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6 publications mentioning mmu-mir-668

Open access articles that are associated with the species Mus musculus and mention the gene name mir-668. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 87
Expression of miRNAs in whole blood, serum and WBCs following LTA injection was quantified using real-time RT-PCR to verify selected up-regulated miRNA targets with at least 2-fold increase in expression (miR-451, miR-668, miR-1902, and miR-1904). [score:10]
First, LPS is a stronger stimulator than LTA to induce the expression of circulating miRNAs, considering the up-regulated 8 miRNAs of the whole blood showed approximately 5- to 12-fold increase in expression 6 h after 100 and 1000 μg LPS injection, these four miRNAs (miR-451, miR-668, miR-1902, and miR-1904) had a less prominent 2- to 6-fold increase upon LTA treatment. [score:8]
The up-regulated miRNA targets more than double of those of the control is shown in Table 1. There were 4 miRNAs (miR-451, miR-668, miR-1902, and miR-1904) showed significantly increased expression in the whole blood of mice exposed to LTA originated from different Gram -positive bacteria. [score:8]
In contrast to the expression of miR-451, miR-1902, miR-1904, but not miR-668, in serum of C57BL/6 at 6 h after exposure to 100 μg of LTA, no significantly up-regulation of these 4 miRNA targets was detected in the WBCs after LTA injection, when compared with those in C57BL/6 receiving PBS injection (Figure 4A). [score:7]
Using real-time RT-PCR, the expression of the up-regulated miR-451, miR-668, miR-1902 and miR-1904 was detected in the serum at 6 h following injection of 10, 100, 1000 μg of LTA; mice were killed at the indicated survival times (2, 6, 24, and 72 h) following 100 μg of LTA injection. [score:6]
Using real-time RT-PCR, the expression of the up-regulated miR-451, miR-668, miR-1902 and miR-1904 identified using miRNA microarray was detected in the whole blood at 6 h following injection of 10, 100, 1000 μg of LTA; mice were killed at the indicated survival times (2, 6, 24, and 72 h) following 100 μg of LTA injection. [score:6]
Up-regulation miR-668 in the peripheral blood of mice exposed to mainstream smoking had been found to reflect the dynamic pathological changes in smoking-related interstitial fibrosis [18], but so far there was no linkage of miR-668 to any infectious disease had been reported in the literature. [score:6]
In Tlr2 [−/−] mice, significantly higher expression of miR-451, miR-668, miR-1902, and miR-1904 in the serum were observed following LTA injection with around 6- to 10-fold of expression against that from the Tlr2 [−/−] mice injected with PBS (Figure 4B). [score:5]
In this study, we demonstrated that expression of miR-451, miR-668, miR-1902, and miR-1904 is significantly altered in the whole blood and serum of mice after exposure to LTA in a dose- and time -dependent fashion. [score:3]
Upon 100 ug of LTA injection, significantly increased expression around 2- to 3-fold was observed in miR-451, miR-1902, and miR-1904, but not in miR-668 (Figure 2A). [score:3]
However, in this study, higher expression of miR-451, miR-668, miR-1902, and miR-1904 in the serum were observed in Tlr2 [−/−] against C57BL/6 mice following LTA injection. [score:3]
When exposed to 1000 ug of LTA, all these four miRNAs (miR-451, miR-668, miR-1902, and miR-1904) had a significant expression around 3- to 6-fold than the control (Figure 2A). [score:3]
Notably, miR-451 was both induced by LPS and LTA treatment in this study and the expression of miR-668 was significantly induced in the whole blood and in the serum by the high dose of LTA in 1000 ug concentration, but not by lower to medium dose of LTA in 10 and 100 ug concentrations. [score:3]
Figure 5 Expression of miR-451, miR-668, miR-1902, and miR-1904 of serum from C57BL/6 mice using real-time RT-PCR experiments 6 h after exposure to 10, 100, and 1000 μg LPS from Escherichia coli serotype 026:B6; *, P < 0.05 vs. [score:3]
