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8 publications mentioning rno-mir-485

Open access articles that are associated with the species Rattus norvegicus and mention the gene name mir-485. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 81
Other miRNAs from this paper: rno-mir-326, rno-mir-330, rno-mir-761, rno-mir-666
Neurite outgrowth was inhibited by overexpression of a miR-485-5p mimic (p < 0.001) and enhanced by a miR-485 inhibitor (p < 0.001). [score:7]
Consistent with predicted function of miR-485-5p based on target analysis and GO enrichment for regulation of neuritogenesis and cytoskeletal organization (figure 4 b), miR-485-5p overexpression dramatically reduced NGF -dependent neurite outgrowth (figure 5 a, b, p < 0.001, n = 36 fields from six transfections). [score:6]
miR-485 overexpression inhibits NGF -induced neurite outgrowth in PC12 cells. [score:5]
Figure 7. miR-485 overexpression controls tau expression and axonal outgrowth in hippocampal neurons. [score:5]
Target prediction and GO analysis of miR-326-3p/330-5p, miR-485-5p (miR-485-5p/1698/1703/1962), miR-666-3p and miR-761-5p showed enrichment for terms associated with synaptic transmission, plasticity, neurotransmitter synthesis and vesicular release, in both miRNA targets (figure 4 a) and GO terms (figure 4 b and table 3; and the electronic supplementary material, table S2). [score:5]
Shown are representative examples of untreated (control) and neurons transfected with either a miRNA mimic or inhibitor showing that axonal outgrowth is regulated by miR-485-5p. [score:4]
miR-485-5p overexpression reduced the extent of axonal outgrowth in hippocampal neurons. [score:3]
Moreover, miR-485 and miR-666 are intronic miRNAs that are part of the large Dlk1-Dio3 imprinted locus [52, 53] containing at least 53 miRNAs expressed in the CNS [54]. [score:3]
Overexpression of miR-485-5p modestly decreased tau transcript abundance (a) and protein levels (b) in hippocampal neurons. [score:3]
Several of the corresponding miRNAs, including miR-326/miR-330, miR-485, miR-666 and miR-761 were found to be developmentally regulated in the hippocampus. [score:3]
Expression levels for miR-330-5p only modestly increased by 21 days post parturition (miR-456 expression, corresponding to motif 3 and sharing a seed motif with miR-485-5p was very low at all time points measured). [score:3]
Shown in (a) are representative examples of PC12 cells treated with 50 ng ml [−1] NGF and co -transfected with DsRed and miR-485 mimic, miR -inhibitor and negative controls, and analysed at 96 h. Scale bar, 25 μm. [score:3]
Hippocampal cultures at 7 DIV were co -transfected with 2 μg DsRed-C1 (Clontech, Mountainview, CA, USA) and either 25 pmol of miR-485-5p mimic, miR-485-5p inhibitor, miR negative controls, or distilled H [2]O (Ambion, Austin, TX, USA) and Lipofectamine 2000 (Life Technologies), as previously described [24]. [score:3]
Figure 6. miR-485 decreases tau expression in hippocampal neurons. [score:3]
Ingenuity pathway analysis was performed on predicted targets of miR-326-3p/miR-330-5p, miR-485-5p, miR-666-3p and miR-761 using the T argetS can v. 6.2 algorithm [49– 51]. [score:3]
One of the miRNAs identified here, miR-485-5p, was shown to regulate NGF -induced neurite outgrowth in PC12 cells (figure 5) and axonal development in hippocampal neurons (figures 6 and 7). [score:3]
Conversely, transfection with a miR-485 inhibitor significantly increased neurite outgrowth (p < 0.01). [score:3]
miR-485 regulates neurite outgrowth in PC12 cells. [score:2]
