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11 publications mentioning mmu-mir-654

Open access articles that are associated with the species Mus musculus and mention the gene name mir-654. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 98
Importantly, restoration of miR-654-3p upregulated the expression of the metastasis-suppressor genes BRMS1, MTSS1, KISS1 and NME1, and downregulated the expression of several genes related to tumor progression and metastasis, including MTA1, MTA2, SERPINE1, SSTR2 and CD44. [score:13]
Known tumor suppressor genes were upregulated after transfection of mimetic miR-654-3p, including CASP8, PTEN, DAPK1, RB1 and the cell cycle regulator CDKN2A. [score:7]
Importantly, the miRNAs miR-495-3p, miR-654-3p, miR-376a-3p and miR-487b-3p exhibited marked downregulation after 5 weeks in contrast to a slight reduction of expression observed for most miRNA genes from this region. [score:6]
miR-654-3p, which has been described as a tumor suppressor miRNA in prostate cancer cell lines [28], is downregulated during PTC progression in the Tg-Braf mo del and in human PTC samples. [score:6]
miR-654-3p is downregulated during tumor progression of PTC in vivo and regulates cell proliferation and migration of PTC in vitro. [score:5]
Figure 4 miR-654-3p is downregulated during tumor progression of PTC in vivo and regulates cell proliferation and migration of PTC in vitro a. Thyroid tissue extracted from 5-,12- and 30-weeks old animals (Tg-Braf and WT) was homogenized and total RNA extracted was used for cDNA synthesis. [score:5]
Among the differentially expressed miRNAs from the 14q32 region, we observed that miR-654-3p levels decrease with long-term PTC progression in Tg-Braf mice and inversely correlate with the expression of the EMT markers Zeb1, Zeb2, Snai1 and Snai2 (Figure 4a). [score:5]
Although no morphological changes were observed, transfection of miR-654-3p increased the expression of CDH1 and CTNNA1, and decreased the expression of SNAI2, which is consistent with an “epithelial” genetic program. [score:5]
Our functional analysis indicates that miR-654-3p markedly decreases cell proliferation and increases apoptosis, possibly by restoring the expression of key tumor suppressor genes. [score:5]
Moreover, key genes involved in cell adhesion, migration and ECM remo deling were downregulated after transfection of mimetic miR-654-3p, such as LAMB1 and MMP9. [score:4]
The analysis of randomly-chosen miRNAs from clusters 1 and 2 (miR-654-3p, mir-369-3p, miR-495, miR-370-5p, miR-127-5p and miR-376c-3p) in tumor cell lines confirmed their downregulation (Figure 1d). [score:4]
Furthermore, we show tumor suppressor properties for miR-654-3p in vitro, which could open perspectives for new therapeutic strategies for PTC. [score:3]
The restoration of miR-654-3p expression using commercial mimetic miRNA markedly decreased cell proliferation and migration, and increased apoptosis in normal and tumor thyroid cell lines (Figure 4b and 4c). [score:3]
The miR-654-3p -transfected cells showed increased expression levels of the epithelial cell markers CDH1 and CTNNA1 and decreased levels of SNAI2, with no significant changes in ZEB1, ZEB2, SNAI1 and VIM levels (Figure 4d). [score:3]
To evaluate the participation of miR-654-3p in PTC progression, we analyzed the impact of ectopic expression of miR-654-3p on the expression of several genes involved in tumor establishment and progression using a Taqman® Human Tumor Metastasis qPCR Array. [score:3]
Values in Y axis reflect the log [2] fold change expression levels between BCPAP cells transfected miR-654-3p mimetic with those not transfected. [score:3]
Finally, in vitro functional analyses highlight tumor suppressor properties for miR-654-3p in PTC. [score:3]
Tumor suppressor properties of miR-654-3p in thyroid cell lines. [score:3]
Importantly, the levels of key modulators of tumor progression, such as ECM and ECM-remo deling genes, as well as metastasis-suppressing and -promoting genes, were restored after transfection of a miR-654-3p mimetic. [score:3]
No morphological changes were observed through light microscopy after transfection of miR-654-3p (data not shown). [score:1]
b. N-Thy-ORI and TPC-1 cells were transfected or not with miR-654-3p mimetic and after 24, 48, and 72 h, cells were trypsinized and counted. [score:1]
c. TPC-1, BCPAP and KTC-2 cells transfected or not with miR-654-3p mimetic were seeded into the upper compartment of modified Boyden chambers with 8 μm pore inserts as described in Methods section. [score:1]
Altogether, these data suggest that restoration of miR-654-3p could contribute to a more differentiated, less aggressive phenotype in PTC. [score:1]
Normal and tumor thyroid cells were transfected with miR-654-3p miRVana mimic (Life Technologies) at concentration of 10-30nM. [score:1]
Also, decreased levels of HRAS, FGF2, FGFR4 and IL1B, as well as increased NF1, components of MAPK signal transduction, were observed after transfection of miR-654-3p. [score:1]
snRNA U6 and RPL19 were used for normalization of miR-654-3p and Zeb1 Zeb2, Snai1 and Snai2 respectively. [score:1]
