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27 publications mentioning ssc-mir-148a

Open access articles that are associated with the species Sus scrofa and mention the gene name mir-148a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 40
A recent study has demonstrated that miR-148a expression is also upregulated in DCs on maturation and activation induced by TLR3, TLR4, and TLR9 agonists, which, in turn, inhibit the upregulation of MHC class II expression, the production of cytokines including IL-12, IL-6, TNF-alpha, and IFN-beta, and antigen presentation of DCs by directly targeting Calcium/calmodulin -dependent protein kinase II [91]. [score:16]
The upregulated miR-148a in PBMCs of H1N1 critically ill patients may contribute to the regulation of innate and adaptive immune responses. [score:5]
Moreover, TGFBR1 and TP53 were both predicted to be regulated by high-expressed miR-148a. [score:4]
validation of differentially expressed miRNAs and ROC analysisThe microarray data were validated by performing, qRT-PCR for nine miRNAs, including hsa-miR-146b-5p, hsa-miR-148a, hsa-miR-150, hsa-miR-31, hsa-miR-155, hsa-miR-29a, hsa-miR-29b, hsa-miR-342-5p, and hsa-miR-886-3p. [score:3]
The expression of hsa-miR-150, hsa-miR-31, hsa-miR-155, hsa-miR-29a, hsa-miR-29b, hsa-miR-342-5p, and hsa-miR-146b-5p were present in lower abundance, whereas hsa-miR-148a and hsa-miR-886-3p were present in higher abundance in PBMCs from critically ill patients infected with H1N1 influenza virus than that from healthy controls. [score:3]
Their result indicates that miR-148a is a negative regulator of the innate response and antigen presenting capacity of DCs. [score:2]
We found that miR-148a was significantly upregulated compared with the control samples by qRT-PCR assay, indicating that miR-148a has an important function in influenza virus infection. [score:2]
MiR-148a has been associated with different types of cancer [87, 88] and autoimmune diseases, such as multiple sclerosis [23], asthma [89] and systemic lupus erythematosus [90]. [score:2]
ROC curve analyses revealed that miR-31, miR-29a and miR-148a all had significant potential diagnostic value for critically ill patients infected with H1N1 influenza virus, which yielded AUC of 0.9510, 0.8951 and 0.8811, respectively. [score:1]
The microarray data were validated by performing, qRT-PCR for nine miRNAs, including hsa-miR-146b-5p, hsa-miR-148a, hsa-miR-150, hsa-miR-31, hsa-miR-155, hsa-miR-29a, hsa-miR-29b, hsa-miR-342-5p, and hsa-miR-886-3p. [score:1]
ROC curve analyses revealed that miR-31, miR-29a and miR-148a were valuable biomarkers for differentiating critically ill patients from controls: miR-31 yielded an AUC (the areas under the ROC curve) of 0.9510 (95% CI: 0.8734–1.029; P = 0.0001884) with 81.82% sensitivity and 92.31% specificity in discriminating critically ill patients; miR-29a yielded AUC of 0.8951 (95% CI: 0.7412–1.049 P = 0.0001070) with 90.91% sensitivity and 92.31% specificity in discriminating critically ill patients, and miR-148a yielded AUC of 0.8811 (95% CI: 0.7360–1.026 P = 0.001601) with 72.73% sensitivity and 100% specificity in discriminating critically ill patients(Figure 5). [score:1]
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2
[+] score: 39
Group 3: MicroRNAs down-regulated in control followed by up-regulation in visually unaffected and down-regulation again in necrotic sample (miR-142-5p, miR-143-3p, miR-148a). [score:10]
Unpublished time-course studies of mRNA from lung tissue reveal IL-6 first to be highly up-regulated and then to be significant down-regulated somewhere between 12 and 24 h after infection with APP (Skovgaard, personal communication) MicroRNA miR-148a and miR-126 are also mentioned in the inflammation related literature with miR-148a having implication in function of primary bronchial epithelial cells [70] and miR-126 in chronic asthma where initial increase in expression of this microRNA was found [71]. [score:9]
