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15 publications mentioning bta-mir-320a-2

Open access articles that are associated with the species Bos taurus and mention the gene name mir-320a-2. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

[+] score: 74
Interestingly, qRT-PCR analysis of a PGR transcript targeted by two miRNAs revealed a significant upregulation of its expression in all four biological replicates for each treatment, with fold changes of 2.17 and 4.18 for miR-122 (P-value = 0.002) and miR-320a (P-value = 0.004), respectively. [score:8]
To assess the impact of miRNA supplementation on gene expression changes in the BEECs, we used TargetScan [3] (Lewis et al., 2005; Agarwal et al., 2015) to search for target genes of miR-22, miR-122, and miR-320a that could have important roles in fetal-mother communication. [score:7]
Computational prediction revealed that all three miRNAs (miR-22, miR-122, and miR-320a) collectively target PGR, and further functional analysis showed a trend of upregulation of the PGR transcript following supplementation with these miRNAs individually. [score:6]
Expression analysis revealed upregulation of all three miRNAs in the media of females, with overall fold changes of 1.77, 1.86, and 1.86 for miR-22 (P < 0.05), miR-122 (P < 0.05), and miR-320a (P < 0.05), respectively (Figure 2). [score:6]
A study by Feng et al. (2015) identified that presence of miR-320 in the human follicular fluid is decreased in patients using intracytoplasmic sperm injection to conceive, and knockdown of miR-320 in mouse oocytes negatively affects developmental potential of embryos through inhibition of the Wnt signaling pathway. [score:5]
Synthetic mimics for the miRNAs miR-22, miR-122, and miR-320a (Qiagen) — specific to those found to be differentially expressed between male and female embryos — were supplemented to BEECs to assess whether these miRNAs modulate maternal gene expression. [score:5]
Additionally, lipofectamine -transfected cells exhibited upregulation of PGR gene expression for all three miRNA treatments, with fold changes of 1.51 (miR-22), 1.50 (miR-122), and 1.41 (miR-320a) when compared with a lipofectamine-only treated control (Supplementary Table 5). [score:5]
A total of 68 miRNAs were profiled using Firefly [®] technology, of which miRNAs miR-122 (P-value = 0.048), miR-16 (P-value = 0.042), miR-30b (P-value = 0.029), miR-320a (P-value = 0.042), miR-15b (P-value = 0.089), miR-16-2 (P-value = 0.061), miR-17 (P-value = 0.072), and miR-22 (P-value = 0.069) were found to be upregulated in female-conditioned embryo media (Figure 1). [score:4]
Fold difference of expression for miR-22 (P < 0.05), miR-122 (P < 0.05), and miR-320a (P < 0.05) in female versus male-conditioned culture medium. [score:3]
Angiogenesis, a process necessary for embryo viability, is inhibited by miR-320 in rat myocardial microvascular endothelial cells (Wang et al., 2009). [score:3]
For the validation of differentially expressed miRNAs detected by Firefly [®] particle technology, three miRNAs (miR-22, miR-122, and miR-320a) were chosen for further analysis by qRT-PCR. [score:3]
FIGURE 4Progesterone receptor (PGR) gene expression is increased following miRNA mimic (50 nM) supplementation to bovine endometrial epithelial cells for miR-22 (P < 0.08), miR-122 (P < 0.05), and miR-320a (P < 0.05) compared with untreated control for four biological replicates. [score:2]
A subset of miRNAs found to be differentially expressed in media of female embryos compared to male embryos (miR-122, miR-22, and miR-320a) was selected for validation using qRT-PCR analysis. [score:2]
The observation that miR-122 decreases from hours 0 to 22 of maturation in bovine oocytes (Abd El Naby et al., 2016) and the detection of miR-320a in human embryo culture medium at the cleavage, morula, and blastocyst stages (Capalbo et al., 2016) indicate these miRNAs could serve as continuous markers of embryo developmental checkpoints. [score:2]
