression of these marker genes (Figure 4e and f). [score:9]
ZEB1 is another miR-223 target gene, functioning as an inhibitor of myoblast differentiationBy using the TargetScan online software to predict the target genes of miR-223, we found that the 3′-UTR of ZEB1 mRNA has a potential binding site of miR-223 (Figure 5a). [score:9]
In the differentiating avian myoblast, the upregulation of MYOD promotes miR-223 expression, and miR-223 inhibits ZEB1 rather than IGF2 to facilitate myoblast differentiation and fusion. [score:8]
This expression trend is similar to miR-223, which can directly inhibit IGF2 expression. [score:8]
In the proliferating avian myoblast, miR-223, which is upregulated by MYOD, inhibits IGF2 expression to represses myoblast proliferation. [score:8]
Furthermore, the rescue assay shown that miR-223 overexpression can inhibit the myoblast proliferation, and the transfection of IGF2 overexpression vector could counteract the inhibitory effect of miR-223 on myoblast proliferation (Figure 3q). [score:8]
miR-223 inhibition also enhanced the expression of cell cycle-promoting genes and repressed cell cycle-inhibiting genes (Figure 2f). [score:7]
For ZEB1, miR-223 inhibits its expression in both proliferation and differentiation stages of myoblast (Figure 6g), and the inhibitory effect of miR-223 on ZEB1 has no significant difference between these two stages. [score:7]
In addition, the expression of miR-223 and MYOD are upregulated during chicken myoblast differentiation, [29] demonstrating a positive association between these two regulators. [score:7]
For IGF2, miR-223 inhibits its expression only in the proliferation stage of myoblast (Figure 6g), and the miR-223 expression shown no significant difference between the proliferation and differentiation stages (Figure 6h). [score:7]
[39] Therefore, our results suggesting that MYOD inhibits ZEB1 expression by promoting miR-223 expression during myoblast differentiation. [score:7]
On the other hand, ZEB1, another miR-223 target gene that can inhibit myoblast differentiation, is unable to regulate cell cycle in proliferating myoblast (Figure 6e and f). [score:6]
miR-223 was upregulated its expression during the active stage of chicken skeletal muscle differentiation and fusion. [score:6]
miR-223 expression was significantly upregulated from the proliferation to the differentiation of both CPM and qm-7 cells (Figure 4a and b), suggesting its involvement in these processes. [score:6]
34, 35, 36 Considering that MYOD, MYOG and miR-223 are all upregulated their expression during myoblast differentiation, we next examined whether MYOD and MYOG can bind to these two E-box regions and promote gga-miR-223 transcription. [score:6]
The inhibitory effect of miR-223 on myoblast proliferation was achieved by its target gene IGF2IGF2 is an important growth factor that can functioning as growth promoting hormone during cell development. [score:6]
miR-223 regulates myoblast proliferation and differentiation by balancing the expression of its target genes IGF2 and ZEB1. [score:6]
miR-223 was gradually upregulated its expression from embryo day 10 (E10) to E13. [score:6]
In this study, we have shown that ZEB1 protein was gradually downregulated during chicken myoblast differentiation, and the decrease of ZEB1 is, at least in part, owing to the inhibition of miR-223. [score:6]
The inhibition of miR-223 on IGF2 and ZEB1 is different between myoblast proliferation and differentiationThe above results shown that both IGF2 and ZEB1 are target genes of miR-223. [score:5]
ZEB1 is another miR-223 target gene, functioning as an inhibitor of myoblast differentiation. [score:5]
Therefore, ZEB1 is another miR-223 target gene that can function as an inhibitor of myoblast differentiation. [score:5]