This corresponds to great up-regulation of miR-148a in infected tissue. [score:4]
MiR-142-5p, miR-148a and miR-451 target over five mRNAs, whereas only two predictions were found for miR-126. [score:3]
The miR-148a similarly to miR-146a-5p was found to target mRNA encoding IRAK1. [score:3]
Surprisingly the differential expression detected by RT-qPCR does not confirm the lack of significant differences for the miR-148a and miR-143 shown by RNAseq data. [score:3]
Despite the decreasing trend, the expression for miR-142-5p and miR-148a, in the necrotic sample remains significantly higher than in the control. [score:3]
Our most confident prediction for miR-148a is a SMAD2 gene involved in regulation of cell growth and proliferation - two processes which are rather silenced during stressful bacterial infection. [score:2]
The ncRNAs chosen for RT-qPCR validation were: miR-15a, miR-21, miR-126, miR-142-5p, miR-143-3p, miR-144*, miR-146a-5p, miR-148a, miR-155, miR-223, miR-451, miR-664-5p, miR-d5 and SNORD15. [score:1]
Of the miRNAs investigated in the present study, miR15a, miR21, miR126, miR142-5p, miR144-5p, miR146a-5p, miR148a, miR152, miR155, miR192, miR-223, miR-45 and miR-d5 are 5'-miRNAs hence only the mature miRNAs of these targets were detected. [score:1]
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3
[+] score: 29
MiR-9-5p, miR-148a and miR-125a also have target sites in SCD, which is upregulated in the adipose tissue of the obese minipigs. [score:6]
LEP has target sites for three miRNAs: MiR-30a, miR-148a and miR-9-5p which were all downregulated in obese adipose tissue and muscle. [score:6]
LEP was the gene containing the most miRNA target sites, i. e. is targeted by miR-148a-3p, miR-125a-5p, miR-30a, miR-9-5p and miR-17-5p. [score:5]
MiR-204, miR-148a, miR-30a, miR-196b, and miR-17a were downregulated with fold changes of < -1.5 and p values < 0.05. [score:4]
MiR-30a and miR-148 are both involved in adipocyte differentiation, downregulated in obese adipose tissue in mice and are involved in myogenic differentiation [37– 39]. [score:4]
SCD is also targeted by many of the same miRNAs, namely miR-148a-3p, miR-125a-5 and miR-9-5p. [score:3]
In addition, miR-30a, miR-125a and miR-148a all had fold changes of < -1.5 and p values < 0.05. [score:1]
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4
[+] score: 24
Several miRNAs expressed at a relatively low level in skeletal muscles included ssc-miR-143-3p, expressed mainly in the colon; ssc-miR-148a, expressed in the liver and colon; and ssc-miR-30a-5p, expressed at extremely high levels in the kidney. [score:9]
Zhang et al. revealed that miR-148a positively regulated myogenic differentiation by downregulation of ROCK1 recently [40]. [score:5]
In addition, we found that ssc-miR-148a was expressed highly in the longissimus muscles, while it was expressed in an extremely low level in their results. [score:5]
Moreover, other miRNAs were also expressed abundantly in skeletal muscles, including ssc-let-7a, ssc-let-7c, ssc-let-7f, ssc-miR-143-3p, ssc-miR-10b, ssc-miR-148a, ssc-miR-127, ssc-miR-30d, ssc-miR-30a-5p, and ssc-miR-181a. [score:3]
MiR-148a inhibits cell growth and attenuates migration and invasion in prostate cancer PC3 cells [39]. [score:2]
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5
[+] score: 24
This pattern included the two created target sites for ssc-miR-34a and ssc-miR-34c, both predicted by TargetScan and PACMIT in SLA-1 (Figure 3 A); the disrupted target site for ssc-miR-148a in HSPA1A predicted by PACMIT and TargetSpy (Figure 3 B); the ssc-miR-133b (TargetScan and PACMIT), ssc-miR-133a-3p (TargetScan) and ssc-miR-323 (TargetSpy) created target sites in RNF5 (Figure 3 C); and the disrupted site for ssc-miR-2320 predicted by TargetSpy in SLA-1 (Figure 3D). [score:19]
