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22 publications mentioning gga-mir-30e

Open access articles that are associated with the species Gallus gallus and mention the gene name mir-30e. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

[+] score: 56
Another approach to multiple siRNA expression was stimulated by report that a mouse miR30 -based shRNA expression cassette can be driven by Pol II promoters and provide higher knockdown efficiency than those driven by the Pol III U6 promoter [10]. [score:6]
These results suggest that although NP miRNA can be expressed from the mouse miR30 -based cassette in DF-1 cells, the level of target gene knockdown is modest following stable integration of the lentiviral vector. [score:6]
Subsequently, Sun et al showed that a single Pol II promoter can drive three artificial miR30 cassettes to express siRNAs all targeting GFP, resulting in further knockdown of the GFP intensity in the cells [17]. [score:6]
The mouse miR30 -based miRNA expression cassette has been wi dely used to express artificial miRNA in lentiviral vectors [21]. [score:5]
As shown in Figure 1c, transient expression of miR30-NP inhibited Renilla luciferase activity by ∼85%. [score:5]
Inhibition of luciferase activity by NP miRNA expressed from a mouse miR30 -based lentiviral vector. [score:5]
To express anti-influenza artificial miRNA, we replaced the mature miR30 sequences in pLB2 with sequences that target nucleoprotein (NP) of influenza virus (Figure 1b). [score:5]
As a control, Vero cells were transduced with a CPGM lentivirus that expressed miR30 -based miRNA specific for the firefly luciferase transcript. [score:3]
Expression of NP miRNA from the mouse miR30 -based lentiviral vector. [score:3]
Zhou et al reported that two tandem copies of the miR30 -based cassette can be expressed in a single transcript driven by a Pol II promoter [15], [16]. [score:3]
In addition to miR30 -based designs, mouse miR155 -based design has also been used to knockdown multiple genes [19]. [score:2]
In the transient transfection assay, the miR30-NP lentiviral vector and psicheck-2 dual luciferase reporter plasmid, in which the NP target sequence was cloned into the 3′ UTR of the synthetic Renilla luciferase gene, were co -transfected into DF-1 cells. [score:2]
A similar miR30 -based approach was utilized by Zhu et al to knockdown multiple genes [18]. [score:2]
Flanking and hairpin sequences are miR30. [score:1]
0022437.g001 Figure 1(a) Schematic diagram of the miR30-NP lentiviral vector. [score:1]
Psicheck-2 dual luciferase reporter plasmid (50 ng) and miR30-NP lentiviral vector (450 ng) were co -transfected in DF-1 cells. [score:1]
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[+] score: 19
Other miRNAs from this paper: hsa-let-7d, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-21, hsa-mir-22, hsa-mir-30a, hsa-mir-32, hsa-mir-33a, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-147a, hsa-mir-34a, hsa-mir-187, hsa-mir-204, hsa-mir-205, hsa-mir-200b, hsa-mir-23b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-138-2, hsa-mir-142, hsa-mir-144, hsa-mir-125b-2, hsa-mir-138-1, hsa-mir-146a, hsa-mir-190a, hsa-mir-200c, hsa-mir-155, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-30e, hsa-mir-365b, hsa-mir-328, gga-mir-33-1, gga-mir-125b-2, gga-mir-155, gga-mir-17, gga-mir-148a, gga-mir-138-1, gga-mir-187, gga-mir-32, gga-mir-30d, gga-mir-30b, gga-mir-30a, gga-mir-30c-2, gga-mir-190a, gga-mir-204-2, gga-mir-138-2, gga-let-7d, gga-let-7f, gga-mir-146a, gga-mir-205b, gga-mir-200a, gga-mir-200b, gga-mir-34a, gga-mir-30c-1, gga-mir-205a, gga-mir-204-1, gga-mir-23b, gga-mir-142, hsa-mir-449a, hsa-mir-489, hsa-mir-146b, hsa-mir-548a-1, hsa-mir-548a-2, hsa-mir-548a-3, hsa-mir-33b, hsa-mir-449b, gga-mir-146b, gga-mir-147, gga-mir-489, gga-mir-449a, hsa-mir-449c, gga-mir-21, gga-mir-144, gga-mir-460a, hsa-mir-147b, hsa-mir-190b, gga-mir-22, gga-mir-460b, gga-mir-1662, gga-mir-1684a, gga-mir-449c, gga-mir-146c, gga-mir-449b, gga-mir-2954, hsa-mir-548aa-1, hsa-mir-548aa-2, hsa-mir-548ab, hsa-mir-548ac, hsa-mir-548ad, hsa-mir-548ae-1, hsa-mir-548ae-2, hsa-mir-548ag-1, hsa-mir-548ag-2, hsa-mir-548ah, hsa-mir-548ai, hsa-mir-548aj-1, hsa-mir-548aj-2, hsa-mir-548ak, hsa-mir-548al, hsa-mir-548am, hsa-mir-548an, hsa-mir-548ao, hsa-mir-548ap, hsa-mir-548aq, hsa-mir-548ar, hsa-mir-548as, hsa-mir-548at, hsa-mir-548au, hsa-mir-548av, hsa-mir-548aw, hsa-mir-548ax, hsa-mir-548ay, hsa-mir-548az, gga-mir-365b, gga-mir-33-2, gga-mir-125b-1, gga-mir-190b, gga-mir-449d, gga-mir-205c
Likewise, from the miRNA-mRNA association, the under expressed genes LZTFL1, JAZF1, THBS2 and RPS14 were associated with microRNAs (miR-146b-5p, miR-1684a-3p, miR-460b-3p, miR-30e-5p, miR-33-5p, miR-148a-5p, miR-32-5p, miR-155 and miR-144-3p) that were down-regulated in pulmonary arteries (Figure 4). [score:6]
From the current association analysis data, we observed that both differentially expressed miR-30e-3p and miR-125b-5p were associated with overexpression of CD44 in the pulmonary arteries of AS broilers. [score:5]
And Caruso et al firstly reported miRNAs dysregulation in the progress of PAH by the means of microarray analysis and quantitative polymerase chain reaction, who detected that miR-451and miR-322 up regulated, whereas miR-30, miR-22, and let-7f down regulated in PAH rodent mo dels [27]. [score:4]
We also identified five other microRNAs including miR-489-3p, miR-1662, miR-460b-5p, miR-144-3p and miR-30e-5p, which were involved in the regulation of HIF1α in these data. [score:2]
To validate the present results of miRNA-sequencing, miR-30E-3P, miR-187-3P, miR-1662, and miR-147 these four miRNAs were selected from all miRNAs identified in this study for qRT-PCR validation. [score:1]
And in this study, we found more than one miRNA, including miR-142-5p, miR-32-5p, miR-1662, miR-155, miR-30e-5p and miR-34a-5p, that function together to target LRP1B in the pulmonary arteries of AS broilers, which was characterized by vascular cells proliferation. [score:1]
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[+] score: 15
The expression level of miR-218-5p, miR-30-5p and miR-19-3p was downregulated by estrogen. [score:6]
As shown in Figure 3A, miR-218-5p, miR-30a-5p, miR-30b-5p, miR-30e-5p, miR-19a-3p, and miR-19b-3p were significantly downregulated in 17 β-estradiol treated groups (p < 0.01). [score:4]
Three miRNA clusters (miR-218-5p, miR-19-3p, and miR-30-5p) from 46 differentially expressed miRNA were found to potentially bind to the 3′UTR of ELOVL5 in the present study (Figure 2B). [score:3]
Among them, we found the seed sequences of miR-218-5p, miR-19a-3p, miR-19b-3p, miR-30a-5p, miR-30b-5p, miR-30d-5p, and miR-30e-5p were complementary with the 3′UTR of ELOVL5 (Figure 3A). [score:1]
Two miRNA (miR-124a-3p and miR-124b) and four miRNA clusters (miR-19-3p, miR-218-5p, miR-124-3p, and miR-30-5p) shared by three databases were found to be potentially combine the 3′UTR of ELOVL5, respectively (Figure 2A,B and Figure S3). [score:1]
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[+] score: 14