MicroRNA-320 expression in myocardial microvascular endothelial cells and its relationship with insulin-like growth factor-1 in type 2 diabetic rats. [score:2]
MiR-122 (P < 0.05) and miR-320a (P < 0.05) were significantly higher in expression compared to control non-supplemented cells, with fold changes of 28.61 and 44.96, respectively. [score:2]
FIGURE 3Increased expression of miRNAs in Bovine Endometrial Epithelial Cells following supplementation with 50 nM miRNA mimic for miR-22 (P > 0.05), miR-122 (P < 0.05), and miR-320a (P < 0.05) compared with untreated control for four biological replicates. [score:2]
Figure 4 shows the fold difference in expression of PGR in endometrial cells supplemented with miRNAs miR-122, miR-22, and miR-320a compared to control cells. [score:2]
To test whether embryonic miRNAs are taken up by maternal endometrial cells, synthetic miRNA mimics corresponding to miR-22, miR-122, and miR-320a were supplemented to primary endometrial epithelial cells. [score:1]
Significant uptake of miR-122 and miR-320a, as well as a trend of uptake for miR-22, were observed in this study. [score:1]
The qRT-PCR for miR-122, miR-22, and miR-320a was performed on three biological replicates of media for embryos produced from new dams. [score:1]
miRNAs miR-122 (P-value = 0.048), miR-16 (P-value = 0.042), miR-30b (P-value = 0.029), miR-320a (P-value = 0.042), miR-15b (P-value = 0.089), miR-16-2 (P-value = 0.061), miR-17 (P-value = 0.072), and miR-22 (P-value = 0.069) were found to be significant. [score:1]
MiRNA-320 in the human follicular fluid is associated with embryo quality in vivo and affects mouse embryonic development in vitro. [score:1]
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[+] score: 32
Bioinformatic analysis using target genes of miR-21-5p, miR-26a, and miR-320a predicted that the molecular biological events with which the target genes of those miRNAs were associated are transcriptional regulation, protein phosphorylation, phosphate metabolic process, embryonic morphogenesis, tube development, and positive regulation of biosynthetic process. [score:8]
According to the results of our TargetScan analysis, a total of 1,617 bovine genes are predicted as the targets of significantly reduced miRNAs by DIP-treatment of BMEC (miR-21-5p, miR-26a, and miR-320a). [score:5]
These terms of biological process suggest that the down-regulations of miR-21-5p, miR-26a, and miR-320a in BMECs may have roles in the mammary gland differentiation and lactation of dairy cows. [score:4]
Intriguingly, according to the miRNA qPCR results, it is likely that most of cellular miRNAs including miR-21-5p, miR-26a, and miR-320a in BMEC culture are down-regulated after DIP treatment. [score:4]
The cellular expression of miR-21-5p, miR-25, miR-26a, miR-223, and miR-320a was lower in the DIP -treated BMECs than in the untreated BMECs (P = 0.005, = 0.059, = 0.016, = 0.054, and = 0.011, respectively), whereas that of the other miRNAs was not significantly changed by the treatment (Fig.   4a). [score:3]
Quantitative polymerase chain reaction (qPCR) results showed that the expressions of miR-21–5p (P = 0.005), miR-26a (P = 0.016), and miR-320a (P = 0.011) were lower in the DIP -treated cells than in the untreated cells. [score:3]
In the qPCR analyses of 18 miRNAs that were abundant in milk, the expressions of miR-21-5p, miR-26a, and miR-320a were significantly lower in the DIP -treated cells than in the untreated cells. [score:3]
Of those, miR-7b, miR-21-5p, miR-23-3p, miR-26a, miR-30a, miR-103, miR-107, miR-148a, miR-200c, and miR-320a were among the top 30 most abundant miRNAs in the milk. [score:1]