Allele-specific targeting of HLA-G, a non-classical HLA class I locus, by miR-148a and miR-148b, is associated with risk of asthma [41]. [score:3]
This can be explained by the additional accessibility criterion of PACMIT as sites considered as created or disrupted by PACMIT may only reveal changes in secondary structures which are not predicted by TargetScan (e. g. the created ssc-miR-339-5p and ssc-miR-4334-3p sites in CREBL1 3′-UTR, and the disrupted ssc-miR-148a, ssc-miR-148b and ssc-miR-152 sites in HSPA1A). [score:2]
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6
[+] score: 23
In this study, the results of integrated expression analysis showed that miR-143-3p, miR-30a-5p, miR-novel-chr2_21624, miR-148a-3p, miR-27b-3p and miR-10a-5p were significantly upregulated in PCMV-infected thymus, while the expression of their target genes, which are related to the TLR and RLR signalling pathways, were significantly downregulated (Table 2). [score:13]
67 Toll-like receptor signaling pathway miR-148a-3p +2.46 STAT5B −2.55 Toll-like receptor signaling pathway miR-148a-3p +2.46 DDX3X −2.94 RIG-I-like receptor signaling pathway miR-novel-chr2_21624 +6.68 IKBKB −2.16 Toll-like receptor signaling pathway miR-novel-chr2_21624 +6.68 MAP3K7 −3.96 Toll-like receptor signaling pathway miR-novel-chr2_21624 +6.68 MAP3K7 −3.96 RIG-I-like receptor signaling pathway miR-novel-chr2_21624 +6.68 MAPK14 −3.77 RIG-I-like receptor signaling pathway “+” and “–” indicate upregulated and downregulated miRNAs or mRNAs, respectively. [score:7]
control Log2 (Fold change value) Target gene related signaling pathways miR-10a-5p +2.53 TLR6 −2.88 Toll-like receptor signaling pathway miR-10a-5p +2.53 TLR7 −2.01 Toll-like receptor signaling pathway miR-27b-3p +4.84 PIK3CG −2.77 Toll-like receptor signaling pathway miR-27b-3p +4.84 JAK2 −2.15 Toll-like receptor signaling pathway miR-27b-3p +4.84 DHX58 −4.95 RIG-I-like receptor signaling pathway miR-30a-5p +3.99 CXCL9 −3.93 Toll-like receptor signaling pathway miR-30a-5p +3.99 IL12B −4.2 Toll-like receptor signaling pathway miR-30a-5p +3.99 IL12B −4.2 RIG-I-like receptor signaling pathway miR-30a-5p +3.99 MAP3K1 −3.03 RIG-I-like receptor signaling pathway miR-30a-5p +3.99 TANK −2.09 RIG-I-like receptor signaling pathway miR-143-3p +6.68 CD40 −4.97 Toll-like receptor signaling pathway miR-143-3p +6.68 JAK3 −2.69 Toll-like receptor signaling pathway miR-143-3p +6.68 DDX58 −4.44 RIG-I-like receptor signaling pathway miR-143-3p +6.68 IKBKB −2.16 RIG-I-like receptor signaling pathway miR-148a-3p +2.46 MAPK14 −3.77 Toll-like receptor signaling pathway miR-148a-3p +2.46 STAT1 −2. [score:3]
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7
[+] score: 23
This study used high-throughput sequencing to compare miRNA expression in pigs susceptible and resistant to E. coli F18 infection and identified 12 miRNAs with differential expression, including 11 upregulated in susceptible animals, ssc-miR-143, ssc-let-7f, ssc-miR-30e, ssc-miR-148a, ssc-miR-148b, ssc-miR-181a, ssc-miR-192, ssc-miR-27b, ssc-miR-15b, ssc-miR-21, ssc-miR-215, and one down-regulated, ssc-miR-152. [score:11]
These included 11 with increased miRNA expression in E. coli F18-sensitive pigs, ssc-miR-143, ssc-let-7f, ssc-miR-30e, ssc-miR-148a, ssc-miR-148b, ssc-miR-181a, ssc-miR-192, ssc-miR-27b, ssc-miR-15b, ssc-miR-21, ssc-miR-215, and one with reduced miRNA expression, ssc-miR-152. [score:5]
In another important human disease, diabetes, the study by Melkman et al. [5] into the effects of miRNAs on insulin synthesis revealed that knocking out miR-24, miR-26, miR-182, or miR-148 reduced the transcriptional activity of the insulin gene promoter, thereby reducing the level of insulin mRNA. [score:4]
Yue et al. found that miR-148a and miR-152 expression is reduced in gastrointestinal cancers compared with para-cancerous tissue [20]. [score:2]
