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15 publications mentioning gga-mir-375

Open access articles that are associated with the species Gallus gallus and mention the gene name mir-375. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 204
As downstream genes of the Hippo pathway, cyclin E and DIAP1 in mammals are significantly upregulated in the liver following the significant downregulation of gga-miR-375 in the liver, and YAP1 is significantly upregulated. [score:10]
mRNA expression of YAP1, cyclin E, and DIAP1 in the liver, blood, bone marrow, and spleen of ALV-J infected chickensBecause gga-miR-375 inhibited cell proliferation and invasion and suppressed YAP1 protein production at the cellular level, we checked whether gga-miR-375 targeted Hippo signalling effector YAP1 in ALV-J infected chickens at intervals of 10 days up to 60 days. [score:9]
The growth of DF-1 cells was suppressed along with YAP1 expression and significantly reduced when gga-miR-375 was overexpressed, and YAP1 appeared highly expressed in infected chickens, suggesting that YAP1 may be an oncogenic gene involved in ALV-J infection. [score:9]
Because gga-miR-375 inhibited cell proliferation and invasion and suppressed YAP1 protein production at the cellular level, we checked whether gga-miR-375 targeted Hippo signalling effector YAP1 in ALV-J infected chickens at intervals of 10 days up to 60 days. [score:7]
Moreover, by directly targeting Hippo signalling effector YAP1, gga-miR-375 may directly or indirectly affect cyclin E and DIAP1 during the early stages of ALV-J infection, resulting in a range of effects on tumour development. [score:7]
miR-375 was proposed as a candidate tumour suppressor miRNA in gastric carcinoma targeting 14-3-3zeta, Janus kinase 2, and phosphoinositide -dependent kinase 1 [24], [25] and was recognized to inhibit neuritis differentiation by lowering HuD levels [26]. [score:7]
The data from this study showed that gga-miR-375 was significantly downregulated in liver tissue of chickens 10 weeks post ALV-J infection, which inhibited cell proliferation and promoted cell apoptosis under serum starvation. [score:6]
Our data shows that gga-miR-375 directly targets YAP1, induces cell apoptosis, and weakens the Hippo pathway, suggesting that ALV-J viruses might inhibit gga-miR-375 to influence cell proliferation, invasion, and apoptosis and subsequently affect normal cell fate and tumorigenesis. [score:6]
gga-miR-375 expression was frequently downregulated in ALV-J induced cancer. [score:6]
Later, it was reported that miR-375 is commonly downregulated in human tumour tissues, which significantly increased cancer cell development [24], [25]. [score:5]
To further confirm YAP1 as a direct target of gga-miR-375, YAP1 protein expression was assayed 48 and 72 hours after transfection with gga-miR-375, miR-NC, or NT in DF-1 or CHO cells. [score:5]
Based on TargetScan and miRBase search, YAP1 was predicted as a potential target gene of gga-miR-375 (Figure 4A). [score:5]
To explore the role that gga-miR-375 plays in ALV-J carcinogenesis, TargetScan, miRBase, and RNAhybrid algorithms were employed to search for putative cellular protein-coding gene targets of gga-miR-375. [score:5]
miR-375 promotes palmitate -induced lipoapoptosis in insulin-secreting NIT-1 cells through the inhibition of myotrophin (V1) protein expression [28]. [score:5]
Overexpression of gga-miR-375 inhibited DF-1 cell proliferation and invasion. [score:5]
The putative binding sites for gga-miR-375 was inserted downstream of the stop codon of firefly luciferase in pmiRGLO Dual-Luciferase miRNA Target Expression Vector (Ambion, Promega, Beijing, People's Republic of China) as described previously [41] (designated as YAP1'UTR-wt). [score:5]
Our findings, to some extent, were in agreement with a report on miRNA-375 in which it was shown to target the Hippo signalling effector YAP in human liver cancer and to inhibit tumour propagation [27]. [score:5]
This finding suggests that YAP1 is a direct target gene of gga-miR-375. [score:4]
Ins, the gga-miR-375 was significantly downregulated in liver tissue from the ALV-J infected chickens from 20 days post infection (Figure 1D), which may serve as a biomarker for diagnostic purposes. [score:4]
These data suggested that gga-miR-375 may directly inhibit YAP1 protein production through binding to the 3′-UTR of YAP1. [score:4]