The effects on miR-485-5p on both neurite outgrowth in PC12 cells and axonal markers further support a function of this miRNA on axonal development. [score:2]
Transcript levels for miR-326-3p, miR-485-5p, miR-666-3p and miR-761 increased by threefold to 12-fold by 14 days post parturition (figure 4 c) (n = 3 animals per time point). [score:1]
We found that four of the discovered motifs (figure 3 a) in the destabilized gene set corresponded to seed domains of miRNAs miR-326-3p/miR-330-5p, miR-485-5p, miR-666-3p and miR-761. [score:1]
Of the miRNA motifs that were identified, motifs corresponding to seed domains for miR-666-3p and miR-485-5p were over-represented in the variable-width motif set (electronic supplementary material, figure S2). [score:1]
Effects of miR-485 on NOI were highly significant by one-way ANOVA (F [4,25] = 26.15, p < 0.0001). [score:1]
We tested the function of miR-485-5p on NGF -dependent neurite outgrowth in PC12 cells, a well-established mo del of neurite outgrowth. [score:1]
The function of miR-485-5p on axons was next tested in hippocampal neurons. [score:1]
Figure 5. miR-485 blocks NGF -induced neurite outgrowth in PC12 cells. [score:1]
Furthermore, one of these miRNAs, miR-485, predicted to affect central nervous system (CNS) morphogenesis and neurite outgrowth, controls nerve growth factor (NGF) -induced neurite outgrowth in PC12 cells. [score:1]
Furthermore, analysis on a second microarray platform also independently showed enrichment for miR-485-5p (figure 3, motif 1b). [score:1]
Furthermore, miR-485-5p overexpression reduced the extent of axonal outgrowth in hippocampal neurons as measured by immunoreactivity to tau protein (figure 7 a) and the pan-axonal neurofilament marker SMI312 (figure 7 b). [score:1]
We previously reported on the function of miR-485-5p on homeostatic changes in synapses through action on the presynaptic vesicle protein SV2A [24]. [score:1]
miR-485 top canonical pathways. [score:1]
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2
[+] score: 23
Among all the differentially expressed miRNAs, miR-485 had the most regulatory targets (32 target genes) and among the differentially expressed mRNAs, phosphoprotein enriched in astrocytes 15 (Pea15a), guanylate cyclase 1, soluble, alpha 3 (Gucy1a3), PDZK1 interacting protein 1 (Pdzk1ip1), connective tissue growth factor (Ctgf), inositol 1,4,5-triphosphate 3-kinase C (Itpkc) and heat shock protein 5 (Hspa5) had most regulatory miRNAs (each had 4 potential controlling miRNAs). [score:11]
Among the miRNAs, miR-214, miR-199a-5p, miR-150, miR-199a-3p, miR-351, miR-145, miR-764, miR-497 and miR-92b were upregulated, whilst miR-7a, miR-325-5p, miR-485, miR-708, miR-344-3p, let-7f, miR-26b, miR-129, miR-29c and let-7a were downregulated. [score:7]
Several miRNAs, including miR-485, were connected with a number of putative target genes and certain genes, including Pea15a, Gucy1a3, Pdzk1ip1, Ctgf, Itpkc and Hspa5 were found to be regulated by several miRNAs. [score:4]
These miRNAs include miR-214, miR-199a-5p, miR-150, miR-351, miR-145, miR-92b, miR-7a, miR-485, miR-708, let-7f, miR-26b, miR-129, miR-29c and let-7a. [score:1]
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3
[+] score: 18
Furthermore, expression levels of miR-485-3p, which has 2 consensus binding sites in the FPN 3′UTR (at bases 448-454 and 618–625, respectively) and has been shown to be involved in the inhibition of FPN expression by iron deficiency in HepG2 cells [30], were not altered in quercetin -treated Caco-2 cells (Figure 6D). [score:7]