By using the TargetScan online software to predict the target genes of miR-223, we found that the 3′-UTR of ZEB1 mRNA has a potential binding site of miR-223 (Figure 5a). [score:5]
Notably, miR-223 inhibits IGF2 expression only in the proliferating myoblast. [score:5]
Here, we reported that IGF2 can both promote avian myoblast proliferation and differentiation, and we also found that miR-223 has the ability to inhibit IGF2 expression. [score:5]
The inhibitory effect of miR-223 on myoblast proliferation was achieved by its target gene IGF2. [score:5]
In addition, MYOD overexpression promoted miR-223 expression (Figure 7e). [score:5]
Together, these results argue that the inhibitory effect of miR-223 on myoblast proliferation was achieved by its target gene IGF2. [score:5]
indicated that miR-223 overexpression inhibited myoblast proliferation (Figure 2a), whereas miR-223 loss-of-function promoted myoblast proliferation (Figure 2b). [score:5]
The miR-223 mimics, negative control (NC), miR-223 inhibitors, miRNA inhibitor NC, siRNA against chicken IGF2, ZEB1 and MYOD were all purchased from GenePharma (GenePharma, Shanghai, China). [score:5]
[8] To further understand the relationship between miR-223 and skeletal muscle development, we detected its expression in breast muscle during chicken embryonic development. [score:5]
miR-223 expression is related to skeletal muscle development. [score:4]
The above results indicated that IGF2 is a direct target gene of miR-223. [score:4]
Therefore, we were interest in how the miR-223 regulates the ZEB1 and IGF2 expression in the proliferating and differentiating myoblast, respectively. [score:4]
To further determine the roles of miR-223 in chicken skeletal muscle development, we transfected miR-223 mimics and inhibitors into chicken primary myoblast (CPM), respectively. [score:4]
These results suggested that miR-223 cannot restrict IGF2 expression and function during myoblast differentiation. [score:3]
The above results shown that both IGF2 and ZEB1 are target genes of miR-223. [score:3]
The dual-luciferase report assay suggested that miR-223 directly binds to the predicted target site of ZEB1-3′-UTR (Figure 5c). [score:3]
Together, these results suggested that the inhibition of miR-223 on IGF2 and ZEB1 is different between myoblast proliferation and differentiation. [score:3]
28, 34 In this study, we found that MYOD can bind to the E-box, which is located in the promoter region of miR-223 gene, and promote miR-223 expression in chicken myoblast. [score:3]
The inhibition of miR-223 on IGF2 and ZEB1 is different between myoblast proliferation and differentiation. [score:3]
In our previous miRNA sequencing data, we found that miR-223 exhibited differentially expression between the skeletal muscles of chickens with different growth rate. [score:3]
In addition, the inhibitory effect of miR-223 on ZEB1 in the proliferating myoblast was more significant than that in the differentiating myoblast. [score:3]
Therefore, the above results indicated that ZEB1 is another target gene of miR-223. [score:3]
In addition, by analyzing cell cycle of the transfected myoblasts, we found that miR-223 overexpression can decrease cell population in the S phase and increase cell population in the G1/0 phase (Figure 2c). [score:3]
Next, we examined the expression and function of miR-223 during myoblast differentiation and fusion. [score:3]
Therefore, we transfected miR-223 mimic and inhibitor into the myoblasts. [score:3]
To validate whether IGF2 is a target gene of miR-223, we constructed two dual-luciferase reporters with the wide-type and mutant 3′-UTR of IGF2, respectively. [score:3]
Furthermore, these roles of miR-223 were also existed in the qm-7 cells (Figure 2i and p), indicating that miR-223 can inhibit avian myoblast proliferation. [score:3]
miR-223 inhibits myoblast proliferation. [score:3]
Here, we found that the 3′-untranslated regions (3′-UTR) of IGF2 mRNA has a potential binding site of miR-223 (Figure 3a). [score:3]