Figure 4 Expression of miR-451, miR-668, miR-1902, and miR-1904 of serum and WBCs from C57BL/6 and Tlr2 [−/− ] mice from real-time RT-PCR experiments 6 h after exposure to 100 μg of LTA or phosphate-buffered saline (PBS); **, P < 0.01 vs. [score:3]
In the whole blood, following injection with 10 μg of LTA, there was significantly increased expression in miR-1902, but not in miR-451, miR-668 or miR-1904 (Figure 2A). [score:3]
Whether the higher expression of miR-451, miR-668, miR-1902, and miR-1904 in the serum upon LTA stimulation is due to lack of the repressed targets of negative feedback loop in Tlr2 [−/−] mice is speculated but lack of evidence so far and required further investigation and validation. [score:3]
A significant increase of 4 miRNAs (miR-451, miR-668, miR-1902, and miR-1904) was observed in the whole blood and the serum in a dose- and time -dependent fashion following LTA injection. [score:1]
Therefore, the use of miR-451 or miR-668 as a biomarker to differentiate the exposure to LPS or LTA is not suitable. [score:1]
To investigate that whether LPS originating from Gram -negative bacteria Escherichia coli serotype 026:B6 (L3755) induces expression of miR-451, miR-668, miR-1902, and miR-1904, the serum was obtained for real-time PCR at 6 h following intraperitoneal injections of 10, 100, or 1000 μg LPS. [score:1]
Induction of miR-451, miR-1902, and miR-19042, but not miR-668, was evident at 6 h following injection with 100 ug of LTA (Figure 2B). [score:1]
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2
[+] score: 14
Other miRNAs from this paper: mmu-mir-1a-1, mmu-mir-127, mmu-mir-134, mmu-mir-136, mmu-mir-154, mmu-mir-181a-2, mmu-mir-143, mmu-mir-196a-1, mmu-mir-196a-2, mmu-mir-21a, rno-mir-329, mmu-mir-329, mmu-mir-1a-2, mmu-mir-181a-1, mmu-mir-181b-1, mmu-mir-181c, mmu-mir-375, mmu-mir-379, mmu-mir-181b-2, rno-mir-21, rno-mir-127, rno-mir-134, rno-mir-136, rno-mir-143, rno-mir-154, rno-mir-181c, rno-mir-181a-2, rno-mir-181b-1, rno-mir-181b-2, rno-mir-196a, rno-mir-181a-1, mmu-mir-196b, rno-mir-196b-1, mmu-mir-412, mmu-mir-370, oar-mir-431, oar-mir-127, oar-mir-432, oar-mir-136, mmu-mir-431, mmu-mir-433, rno-mir-431, rno-mir-433, ssc-mir-181b-2, ssc-mir-181c, ssc-mir-136, ssc-mir-196a-2, ssc-mir-21, rno-mir-370, rno-mir-412, rno-mir-1, mmu-mir-485, mmu-mir-541, rno-mir-541, rno-mir-493, rno-mir-379, rno-mir-485, bta-mir-21, bta-mir-181a-2, bta-mir-127, bta-mir-181b-2, bta-mir-181c, mmu-mir-181d, mmu-mir-493, rno-mir-181d, rno-mir-196c, rno-mir-375, mmu-mir-1b, bta-mir-1-2, bta-mir-1-1, bta-mir-134, bta-mir-136, bta-mir-143, bta-mir-154a, bta-mir-181d, bta-mir-196a-2, bta-mir-196a-1, bta-mir-196b, bta-mir-329a, bta-mir-329b, bta-mir-370, bta-mir-375, bta-mir-379, bta-mir-412, bta-mir-431, bta-mir-432, bta-mir-433, bta-mir-485, bta-mir-493, bta-mir-541, bta-mir-181a-1, bta-mir-181b-1, ssc-mir-1, ssc-mir-181a-1, mmu-mir-432, rno-mir-668, ssc-mir-143, ssc-mir-181a-2, ssc-mir-181b-1, ssc-mir-181d, ssc-mir-196b-1, ssc-mir-127, ssc-mir-432, oar-mir-21, oar-mir-181a-1, oar-mir-493, oar-mir-433, oar-mir-370, oar-mir-379, oar-mir-329b, oar-mir-329a, oar-mir-134, oar-mir-668, oar-mir-485, oar-mir-154a, oar-mir-154b, oar-mir-541, oar-mir-412, mmu-mir-21b, mmu-mir-21c, ssc-mir-196a-1, ssc-mir-196b-2, ssc-mir-370, ssc-mir-493, bta-mir-154c, bta-mir-154b, oar-mir-143, oar-mir-181a-2, chi-mir-1, chi-mir-127, chi-mir-134, chi-mir-136, chi-mir-143, chi-mir-154a, chi-mir-154b, chi-mir-181b, chi-mir-181c, chi-mir-181d, chi-mir-196a, chi-mir-196b, chi-mir-21, chi-mir-329a, chi-mir-329b, chi-mir-379, chi-mir-412, chi-mir-432, chi-mir-433, chi-mir-485, chi-mir-493, rno-mir-196b-2, bta-mir-668, ssc-mir-375
Because the genomes of sheep and goat are incomplete, we located the 21 co-expressed miRNAs on the completed cattle genome and found that, apart from the miR-668 family which is not expressed in cattle, the other 20 families were all clustered in the 66039214–67786949 region of bovine chromosome 21. [score:5]