The top 20 miRNAs in the milk exosome were let-7b (12.7 %), miR-200c (10.9 %), miR-26a (8.8 %), let-7c (7.5 %), let-7a-5p (6.3 %), miR-30a-5p (3.1 %), miR-320a (2.7 %), miR-103 (2.5 %), miR-107 (2.2 %), let-7d (1.9 %), miR-23-3p (1.6 %), miR-191 (1.6 %), miR-23a (1.6 %), miR-20a (1.5 %), miR-1777b (1.5 %), miR-151-5p (1.4 %), miR-24-3p (1.3 %), miR-320b (1.3 %), miR-200b (1.2 %), and miR-141 (1.2 %) (Fig.   2). [score:1]
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[+] score: 27
According to the results of TargetScan analysis, totally 2743 bovine genes were predicted as the targets of c-miRNAs significantly down-regulated by grazing (miR-19b, miR-148a, miR-150, miR-221, miR-223 miR-320a, miR-361, and miR-486). [score:8]
Down-regulation of the miR-320a level is associated with up-regulation of the muscle-type phosphofructokinase gene and an increase in lactate, namely, enhancement of glycolysis. [score:7]
Of these c-miRNAs, circulation levels of miR-19b, miR-148a, miR-150, miR-221, miR-223, miR-320a, miR-361, and miR-486 were significantly down-regulated in the grazing cattle compared to housed cattle, whereas the miR-451 level was higher in the grazing than in the housed cattle. [score:3]
Expression of miR-320a is ubiquitously distributed in tissues of cattle [53]; however, its role remains unclear. [score:3]
The lower level of glycolysis-related miR-320a in circulation [54] might be associated with muscle phenotypic changes induced by grazing as shown previously [16]. [score:1]
In the present study, the miR-320a level in circulation was higher in the housed cattle than in the grazing cattle at 4 mo. [score:1]
Meanwhile, in the present study, circulating levels of miR-19b, miR-150, miR-223, and miR-320a were temporarily lower in the grazing cattle than in the housed, suggesting that there might be some stress on the grazing cattle. [score:1]
No exercise -induced changes in circulating miR-19b, miR-150, miR-320a, or miR-361 have ever been reported, which may indicate the specificity of changes in those miRNAs to the grazing movements of cattle. [score:1]
Grazing -induced miRNAs: miR-19b, miR-150, miR-223, miR-320a, miR-361. [score:1]
At 2 mo, the levels of miR-19b, miR-150, miR-223, miR-320a, and miR-361 in the grazing cattle were lower than in the housed cattle (P = 0.015, 0.020, 0.026, 0.023, and 0.089, respectively). [score:1]
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[+] score: 22
Among them, the more expressed let-7, miR-142, miR-196b and lower expressed miR-320a have been reported to regulate lipid metabolism. [score:6]
The expression level of 3 differentially expressed miRNAs, bta-miR-320a, bta-miR-874 and bta-miR-1247-3p, and a predicted novel miRNA chr4_8522, were randomly selected and verified by qRT-PCR. [score:5]
MiR-320 family have been found to promote adipocyte differentiation via inhibiting Runt-related transcription factor 2 (RUNX2) which is a key osteoblast-specific transcription factor that induce mesenchymal stem cells (MSCs) to differentiate into osteoblasts and suppress adipocytic differentiation 38. [score:4]
Interestingly, bta-miR-320a was also presented in the top ten high abundant miRNAs, and it exhibited a significantly differential expression pattern between Wagyu and Holstein cattle (P < 0.05). [score:3]
Another study also revealed that miR-320 regulates insulin resistance via insulin-PI3K signaling pathway 39. [score:2]
Taken together with our present result, it may be concluded that miR-320 plays an important role in adipogenesis and subsequent difference in fat deposition between Wagyu and Holstein cattle. [score:1]
Worthy of mention, miR-320a was in the top ten within the high abundant miRNAs, which implies that it may play an important role in beef adipogenesis. [score:1]
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[+] score: 18