On the contrary, inhibition of miR-223 significantly increased cell population in the S phase and decreased cell population in the G1/0 phase (Figure 2e). [score:3]
To avoid the effect of endogenous MYOD, we used DF-1 cell to test the regulation of MYOD on the gga-miR-223 gene promoter and miR-223 transcription. [score:2]
Rescue assay shown that ZEB1 overexpression is able to counteract the promotion effect of miR-223 on myoblast differentiation (Figure 5m). [score:2]
Together, these results demonstrated that the MYOD regulates gga-miR-223 transcription by binding to the E-box 1 region. [score:2]
MYOD regulates miR-223 transcription by binding to the E-box 1 region. [score:2]
MYOD regulates miR-223 transcription by binding to the E-box 1 regionTo further understand the structure of the gga-miR-223 gene, we isolated its full-length pri-miR-223 by using 5′ and 3′ rapid amplification of cDNA ends (RACE). [score:2]
[48] In summary, this work has shown that the avian myoblast proliferation and differentiation are regulated by MYOD-miR-223-IGF2/ZEB1 pathway. [score:2]
[49] However, the precise mechanism of how miR-223 dynamic regulates IGF2 and ZEB1 during myoblast proliferation and differentiation still need to be explored. [score:2]
In this study, we identified miR-223 as another miRNA that has a role in avian skeletal muscle development. [score:2]
gga-miR-223 miRCURY LNA probe and a scrambled probe were synthesized (Exiqon, Copenhagen, Denmark). [score:1]
Four fragments, including 668-bp, 1020-bp, 1508-bp and 1932-bp upstream regions of the gga-miR-223 transcription start site (TSS) were amplified and cloned into the pGL3-basic vector. [score:1]
In addition, MYOD promotes miR-223 transcription during myoblast differentiation by binding to the gga-miR-223 gene promoter region (Figure 8). [score:1]
The obtained gga-miR-223 gene was 2228 bp in length (Figure 7a). [score:1]
To further understand the structure of the gga-miR-223 gene, we isolated its full-length pri-miR-223 by using 5′ and 3′ rapid amplification of cDNA ends (RACE). [score:1]
shown that miR-223 significantly repressed the luciferase activity of the wide-type reporter, whereas it has no effect on the luciferase activity of the mutant reporter (Figure 3b). [score:1]
Next, we analyzed the upstream region of the gga-miR-223 gene to find the core promotor region. [score:1]
Therefore, these results demonstrated that miR-223 can promote myoblast differentiation. [score:1]
Wild-type or mutant IGF2-3′ UTR dual-luciferase reporter (200 ng) and miR-223 mimic or NC duplexes (50 nM) were co -transfected into QM-7 cells using the Lipofectamine 3000 reagent (Invitrogen) in 48-well plates. [score:1]
In addition, miR-223 promotes the formation of myotubes (Figure 4g), and the size of the myotube area was significantly increased after transfection of miR-223 (Figure 4h). [score:1]
In addition, we found that there are two E-boxes located in the 1932-bp upstream regions of the gga-miR-223 TSS (Figure 7a). [score:1]
miR-223 promotes myoblast differentiation. [score:1]
miR-223 promoter reporter plasmid. [score:1]
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2
[+] score: 119
Linc-GALMD3 activates its downstream neighboring gene expressionThe expression of up/downstream neighboring genes of linc-GALMD3 were detected by qRT-PCR in MDV-infected and non-infected CD4+ T cells of the two reciprocal cross lines, and the expression of gga-miR-223 and ENSGALG00000004681 were significantly increased after MDV infection in both two lines (p value < 0.01), suggesting that the linc-GALMD3 might activate the expression of its downstream neighboring genes through its cis-regulatory effects; however, the expression of the upstream gene, ENSGALG00000004688, has no obvious change (Fig. 1C; 6 [3] × 7 [2]: p-value = 0.08; and 7 [2] × 6 [3]: p-value = 0.82). [score:12]