d. BCPAP cells were transfected with 30nM miR-654-3p mimetic. [score:1]
Transient transfection of miR-654-3p mimetic. [score:1]
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2
[+] score: 28
These analyses identified four miRNAs, miR-146a (upregulated twofold), miR-221, miR-654-5p, and miR-656 (all three down-regulated ~1.3-fold), significantly deregulated in the patients (P < 0.05) (see Fig.   1a). [score:8]
miR-654-5p and miR-656 are conserved only in the primate chain and are expressed at low level in the human brain; by contrast, miR-146a and miR-221 sequences are 100 % conserved in the mouse and are highly expressed in different human brain regions throughout development including the cortex, hippocampus, and cerebellum (Allen Brain Atlas, Brain Span) [23], supporting a role in development. [score:7]
a Significantly deregulated miRNAs after validation in ASD OMSC after the validation round, representing the miRNA deregulation signature of ASD: miR-146a, miR-221, miR-654-5p, and miR-656 (±SD, controls— dark gray bar, patients— light gray bar). [score:3]
Expression (±SD) of miR-146a, miR-221, miR-654-5p, and miR-656 were assessed in primary skin fibroblasts of ASD patients (n = 5, light gray bar), ID patients (n = 12, white bar), and controls (n = 4, dark gray bar). [score:3]
Expression profiles of miR-146a, miR-221, miR-654-5p, and miR-656 were assessed using Taqman assays on Fluidigm 48.48 array in technical triplicates; for fibroblast samples, two different miRNA extractions from consecutive passages were analyzed and six miRNAs were used as reference miRNAs (see Additional file 1: Figure S2 and Additional file 2 for raw data); for peripheral blood mononuclear cells (PBMC), only one extraction was analyzed and seven miRNAs were used as reference miRNAs (see Additional file 1: Figure S3 and Additional file 2 for raw data). [score:2]
We identified a signature of four miRNAs (miR-146a, miR-221, miR-654-5p, and miR-656) commonly deregulated in ASD. [score:2]
We identified a signature of four miRNAs (miR-146a, miR-221, miR-654-5p, and miR- 656) commonly deregulated in ASD. [score:2]
This analysis showed a significant deregulation of miR-146a, miR-221, and miR-654-5p in the ASD group compared to controls in the same trend as in OMSC (see Fig.   1b). [score:1]
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3
[+] score: 6
Expression of the most upregulated miRNA, miR-654, changed ∼30-fold during differentiation. [score:6]
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4
[+] score: 5
Furthermore, AIV infection leads to the differential expression of cellular miRNA in chickens and mice, and miR491 and miR654 inhibit the replication of H1N1 virus through binding to PB1 in MDCK cells (Song et al., 2010). [score:5]
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5
[+] score: 4
As found for 10-87 HP cells and 10-87 T cells, SF-VERO cells and A4497 VERO cells expressed increased levels of miR-376a, miR-654-3p, miR-543, and miR-382 over the levels found in pAGMK cells (Table 4). [score:3]
qRT-PCR analysis confirmed that miR-376a, miR-654-3p, miR-543, miR-382, miR-31, miR-200c, miR-218, and miR-183 paralleled the microarray miRNA levels. [score:1]
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6
[+] score: 3
This miRNA is part of a large cluster, two of which (miR-654/376b) were found to be expressed strongly in murine embryonic cartilage [74]. [score:3]
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7
[+] score: 3
Moreover, several studies have demonstrated that cellular microRNAs (miR-323, miR-491, miR654, miR-146a) inhibit influenza virus replication or propagation [23, 24]. [score:3]
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8
[+] score: 3
Additionally, prior studies from various mo dels of retinal degeneration identified over 300 differentially expressed miRNAs 63– 90, a total of 16 common miRNAs were identified (miR-1187, miR-125b-5p, miR-331-3p, miR466d-3p, miR-467f, miR-542-3p, miR-574-5p, miR654-3p, miR669h-3p, miR-882, miR-342-3p, miR-466a-5p, miR-466d-5p, miR-706, miR-345-3p, miR532-5p). [score:3]
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9
[+] score: 2
Wei J. Q. Chen H. Zheng X. F. Zhang B. X. Wang Y. Tang P. F. She F. Song Q. Li T. S. Hsa-miR-654–5p regulates osteogenic differentiation of human bone marrow mesenchymal stem cells by repressing bone morphogenetic protein 2 J. Southern Med. [score:2]
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10
[+] score: 1
Formosa A. Markert E. K. Lena A. M. Italiano D. Finazzi-Agro E. Levine A. J. Bernardini S. Garabadgiu A. V. Melino G. Candi E. MicroRNAs, miR-154, miR-299-5p, miR-376a, miR-376c, miR-377, miR-381, miR-487b, miR-485-3p, miR-495 and miR-654-3p, mapped to the 14q32. [score:1]
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11
[+] score: 1
Additionally, seven miRNAs exhibited a consistent pattern of no amplification in TEC from infected animals (miR-144, miR-208b, miR-291b-3p, miR-295, miR-302a, miR-488, and miR-654-3p, Figure S4 in). [score:1]
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