Among these 21 miRNAs, the gga-miR-30a and gga-miR-30e were highly expressed and reported to regulate the liver development [14], which indicated the roles of gga-miR-30a and gga-miR-30e in the regulation of postnatal liver maturation by targeting ESRP2. [score:8]
Moreover, we also found that miRNA-30 family and let-7 family were significantly differentially expressed between the postnatal and matured livers and were abundant in the liver, whose important roles in the liver development have been proved 14, 22. [score:4]
Hand NJ The microRNA-30 family is required for vertebrate hepatobiliary developmentGastroenterology. [score:2]
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[+] score: 12
Of the seventeen miRNAs that are expressed at higher levels at the later stage of development (HH25) in the chicken, duck, and quail (Table S7) four belong to the miR-30 family (miR-30a-3p, miR-30a-5p, miR-30d*, and miR-30e*). [score:4]
Taken together, these miRNA changes, including differential expression of let-7, miR-302, and miR-30 families (Table S7), indicate that the HH20 to HH25 developmental window may be a critical transition phase in which multipotent NC cells begin to differentiate to form the various tissues of the face. [score:4]
Thus, up-regulation of the miR-30 family might reflect an increase in cellular differentiation at HH25. [score:4]
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[+] score: 10
For example, a comparison of miRNA expression profiles in proliferating myoblasts and differentiated myotubes revealed that miR-221 and miR-222 are down-regulated upon differentiation of primary and established myogenic cells, whereas miR-21, miR-103, miR-130, miR-99, miR-30 and miR20 are up-regulated [19, 33], suggesting that these miRNAs play important roles in the transition between proliferation and differentiation of muscle cells. [score:9]
In addition to miR-206, miR-1 and miR-181, nine other miRNAs among the most abundant in these libraries (miR-221, miR-222, miR-21, miR-103, miR-130, miR-99, miR-30, miR20, and miR128) have been implicated in the proliferation and differentiation of muscle cells (Table 1) [15, 19, 33]. [score:1]
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[+] score: 8
Other down-regulated miRNAs include members of the miR-30 family of miRNAs (gga-miR-30c, gga-miR-30d, gga-miR-30a-5p) many of which have been implicated in a wide range of cancers (Gaziel-Sovran et al., 2011; Baraniskin et al., 2012; Cheng et al., 2012). [score:4]
Other downregulated miRNAs included members of the miR-30 family of miRNAs (gga-miR-30c, gga-miR-30d, gga-miR-30a-5p) and the avian-specific gga-miR-1729 originally discovered in developing chick embryo. [score:4]
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[+] score: 8
Of the top 20 abundantly expressed miRNAs identified, some, such as miR-133, miR-10b, miR-26a, miR-30e and gga-miR-30a were also abundantly expressed in chicken somites [41]. [score:5]
miRNAs Normalized Reads Total Reads WRRh WRRl XHh XHl gga-miR-133a 3,558,683 3,069,071 1,997,286 2,607,787 11,232,827 gga-miR-133c 3,350,936 2,885,440 1,878,925 2,449,209 10,564,510 gga-miR-133b 3,326,848 2,864,578 1,864,721 2,431,274 10,487,421 gga-let-7a 1,699,621 1,513,865 857,210 1,133,532 5,204,228 gga-miR-22-3p 1,333,233 1,145,421 712,464 988,186 4,179,304 gga-miR-30a-5p 1,213,468 1,148,128 790,893 930,507 4,082,996 gga-miR-26a 1,212,635 1,054,689 691,456 1,006,522 3,965,302 gga-miR-30d 851,887 813,262 583,932 667,002 2,916,083 gga-miR-181a-6p 918,452 