2 publications mentioning aga-mir-10

Open access articles that are associated with the species Anopheles gambiae and mention the gene name mir-10. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

We identified bantam, miR-263a, miR-8, miR-10, miR-184 and miR-281 as the most abundantly expressed miRNAs in A. gambiae (Additional file 1: Figure S2).

We identified miR-10 and miR-210 with an extra 5’ cytosine matched to the sequence of the pre-miRNA.

Since in A. gambiae there are no paralogs of miR-10 and miR-210, this difference cannot be due to processing of mature miR from distinct homologous precursors.

We found that miR-125, miR-283, miR-10, miR-100, miR-281* and certain abundant isoforms of miR-279, miR-1174, miR-263a and miR-281 were substantially adenylated.

Similar variation at the 5’-end has been described in D. melanogaster miR-10 and miR-210, illustrating an example of the single hairpin generating mature miR with different abundant 5’-ends [34, 37].

Among the extremely abundant miRNAs, 1–2 nt trimming at the 3’-end was observed for 20% of reads from bantam and miR-10; for 17% of reads from miR-263a and for 32% of reads from miR-184 (Figure  3D).

Importantly, we identified a group of miRNAs, including miR-283, miR-2, miR-210, miR-263a, miR-10 and miR-252, with heterogenous 5’-ends (Figure  3C).

For miR-10, two dominant mature species occur with the originally annotated 5’-end (~87% of reads) and with an extra 5’ cytosine (~12% of reads) (Figure  3C).

2

In contrast, the cellular levels of miR-10-3p and miR-1174-3p decreased by >4-fold at 12 h PBM, but their association with Ago1 did not change accordingly.