l Note: 1, ssc-miR-27b; 2, ssc-miR-215; 3, ssc-miR-21; 4, ssc-miR-192; 5, ssc-miR-15b; 6, ssc-miR-148a; 7, ssc-miR-143–5p; 8, ssc-let-7f; 9, ssc-miR-152. [score:1]
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8
[+] score: 18
The unified set of top 10 unique miRNAs over the two pig breeds correspond to 15 unique miRNAs, 11 of which (ssc-let-7a-1/2-5p, ssc-let-7c-5p, ssc-let-7e-5p, ssc-miR-10a-5p, ssc-miR-10b-5p, ssc-miR-127-3p, ssc-miR-148a-3p, ssc-miR-199a-1/2-5p, ssc-miR-21-5p, ssc-miR-26a-5p, ssc-miR-125b-1-5p) had been frequently reported highly expressed in skeletal muscle during porcine prenatal and postnatal developmental stages. [score:4]
Study of Mai et al. revealed that ssc-let-7a-1/2-5p, ssc-miR-10a-5p, ssc-miR-127-3p, ssc-miR-148a-3p, ssc-miR-199a-1/2-5p, ssc-miR-26a-5p were the most highly expressed unique miRNAs over five porcine muscle developmental stages from 90 dpc to 7 y after birth [27]. [score:4]
Some myogenesis related miRNAs (miR-133, miR-1, miR-206 and miR-148a) are highly abundant in MS pigs, while other miRNAs (let-7 family, miR-214, miR-181) highly expressed in LW. [score:3]
Interestingly, Among them, miR-133, miR-1, miR-206 and miR-148a were highly abundant in MS pigs, while let-7 family, miR-214 and miR-181 were highly expressed in LW. [score:3]
MiR-148a mediated myogenic differentiation via targeting ROCK1 [43]. [score:2]
For example, Qin et al. had reported that ssc-let-7a, ssc-miR-10a, ssc-miR-10b, ssc-miR-127, ssc-miR-148a, ssc-miR-21, ssc-miR-26a were the most abundant miRNAs during porcine skeletal muscle developmental stages from 35 days post coitum to postnatal day 180 [25]. [score:2]
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9
[+] score: 18
Target prediction of the 29 differentially expressed miRNAs revealed that miR-148a-3p, miR-27b-3p, miR-423-5p, miR-125a, miR-181c, and miR-365-3p target TNF-α. [score:7]
The miR-148 family, including miR-148a and miR-148b, has been reported to be negative regulators of the innate immune response, which in turn inhibit the production of cytokines including TNF-α in dendritic cells (53). [score:4]
The downregulation of miR-148a-3p after TNF-α treatment in our microarray analysis was an interesting finding. [score:4]
Liu X., Zhan Z., Xu L., Ma F., Li D., Guo Z., Li N., and Cao X. 2010 MicroRNA-148/152 impair innate response and antigen presentation of TLR-triggered dendritic cells by targeting CaMKIIα. [score:2]
Potentially, miR-148a-3p also participates in immune activity in porcine adipocytes and forms a loop with TNF-α. [score:1]
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10
[+] score: 14
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-27a, hsa-mir-29a, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-199a-1, hsa-mir-208a, hsa-mir-148a, hsa-mir-10a, hsa-mir-181a-2, hsa-mir-181c, hsa-mir-199a-2, hsa-mir-181a-1, hsa-mir-214, hsa-mir-221, hsa-let-7g, hsa-let-7i, hsa-mir-1-2, hsa-mir-23b, hsa-mir-27b, hsa-mir-125b-1, hsa-mir-128-1, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-143, hsa-mir-125b-2, hsa-mir-126, hsa-mir-127, hsa-mir-206, hsa-mir-1-1, hsa-mir-128-2, hsa-mir-29c, hsa-mir-26a-2, hsa-mir-378a, hsa-mir-148b, hsa-mir-133b, hsa-mir-424, ssc-mir-125b-2, ssc-mir-23a, ssc-mir-24-1, ssc-mir-26a, ssc-mir-29b-1, ssc-mir-181c, ssc-mir-214, ssc-mir-27a, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-128-1, ssc-mir-29c, hsa-mir-486-1, hsa-mir-499a, hsa-mir-503, hsa-mir-411, hsa-mir-378d-2, hsa-mir-208b, hsa-mir-103b-1, hsa-mir-103b-2, ssc-mir-17, ssc-mir-221, ssc-mir-133a-1, ssc-mir-1, ssc-mir-503, ssc-mir-181a-1, ssc-mir-206, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-378-1, ssc-mir-133b, ssc-mir-29a, ssc-mir-199a-2, ssc-mir-128-2, ssc-mir-499, ssc-mir-143, ssc-mir-10a, ssc-mir-486-1, ssc-mir-103-2, ssc-mir-181a-2, ssc-mir-27b, ssc-mir-24-2, ssc-mir-23b, ssc-mir-148b, ssc-mir-208b, ssc-mir-424, ssc-mir-127, ssc-mir-125b-1, hsa-mir-378b, hsa-mir-378c, ssc-mir-411, ssc-mir-133a-2, ssc-mir-126, ssc-mir-199a-1, ssc-mir-378-2, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, hsa-mir-499b, ssc-let-7a-2, ssc-mir-486-2, hsa-mir-378j, ssc-let-7d, ssc-let-7f-2, ssc-mir-29b-2, hsa-mir-486-2, ssc-mir-378b
