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5 publications mentioning rno-mir-539

Open access articles that are associated with the species Rattus norvegicus and mention the gene name mir-539. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

[+] score: 48
Based on microarray analysis and further validation by qRT-PCR, we postulated that the upregulation of miR-376b-5p and miR-539 is involved in myocardial ischemia and M [3]-mAChR may protect against myocardial ischemia via the downregulation of miR-376b-5p and miR-539 expression. [score:9]
To confirm the expression changes of miR-376b-5p and miR-539 in myocardial ischemia, next we performed qRT-PCR analysis and found that miR-376b-5p and miR-539 were significantly upregulated upon myocardial ischemia and down-regulated by choline (P<0.05, Figure 1C and D). [score:9]
These data demonstrate that miR-376b-5p and miR-539 are significantly upregulated in ischemic heart of the rats and M [3]-mAChR agonist choline could reverse their upregulation. [score:7]
While miR-199a, miR-376b-5p, miR-539 and miR-106 were all significantly upregulated (more than 3 times compared to the control), only miR-376b-5p and miR-539 were downregulated to the level similar to the control group after treatment with choline. [score:6]
H9c2 cells were pre -treated with H [2]O [2] (50 µM) for 12 h and then transfected with miR-376b-5p negative control (miR-NC), miR-376b-5p, miR-539, and 2′-O-methyl -modified antisense oligoribonucleotides of miR-376b-5p (AMO-376b-5p, miR-376b-5p inhibitor) for 24 h. (A). [score:3]
Rat miR-376b-5p, miR-539, 2′-O-methyl -modified antisense oligoribonucleotides of miR-376b-5p (AMO-376b-5p, miR-376b-5p inhibitor) and miR-376b-5p negative control (miR-NC) were synthesized by Shanghai GenePharma. [score:3]
However, miR-539 had no significant effect on H [2]O [2] -induced inhibition of H9c2 cells viability (Figure 2A). [score:3]
qRT-PCR for miR-376b-5p, miR-539 was performed on a GeneAmp 5700 thermocycler using mirVanaTM miRNA Detection Kit following the manufacturer's instructions. [score:1]
To determine the involvement of miR-376b-5p and miR-539 in myocardial ischemia, we used H9c2 cells as an in vitro mo del. [score:1]
MiR-376b-5p, not miR-539, reduced cell viability in H [2]O [2] -treated H9c2 cells. [score:1]
H9c2 cells were transfected with miR-NC (100 nM), miR-376b-5p (100 nM), miR-539 (100 nM), miR-376b-5p (100 nM) and AMO-376b-5p (100 nM) using lipofectamine 2000 according to the manufacturer's instruction and incubated at 37°C for 24 h. Subsequently, cells were maintained in the culture until analysis [11]. [score:1]
MiR-376b-5p promotes H [2]O [2] induced H9c2 cells injuryTo determine the involvement of miR-376b-5p and miR-539 in myocardial ischemia, we used H9c2 cells as an in vitro mo del. [score:1]
Therefore, miR-376b-5p and miR-539 appear to be involved in M [3]-mAChR mediated cardioprotection against myocardial ischemia. [score:1]
Notably, miR-376b-5p but not miR-539 was implicated in reduced H9c2 cells viability induced by H [2]O [2]. [score:1]
These results suggest that miR-539 is unlikely involved in myocardial ischemia and we focused on miR-376b-5p in the following experiments. [score:1]
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[+] score: 18
miR-539, the highest upregulated miRNA have 271 conserved gene target however functional target has not been reported in the literature. [score:8]
miR-539 is upregulated to high level (214 fold) in IR samples and were further up-regulated in resveratrol preteated samples. [score:7]
The targets of miR-539 obtained by computational analyses include matrix metallopeptidase 20, fibroblast growth factor 14, clathrin, light polypeptide, osteoprotegerin and transcription factors like forkhead box B1, which may have roles in cardiac remo deling. [score:3]
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[+] score: 11
Wang K CARL lncRNA inhibits anoxia -induced mitochondrial fission and apoptosis in cardiomyocytes by impairing miR-539 -dependent PHB2 downregulationNat. [score:6]
Researchers also demonstrated that miR-539 binds PHB2 and suppresses its expression to induce mitochondrial fission and apoptosis [120]. [score:5]
[1 to 20 of 2 sentences]
[+] score: 8
Likewise, the arterial highly expressed microRNA miR-539 showed elevated expression in the abnormal tissue in autistic disorder and medulloblastoma [18, 22]. [score:5]
But the capability of circulating miR-539 as the disease biomarker has not been tested. [score:3]
[1 to 20 of 2 sentences]
[+] score: 3
Other miRNAs from this paper: rno-mir-103-2, rno-mir-103-1, rno-mir-107, rno-mir-122, rno-mir-370
Gracia A. Miranda J. Fernández-Quintela A. Eseberri I. Garcia-Lacarte M. Milagro F. I. Martínez J. A. Aguirre L. Portillo M. P. Involvement of miR-539–5p in the inhibition of de novo lipogenesis induced by resveratrol in white adipose tissueFood Funct. [score:3]
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