In network 2, bta-miR-320a and -432 targeted adenosine monophosphate-activated protein kinase alpha 1 (PRKAA1) and bta-miR-199a-3p targeted PRKAA1/2, which are the master regulators in glucose and lipid metabolism [38]. [score:6]
Additionally, bta-miR-320a and -432 targeted peroxisome proliferator-activated receptor-gamma (PPARG) in network 5, and bta-miR-320a targeted peroxisome proliferator-activated receptor-alpha (PPARA) in network 8, respectively. [score:5]
Table S8 Predicted targets for bta-miR-320a. [score:3]
Further analyses revealed that 370 AF-specific miRNA target genes relating to lipid metabolism and adipogenesis were annotated with 28 bta-miR-154c GO terms (Table S6C), 28 bta-miR-199a-3p GO terms (Table S7C), 47 bta-miR-320a GO terms (Table S8C) and 15 bta-miR-432 GO terms (Table S9C), respectively. [score:3]
Bta-miR-320a comprised of 2 isoforms: miR-320a-1 located on bovine chromosome 8 from 70060384 to 70060465 and miR-320a-2 located on bovine chromosome 20 from 15213924 to 15214005 [33]. [score:1]
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[+] score: 15
Other miRNAs from this paper: bta-mir-29a, bta-let-7f-2, bta-mir-27a, bta-mir-99a, bta-mir-125a, bta-mir-181a-2, bta-mir-27b, bta-mir-10a, bta-mir-139, bta-mir-140, bta-mir-181b-2, bta-mir-487a, bta-let-7d, bta-mir-124a-1, bta-mir-181c, bta-mir-29b-2, bta-mir-29c, bta-mir-423, bta-let-7g, bta-mir-10b, bta-let-7a-1, bta-mir-487b, bta-let-7f-1, bta-mir-122, bta-let-7i, bta-mir-25, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-195, bta-mir-34a, bta-mir-1-2, bta-mir-1-1, bta-mir-124a-2, bta-mir-124b, bta-mir-133a-2, bta-mir-133a-1, bta-mir-133b, bta-mir-154a, bta-mir-181d, bta-mir-184, bta-mir-206, bta-mir-29d, bta-mir-335, bta-mir-33a, bta-mir-33b, bta-mir-486, bta-mir-495, bta-mir-95, bta-mir-9-1, bta-mir-9-2, bta-mir-29e, bta-mir-29b-1, bta-mir-1271, bta-mir-1249, bta-mir-181a-1, bta-mir-181b-1, bta-mir-2284i, bta-mir-2286, bta-mir-2300a, bta-mir-2300b, bta-mir-2284s, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2284d, bta-mir-2319a, bta-mir-2319b, bta-mir-2284n, bta-mir-2284g, bta-mir-2329-1, bta-mir-2329-2, bta-mir-2284p, bta-mir-2284u, bta-mir-2363-1, bta-mir-2363-2, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2396, bta-mir-2285c, bta-mir-2284q, bta-mir-2404-1, bta-mir-2284m, bta-mir-2284b, bta-mir-320b, bta-mir-2284r, bta-mir-2443, bta-mir-2284h, bta-mir-2450c, bta-mir-2450b, bta-mir-2450a, bta-mir-2404-2, bta-mir-2284o, bta-mir-2484, bta-mir-2284e, bta-mir-320a-1, bta-mir-2887-1, bta-mir-2887-2, bta-mir-2284w, bta-mir-3431, bta-mir-2284x, bta-mir-3432a-1, bta-mir-3432a-2, bta-mir-574, bta-mir-2284y-1, bta-mir-2284y-2, bta-mir-2284y-3, bta-mir-154c, bta-mir-154b, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-6526-1, bta-mir-6526-2, bta-mir-503, bta-mir-2284y-7, bta-mir-6526-3, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2284z-4, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-6536-1, bta-mir-2284aa-4, bta-mir-6536-2, bta-mir-2284z-2, bta-mir-133c, bta-mir-2284ab, bta-mir-2284ac, bta-mir-3432b, bta-mir-2450d
In addition, miR-206, miR-1, and miR-320 were up-regulated during MDSC differentiation; miR-495, miR-133b, and miR-487 were down-regulated in MDSC-D1 and upregulated in MDSC-D3. [score:10]
The results showed that miR-29a, miR-27a, and let-7i were highly expressed in MDSC-P; in contrast, miR-320, miR-1, and miR-206 were highly expressed in MDSC-D3. [score:5]
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[+] score: 13