means non-infected CD4+ T cells; up/downstream gene expression were both normalized by GAPDH expression, ggg-miR-223 expression was normalized by chicken 5s rRNA expression, all the expression were normalized by the corresponding non-infection group; N = 3; and ** p value < 0.01. [score:11]
The expression of up/downstream neighboring genes of linc-GALMD3 were detected by qRT-PCR in MDV-infected and non-infected CD4+ T cells of the two reciprocal cross lines, and the expression of gga-miR-223 and ENSGALG00000004681 were significantly increased after MDV infection in both two lines (p value < 0.01), suggesting that the linc-GALMD3 might activate the expression of its downstream neighboring genes through its cis-regulatory effects; however, the expression of the upstream gene, ENSGALG00000004688, has no obvious change (Fig. 1C; 6 [3] × 7 [2]: p-value = 0.08; and 7 [2] × 6 [3]: p-value = 0.82). [score:10]
Furthermore, 21 out of these 27 genes were up-regulated after loss function of linc-GALMD3, and previous results showed that the expression of gga-miR-223 was positively regulated by linc-GALMD3 (Figs  1 C and 3F); therefore, these 21 DEGs might be released out when the expression of gga-miR-223 was decreased after loss of function of linc-GALMD3. [score:9]
The expression of downstream gene, ENSGALG00000004681, had an obvious reducing trend as well (Fig.   3F), indicating that the linc-GALMD3 could positively regulate the expression of its two downstream neighboring genes, especially gga-miR-223 expression. [score:8]
In conclusion, we first discovered linc-GALMD3 in chicken MD that could cis-regulate its downstream neighboring gga-miR-223 expression and trans-regulate gene expression on chicken genome, especially regulate the mitochondrial structure related genes. [score:8]
Moreover, Tian et al. utilized microarrays to screen miRNAs that were sensitive to MDV infection, and found that gga-miR-223 was significantly up-regulated in MDV-infected line 7 [2] compared with non-infected line 7 [2] [58], showing that gga-miR-223 was highly expressed in MDV-infected chickens, which was consistent with the high expression of gga-miR-223 in our MDV-infected chickens. [score:7]
Linc-GALMD3 drives its cis and trans regulation in chicken MDA total of 751 target genes of gga-miR-223 were predicted by TargetScan 7.0 (see Supplementary Table  S4), of these, 27 genes were also identified as DEGs after loss of function of linc-GALMD3 (see Supplementary Table  S5). [score:6]
The target genes of gga-miR-223 was predicted using the online tools TargetScan 7.0 (http://www. [score:5]
Our study showed that the identified linc-GALMD3 positively regulated the expression of its downstream gga-miR-223 gene through its cis-regulation. [score:5]
Additionally, the expression of up/downstream neighboring genes of linc-GALMD3 were measured after linc-GALMD3 knockdown by shRNA3-1657, and the results showed that the gga-miR-223 expression was remarkably decreased after linc-GALMD3 knockdown. [score:5]
A total of 751 target genes of gga-miR-223 were predicted by TargetScan 7.0 (see Supplementary Table  S4), of these, 27 genes were also identified as DEGs after loss of function of linc-GALMD3 (see Supplementary Table  S5). [score:5]
In addition, a report showed that the serum miR-223 might be a potential biomarker for Alzheimer’s disease evaluation [59], and another study represented that miR-223/nuclear factor I-A axis could regulate glial precursor proliferation and tumorigenesis in the central nervous system [60], indicating that miR-223 might play critical roles in neurological diseases. [score:4]
Linc-GALMD3 activates gga-miR-223 expressionA rash of recent reports reveals that lncRNAs are powerful cis- and tran-regulators of gene activity 41, 42. [score:4]
