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7 publications mentioning bmo-mir-263a

Open access articles that are associated with the species Bombyx mori and mention the gene name mir-263a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 31
The most abundantly expressed miRNAs in B. mori are also highly conserved miRNAs e. g, miR-1, miR-8, miR-10, let-7, miR-263a, miR276a, and miR-306 and were expressed in all four stages, albeit their expression levels vary across different developmental periods. [score:8]
miR-306 is fairly uniformly expressed across the developmental stages, as judged by the number of reads, whereas miR-263 is dynamically regulated from the larva to adult stages. [score:5]
Additionally, we detected two antisense miRNA loci (miR-263-S and miR-263-AS; miR-306-S and miR-306-AS) that are expressed in sense and antisense directions. [score:4]
Interestingly, miR-263 and miR-306 are preferentially and abundantly expressed in pupae and adults, respectively. [score:3]
Comparing the mature sequences in each sense and antisense pair showed the seed region in miR-263 and miR263-AS to be almost identical, whereas miR-306 and miR-306-AS showed variations in several internal positions, including the seed region (Figure 6e). [score:1]
Predicted fold-back of miR-306 (a) and miR-263 (b) from sense (top panel) and antisense strand (bottom panel) and mature miRNA is shown in red. [score:1]
The top three abundant miRNAs of the silkworm are miR-1 (30%), miR-8 (11%) and miR-306 (11%) in the feeding larval stage; miR-1 (40%), miR-8 (18%), and miR-276a (15%) in the spinning larval stage; miR-276a (27%), miR-1 (16%) and miR278 (8%) in the pupal stage; miR-263a (18%), miR276a (16%) and miR-1 (12%) in the adult stage (Additional file 2). [score:1]
Mature sequences of miR-306:miR-306-AS pair (e) and miR-263:miR263-AS (f) aligned for showing identical nucleotide sequences. [score:1]
It includes miR-1, the entire family of miR-2, the miR-9 family (miR-9 and miR-9b), the let-7 family (let-7a, let-7j), miR-10b, miR-31, miR-71, miR-79, miR-87, miR-98, miR-100, miR-252, miR-263a, miR-275, miR-279, miR-317 and miR-1274b (Table 3 and Figure 4a). [score:1]
We recovered eight sequence reads that uniquely mapped to the antisense hairpin sequence of miR-306 (miR-306-AS) and a single read mapping to the antisense hairpin of miR-263 (miR-263-AS) (Figure 6c and 6d), which indicates that the antisense transcripts were processed into mature miRNAs in vivo. [score:1]
Our sequencing efforts identified, for the first time, the prevalence of at least two new miRNA loci, miR-306 and miR-263, that generate miRNAs by convergent transcription from both strands in the silkworm genome. [score:1]
miR-306 and miR-263 loci could generate antisense miRNAs in silkworm. [score:1]
Identification of three antisense miRNAs for miR-306, miR-263, and miR-iab-4-AS in silkworm suggests that such antisense miRNAs may be more widespread in other organisms. [score:1]
Sequencing reads uniquely aligned to hairpin sequence of reverse complement of miR-306 (c) and miR-263 (d) followed by their cloning frequency and length (nt). [score:1]
Analysis of the antisense sequences of miR-306-AS and miR-263-AS loci by mFold showed that they can adopt canonical hairpin secondary structures (Figure 6a and 6b). [score:1]
[1 to 20 of 15 sentences]
2
[+] score: 15
For instance, aside from a few commonly expressed miRNAs, such as bmo-miR-8, bmo-miR-13, bmo-miR-263a, bmo-miR-275, bmo-miR-279, bmo-282*, bmo-miR-285 and bmo-miR-306, most of them were found development-related (Table 3) although some may be due to inadequate sampling that often results less reliable statistics. [score:4]
Especially in bmo-miR-263a (Figure 3), changes of one nucleotide in 5′ or more in 3′ ends may not affect their regulatory roles because a mechanism for selecting target genes is based on nucleotide shuffling of a 7-nt seed sequence starting from the second nucleotide at the 5′ end of miRNAs. [score:4]
For analyzing the expression patterns of bmo-miR-1, bmo-miR-13, bmo-miR-14, bmo-miR-77, bmo-miR-263a, bmo-miR-275 and U6 snRNA, real-time PCR was performed in an ABI Prism® 7300 Sequence Detection system with Quant SYBR Green PCR kit (TIANGEN, BJ) following the manufacturer's instructions. [score:3]
Direct cloning allowed us to identify several mature miRNAs sequences, such as bmo-miR-263a, bmo-miR-282*, and bmo-miR-317, which vary in their 5′ and/or 3′ ends on the same stem of their precursors (Table 3). [score:2]
Bmo-miR-8, bmo-miR-9a, and bmo-miR-263a, similar to bmo-miR-1, showed a slight elevation after diapause-broken stage (DBS) and kept a relatively constant level thereafter. [score:1]
We also found several miRNA*s, such as bmo-miR-263a* in bluish egg (BS), bmo-miR-71* in spinning larva (SS) and pre-pupa (PPS), which providing solid evidence for Dicer-like processing [55], [56], [57], as it was reported that miRNA*s can also be functional [43], [58], [59]. [score:1]
[1 to 20 of 6 sentences]
3
[+] score: 6
Pre-miRNAs, structures, and multiple isoforms of expressed mature bmo-mir-263a, bmo-let-7a and bmo-mir-317 sequences and their read counts are shown. [score:3]
Seven families of conserved miRNAs, including four clusters described above (2, 4, 7, and 8), have paralogs in the silkworm genome (mir-2, mir-993, mir-9, mir-92, mir-263, mir-279, and mir-989) (Figure 4). [score:1]
The read counts of bmo-miR-263a were at least 1000-fold higher than those of bmo-miR-263b in each library. [score:1]
As exemplified by bmo-miR-263a, let-7a, and miR-317 in Figure 2, the highlighted isoform of mature sequences is more highly accumulated in the three libraries than is the previously annotated sequence, and should be regarded as the final functional molecule. [score:1]
[1 to 20 of 4 sentences]
4
[+] score: 4
bmo-let-7b, bmo-let-7c, bmo-miR-9, bmo-miR-9*, bmo-miR-100-like, bmo-miR-263a, bmo-miR-31 and bmo-bantam were expressed in larva and pupa, but were not detected in moth; of these miRNAs, bmo-miR-9 and bmo-miR-9* are also complementary miRNAs. [score:3]
These newly identified miRNAs are bmo-miR-2a*, bmo-miR-8*, bmo-miR-13a*, bmo-miR-46*, bmo-miR-263*, bmo-miR-279*, and bmo-miR-305*. [score:1]
[1 to 20 of 2 sentences]
5
[+] score: 3
Members of MIR-263, MIR-281 and MIR-9 were also highly expressed in the 4 samples. [score:3]
[1 to 20 of 1 sentences]
6
[+] score: 2
For instance, bmo-bantam, bmo-miR-12, bmo-miR-263a, bmo-miR-263b, bmo-miR-278, and bmo-miR-8 are both literature -based and database collections but the miRBase-collected miRNAs showed stronger signals. [score:1]
For instance, the number of reads for bmo-miR-263a is extremely high as compared to other miRNAs in all libraries; it may play a very important role in the posterior gland development and the result is in agreement with a previous report [20]. [score:1]
[1 to 20 of 2 sentences]
7
[+] score: 1
However, a higher number of miRNAs were initially present in day 9 embryos (9 d Em) at the body pigmentation stage, including miR-281-5p, miR-281-3p, miR-263, miR-970, miR-133, miR-283, and miR-317. [score:1]
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