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-17, hsa-mir-18a, hsa-mir-19a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-20a, hsa-mir-22, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, hsa-mir-27a, hsa-mir-29a, hsa-mir-30a, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-98, hsa-mir-99a, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-106a, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-10a, hsa-mir-10b, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-182, hsa-mir-181a-1, hsa-mir-221, hsa-let-7g, hsa-let-7i, hsa-mir-1-2, hsa-mir-15b, hsa-mir-27b, hsa-mir-30b, hsa-mir-130a, hsa-mir-152, hsa-mir-191, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-185, hsa-mir-193a, hsa-mir-320a, hsa-mir-200c, hsa-mir-1-1, hsa-mir-181b-2, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-99b, hsa-mir-130b, hsa-mir-30e, hsa-mir-363, hsa-mir-374a, hsa-mir-375, hsa-mir-378a, hsa-mir-148b, hsa-mir-331, hsa-mir-339, hsa-mir-423, hsa-mir-20b, hsa-mir-491, hsa-mir-193b, hsa-mir-181d, hsa-mir-92b, hsa-mir-320b-1, hsa-mir-320c-1, hsa-mir-320b-2, hsa-mir-378d-2, bta-mir-29a, bta-let-7f-2, bta-mir-148a, bta-mir-18a, bta-mir-20a, bta-mir-221, bta-mir-27a, bta-mir-30d, bta-mir-99a, bta-mir-181a-2, bta-mir-27b, bta-mir-30b, bta-mir-106a, bta-mir-10a, bta-mir-15b, bta-mir-181b-2, bta-mir-193a, bta-mir-20b, bta-mir-30e, bta-mir-92a-2, bta-mir-98, bta-let-7d, bta-mir-148b, bta-mir-17, bta-mir-181c, bta-mir-191, bta-mir-200c, bta-mir-22, bta-mir-29b-2, bta-mir-29c, bta-mir-423, bta-let-7g, bta-mir-10b, bta-mir-24-2, bta-mir-30a, bta-let-7a-1, bta-let-7f-1, bta-mir-30c, bta-let-7i, bta-mir-25, bta-mir-363, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-15a, bta-mir-19a, bta-mir-19b, bta-mir-331, bta-mir-374a, bta-mir-99b, hsa-mir-374b, hsa-mir-320d-1, hsa-mir-320c-2, hsa-mir-320d-2, bta-mir-1-2, bta-mir-1-1, bta-mir-130a, bta-mir-130b, bta-mir-152, bta-mir-181d, bta-mir-182, bta-mir-185, bta-mir-24-1, bta-mir-193b, bta-mir-29d, bta-mir-30f, bta-mir-339a, bta-mir-374b, bta-mir-375, bta-mir-378-1, bta-mir-491, bta-mir-92a-1, bta-mir-92b, bta-mir-9-1, bta-mir-9-2, bta-mir-29e, bta-mir-29b-1, bta-mir-181a-1, bta-mir-181b-1, bta-mir-320b, bta-mir-339b, bta-mir-19b-2, bta-mir-320a-1, bta-mir-193a-2, bta-mir-378-2, hsa-mir-378b, hsa-mir-320e, hsa-mir-378c, bta-mir-148c, hsa-mir-374c, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, hsa-mir-378j, bta-mir-378b, bta-mir-378c, bta-mir-378d, bta-mir-374c, bta-mir-148d
Meanwhile, miR-320 is able to inhibit HL-60 cell proliferation by suppressing receptor 1 (TfR-1; CD71) [72], and miR-181a was believed to act as an intrinsic antigen sensitivity “rheostat” during T cell development [73]. [score:6]
MiR-320, miR-181a, miR-30a-3p and let-7 were shown to be downregulated in colorectal cancer [74]. [score:4]
Notably, some miRNAs among the top 10 identified here have been reported to be related to immunity (miR-320, miR-181a, miR-30a-3p, let-7a, let-7f and let-7c) and development (miR-193a-3p, miR-378 and miR-191). [score:2]
The top 10 miRNAs were ssc-miR-193a-3p, ssc-miR-423-5p, ssc-miR-320, ssc-miR-181a, ssc-miR-30a-3p, ssc-miR-378, ssc-miR-191, ssc-let-7a, ssc-let-7f and ssc-let-7c. [score:1]
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[+] score: 5
Moreover the miRNAs were also clustered together based on their expression levels, for example, miRNAs bta-miR-320a, -194, -186 and -181a; having similar expression profiles formed a cluster. [score:5]
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[+] score: 3
Using this approach we identified five new orthologous miRNAs in cattle genome (cfa-mir-1842, cfa-mir-194, hsa-mir-1277, hsa-mir-1468, hsa-mir-320a) that were expressed in our deep sequencing data; two of those also had detectable miRNA* (cfa-mir-1842*, cfa-mir-194*) (Supplemental Table S1). [score:3]
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[+] score: 3