It is possible that mir-368 and mir-668 may be related to the development and normal apoptosis of skin hair follicle and, therefore, these miRNAs may be involved in regulating the development of the skin and hair follicle. [score:4]
The over -expression of mir-668 increased the activity of β-galactosidase associated with aging, suggesting that mir-668 is an aging -induced miRNA [26]. [score:3]
Taking advantage of the close homology between them, we compared 21 miRNA families in sheep and goat (Table S2), and found that the miR-368 and miR-668 families were expressed only in sheep and goat and not in cattle, horse, and pig. [score:2]
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3
[+] score: 7
microRNA Gene targets from axonal guidance pathwaylet-7a-5p (and other miRNAs w/seed GAGGUAG) miR-17-5p (and other miRNAs w/seed AAAGUGC) miR-320b (and other miRNAs w/seed AAAGCUG) miR-668-3p (and other miRNAs w/seed GUCACUC) miR-767 (and other miRNAs w/seed GCACCAU) ACTR2, ADAM12, ADAM9, ADAMTS1, ADAMTS5, ADAMTS8, AKT2, AKT3, ARHGEF11, ARHGEF15, ARHGEF7, ARPC5, BMP2, BMP3, BMP6, CFL2, CRK, CRKL, DCC, DPYSL2, DPYSL5, EFNB1, EFNB2, ENPEP, EPHA3, EPHA4, EPHA5, EPHA7, EPHB4, FRS2, FZD3, FZD4, FZD7, GAB1, GNAI1, GNAL, GNAT1, GNAZ, GNB5, GNG5, GRB2, IGF1, IRS2, ITGA4, LIMK1, LIMK2, LINGO1, MAPK1, MBTPS2, MMP11, MMP2, MYL12A, NFAT5, NFATC3, NGF, NRAS, NRP1, NRP2, NTN1, NTRK3, PAK1, PAK7, PAPPA, PDGFB, PFN2, PIK3R1, PLCB1, PLCG1, PLXNA1, PLXNC1, PLXND1, PPP3R1, PRKACB, PRKAG2, PRKAR1A, PRKCB, RAC1, RAP1A, RASA1, SEMA3A, SEMA3F, SEMA4B, SEMA4C, SEMA4F, SEMA4G, SEMA5A, SEMA7A, SHANK2, SOS1, SRGAP1, SRGAP3, TUBA8, UNC5A, VASP, VEGFA, WASL, WNT1 Furthermore, three miRNAs were common between Con vs. [score:3]
microRNA Gene targets from axonal guidance pathwaylet-7a-5p (and other miRNAs w/seed GAGGUAG) miR-17-5p (and other miRNAs w/seed AAAGUGC) miR-320b (and other miRNAs w/seed AAAGCUG) miR-668-3p (and other miRNAs w/seed GUCACUC) miR-767 (and other miRNAs w/seed GCACCAU) ACTR2, ADAM12, ADAM9, ADAMTS1, ADAMTS5, ADAMTS8, AKT2, AKT3, ARHGEF11, ARHGEF15, ARHGEF7, ARPC5, BMP2, BMP3, BMP6, CFL2, CRK, CRKL, DCC, DPYSL2, DPYSL5, EFNB1, EFNB2, ENPEP, EPHA3, EPHA4, EPHA5, EPHA7, EPHB4, FRS2, FZD3, FZD4, FZD7, GAB1, GNAI1, GNAL, GNAT1, GNAZ, GNB5, GNG5, GRB2, IGF1, IRS2, ITGA4, LIMK1, LIMK2, LINGO1, MAPK1, MBTPS2, MMP11, MMP2, MYL12A, NFAT5, NFATC3, NGF, NRAS, NRP1, NRP2, NTN1, NTRK3, PAK1, PAK7, PAPPA, PDGFB, PFN2, PIK3R1, PLCB1, PLCG1, PLXNA1, PLXNC1, PLXND1, PPP3R1, PRKACB, PRKAG2, PRKAR1A, PRKCB, RAC1, RAP1A, RASA1, SEMA3A, SEMA3F, SEMA4B, SEMA4C, SEMA4F, SEMA4G, SEMA5A, SEMA7A, SHANK2, SOS1, SRGAP1, SRGAP3, TUBA8, UNC5A, VASP, VEGFA, WASL, WNT1Furthermore, three miRNAs were common between Con vs. [score:3]
A comparison revealed that seven miRNAs (let7 [*], let-7a-5p, miR-17 [*], miR-320, miR-668, miR-767, miR3068-3p), were commonly deregulated in NSCs from diabetic pregnancy, hypoxia and HG groups when compared to the control (Table 5). [score:1]
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4
[+] score: 6
Previously, we had demonstrated a dose- and time -dependent up-regulation of 8 (let-7d, miR-15b, miR-16, miR-25, miR-92a, miR-103, miR-107, and miR-451) and 4 (miR-451, miR-668, miR-1902, and miR-1904) circulating miRNA targets in mice following injection of LPS [11] and LTA [12], respectively. [score:6]
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5
[+] score: 1
In comparison to Mirg, the longest Meg9 clone has nine additional upstream exons and contains four more microRNAs: miR-382, miR-134, miR-668, and miR-485. [score:1]
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6
[+] score: 1
HNC-TICs with SB treatment resulted increase in the levels of various miRNA, including miR-363-5p, miR-4443, miR-4448, miR-4454, miR-720, miR-668, and miR-494 (Figure 4A). [score:1]
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