While there was no significant effect of quercetin on miR-485-3p expression in Caco-2 cells, we identified miR-17-3p as a quercetin -induced candidate regulator of FPN mRNA expression. [score:6]
Interestingly, miRNAs have been shown to play a role in modulating the expression of a number of key players in iron homeostatic regulation, including hepcidin (miR-122; [47]), DMT1 (Let-7d; [48]) and FPN (miR-485-3p; [30]). [score:4]
Figure S2 Predicted binding sites for miR17-3p, miR17-5p and miR485-3p in human FPN 3′UTR. [score:1]
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4
[+] score: 5
The 3′UTR of human, mouse and rat p53 mRNA all have miR-485 conserved predicted target sites, but only the 3′UTRs of human p53 mRNA has miR-125b target site, (B–D) Quantitative RT-PCR assays were performed to examine p53 mRNA levels in three cell types (H460, NIH/3T3 and H9C2) treated with p53 siRNA and other miRNAs for 48 h. GAPDH served as the internal control (top). [score:4]
In fact, not only miR-138, miR-125b regulation of p53 also has species specificity, compared with miR-485 without this feature. [score:1]
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5
[+] score: 3
org) revealed several miRNA that might interact with POMC mRNA untranslated region, including miR-488, miR-485, miR-384-3p, miR-383, miR-377, miR-485-5p and miR-181 (family). [score:3]
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6
[+] score: 1
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-21, hsa-mir-26b, hsa-mir-27a, hsa-mir-29a, hsa-mir-30a, hsa-mir-33a, hsa-mir-98, hsa-mir-29b-1, hsa-mir-29b-2, mmu-let-7g, mmu-let-7i, mmu-mir-27b, mmu-mir-29b-1, mmu-mir-30a, mmu-mir-30b, mmu-mir-126a, mmu-mir-133a-1, mmu-mir-135a-1, mmu-mir-141, mmu-mir-194-1, mmu-mir-200b, hsa-mir-30c-2, hsa-mir-30d, mmu-mir-30e, hsa-mir-203a, hsa-mir-211, hsa-mir-218-1, hsa-mir-218-2, hsa-mir-200b, mmu-mir-300, mmu-let-7d, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-30b, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-135a-1, hsa-mir-135a-2, hsa-mir-141, hsa-mir-194-1, mmu-mir-30c-1, mmu-mir-30c-2, mmu-mir-30d, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-21a, mmu-mir-26b, mmu-mir-29a, mmu-mir-29c, mmu-mir-27a, mmu-mir-98, mmu-mir-326, rno-mir-326, rno-let-7d, rno-mir-343, rno-mir-135b, mmu-mir-135b, hsa-mir-200c, mmu-mir-200c, mmu-mir-218-1, mmu-mir-218-2, mmu-mir-33, mmu-mir-211, mmu-mir-29b-2, mmu-mir-135a-2, hsa-mir-194-2, mmu-mir-194-2, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-30e, hsa-mir-326, hsa-mir-135b, mmu-mir-133a-2, mmu-mir-133b, hsa-mir-133b, rno-let-7a-1, rno-let-7a-2, rno-let-7b, rno-let-7c-1, rno-let-7c-2, rno-let-7e, rno-let-7f-1, rno-let-7f-2, rno-let-7i, rno-mir-21, rno-mir-26b, rno-mir-27b, rno-mir-27a, rno-mir-29b-2, rno-mir-29a, rno-mir-29b-1, rno-mir-29c-1, rno-mir-30c-1, rno-mir-30e, rno-mir-30b, rno-mir-30d, rno-mir-30a, rno-mir-30c-2, rno-mir-33, rno-mir-98, rno-mir-126a, rno-mir-133a, rno-mir-135a, rno-mir-141, rno-mir-194-1, rno-mir-194-2, rno-mir-200c, rno-mir-200a, rno-mir-200b, rno-mir-203a, rno-mir-211, rno-mir-218a-2, rno-mir-218a-1, rno-mir-300, hsa-mir-429, mmu-mir-429, rno-mir-429, hsa-mir-485, hsa-mir-511, hsa-mir-532, mmu-mir-532, rno-mir-133b, mmu-mir-485, hsa-mir-33b, mmu-mir-702, mmu-mir-343, mmu-mir-466b-1, mmu-mir-466b-2, mmu-mir-466b-3, hsa-mir-300, mmu-mir-511, rno-mir-466b-1, rno-mir-466b-2, rno-mir-532, rno-mir-511, mmu-mir-466b-4, mmu-mir-466b-5, mmu-mir-466b-6, mmu-mir-466b-7, mmu-mir-466b-8, hsa-mir-3120, rno-mir-203b, rno-mir-3557, rno-mir-218b, rno-mir-3569, rno-mir-133c, rno-mir-702, rno-mir-3120, hsa-mir-203b, mmu-mir-344i, rno-mir-344i, rno-mir-6316, mmu-mir-133c, mmu-mir-21b, mmu-let-7j, mmu-mir-21c, mmu-mir-30f, mmu-let-7k, mmu-mir-3569, rno-let-7g, rno-mir-29c-2, rno-mir-29b-3, rno-mir-466b-3, rno-mir-466b-4, mmu-mir-203b