Other miRNAs from this paper: bta-let-7f-2, bta-mir-101-2, bta-mir-103-1, bta-mir-21, bta-mir-27a, bta-mir-30d, bta-mir-199a-1, bta-mir-27b, bta-mir-30b, bta-mir-107, bta-mir-140, bta-mir-30e, bta-let-7d, bta-mir-124a-1, bta-mir-199b, bta-mir-200a, bta-mir-200c, bta-let-7g, bta-mir-30a, bta-mir-200b, bta-let-7a-1, bta-let-7f-1, bta-mir-122, bta-mir-30c, bta-let-7i, bta-mir-25, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-1-2, bta-mir-1-1, bta-mir-101-1, bta-mir-124a-2, bta-mir-124b, bta-mir-133a-2, bta-mir-133a-1, bta-mir-143, bta-mir-152, bta-mir-154a, bta-mir-185, bta-mir-199a-2, bta-mir-206, bta-mir-30f, bta-mir-335, bta-mir-33a, bta-mir-33b, bta-mir-370, bta-mir-378-1, bta-mir-432, bta-mir-9-1, bta-mir-9-2, bta-mir-1224, bta-mir-376b, bta-mir-376d, bta-mir-376c, bta-mir-376a, bta-mir-1839, bta-mir-1185, bta-mir-2284i, bta-mir-2285a, bta-mir-2284s, bta-mir-2285d, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2285b-1, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-199c, bta-mir-2284p, bta-mir-2284u, bta-mir-2363-1, bta-mir-2363-2, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2285c, bta-mir-2284q, bta-mir-2284m, bta-mir-2284b, bta-mir-320b, bta-mir-2284r, bta-mir-2284h, bta-mir-2284o, bta-mir-2284e, bta-mir-320a-1, bta-mir-378-2, bta-mir-2284w, bta-mir-2284x, bta-mir-3432a-1, bta-mir-3432a-2, bta-mir-3604-1, bta-mir-3604-2, bta-mir-2284y-1, bta-mir-2285e-1, bta-mir-2285e-2, bta-mir-2285f-1, bta-mir-2285f-2, bta-mir-2285g-1, bta-mir-2285h, bta-mir-2285i, bta-mir-2285j-1, bta-mir-2285j-2, bta-mir-2285k-1, bta-mir-2285l, bta-mir-2285o-1, bta-mir-2285o-2, bta-mir-2285n-1, bta-mir-2285n-2, bta-mir-2285p, bta-mir-2285m-1, bta-mir-2285m-2, bta-mir-2284y-2, bta-mir-378b, bta-mir-2285n-3, bta-mir-2285n-4, bta-mir-2284y-3, bta-mir-154c, bta-mir-376e, bta-mir-154b, bta-mir-2285o-3, bta-mir-2285o-4, bta-mir-2285m-3, bta-mir-378c, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2285m-4, bta-mir-2285o-5, bta-mir-2285m-5, bta-mir-2285n-5, bta-mir-2285n-6, bta-mir-6526-1, bta-mir-6526-2, bta-mir-503, bta-mir-2284y-7, bta-mir-6526-3, bta-mir-2285n-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2285k-2, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2285k-3, bta-mir-2285k-4, bta-mir-2284z-4, bta-mir-2285k-5, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-6536-1, bta-mir-2284aa-4, bta-mir-6536-2, bta-mir-2285q, bta-mir-2285r, bta-mir-2285s, bta-mir-2285t, bta-mir-2285b-2, bta-mir-2285v, bta-mir-2284z-2, bta-mir-2285g-2, bta-mir-2285g-3, bta-mir-2285af-1, bta-mir-2285af-2, bta-mir-2285y, bta-mir-2285w, bta-mir-2285x, bta-mir-2285z, bta-mir-2285u, bta-mir-2285aa, bta-mir-2285ab, bta-mir-2284ab, bta-mir-2285ac, bta-mir-2285ad, bta-mir-2284ac, bta-mir-2285ae, bta-mir-378d, bta-mir-3432b, bta-mir-2285ag, bta-mir-2285ah, bta-mir-2285ai, bta-mir-2285aj, bta-mir-2285ak, bta-mir-2285al, bta-mir-2285am, bta-mir-2285ar, bta-mir-2285as-1, bta-mir-2285as-2, bta-mir-2285as-3, bta-mir-2285at-1, bta-mir-2285at-2, bta-mir-2285at-3, bta-mir-2285at-4, bta-mir-2285au, bta-mir-2285av, bta-mir-2285aw, bta-mir-2285ax-1, bta-mir-2285ax-2, bta-mir-2285ax-3, bta-mir-2285ay, bta-mir-2285az, bta-mir-2285an, bta-mir-2285ao-1, bta-mir-2285ao-2, bta-mir-2285ap, bta-mir-2285ao-3, bta-mir-2285aq-1, bta-mir-2285aq-2, bta-mir-2285ba-1, bta-mir-2285ba-2, bta-mir-2285bb, bta-mir-2285bc, bta-mir-2285bd, bta-mir-2285be, bta-mir-2285bf-1, bta-mir-2285bf-2, bta-mir-2285bf-3, bta-mir-2285bg, bta-mir-2285bh, bta-mir-2285bi-1, bta-mir-2285bi-2, bta-mir-2285bj-1, bta-mir-2285bj-2, bta-mir-2285bk, bta-mir-2285bl, bta-mir-2285bm, bta-mir-2285bn, bta-mir-2285bo, bta-mir-2285bp, bta-mir-2285bq, bta-mir-2285br, bta-mir-2285bs, bta-mir-2285bt, bta-mir-2285bu-1, bta-mir-2285bu-2, bta-mir-2285bv, bta-mir-2285bw, bta-mir-2285bx, bta-mir-2285by, bta-mir-2285bz, bta-mir-2285ca, bta-mir-2285cb, bta-mir-2285cc, bta-mir-2285cd, bta-mir-2285ce, bta-mir-2285cf, bta-mir-2285cg, bta-mir-2285ch, bta-mir-2285ci, bta-mir-2285cj, bta-mir-2285ck, bta-mir-2285cl, bta-mir-2285cm, bta-mir-2285cn, bta-mir-2285co, bta-mir-2285cp, bta-mir-2285cq, bta-mir-2285cr-1, bta-mir-2285cr-2, bta-mir-2285cs, bta-mir-2285ct, bta-mir-2285cu, bta-mir-2285cv-1, bta-mir-2285cv-2, bta-mir-2285cw-1, bta-mir-2285cw-2, bta-mir-2285cx, bta-mir-2285cy, bta-mir-2285cz, bta-mir-2285da, bta-mir-2285db, bta-mir-2285dc, bta-mir-2285dd, bta-mir-2285de, bta-mir-2285df, bta-mir-2285dg, bta-mir-2285dh, bta-mir-2285di, bta-mir-2285dj, bta-mir-2285dk, bta-mir-2285dl-1, bta-mir-2285dl-2, bta-mir-2285dm
