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4 publications mentioning gga-mir-202

Open access articles that are associated with the species Gallus gallus and mention the gene name mir-202. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 74
Among them miR-202-3p was predicted to target 34 genes, miR-202-5p targeted 18 genes, miR-147 targeted 17 genes, and miR-126-3p targeted 9 genes. [score:9]
Our analysis of the differently expressed miRNAs revealed four miRNA (miR-202-5p/3p, miR-126-3p, miR-147) that were differentially expressed in all three comparisons (SSC vs. [score:5]
According to manual of Lipofectamine 2000 (Invitrogen, Shanghai, China), we transfected the miR-202-5p mimics and inhibitor in PGCs and DF-1. After 48 hours, collected the cells and detected relative expression of miR-202-5p and related genes. [score:5]
The expression patterns of the 4 common differentially expressed miRNAs (miR-202-3p, miR-202-5p, miR-147 and miR-126-3p) were shown in Fig 6e. [score:5]
From this analysis, miR-202-3p/5p showed the highest expression in Sp and miR-147 and miR-100-5p, miR-21-5p present the high expression in SSCs. [score:5]
PLoS One 7. 22 Wainwright EN, Jorgensen JS, Kim Y, Truong V, Bagheri-Fam S, Davidson T, et al (2013) SOX9 regulates microRNA miR-202-5p/3p expression during mouse testis differentiation. [score:4]
During early testis development, miR-202-5p/3p have been reported to play a conserved expression role in gonad, and SOX9 was identified as the downstream gene that was the testis-determining factor [22]. [score:4]
In conclusion, we identified several novel miRNAs such as miR-202-5p, and their target genes LIMK2, which may be involved in spermatogenesis and germline stem cell development in chicken. [score:4]
There were 18 genes targeted by miR-202-5p. [score:3]
MiR-202-5p/3p was up-regulated in SSCs vs. [score:3]
Venn analysis of these miRNA identified 4 miRNAs expressed in all samples analyzed: miR-202-3p, miR-202-5p, miR-147 and miR-126-3p (Fig 6b). [score:3]
In human, miR-202-5p was reported to be up-regulated by 17-fold (P <0.00001) in normal fertile men compared with men with azoospermia [23], which implied that miR-202-5p played an important role in spermatogenesis. [score:3]
In order to verify the relationship between miR-202-5p and LIMK2, we transfected the miR-202-5p mimics and inhibitor in DF-1 and PGCs, respectively. [score:3]
In miR-202-5p, PGCs -mimics group was 538.3 times of other two groups but in LIMK2 the expression was the lowest in three groups (Fig 8c and 8d). [score:3]
Through RT-qPCR and dual-luciferase assay, we confirmed miR-202-5p bind to the LIMK2 in chicken and LIMK2 showed the highest expression in PGCs. [score:2]
Thus, miR-202-5p have an effect on LIMK2 and involved in germ cell development. [score:2]
miR-202-5p bind to LIMK2 genes in chicken. [score:1]
Compared with control group, the experiment group showed lower fluorescence activity than control group which indicated that miR-202-5p could restrain LIMK2 transcript regulation (Fig 8e) We performed the immunofluorescence on PGCs (mouse anti-chicken C-kit antibody) and SSCs (rabbit polyclonal antibody to integrin alpha 6) (Fig 1). [score:1]
MiR-202-5p bind to LIMK2 genes in chickenFrom above the result, we chose miR-202-5p as candidate factor to verify relationship between miR-202-5p and LIMK2. [score:1]
Compared with control group, the experiment group showed lower fluorescence activity than control group which indicated that miR-202-5p could restrain LIMK2 transcript regulation (Fig 8e) Lowercase letters indicated p <= 0.01. [score:1]
The left one was miR-202-5p which present in SSCs either. [score:1]
This result indicated that the sequencing result was believable and there was a relationship between miR-202-5p and LIMK2. [score:1]
From above the result, we chose miR-202-5p as candidate factor to verify relationship between miR-202-5p and LIMK2. [score:1]
Thus we analysis miR-202-5p. [score:1]
Co -transfected the pGL3-LIMK2, pRL-TK and miR-202-5p mimics via Lipofectamine 2000 in DF-1. Ratio of 50:1 for pGL3-LIMK2: pRL-TK and the 60nM for miR-202-5p mimics, pGL3-Basic as control. [score:1]
First we used DF-1 to investigate the efficiency of miR-202-5p mimic and inhibitor. [score:1]
We construct the luciferase vectors to verify the relationship between miR-202-5p and LIMK2. [score:1]
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2
[+] score: 19
Reduced miR-202* expression is correlated with reduced expression of the testis -associated genes DMRT1 and SOX9, and up-regulation of the ovary -associated genes FOXL2 and CYP19A1 (aromatase) [55]. [score:8]
In our study, gga-miR-202* was down-regulated by 3.2-fold and gga-miR-31 was down-regulated by 1.68-fold in sexually mature ovaries compared with immature ovaries, suggesting they are also involved in the sexual maturity of ovary. [score:6]
Similar expression patterns were found for gga-miR-199* and gga-miR-202* (Additional file 1: Table S3). [score:3]
Studies indicated that gga-miR-31, gga-miR-101, gga-miR-202 and gga-miR-202* may be involved in regulating gonadal differentiation in embryonic chicken gonads [54- 56]. [score:2]
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3
[+] score: 7
Some differentially expressed miRNAs had multiple predicted viral targets, such as gga-miR-202 which is predicted to target all nine AIV genes. [score:7]
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4
[+] score: 3
For example, the three members of the let-7 family (let-7a, let-7f, let-7k) are broadly expressed across tissues [36] and tissue enrichment has been found for miR-499-5p and −3p in heart [37], miR-122-5p in liver [38], miR-202-5p in testis [39] and gga-miR-107-3p in brain tissues [40] (Table 2). [score:3]
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