Similarly, ssc-miR-148b, -542-3p and -30 family (a-5p/d/e-5p) showed similar expression patterns with ssc-miR-148a and -126, highly expressed and down-regulated at 77 dpc to 180 dpn (Figure 5E), making it possible that they belong to the candidate myogenic miRNAs. [score:8]
MiR-148a has been identified as a novel myogenic miRNA that mediated myogenic differentiation via targeting ROCK1 [27], while miR-126 attenuated insulin signaling [57] and governed vascular integrity and angiogenesis [58], suggesting their interactions with signaling pathways were required for muscle normal development and maintenance. [score:4]
Another DE miRNA (miR-148a), whose average abundance before birth was eight times higher than that in postnatal, might be a part of mechanism implicated in differences between embryonic myogenesis and adult myofiber maturation. [score:1]
In addition to the best-studied myomiRs (miR-1, -206 and miR-133 families), 11 other DE muscle-related miRNAs (miR-378 [24], miR-148a [27], miR-26a [28, 29], miR-27a/b [30, 31], miR-23a [32, 33], miR-125b [34], miR-24 [35], miR-128 [36], miR-199a [37] and miR-424 [38]) with high abundance (average RPM >1,000) and another 14 (miR-181a/b/c/d-5p [26], miR-499-5p [11], miR-503 [38], miR-486 [39], miR-214 [40], miR-29a/b/c [41– 43], miR-221/222 [44] and miR-208 [11] with low abundance (average RPM <1,000) were detected in myogenesis of pig. [score:1]
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11
[+] score: 12
As presented in Table 2, one class showed 100-fold greater levels of expression at E90 compared to D100, which included ssc-miR-126, ssc-miR-143-3p, ssc-miR-127, ssc-miR-148a, ssc-miR-196b-5p, and ssc-miR-369; another class exhibited expression levels of were slightly lower than 100-fold, which included ssc-miR-542-3p, ssc-miR-99b, ssc-miR-378, ssc-miR-30a-5p, ssc-miR-10b, and ssc-miR-21. [score:4]
Nine differentially expressed miRNAs (ssc-miR-7a, ssc-miR-10b, ssc-miR-21, ssc-miR-30d, ssc-miR-127, ssc-miR-148a, ssc-miR-181, ssc-miR-199*, and ssc-miR-378) were chosen for the validation of the Solexa sequencing data via RT-qPCR. [score:3]
Notably, the expression levels of ssc-miR-127 and ssc-miR-148a were much higher in the skeletal muscle at E90 than at D100, indicating that they both play roles in the promotion of myogenesis. [score:3]
Aside from ssc-miR-206 and ssc-miR-1, ssc-miR-378 was the most abundant at E90, followed by ssc-miR-143-3p, ssc-let-7a, ssc-let-7f, ssc-let-7c, ssc-miR-30d, ssc-miR-30a-5p, ssc-miR-10b, ssc-miR-127, ssc-miR-148a, ssc-miR-126, ssc-miR-7i, and ssc-miR-21. [score:1]
Our group has found that miR-148a is a novel myogenic miRNA that promotes myogenic differentiation by repressing the ROCK1 gene [9]. [score:1]
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12
[+] score: 12
Moreover, 13 miRNAs were differentially expressed: 8 were upregulated (ssc-miR-132, ssc-miR-146b, ssc-miR-215, ssc-miR-371, ssc-miR-27a, ssc-miR-331-3p, ssc-miR-432-5p and ssc-miR-199a/b-3p), while 5 were down-regulated after PRV infection (ssc-mir-10a-5p, ssc-mir-148-3p, ssc-mir-219a, ssc-mir-374b-3p and ssc-miR-532-5p) (Fig 7). [score:9]
Expression of miR-148 is regarded as a potential biomarker in non-small-cell lung cancer [45]. [score:3]
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13
[+] score: 11