The expression levels of the 15 miRNAs were determined, and the selected miRNAs included five reported tissue-specific miRNAs (bta-miR-9, miR-124, miR-122, miR-27, and miR-103), seven backfat-predominant miRNAs in fetal and/or adult bovine backfat libraries (let-7a, miR-140, miR-199a, miR-320, miR-2284x, miRn8, and miRn25) as well as three high-read miRNAs (miR-154, miR-1839, and miRn26) in backfat that were compared with the muscle library. [score:2]
In contrast, another seven miRNAs were found in six or more tissues, and the two most abundant miRNAs across the eight tissues were miRNA-199a and miRNA-320 (Figure 4A and Figure S3). [score:1]
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[+] score: 3
Variant expression followed two main patterns as seen in other studies [40] those miRNAs with a strong predominant isomiR, such as bta-miR-191 and bta-miR-103; and those miRNAs where there is no predominant isomiR, such as bta-miR-486 and bta-miR-320a. [score:3]
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[+] score: 3
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-16-1, hsa-mir-20a, hsa-mir-21, hsa-mir-22, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-27a, hsa-mir-31, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-101-1, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-16-2, hsa-mir-192, hsa-mir-199a-1, hsa-mir-30c-2, hsa-mir-199a-2, hsa-mir-223, hsa-let-7g, hsa-let-7i, hsa-mir-23b, hsa-mir-125b-1, hsa-mir-132, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-140, hsa-mir-141, hsa-mir-152, hsa-mir-191, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-149, hsa-mir-150, hsa-mir-320a, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-101-2, hsa-mir-99b, hsa-mir-26a-2, hsa-mir-379, hsa-mir-423, hsa-mir-451a, hsa-mir-486-1, hsa-mir-496, hsa-mir-520a, hsa-mir-525, hsa-mir-518b, hsa-mir-516b-2, hsa-mir-516b-1, hsa-mir-516a-1, hsa-mir-516a-2, hsa-mir-92b, hsa-mir-320b-1, hsa-mir-320c-1, hsa-mir-320b-2, bta-mir-26a-2, bta-let-7f-2, bta-mir-101-2, bta-mir-103-1, bta-mir-16b, bta-mir-20a, bta-mir-21, bta-mir-27a, bta-mir-125a, bta-mir-125b-1, bta-mir-199a-1, bta-mir-31, bta-mir-140, bta-mir-92a-2, bta-let-7d, bta-mir-132, bta-mir-191, bta-mir-192, bta-mir-22, bta-mir-23a, bta-mir-29c, bta-mir-423, bta-let-7g, bta-mir-24-2, bta-let-7a-1, bta-mir-150, bta-let-7f-1, bta-mir-30c, bta-let-7i, bta-mir-23b, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-125b-2, bta-mir-99b, hsa-mir-1249, hsa-mir-103b-1, hsa-mir-103b-2, hsa-mir-320d-1, hsa-mir-320c-2, hsa-mir-320d-2, bta-mir-101-1, bta-mir-133a-2, bta-mir-133a-1, bta-mir-141, bta-mir-152, bta-mir-16a, bta-mir-24-1, bta-mir-199a-2, bta-mir-223, bta-mir-26a-1, bta-mir-379, bta-mir-451, bta-mir-486, bta-mir-496, bta-mir-92a-1, bta-mir-92b, bta-mir-1249, bta-mir-320b, bta-mir-320a-1, hsa-mir-320e, hsa-mir-23c, hsa-mir-451b, bta-mir-149, hsa-mir-486-2
In sheep, miR-30c, miR-132, miR-379, miR-199a-3p and miR-320 are differentially expressed in serum on Days 30 or 60 of pregnancy [27]. [score:3]
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[+] score: 2