The gga-miR-375 significantly suppressed the expression of YAP1 compared to miR-NC and NT (Figure 4C). [score:4]
profiling was performed in SPF chicken livers of controls and animals infected with ALV-J NX0101 strain, and the results showed that gga-miR-375 was significantly downregulated in SPF chicken livers of infected chickens at 10 weeks (P<0.01; Figure 1 C). [score:4]
Here, for the first time, we show that YAP1 is a direct target of gga-miR-375. [score:4]
YAP1 is a direct gga-miR-375 target. [score:4]
Our previous study has shown that gga-miR-375 to be frequently downregulated in the livers of chickens 10-weeks post ALV-J infection [35]. [score:4]
These results suggested that gga-miR-375 inhibits cell proliferation and invasion. [score:3]
Cell proliferation reagent WST-1 assays showed that all three groups (mock, miR-NC, and gga-miR-375) displayed fewer cells and overexpression of gga-miR-375 significantly inhibited the proliferation of DF-1 cells from 48 hours after transfection (Figure 2A) compared to the NC (miR-NC) or the mock group. [score:3]
We also found that gga-miR-375 targets YAP1. [score:3]
There is substantial literature on miR-375 documenting this microRNA as a tumour suppressor in humans. [score:3]
To explore the role of gga-miR-375 in ALV-J carcinogenesis, we examined the effect of gga-miR-375 overexpression on the proliferation of DF-1 cell lines. [score:3]
The DAPI staining data suggests that gga-miR-375 overexpression remarkably increased serum starvation induced apoptosis in DF-1 cells (P<0.001; Figure 3A, 3B) at 48 and 72 hours. [score:3]
The luciferase activity of cells transfected with a gga-miR-375 precursor was significantly decreased compared to the NC (P<0.01; Figure 4B), indicating the mutation within the putative gga-miR-375 -binding site clearly abrogated the repression of luciferase activity caused by gga-miR-375 overexpression. [score:3]
gga-miR-375 and reference 5S rRNA, or the target genes and the reference gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were amplified, cloned, and used as standard controls to generate standard curves following a previously described protocol [42]. [score:3]
Overexpression of gga-miR-375 sufficiently enhanced serum starvation induced apoptosis, implying gga-miR-375 may also activate the Hippo pathway to augment apoptosis by transactivating growth-promoting genes through the TEAD binding domain of YAP1. [score:3]
Expression of gga-miR-375 in the liver of ALV-J infected chickens. [score:3]
Together, these data show that the ALV-J virus may inhibit gga-miR-375 thus blocking the Hippo pathway and facilitate tumour progression. [score:3]
These results collectively demonstrate that gga-miR-375 may inhibit cell proliferation and invasion by increasing apoptosis under serum starvation. [score:3]
This study was undertaken to explore the roles of gga-miR-375 in chickens with respect to tumour development and progression induced by ALV-J infection. [score:2]
To test whether the predicted gga-miR-375 -binding sites in the 3′-UTR of YAP1 mRNA were responsible for its regulatory role, the 3′-UTR region of YAP1 was cloned downstream of a luciferase reporter gene (YAP1-3′UTR-wildtype), and co -transfected DF-1 cells with gga-miR-375 precursor, miR-NC, or NT cells. [score:2]
miR-375 was originally reported in pancreatic islets of humans and mice to regulate insulin secretion in isolated pancreatic cells [23]. [score:2]
In hepatocellular carcinoma (HCC) research, miR-375 was found as an important regulator of the yes -associated protein (YAP) oncogene with a potential therapeutic role in HCC treatment [27]. [score:2]
0090878.g003 Figure 3The cells transfected with gga-miR-375, miR-NC, or mock were subjected to DAPI and Annexin V-FITC/PI staining. [score:1]
The cells were transfected with either gga-miR-375 (gga-miR-375) or negative control oligonucleotides gga-miR-NC (miR-NC), and then cultured for various periods of time (24, 48, or 72 hours). [score:1]
The cells transfected with gga-miR-375, miR-NC, or mock were subjected to DAPI and Annexin V-FITC/PI staining. [score:1]
gga-miR-375 promotes serum starvation induced apoptosis. [score:1]
The control RNA duplex (named gga-miR-NC; sense strand: UUCUCCGAACGUGUCACGUTT) was nonhomologous to any chicken genome sequence and used for gga-miR-375. [score:1]