Supplementary files Supplementary Table S3 748 DEGs Supplementary Table S4 Target genes of gga-miR-223 Supplementary Table S6 GO and KEGG pathway analysis This work was financially supported by the National Natural Science Foundation of China (31320103905), and the Programs for Changjiang Scholars and Innovative Research Team in University (IRT_15R62). [score:3]
Otherwise, gga-miR-223 was found highly expressed in lungs of avian influenza virus infected broilers [57]. [score:3]
Linc-GALMD3 activates gga-miR-223 expression. [score:3]
Yao et al. investigated miRNA expression profiles using microarray, and found that several miRNAs, including gga-miR-223, were down-regulated in all MDV-transformed cell lines compared to normal splenocytes [56]. [score:3]
Therefore, we considered that gga-miR-223 with its target DEGs might be associated with neurologic lesions in MD. [score:2]
Shi L MicroRNA-223 antagonizes angiogenesis by targeting beta1 integrin and preventing growth factor signaling in endothelial cellsCirculation research 113, 1320-1330 2013 50. [score:2]
In chickens, gga-miR-223 have been shown to be involved in the immune organs development [53], haemopoietic cell proliferation [54], and macrophage differentiation [55]. [score:2]
The black bar represented linc-GALMD3, NSGALT00000007468 and NSGALT00000007459 were the transcripts of upstream and downstream coding genes of linc-GALMD3, and NSGALT000000290030 was the transcript of the downstream gga-miR-223 gene. [score:1]
The linc-GALMD3 was located between two protein-coding genes, ENSGALG00000004688 and ENSGALG00000004681, in chicken chromosome 4 (Galgal3), and ENSGALT00000029030 was a transcript of gga-mir-223 gene located at downstream of linc-GALMD3. [score:1]
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[+] score: 31
For instance, gga-miR-34c has six targets in the present network, including SOCS3, GCG, APP, THY1, COL11A1 and MYH7B; gga-miR-146b-3p was predicted to target GHR and AKT1; gga-miR-223 was predicted to target FOXO3 and ADAM17; and the predicted target gene of gga-miR-9-5p was TGFBR2. [score:9]
McGirt L. Y. Adams C. M. Baerenwald D. A. Zwerner J. P. Zic J. A. Eischen C. M. miR-223 regulates cell growth and targets proto-oncogenes in mycosis fungoides/cutaneous T-cell lymphoma J. Investig. [score:4]
miR-223 was also found to regulate the cell proliferation by decreasing the FOXO1 gene expression in human [63]. [score:4]
Wu L. Li H. Jia C. Y. Cheng W. Yu M. Peng M. Zhu Y. Zhao Q. Dong Y. W. Shao K. MicroRNA-223 regulates FOXO1 expression and cell proliferation FEBS Lett. [score:3]
To verify the RNA-Seq data, the differential expression of four miRNAs including miR-223, miR-16, miR-205a and miR-222b-5p were validated by qRT-PCR among all four comparisons (Figure 4). [score:3]
miR-223 and miR-142-5p were found to potentially target the FOXO3 gene in our study. [score:3]
Shi L. Fisslthaler B. Zippel N. Frömel T. Hu J. Elgheznawy A. Heide H. Popp R. Fleming I. MicroRNA-223 antagonizes angiogenesis by targeting β1 integrin and preventing growth factor signaling in endothelial cells Circ. [score:2]
We found that many miRNAs have various isoforms in chicken breast muscle libraries, and some miRNAs have more than one highly abundant isoform (e. g., gga-let-7c, gga-miR-205a and gga-miR-223). [score:1]
Haneklaus M. Gerlic M. OʼNeill L. A. Masters S. L. miR-223: Infection, inflammation and cancer J. Intern. [score:1]
miR-223 was reported to associate with cell growth and proliferation, many signal pathways and various tumors [59, 60, 61]. [score:1]
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[+] score: 18
The two miRNAs that are significantly downregulated in IAH30 cells compared to HD11 –gga-miR-142-3p and gga-miR-223 (Figure 4B) have been shown to be involved in haemopoietic cell proliferation (Sun et al., 2010) and macrophage differentiation (Ismail et al., 2013). [score:3]