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-21, hsa-mir-22, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, hsa-mir-26a-1, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-99a, mmu-let-7g, mmu-let-7i, mmu-mir-27b, mmu-mir-99a, mmu-mir-140, mmu-mir-10b, mmu-mir-181a-2, mmu-mir-24-1, mmu-mir-191, hsa-mir-192, hsa-mir-148a, hsa-mir-30d, mmu-mir-122, hsa-mir-10b, hsa-mir-181a-2, hsa-mir-181a-1, mmu-let-7d, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-122, hsa-mir-140, hsa-mir-191, hsa-mir-320a, mmu-mir-30d, mmu-mir-148a, mmu-mir-192, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-21a, mmu-mir-22, mmu-mir-24-2, mmu-mir-26a-1, mmu-mir-92a-2, mmu-mir-25, mmu-mir-181a-1, mmu-mir-26a-2, mmu-mir-92a-1, hsa-mir-26a-2, hsa-mir-423, hsa-mir-451a, mmu-mir-451a, hsa-mir-486-1, mmu-mir-486a, mmu-mir-423, bta-mir-26a-2, bta-let-7f-2, bta-mir-148a, bta-mir-21, bta-mir-30d, bta-mir-99a, bta-mir-181a-2, bta-mir-27b, bta-mir-140, bta-mir-92a-2, bta-let-7d, bta-mir-191, bta-mir-192, bta-mir-22, bta-mir-423, bta-let-7g, bta-mir-10b, bta-mir-24-2, bta-let-7a-1, bta-let-7f-1, bta-mir-122, bta-let-7i, bta-mir-25, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, hsa-mir-1246, bta-mir-24-1, bta-mir-26a-1, bta-mir-451, bta-mir-486, bta-mir-92a-1, bta-mir-181a-1, bta-mir-320a-1, mmu-mir-486b, hsa-mir-451b, bta-mir-1246, mmu-mir-21b, mmu-let-7j, mmu-mir-21c, mmu-mir-451b, mmu-let-7k, hsa-mir-486-2
Bta-miR-320a and bta-miR-192 had the greatest number of copies during fall, 2013, while spring, 2014, had the fewest (P< 0.02). [score:1]
There were three microRNAs (bta-miR-1246, bta-miR-21-5p, and bta-miR-320a) that were identified in the present study, but unreported by. [score:1]
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[+] score: 1
We have identified 13 highly abundant miRNAs with Ct numbers between 10–25 Ct (bta-miR-615, bta-miR-27a-5p, bta-miR-631, bta-miR-323, bta-miR-574, bta-miR-767, bta-miR-320a, bta-miR-411b, bta-miR-421, bta-miR-99b, bta-miR-382, bta-miR-669 and bta-miR-760-5p) (Table 3). [score:1]
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[+] score: 1
Other miRNAs from this paper: bta-mir-29a, bta-let-7f-2, bta-mir-103-1, bta-mir-151, bta-mir-30d, bta-mir-126, bta-mir-181a-2, bta-mir-27b, bta-mir-30b, bta-mir-31, bta-mir-34b, bta-mir-107, bta-mir-15b, bta-mir-181b-2, bta-mir-30e, bta-let-7d, bta-mir-124a-1, bta-mir-138-2, bta-mir-181c, bta-mir-214, bta-mir-455, bta-mir-93, bta-let-7g, bta-mir-10b, bta-mir-30a, bta-let-7a-1, bta-mir-487b, bta-let-7f-1, bta-mir-122, bta-mir-30c, bta-let-7i, bta-mir-25, bta-mir-34c, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-15a, bta-mir-34a, bta-mir-1-2, bta-mir-1-1, bta-mir-105b, bta-mir-124a-2, bta-mir-124b, bta-mir-133a-2, bta-mir-133a-1, bta-mir-133b, bta-mir-138-1, bta-mir-152, bta-mir-181d, bta-mir-196a-2, bta-mir-196a-1, bta-mir-206, bta-mir-30f, bta-mir-409a, bta-mir-432, bta-mir-486, bta-mir-495, bta-mir-543, bta-mir-9-1, bta-mir-9-2, bta-mir-1185, bta-mir-1271, bta-mir-181a-1, bta-mir-181b-1, bta-mir-2284i, bta-mir-2284s, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-2284p, bta-mir-2284u, bta-mir-2363-1, bta-mir-2363-2, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2384, bta-mir-2284v, bta-mir-2284q, bta-mir-2404-1, bta-mir-2284m, bta-mir-2284b, bta-mir-320b, bta-mir-2424, bta-mir-2284r, bta-mir-2284h, bta-mir-2404-2, bta-mir-2284o, bta-mir-2284e, bta-mir-320a-1, bta-mir-424, bta-mir-2284w, bta-mir-2284x, bta-mir-409b, bta-mir-2284y-1, bta-mir-2284y-2, bta-mir-2284y-3, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2284y-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2284z-4, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-2284aa-4, bta-mir-2284z-2, bta-mir-133c, bta-mir-2284ab, bta-mir-2284ac
In contrast, five conserved miRNAs (bta-miRNA-543, miRNA-432, let-7i, miRNA-320, and miRNA-152) and seven novel miRNAs (bta-miRn16, miRn18, miRn33, miRn65, miRn70, miRn73, and miRn73) could be quantified from all tissues and several of them (e. g., bta-miRNA-320, miRn33 and miRn65) were relatively consistent across all nine tissue types. [score:1]
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