We also intended to elucidate the molecular mechanisms underlying tumorigenesis and to evaluate whether gga-miR-375 expression levels could serve as a biomarker for diagnostic purposes. [score:1]
gga-miR-375 is most significantly associated with ALV-J infected liver tissue, as determined by significance analysis of microarrays. [score:1]
Alignment of YAP1-3′UTR, gga-miR-375, and MUT-3′UTR, where the complementary site for the seed region of gga-miR-375 is indicated. [score:1]
The gga-miR-375 differs from homo sapiens miR-375 and rattus norvegicus miR-375 by a single base (Figure 4A). [score:1]
The analysis of Annexin V-FITC/PI staining confirmed the gga-miR-375 increased serum starvation induced apoptosis from 54.2% to 36.6% (Figure 3C). [score:1]
Approximately 24 hours after transfection with gga-miR-375 or negative control oligonucleotides gga-miR-NC (miR-NC), DF-1 cells (1.0 × 10 [5] per millilitre) were seeded, respectively, into a 96-well plate and incubated for another 24, 48, or 72 hours. [score:1]
gga-miR-375 represses YAP1 protein production through 3′-UTR binding. [score:1]
About 24 hours after transfection with gga-miR-375 or miR-NC, DF-1 cells (1.0 × 10 [5] per millilitre) were seeded respectively into a 6-well plate and incubated for another 24, 48, or 72 hours under serum starvation; a blank control was also used. [score:1]
CHO cells were co -transfected with YAP1-3′UTR-wt with either gga-miR-375 or miR-NC (left), and YAP1-3′UTR-mut with either gga-miR-375 or miR-NC (right). [score:1]
gga-miR-375 promoted serum starvation induced apoptosis. [score:1]
Approximately 24 hours after transfection with gga-miR-375 or mir-NC, 1,000 transfected DF-1 cells were seeded in 6-well plates and maintained in DMEM containing 10% FBS for 2 weeks. [score:1]
Although there is no available ALV cancer cell line, transfection with synthetic gga-miR-375 oligonucleotides as well as other approaches designed to increase endogenous gga-miR-375 together with follow-up tests of long term animal infection are needed in further studies. [score:1]
The sequences of gga-miR-375, hsa-miR-375 and rno-miR-3375 (MI0003705, MI0000783, MI0006140) described in this paper have been deposited in miRBase (http://www. [score:1]
This also suggests that gga-miR-375 in chickens and miR-375 in humans are consistent on the function [40], [43], implicating mechanisms in different species and cancer types may reveal many similarities. [score:1]
At 48 or 72 hours after transfection with gga-miR-375 or miR-NC, DF-1 or CHO cells were subjected to Western blot analysis as described previously [42]. [score:1]
0090878.g004 Figure 4(A) Differences in gga-miR-375, homo sapiens miR-375, and rattus norvegicus miR-375. [score:1]
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2
[+] score: 61
The expression patterns of gga-miR-1a and gga-miR-21 were similar in the different developmental stages; relatively lower expression was observed from 42-d to 110-d compared with 162-d and increased dramatically to peak in 162-d. However, the expression dynamics of gga-miR-26a were different; the highest expression level was found in the 42-d ovary, then decreased from 70-d to 110-d, and finally increased in the 162-d ovary although still lower than 42-d. The expression of gga-miR-137 and gga-miR-375 in ovary decreased significantly from 42-d to 162-d. Figure 4 Expression patterns of gga-miR-1a, gga-miR-21, gga-miR-26a, gga-miR-137 and gga-miR-375 in different developmental stages of ovary and in different sized follicle in chicken by qRT-PCR assays. [score:13]
The expression patterns of gga-miR-1a and gga-miR-21 were similar in the different developmental stages; relatively lower expression was observed from 42-d to 110-d compared with 162-d and increased dramatically to peak in 162-d. However, the expression dynamics of gga-miR-26a were different; the highest expression level was found in the 42-d ovary, then decreased from 70-d to 110-d, and finally increased in the 162-d ovary although still lower than 42-d. The expression of gga-miR-137 and gga-miR-375 in ovary decreased significantly from 42-d to 162-d. Figure 4 Expression patterns of gga-miR-1a, gga-miR-21, gga-miR-26a, gga-miR-137 and gga-miR-375 in different developmental stages of ovary and in different sized follicle in chicken by qRT-PCR assays. [score:13]