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-16-1, hsa-mir-21, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-29a, hsa-mir-30a, hsa-mir-31, hsa-mir-99a, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-16-2, hsa-mir-192, hsa-mir-148a, hsa-mir-10b, hsa-mir-181a-2, hsa-mir-181a-1, hsa-mir-215, hsa-mir-223, hsa-mir-224, hsa-mir-200b, hsa-mir-15b, hsa-mir-27b, hsa-mir-125b-1, hsa-mir-141, hsa-mir-143, hsa-mir-152, hsa-mir-125b-2, hsa-mir-126, hsa-mir-146a, hsa-mir-184, hsa-mir-200c, hsa-mir-155, hsa-mir-29c, hsa-mir-200a, hsa-mir-99b, hsa-mir-296, hsa-mir-30e, hsa-mir-26a-2, hsa-mir-378a, hsa-mir-342, hsa-mir-148b, hsa-mir-451a, ssc-mir-125b-2, ssc-mir-15b, ssc-mir-184, ssc-mir-224, ssc-mir-23a, ssc-mir-24-1, ssc-mir-26a, ssc-mir-29b-1, ssc-let-7f-1, ssc-mir-103-1, ssc-mir-21, ssc-mir-29c, hsa-mir-486-1, hsa-mir-499a, hsa-mir-671, hsa-mir-378d-2, bta-mir-26a-2, bta-mir-29a, bta-let-7f-2, bta-mir-103-1, bta-mir-148a, bta-mir-16b, bta-mir-21, bta-mir-499, bta-mir-99a, bta-mir-125b-1, bta-mir-126, bta-mir-181a-2, bta-mir-27b, bta-mir-31, bta-mir-15b, bta-mir-215, bta-mir-30e, bta-mir-148b, bta-mir-192, bta-mir-200a, bta-mir-200c, bta-mir-23a, bta-mir-29b-2, bta-mir-29c, bta-mir-10b, bta-mir-24-2, bta-mir-30a, bta-mir-200b, bta-let-7a-1, bta-mir-342, bta-let-7f-1, bta-let-7a-2, bta-let-7a-3, bta-mir-103-2, bta-mir-125b-2, bta-mir-15a, bta-mir-99b, hsa-mir-664a, ssc-mir-99b, hsa-mir-103b-1, hsa-mir-103b-2, ssc-mir-15a, ssc-mir-16-2, ssc-mir-16-1, bta-mir-141, bta-mir-143, bta-mir-146a, bta-mir-152, bta-mir-155, bta-mir-16a, bta-mir-184, bta-mir-24-1, bta-mir-223, bta-mir-224, bta-mir-26a-1, bta-mir-296, bta-mir-29d, bta-mir-378-1, bta-mir-451, bta-mir-486, bta-mir-671, bta-mir-29e, bta-mir-29b-1, bta-mir-181a-1, ssc-mir-181a-1, ssc-mir-215, ssc-mir-30a, bta-mir-2318, bta-mir-2339, bta-mir-2430, bta-mir-664a, bta-mir-378-2, ssc-let-7a-1, ssc-mir-378-1, ssc-mir-29a, ssc-mir-30e, ssc-mir-499, ssc-mir-143, ssc-mir-10b, ssc-mir-486-1, ssc-mir-152, ssc-mir-103-2, ssc-mir-181a-2, ssc-mir-27b, ssc-mir-24-2, ssc-mir-99a, ssc-mir-148b, ssc-mir-664, ssc-mir-192, ssc-mir-342, ssc-mir-125b-1, oar-mir-21, oar-mir-29a, oar-mir-125b, oar-mir-181a-1, hsa-mir-378b, hsa-mir-378c, ssc-mir-296, ssc-mir-155, ssc-mir-146a, bta-mir-148c, ssc-mir-126, ssc-mir-378-2, ssc-mir-451, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, hsa-mir-451b, hsa-mir-499b, ssc-let-7a-2, ssc-mir-486-2, hsa-mir-664b, hsa-mir-378j, ssc-let-7f-2, ssc-mir-29b-2, ssc-mir-31, ssc-mir-671, bta-mir-378b, bta-mir-378c, hsa-mir-486-2, oar-let-7a, oar-let-7f, oar-mir-103, oar-mir-10b, oar-mir-143, oar-mir-148a, oar-mir-152, oar-mir-16b, oar-mir-181a-2, oar-mir-200a, oar-mir-200b, oar-mir-200c, oar-mir-23a, oar-mir-26a, oar-mir-29b-1, oar-mir-30a, oar-mir-99a, bta-mir-664b, chi-let-7a, chi-let-7f, chi-mir-103, chi-mir-10b, chi-mir-125b, chi-mir-126, chi-mir-141, chi-mir-143, chi-mir-146a, chi-mir-148a, chi-mir-148b, chi-mir-155, chi-mir-15a, chi-mir-15b, chi-mir-16a, chi-mir-16b, chi-mir-184, chi-mir-192, chi-mir-200a, chi-mir-200b, chi-mir-200c, chi-mir-215, chi-mir-21, chi-mir-223, chi-mir-224, chi-mir-2318, chi-mir-23a, chi-mir-24, chi-mir-26a, chi-mir-27b, chi-mir-296, chi-mir-29a, chi-mir-29b, chi-mir-29c, chi-mir-30a, chi-mir-30e, chi-mir-342, chi-mir-378, chi-mir-451, chi-mir-499, chi-mir-671, chi-mir-99a, chi-mir-99b, bta-mir-378d, ssc-mir-378b, oar-mir-29b-2, ssc-mir-141, ssc-mir-200b, ssc-mir-223, bta-mir-148d
Ye et al. (2012) examined miRNA expression in the duodenum of E. coli F18-sensitive and -resistant weaned piglets and identified 12 candidate miRNA (ssc-miR-143, ssc-let-7f, ssc-miR-30e, ssc-miR-148a, ssc-miR-148b, ssc-miR-181a, ssc-miR-192, ssc-miR-27b, ssc-miR-15b, ssc-miR-21, ssc-miR-215, and ssc-miR-152) disease markers. [score:5]
Additionally, a number of miRNAs including miR-148a, miR-26a, miR-21-5p, miR-27b, miR-143, bta-miR-30a-5p, let-7a-5p, let-7f, miR-10b, and miR-99a-5p are highly expressed in bovine mammary gland/mammary epithelial cells (Li et al., 2012a, 2014a; Jin et al., 2014a; Le Guillou et al., 2014) suggesting roles in the lactation process and mammary gland functions. [score:3]