836,452 485,661 650,836 2,891,401 gga-miR-10a-5p 943,686 782,180 420,809 663,401 2,810,076 gga-miR-10b 911,725 757,564 398,852 633,567 2,701,708 gga-miR-30e 799,679 730,832 501,718 596,218 2,628,447 gga-let-7j 848,972 756,205 428,182 566,165 2,599,524 gga-let-7f 398,292 363,598 206,995 274,333 1,243,218 gga-miR-148a 288,585 300,432 144,015 180,973 914,005 gga-miR-146c-5p 224,147 207,782 171,443 132,712 736,084 gga-let-7k 211,853 206,518 118,297 155,412 692,080 gga-let-7c 242,661 189,820 111,118 139,257 682,856 gga-miR-199-3p 168,417 152,158 75,346 121,460 517,381 gga-miR-126-5p 139,914 109,805 89,607 86,681 426,007 Differentially expressed miRNAs were identified by DEGseq analysis (fold change > 1.5 or < 0.66; p-value < 0.05; q-value < 0.01), as a result, 200, 279, 257 and 297 miRNAs were detected in four comparisons of WRRh vs. [score:3]
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[+] score: 6
In addition, the putative target genes of miR-30 family members (miR-30a, -30b, -30d, -30e, and -30c) encode proteins involved in glycerophospholipid metabolism, biosynthesis of unsaturated fatty acids, pantothenate and CoA biosynthesis, and PPAR signalling pathway. [score:3]
Members of the miR-30 family are important positive regulators of adipocyte differentiation in a human adipose tissue-derived stem cell mo del 44. [score:2]
Seven conserved families all were DE with P ≤ 0.05, including let-7 (let-7a, -7b, -7c,-7f, -7g, -7i, -7j, and -7k), miR-130 (miR-130a, and -130b), miR-146 (miR-146a, -146b, and -146c), miR-15 (miR-15a, -15b, and -15c), miR-181 (miR-181a and -181b), miR-29 (miR-29a, -29b and -29c), and miR-30 (miR-30a, -30b, -30c, -30d, and -30e). [score:1]
[1 to 20 of 3 sentences]
[+] score: 6
3.4Wang et al. identified 32 known miRNAs from skeletal muscle of Arbor Acres commercial chickens, of which 12 form five clusters: miR-133a-1-miR-1a-2, miR-23b-miR-24, miR-99a-let-7c, miR-92-miR-19b-miR-18a-miR-17, and miR-30e-miR-30c-1, suggesting that most miRNAs co-express in skeletal muscle [85]. [score:3]
Wang et al. identified 32 known miRNAs from skeletal muscle of Arbor Acres commercial chickens, of which 12 form five clusters: miR-133a-1-miR-1a-2, miR-23b-miR-24, miR-99a-let-7c, miR-92-miR-19b-miR-18a-miR-17, and miR-30e-miR-30c-1, suggesting that most miRNAs co-express in skeletal muscle [85]. [score:3]
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[+] score: 4
Members of the miR-30 family function in osteoblast differentiation, adipogenic differentiation, angiogenesis, myocardial matrix remo deling and cell apoptosis by targeting various pathways [18– 22, 33]. [score:3]
Gga-miR-30b and gga-miR-30c share the same seed regions and belong to the gga-miR-30 family. [score:1]
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[+] score: 4
The following 15 miRNAs were dominantly expressed in the two libraries: gga-miR-10a, gga-miR-146c, gga-miR-101, gga-miR-21, gga-let-7a, gga-let-7b, gga-let-7c, gga-let-7j, gga-let-7f, gga-let-7 k, gga-miR-30a-5p, gga-miR-30e, gga-miR-148a, gga-miR-100 and gga-miR-126. [score:3]
Similarly, other miRNA families exhibited vast read ranges, such as the gga-miR146 (91–235,844 reads) and gga-miR-30 (886–164,361 reads) families (Additional file 1: Table S2). [score:1]
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[+] score: 4
Eleven of these (miR-204, miR-19a-3p, miR-19b-3p, miR-30d, miR-26a, miR-122-5p, miR-103-3p, miR-27b-3p, miR-92-3p, miR-142-3p, and miR-17-5p) have been implicated, directly or indirectly, in fat deposition; 9 showed a high fold-change (miR-3535, miR-144-3p, miR-30e-5p, miR-301b-3p, miR-215-5p, miR-200a-3p, miR-133a-3p, miR-133c-3p, and miR-146b-5p). [score:3]