Type of site Context+ Context Structure Energy Is experimental validated rno-miR-326-5p MIMAT0017028 3 8mer 7mer-m8 imperfect −0.442 −0.242 431 −65.97 TRUE rno-miR-485-5p MIMAT0003203 2 7mer-m8 −0.343 −0.372 290 −34.96 TRUE rno-miR-300-5p MIMAT0004743 1 8mer −0.338 −0.421 156 −15.16 TRUE rno-miR-702-5p MIMAT0017884 1 8mer −0.317 −0.274 142 −13.86 TRUE rno-miR-203b-3p MIMAT0017800 2 7mer-m8 −0.298 −0.421 295 −29.93 TRUE rno-miR-33-3p MIMAT0017104 2 8mer 7mer-m8 −0.297 −0.813 305 −22.7 TRUE rno-miR-466b-3p MIMAT0017285 1 8mer −0.295 −0.47 159 −15.26 TRUE rno-miR-532-5p MIMAT0005322 1 7mer-m8 −0.268 −0.302 151 −10.71 TRUE rno-miR-511-5p MIMAT0012829 1 7mer-m8 −0.268 −0.302 152 −10.37 TRUE rno-miR-343 MIMAT0000591 1 7mer-m8 −0.262 −0.24 140 −13.75 TRUE rno-miR-203a-3p MIMAT0000876 1 8mer −0.245 −0. [score:1]
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7
[+] score: 1
It has been reported that miR-485 controls synapse formation and dendritic spine number in an activity-related manner to maintain homeostatic plasticity [30]. [score:1]
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8
[+] score: 1
Other miRNAs from this paper: mmu-mir-1a-1, mmu-mir-127, mmu-mir-134, mmu-mir-136, mmu-mir-154, mmu-mir-181a-2, mmu-mir-143, mmu-mir-196a-1, mmu-mir-196a-2, mmu-mir-21a, rno-mir-329, mmu-mir-329, mmu-mir-1a-2, mmu-mir-181a-1, mmu-mir-181b-1, mmu-mir-181c, mmu-mir-375, mmu-mir-379, mmu-mir-181b-2, rno-mir-21, rno-mir-127, rno-mir-134, rno-mir-136, rno-mir-143, rno-mir-154, rno-mir-181c, rno-mir-181a-2, rno-mir-181b-1, rno-mir-181b-2, rno-mir-196a, rno-mir-181a-1, mmu-mir-196b, rno-mir-196b-1, mmu-mir-412, mmu-mir-370, oar-mir-431, oar-mir-127, oar-mir-432, oar-mir-136, mmu-mir-431, mmu-mir-433, rno-mir-431, rno-mir-433, ssc-mir-181b-2, ssc-mir-181c, ssc-mir-136, ssc-mir-196a-2, ssc-mir-21, rno-mir-370, rno-mir-412, rno-mir-1, mmu-mir-485, mmu-mir-541, rno-mir-541, rno-mir-493, rno-mir-379, mmu-mir-668, bta-mir-21, bta-mir-181a-2, bta-mir-127, bta-mir-181b-2, bta-mir-181c, mmu-mir-181d, mmu-mir-493, rno-mir-181d, rno-mir-196c, rno-mir-375, mmu-mir-1b, bta-mir-1-2, bta-mir-1-1, bta-mir-134, bta-mir-136, bta-mir-143, bta-mir-154a, bta-mir-181d, bta-mir-196a-2, bta-mir-196a-1, bta-mir-196b, bta-mir-329a, bta-mir-329b, bta-mir-370, bta-mir-375, bta-mir-379, bta-mir-412, bta-mir-431, bta-mir-432, bta-mir-433, bta-mir-485, bta-mir-493, bta-mir-541, bta-mir-181a-1, bta-mir-181b-1, ssc-mir-1, ssc-mir-181a-1, mmu-mir-432, rno-mir-668, ssc-mir-143, ssc-mir-181a-2, ssc-mir-181b-1, ssc-mir-181d, ssc-mir-196b-1, ssc-mir-127, ssc-mir-432, oar-mir-21, oar-mir-181a-1, oar-mir-493, oar-mir-433, oar-mir-370, oar-mir-379, oar-mir-329b, oar-mir-329a, oar-mir-134, oar-mir-668, oar-mir-485, oar-mir-154a, oar-mir-154b, oar-mir-541, oar-mir-412, mmu-mir-21b, mmu-mir-21c, ssc-mir-196a-1, ssc-mir-196b-2, ssc-mir-370, ssc-mir-493, bta-mir-154c, bta-mir-154b, oar-mir-143, oar-mir-181a-2, chi-mir-1, chi-mir-127, chi-mir-134, chi-mir-136, chi-mir-143, chi-mir-154a, chi-mir-154b, chi-mir-181b, chi-mir-181c, chi-mir-181d, chi-mir-196a, chi-mir-196b, chi-mir-21, chi-mir-329a, chi-mir-329b, chi-mir-379, chi-mir-412, chi-mir-432, chi-mir-433, chi-mir-485, chi-mir-493, rno-mir-196b-2, bta-mir-668, ssc-mir-375
Other families that had a high abundance of reads were miR-134, miR-136, miR-154, miR-370, miR-412, miR-431, miR-432, miR-433, miR-485, miR-493, miR-541; a total of 11 miRNA families. [score:1]
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