Similarly, Jin et al. (2014a) demonstrated a differential expression of nine miRNAs (bta-miR-184, miR-24-3p, miR-148, miR-486, and let-7a-5p, miR-2339, miR-499, miR-23a, and miR-99b) upon challenge of MACT-cells (bovine mammary epithelia cell line) with heat inactivated E. coli and S. aureus bacteria. [score:3]
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[+] score: 9
Of these, 40 miRNAs are minimally expressed (0 < average signals ≤ 100; p ≤ 0.01), 77 miRNAs are modestly expressed 100 < average signals ≤ 1,000; p ≤ 0.01), 85 miRNAs were highly expressed (1,000 < average signals ≤ 10,000), and, in particular, 20 miRNAs were extremely highly expressed in the anterior pituitary (average signals ≥ 10,000; p ≤ 0.01), including ssc-miR-7, Y-90, ssc-miR-26a, ssc-miR-125b, Y-1, ssc-miR-125a, Y-77, ssc-let-7g, ssc-miR-29a, ssc-let-7i, ssc-let-7a, ssc-let-7f, ssc-miR-148a, ssc-miR-21, ssc-miR-335, ssc-miR-30b-5p, ssc-miR-191, ssc-miR-29c, ssc-miR-23b, and ssc-miR-23a (Fig 1C). [score:9]
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[+] score: 9
In total, the QPCR results have consisted with RNA-Seq results, three TDETGs (COL6A2, ITGB1 and CD34) and four DE miRNAs (miR-10b, miR-148a-3p, miR-181d-5p, miR-181a) were down-regulated; four TDETGs (SPATA24, KHDBPS3, TSGA10, GGNBP) and one DE miRNA (miR-133a-3p) were up-regulated by two methods. [score:7]
Nine TDETGs (CCNI, SPATA24, NEURL, KHDBPS3, TSGA10, GGNBP2, COL6A2, ITGB1 and CD34) and eight DE miRNAs (miR-301, miR-194b-5p, miR-10b, miR-148a-3p, miR-181d-5p, miR-181a, miR-133a-3p and miR-145-5p) from Table 3 were confirmed via real-time RT-PCR to be involved in spermatogenesis and testicular development. [score:2]
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[+] score: 9
In G2 (up-regulated), miR-148a was markedly the most different between breeds at 2 dpn. [score:4]
In a comparison between the pig breeds, 49 dpc showed the most significant differences in G1 (up-regulated), in which miR-378, miR-30a, miR-148a, and miR-127 showed drastic changes. [score:4]
Figure  3 shows that in G1 (up), we clearly found that 49 dpc showed the most significant differences between breeds; in this group, miR-378, miR-148a and miR-127 showed drastic changes. [score:1]
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[+] score: 8
The miR-148a-3p was down-regulated at 3 dpi in our data, but the qRT-PCR results showed that it was up-regulated at all the time point we detected (Fig. 2c, d). [score:7]
2. We randomly chose three DEmiRNAs (miR-204, miR-148a-3p, miR-424-5p) in the lung tissues of Tongcheng pigs from our data. [score:1]
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[+] score: 8
For example, miR-29, miR-181 and miR-148a can promote myoblast differentiation by inhibiting the expression of downstream target genes Akt3, Hox-A1 and ROCK1 at protein levels [10– 12]. [score:7]
MiR-148a, miR-206 and miR-214 have been shown to be similar to miR-322/424 and miR-503 [12, 33, 34]. [score:1]
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[+] score: 7
In addition, 10 other miRNAs (miR-378-1/-2-3p, miR-127-3p, miR-191-5p, miR-486-2-5p, miR-143-3p, miR-10a-5p, miR-148a-3p, miR-99a-5p, miR-30e-5p, and miR-199a-1/-2-5p) (Fig. 2) in the set of the top 10 most highly expressed unique miRNAs over the five muscle development stages are related to various cell proliferation, myogenesis, and apoptosis responses. [score:4]
Another miRNA, miR-148a, belong to the top ten expressed miRNAs only in E90 period, which was in line with previous finding that the average abundance of this miRNA before birth was eight times higher than that in postnatal (Qin et al., 2013). [score:3]
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[+] score: 7
For example, DNMT1, PTEN and WNT1 were the putative target genes of miR-148/152 family. [score:3]
Furthermore, DNMT1 was found in breast cancer and verified as a target for miR-148 [30]. [score:3]