Among the up-regulated miRNAs, gga-miR-1a has the largest fold-change (6.405-fold), while gga-miR-375 has the largest fold-change (11.345-fold) among the down-regulated miRNAs. [score:7]
Study has shown that overexpression of miR-375 suppressed glucose -induced insulin secretion, and conversely, inhibition of endogenous miR-375 function enhanced insulin secretion [61]. [score:7]
In this study, gga-miR-375 is the largest down-regulated by 11-fold in the mature ovary and 191 putative target genes were involved in insulin signaling pathway. [score:6]
To validate the Illumina small RNA deep sequencing data, five differentially expressed miRNAs (gga-miR-1a, gga-miR-21, gga-miR-26a, gga-miR-137 and gga-miR-375) were selected, and their expression levels were quantified using real-time quantitative RT-PCR (qRT-PCR). [score:5]
Among the down-regulated miRNAs, gga-miR-375 had the highest fold-change with 11.345-fold, followed by gga-miR-217, gga-miR-458b, gga-miR-449c and gga-miR-124a with more than 6-fold (Additional file 1: Table S4). [score:4]
To further characterize the functionality of these differentially expressed miRNAs identified from the chicken ovary, the expression levels of gga-miR-1a, gga-miR-21, gga-miR-26a, gga-miR-137 and gga-miR-375 were further examined in ovary tissues from 42-, 70-, 90-, 110- and 162-day-old White Leghorn hens (n =3), as well as in follicles isolated from ovaries of 162-day-old White Leghorn hens, namely, a large white follicle (LW, diameter =2-4 mm), small yellow follicle (SF, diameter =6-8 mm), F6 (diameter =12-14 mm), F4 (diameter =22-24 mm), F2 (diameter =30-31 mm) and F1 (diameter =34 mm) follicles. [score:3]
The expression of gga-miR-375 was relatively lower in LW and SF follicles, increased most in the F6-F2 follicles and then declined in the F1 stage, suggesting an important role of gga-miR-26a and gga-miR-137 in hierarchy maintenance of follicles in chicken. [score:3]
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3
[+] score: 30
Target genes of 14 differentially expressed miRNAs (gga-miR-122-5p, gga-miR-193a-5p, gga-miR-194, gga-miR-215-5p, gga-miR-217-3p, gga-miR-375, gga-miR-1559-3p, gga-miR-1769-3p, gga-miR-1788-3p, gga-miR-1798-5p, gga-miR-2954, gga-miR-3525, and gga-miR-3531-3p) were predicted by using the miRDB and TargetScan programs. [score:7]
Myotrophin (MTPN), a previously validated target of miR-375 (45), which promotes dimerization of NF-κB subunits (46) and regulates NF-κB transcription factor activity. [score:4]
miR-375 is a key regulator of epithelial properties that are necessary for securing epithelium-immune system cross talk (43) and much less expression in the colons of patients with active ulcerative colitis (44). [score:4]
A total of 14 differentially expressed miRNAs were detected (gga-miR-2954, gga-miR-215-3p, gga-miR-1798-5p, gga-miR-194, gga-miR-217-3p, gga-miR-1769-3p, gga-miR-1788-3p, gga-miR-1559-3p, gga-miR-3531-3p, gga-miR-215-5p, gga-miR-3525, gga-miR-193a-5p, gga-miR-122-5p, and gga-miR-375). [score:3]
At 5 days post S. Typhimurium challenge, expression of gga-miR-193a-5p and gga-miR-375 were not significantly changed in all treatment groups. [score:3]
LITAF is another target gene of human has-miR-375. [score:3]
To confirm and quantify the differential expression, five miRNAs (gga-miR-215-5p, gga-miR-3525, gga-miR-193a-5p, gga-miR-122-5p, and gga-miR-375) were randomly selected and validated by RT-PCR with six replicates of cecal samples in each group (Figure 3). [score:3]
Gga-miR-193a-5p decreased in the S and SP groups (0.438- and 0.432-fold change, respectively) and was not significantly changed in the P group, while gga-miR-215-5p and gga-miR-375 were not significantly changed among different treatment groups. [score:1]
Gga-miR-375 decreased in the S and SP group (0.231- and 0.115-fold change) and increased in the P group (1.324 fold change). [score:1]
On the other hand, miR-215-3p, miR-194, miR-217-3p, miR-215-3p, miR-193a-5p, miR-122-5p, and miR-375 are more conservative miRNAs in vertebrate animal species. [score:1]
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4
[+] score: 11
Seventeen miRNAs with different expression levels were selected randomly; one was not significantly expressed (miR-1786), the others are sixteen SDE miRNAs including six up-regulated (miR-375, -3523, -125b-5p, -130b-5p, -456-3p, and -460a-5p), and ten down-regulated miRNAs (miR-146b-5p, -24-3p, -451, -126-5p, -2188-5p, -33-3p, -22-3p, -148a-3p, -21-5p, and -10a-5p) (Fig. 4). [score:11]