Similarly, the high expression levels of gga-miR-142-3p and gga-miR-223 in HD11 cells was also confirmed by quantitative RT-PCR (Figure 4B). [score:3]
Validation of expression levels of gga-miR-21, gga-miR-26a, gga-miR142-3p, gga-miR-155 and gga-miR-223 was carried out by quantitative RT-PCR using procedures described (Yao et al., 2008). [score:3]
Other miRNAs which are expressed at high levels in HD11 include gga-miR-142-3p (10.5%), gga-miR-223 (6.9%), gga-miR-19b (4%), gga-miR-20a (3.7%) and gga-miR-22 (3.4%). [score:3]
miR-223 and miR-142 attenuate hematopoietic cell proliferation, and miR-223 positively regulates miR-142 through LMO2 isoforms and CEBP-beta. [score:2]
Interestingly, gga-miR-142-3p and gga-miR-223 were significantly downregulated in IAH30 cells compared to HD11 cells Figure 4 For gga-miR-142-3p, there are only 19 reads in IAH30 compared to 75,670 reads in HD11. [score:2]
Similarly, there are 10 and 50,275 reads representing gga-miR-223 in IAH30 and HD11 respectively. [score:1]
Macrophage microvesicles induce macrophage differentiation and miR-223 transfer. [score:1]
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5
[+] score: 16
A recent study indicated that miR-223 was significantly up-regulated in the serum of mice infected with S. japonicum and returned to near normal levels on praziquantel treatment (He et al., 2013), implying that up-regulated murine miR-223 may be a biomarker for Schistosoma infection. [score:7]
In a recent study, miR-223 was primarily found in Kupffer cells in the liver, and the expression of this miRNA was dramatically elevated in liver cells of schistosome-infected mice (He et al., 2013); it is possible that the expression levels of miR-223 could reflect the extent of pathological changes in the liver of an infected host. [score:5]
However, the possibility that altered serum levels of miR-223 are caused by other diseases or the pathogenesis of other organs needs to be assessed. [score:3]
Host serum miR-223 is a potential new biomarker for Schistosoma japonicum infection and the response to chemotherapy. [score:1]
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6
[+] score: 8
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-16-1, hsa-mir-17, hsa-mir-18a, hsa-mir-19a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-20a, hsa-mir-21, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-106a, hsa-mir-16-2, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181a-1, hsa-mir-221, hsa-mir-222, hsa-mir-223, hsa-let-7g, hsa-let-7i, hsa-mir-15b, hsa-mir-23b, hsa-mir-27b, hsa-mir-122, hsa-mir-125b-1, hsa-mir-140, hsa-mir-125b-2, hsa-mir-136, hsa-mir-146a, hsa-mir-150, hsa-mir-206, hsa-mir-155, hsa-mir-181b-2, hsa-mir-106b, hsa-mir-302a, hsa-mir-34b, hsa-mir-34c, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-367, gga-let-7i, gga-let-7a-3, gga-let-7b, gga-let-7c, gga-mir-125b-2, gga-mir-155, gga-mir-222a, gga-mir-221, gga-mir-92-1, gga-mir-19b, gga-mir-20a, gga-mir-19a, gga-mir-18a, gga-mir-17, gga-mir-16-1, gga-mir-15a, gga-mir-1a-2, gga-mir-206, gga-mir-106, gga-mir-302a, gga-mir-181a-1, gga-mir-181b-1, gga-mir-16-2, gga-mir-15b, gga-mir-140, gga-let-7g, gga-let-7d, gga-let-7f, gga-let-7a-1, gga-mir-146a, gga-mir-181b-2, gga-mir-181a-2, gga-mir-1a-1, gga-mir-1b, gga-let-7a-2, gga-mir-34b, gga-mir-34c, gga-let-7j, gga-let-7k, gga-mir-23b, gga-mir-27b, gga-mir-24, gga-mir-122-1, gga-mir-122-2, hsa-mir-429, hsa-mir-449a, hsa-mir-146b, hsa-mir-507, hsa-mir-455, hsa-mir-92b, hsa-mir-449b, gga-mir-146b, gga-mir-302b, gga-mir-302c, gga-mir-302d, gga-mir-455, gga-mir-367, gga-mir-429, gga-mir-449a, hsa-mir-449c, gga-mir-21, gga-mir-1458, gga-mir-1576, gga-mir-1612, gga-mir-1636, gga-mir-449c, gga-mir-1711, gga-mir-1729, gga-mir-1798, gga-mir-122b, gga-mir-1811, gga-mir-146c, gga-mir-15c, gga-mir-449b, gga-mir-222b, gga-mir-92-2, gga-mir-125b-1, gga-mir-449d, gga-let-7l-1, gga-let-7l-2, gga-mir-122b-1, gga-mir-122b-2