Potentially novel miRNAs (gga-miR-3535, miR-30e-5p, miR-301b-3p, miR-215-5p, miR-200a-3p, miR-133a-3p, miR-133c-3p, and miR-146b-5p) and genes (LAMA2, RAP1B, PECR, AKT1, ITGALL and CHAD) related to abdominal adipose tissue were also identified. [score:1]
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[+] score: 4
The present study of splenic miRNA and mRNA profiles from chickens after Salmonella challenge has identified differential expression of several miRNAs linked to immune responses, including miR-155, miR-9, miR-30 which have been reported previously and several miRNAs, such as miR-101-3p and miR-130b-3p, which were shown here to be associated with the immune response to infection with SE. [score:3]
Several miRNAs previously reported to be involved in immune responses such as miR-155, miR-9, miR-30, miR-126, and miR-29 families were identified. [score:1]
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[+] score: 3
Most of the changed microRNAs are involved in development (especially in the embryonic development) and some examples of them are miRNA-222, miRNA-383, miRNA-126, miRNA-133, miRNA-30, miRNA-10a, miRNA-196 and miRNA-18b. [score:3]
[1 to 20 of 1 sentences]
[+] score: 2
The mir-30 family of miRNAs have been shown to be involved in early muscle development in zebrafish [18] and the mouse equivalent (mmu-mir-100-5p) of gga-mir-100-5p is involved in embryonic stem-cell differentiation [19]. [score:2]
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[+] score: 2
Other miRNAs from this paper: gga-mir-30d, gga-mir-30b, gga-mir-30a, gga-mir-30c-2, gga-mir-30c-1
Following PCR to form the siRNA with hairpin primers, the siRNA PCR fragment was then cloned into the miRNA30-like hairpin insertion site in a slx12 shuttle vector containing RFP, along with a U6 siRNA cassette containing the chicken U6 promoter and a microRNA operon expression cassette. [score:2]
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[+] score: 2
Other miRNAs from this paper: gga-mir-30d, gga-mir-30b, gga-mir-30a, gga-mir-30c-2, gga-mir-30c-1
Gene-specific shRNAs are embedded into the primary transcript of human miR30, which is located in the 3′UTR of a doxycycline-regulated GFP transgene. [score:2]
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[+] score: 1
In addition to these transcripts our data list microRNAs 490-5p&3p, 1677-5p, 126-5p, 1434-5p, 193b-3p, and the members of the miR30 family, i. e., 30a-5p, 30b-5p, 30d-5p and 30e-5p, as novel cardiac enriched transcripts. [score:1]
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[+] score: 1
A number of miRNA backbones can be used, including miR-155, miR-30 and miR17-92 [23- 25]. [score:1]
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[+] score: 1
Further, deep sequencing detected let-7, miR-21, and miR-30 in rainbow trout eggs, which indicated that these miRNAs may play roles in controlling egg quality [21]. [score:1]
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[+] score: 1
Other miRNAs from this paper: gga-mir-30d, gga-mir-30b, gga-mir-30a, gga-mir-30c-2, gga-mir-30c-1
Sox14 specific oligonucleotides and a scrambled sequence were subsequently cloned in the second miRNA site of vector pRFPRNAi to generate synthetic miRNA30-like hairpins under the control of the chick U6 snRNA promoter, as described in [25]. [score:1]
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