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5
[+] score: 9
As illustrated in Fig 3, 7 miRNAs including miR-29c, miR-217, miR-375, miR-215, miR-19b, miR-133a and let-7a had relatively low and stable expression levels (P < 0.05) in early period, and increased significantly (P < 0.01) from 12 to 13 weeks when the gonads entered into rapid development. [score:4]
Of the 9 members, miR-217 and miR-375 are regulators of chicken ovary maturity [29]. [score:2]
miR-375 and let-7a also play roles in regulation of insulin sensitivity and glucose metabolism [41, 42, 43]. [score:2]
According to previous reports and our sequencing results, 9 miRNAs, including miR-29c-3p, miR-375, miR-215-5p, miR-9-5p, miR-19b-3p, miR-133a-3p, let-7a, miR-217-5p and miR-155 were determined as candidates. [score:1]
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[+] score: 7
Four up-regulated miRNAs (gga-miR-221, gga-miR-30b, gga-miR-30c and gga-miR-215) and 1 down-regulated miRNA (gga-miR-375) in RSS chicken were chosen randomly to validate the Solexa sequencing results using RT-qPCR. [score:7]
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7
[+] score: 5
To date, several miRNAs, including gga-miR-221, gga-miR-222, gga-miR-23b, gga-miR-375, gga-miR-125b, gga-miR-1650, gga-miR-193a, gga-miR-193b, gga-let-7b, gga-let-7i, gga-miR-458, gga-miR-1456, gga-miR-1704, gga-miR-1777, gga-miR-1790, and gga-miR-2127, have been reported to be associated with tumorigenesis and the aberrant expression of the retrovirus, ALV-J (Li et al., 2012; Wang et al., 2013a, b; Li H. et al., 2014; Dai et al., 2015; Li et al., 2015). [score:3]
In addition, gga-miR-221, gga-miR-222, and gga-miR-375 have been shown to play a pivotal role in tumorigenesis after an ALV-J infection (Li H. et al., 2014; Dai et al., 2015). [score:1]
Gga-miR-375 plays a key role in tumorigenesis post subgroup J avian leukosis virus infection. [score:1]
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8
[+] score: 4
Other miRNAs from this paper: hsa-mir-375, dre-mir-375-1, dre-mir-375-2, hsa-mir-485
Moreover, a recent study of a related miRNA gene (miR-375) has been recently shown to be selectively expressed in pancreatic islets and has been implicated both in the development of islets and the function of mature pancreatic beta cells [81]. [score:4]
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9
[+] score: 4
Interestingly, in silico studies have shown that LIX1 has a double-stranded RNA -binding domain, suggesting that it could be involved in mRNA or micro -RNA processing [10] and it has been shown that miR-506 and miR-375 regulate YAP1 expression [30, 31]. [score:4]
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10
[+] score: 3
In poultry, Li et al. [27], have reported gga-miR-375 may function as a tumor suppressor in infection of ALV-J. MiR-221 and miR-222, are examples of miRNA that is highly conserved among chordates and is located on the X chromosome in humans, rats, and mice [28]. [score:3]
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11
[+] score: 1
miR-375 is found to participate in the process of ovary maturation in chicken [42]. [score:1]
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12
[+] score: 1
Three miRNAs (gga-miR-135a, gga-miR-375, and gga-miR-429) were uniquely detected in MDV-infected samples, and three miRNAs, (gga-miR-1728-3p, gga-miR-1729*, and gga-miR-3530) were uniquely detected in non-infected samples. [score:1]
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13
[+] score: 1
Among them, moreover, 26 miRNAs (including 10 known miRNAs of miR-122, miR-1329-3p, miR-1587, miR-1736-3p, miR-1769-3p, miR-1769-5p, miR-1773-5p, miR-205a, miR-31 and miR-375) were found in all four comparisons (Table 3). [score:1]
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14
[+] score: 1
gga-miR-375 plays a key role in tumorigenesis post subgroup J avian leukosis virus infection. [score:1]
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15
[+] score: 1
Poy M. N. Hausser J. Trajkovski M. Braun M. Collins S. Rorsman P. Zavolan M. Stoffel M. miR-375 maintains normal pancreatic α- and beta-cell massProc. [score:1]
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