In the present study, miR-223 was not significantly regulated while miR-181a was down-regulated in both infected lungs and tracheae. [score:5]
Selective expressions of miR-181a in the thymus and miR-223 in the bone marrow have been shown to be involved in the differentiation of pluripotent hematopoietic stem cells into the various blood cells lineages including B and T cells [40, 41]. [score:3]
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[+] score: 7
In this study, we found gga-miR-223 was up-regulated by chGH, indicating it might play a role in lipid metabolism in chicken liver. [score:4]
miR-223 is expressed higher in the liver of Large White pig (lean type) than in the liver of Tongcheng pig (fatty type) [44]. [score:3]
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8
[+] score: 7
Their study showed that several host-encoded miRNAs, including gga-miR-155, gga-miR-150, gga-miR-451, gga-miR-26a, and gga-miR-223, were down-regulated in all MDV-transformed cell lines compared to normal splenocytes, and nine MDV-1-encoded miRNAs were up-regulated in all of the MDV-transformed cell lines compared to the MDV -negative reticuloendotheliosis virus (REV)-T-transformed cell line AVOL-1. Previous studies have mainly focused on miRNA profiles of MDV-infected CEF and MDV-transformed cell lines and expression of several individual miRNAs in MDV -induced splenic tumors. [score:7]
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9
[+] score: 6
Other miRNAs from this paper: gga-mir-26a, gga-mir-146a, gga-mir-126, gga-mir-451
Comparative miRNA expression profiles of 7 MDV-transformed T-cell lines showed that host-encoded miRNAs such as miR-26a, miR-223, miR-150, miR-451 and miR-126 were consistently downregulated [3]. [score:6]
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10
[+] score: 6
Zardo G Polycombs and microRNA-223 regulate human granulopoiesis by transcriptional control of target gene expressionBlood. [score:6]
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11
[+] score: 5
Other miRNAs from this paper: hsa-mir-223
Likewise, if the constitutive expression of CSF2RA from the CAG promoter turns out problematic e. g. in pluripotent cells or early precursors, transgene expression may be restricted to the myeloid lineage utilizing myeloid-specific promoters, including the MRP8 [51] or miR223 [52] promoter. [score:5]
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12
[+] score: 4
The host miRNAs downregulated in the MDV-transformed cell lines include miR-155, miR-223, miR-150, miR-451, and miR-26a. [score:4]
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13
[+] score: 4
MiR-223 inhibits cholesterol biosynthesis by decreasing 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1) and methylsterol monooxygenase (MSMO1) expression [40]. [score:4]
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14
[+] score: 2
Further, miR-16 and miR-223 are involved in muscle cell development in chicken [64]. [score:2]
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[+] score: 1
Of these, nine (gga-mir-1b, gga-mir-7, gga-mir-7b, gga-mir-10b, gga-mir-31, gga-mir-130b, gga-mir-204, gga-mir-215, gga-mir-489) are increased, and five (gga-mir-223, gga-mir-124b, gga-mir-140, gga-mir-183, gga-mir-222a) are decreased in CD30 [hi] cells. [score:1]
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Moreover, the activation of TLR signals can also induce microRNAs (such as miR-146, miR-155 and miR-223) that can feedback the components in the TLR signalling system[9, 10]. [score:1]
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One module in the network contained 3 miRNAs (miR-223, miR-6670-5p and miR-190-3p) and one lncRNA (LOC_013679) interacting with 14 protein-coding genes, including Lef1 and TAP1. [score:1]
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