Chen Y. Song Y. X. Wang Z. N. The microRNA-148/152 family: Multi-faceted players Mol. [score:1]
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[+] score: 4
Further, miR-18a, miR-24, miR-146a, miR-148a and miR-214 were predicted to target the apoptosis pathway (Table 3). [score:3]
While 23 miRNAs were DE in PS samples (Table 1), only three were DE in LS (miR-21, miR-340, miR-148a). [score:1]
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[+] score: 3
Our results were consistent with previous studies, which demonstrated miR-29 targeting to the collagen family members such as COL4A1, COL1A2 and COL1A1 at ADU time points, miR-148 to the MITF and EIF4BP2 genes at early embryonic stage, and miR-487 to the IRS1 gene at the middle stage [23, 24]. [score:3]
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[+] score: 3
Among miRNAs preferentially expressed in the heart (Figure 4) mir-148a, mir-101, and mir-138 are particularly important. [score:3]
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24
[+] score: 3
The top 10 highest expressed miRNAs detected by deep sequencing were miR-148a, miR-101, miR-143-3p, miR-122, miR-30a-5p, miR-21, miR-30c, miR-192, miR-27b and miR-24 (Table S1). [score:3]
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[+] score: 2
The genetic regulated miRNAs miR-34a, miR-30e, miR-148-3p, miR-204, miR-181-5p, miR-143-5p and let-7g were also correlated with haematological and biochemical traits. [score:2]
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[+] score: 2
Other miRNAs from this paper: ssc-mir-122, ssc-mir-135-1, ssc-mir-135-2, ssc-mir-19a, ssc-mir-20a, ssc-mir-224, ssc-mir-24-1, ssc-mir-323, ssc-mir-140, ssc-mir-183, ssc-mir-214, ssc-mir-27a, ssc-mir-325, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-107, ssc-mir-136, ssc-mir-153, ssc-mir-18a, ssc-mir-186, ssc-mir-196a-2, ssc-mir-204, ssc-mir-21, bta-mir-18b, bta-let-7f-2, bta-mir-101-2, bta-mir-103-1, bta-mir-148a, bta-mir-18a, bta-mir-20a, bta-mir-21, bta-mir-221, bta-mir-27a, bta-mir-27b, bta-mir-107, bta-mir-140, bta-mir-20b, bta-mir-215, bta-let-7d, bta-mir-17, bta-mir-186, bta-mir-199b, bta-mir-210, bta-mir-214, bta-mir-450a-2, bta-let-7g, bta-mir-24-2, bta-let-7a-1, bta-let-7f-1, bta-mir-122, bta-let-7i, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-15a, bta-mir-19a, bta-mir-204, ssc-mir-15a, ssc-mir-17, ssc-mir-199b, ssc-mir-210, ssc-mir-221, bta-mir-101-1, bta-mir-133a-2, bta-mir-133a-1, bta-mir-135a-2, bta-mir-135a-1, bta-mir-135b, bta-mir-136, bta-mir-146b, bta-mir-153-1, bta-mir-153-2, bta-mir-183, bta-mir-24-1, bta-mir-196a-2, bta-mir-196a-1, bta-mir-196b, bta-mir-224, bta-mir-323, ssc-mir-101-1, ssc-mir-101-2, ssc-mir-133a-1, ssc-mir-450a, ssc-mir-146b, ssc-mir-215, bta-mir-1343, bta-mir-2320, bta-mir-2326, bta-mir-2366, bta-mir-2411, bta-mir-2483, bta-mir-450a-1, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-103-2, ssc-mir-27b, ssc-mir-24-2, ssc-mir-196b-1, ssc-mir-450b, ssc-mir-450c, ssc-mir-133a-2, ssc-let-7a-2, ssc-mir-18b, ssc-mir-1343, ssc-mir-2320, bta-mir-450b, ssc-let-7d, ssc-let-7f-2, ssc-mir-20b-1, ssc-mir-20b-2, ssc-mir-196a-1, ssc-mir-196b-2, ssc-mir-2366-1, ssc-mir-2366-2, ssc-mir-2411, ssc-mir-2483
Obviously, high-abundant miRNAs (let-7c, let-7f, miR-148a, miR-21 and miR-24) had higher edited probability in backfat tissue. [score:1]
However, the total reads of two conserved miRNAs (ssc-miR-148a and ssc-let-7c) were striking, almost 1.7 million and 1.6 million, respectively (Table S2). [score:1]
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[+] score: 1
Previous studies have demonstrated that the myomiRs miR-1, miR-133a/b, miR-206, miR-486, miR-26a, miR-27b, miR-378, miR-148a and miR-181 are highly enriched in skeletal muscle and play a key role in skeletal muscle metabolism [28, 29, 30, 31]. [score:1]
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