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11 publications mentioning hsa-mir-892b

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-892b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 80
Therefore, in order to confirm the involvement of (1) hsa-miR-145 on the expression of its predicted target mRNA Cldn10 and of (2) hsa-miR-892b on Esrrg mRNA, we cloned the 3′UTR regions of these targets in a luciferase reporter system. [score:7]
We confirmed that miR-145 and miR-892b inhibit the expression of the luciferase reporter via Cldn10 and Esrrg 3′ UTRs, respectively. [score:5]
Immortalized human epididymal epithelial cells [47] were used for experimental confirmation of the role of miR-145 on the expression of Cldn10 and miR-892b on the expression of Esrrg. [score:5]
Correlation between the expression of Cldn10 and Esrrg with the expression of miR-145 and miR-892b. [score:5]
Esrrg expression was negatively correlated with the expression of both miR-892b and miR-891b. [score:5]
Interestingly, a strong negative correlation (r = −0,89, P-value≤0.001) was found between the pattern of expression of miR-892b and its potential mRNA target Esrrg (Estrogen Related Receptor Gamma) and with miR-145 and Cldn10 mRNA (r = −0,92, P-value≤0.001). [score:5]
Considering the role of Cldn10 in epididymal functions and the presumed importance of cluster miR-888 target genes such as Esrrg, we focused our interest on these two genes and conducted an experimental approach to determine if Cldn10 was regulated by miR-145 and Esrrg by miR-892b. [score:4]
Experimental confirmation of target genes regulation by miR-145 and miR-892b using a luciferase reporter system. [score:4]
0034996.g004 Figure 4 The expression of miR-890, miR-891a, miR-891b, miR-892a and miR-892b was assessed in total RNA extracts from the human caput, corpus and cauda epididymis from three donors. [score:3]
miR-205 followed the same pattern of expression as miR-890/miR-891a/miR-891b/miR-892a/miR-892b, with higher levels in the distal regions of the epididymis (Fig. 3 C). [score:3]
For instance, miR-892b and miR-891a were highly and significantly (p<0.01) more expressed in the cauda than in the caput epididymidis, with 170- and 102-fold changes, respectively (Fig. 2 A and C). [score:3]
Overall, these results show that the levels of miR-890/miR-891a/miR-891b/miR-892a/miR-892b and miR-205, previously reported to be primarily expressed in reproductive tissues, are present at high levels in the distal region of the epididymis, suggesting a role of these microRNAs in the later stages of epididymal sperm maturation or storage. [score:3]
24 hours later, co-transfection of 0.4 ug of plasmid DNA clones (control plasmid without miRNA target sequences, P-miR-Cldn10 or P-miR-Esrrg) with synthetic miRNAs that mimic endogenous miRNAs (5 nM Syn-hsa-miR-145 miScript miRNA Mimic or 5 nM Syn-hsa-miR-892b miScript miRNA Mimic (Qiagen, Toronto, ON, Canada)) was performed by using the Attractene transfection reagent (Qiagen) according to the manufacturer's protocol. [score:3]
The expression of miR-890, miR-891a, miR-891b, miR-892a and miR-892b was assessed in total RNA extracts from the human caput, corpus and cauda epididymis from three donors. [score:3]
In order to confirm the role of miR-145 on the expression of Cldn10 and miR-892b on Esrrg, we conducted a series of co-transfection experiments by using mimic Syn-hsa-miR-145 and Syn-hsa-miR-892b along with a luciferase reporter system containing the 3′UTR sequence of Cldn10 or Esrrg (Fig. 7). [score:3]
Among these miRNAs, miR-890, miR-892a, miR-892b, miR-891a, miR-891b belonging to the same epididymis-enriched cluster located on the X chromosome, are significantly more expressed in the corpus and cauda regions than in the caput. [score:3]
These data suggest that hsa-miR-892b significantly and specifically affects the expression of Esrrg at the post-transcriptional level. [score:3]
Whereas we demonstrated that Esrrg expression is regulated at the post-transcriptional level by the epididymis-specific miR-892b, the role and significance of Esrrg in the epididymis has to be investigated. [score:2]
We therefore confirmed the respective roles of miR-145 and miR-892b, in the post-transcriptional regulation of Cldn10 and Esrrg. [score:2]
Five of six miRNAs located in the miR-888 cluster displayed changes ranging from 1.7-fold for miR-891b to 126-fold for miR-892b, between the caput and the corpus region (miR-890/miR-891a/miR-891b/miR-892a/miR-892b). [score:1]
A control plasmid (P-miR), or plasmids containing the 3′UTR sequences of Cldn10 (P-miR-Cldn10) or Esrrg (P-miR-Esrrg) were co -transfected in the presence (+) or in the absence (−) of mimics Syn-Hsa-miR-145 or Syn-Hsa-miR-892b. [score:1]
Our results indicate that members of the miR-888 cluster (i. e. miR-890/miR-891a/miR-891b/miR-892a/miR-892b) and miR-205 exhibit significantly lower levels in the caput relative to the corpus, while the levels of the miR-371 cluster did not differ (Fig. 3 A, B and C). [score:1]
Both end-point and relative quantification by real time PCR of miR-890/miR-891a/miR-891b/miR-892a/miR-892b confirmed the microarray data (Fig. 4). [score:1]
Very low signals for miR-890/miR-891a/miR-891b/miR-892a/miR-892b were observed by PCR in the caput, whereas strong signals were detected in the corpus and cauda. [score:1]
0034996.g007 Figure 7 A control plasmid (P-miR), or plasmids containing the 3′UTR sequences of Cldn10 (P-miR-Cldn10) or Esrrg (P-miR-Esrrg) were co -transfected in the presence (+) or in the absence (−) of mimics Syn-Hsa-miR-145 or Syn-Hsa-miR-892b. [score:1]
In addition, the gene encoding for estrogen-related receptor gamma (Esrrg) was negatively correlated with two members of the miR-888 cluster, miR-892b (Tables 2 and 3, Fig. 6, Fig. S3) and miR-891b (Tables 2 and 3, Fig. S3). [score:1]
Co-transfection of P-miR-Esrrg with hsa-miR-892b resulted in a significant decrease in luciferase activity relative to P-miR-Esrrg transfected alone. [score:1]
Luciferase activity was not affected by the presence of miR-892b which does not have any binding sites on the 3′UTR of Cldn10 (Fig. 7, P-miR-Cldn10). [score:1]
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2
[+] score: 9
7 miRNAs (hsa-miR-144,hsa-miR-133a,hsa-miR-365, hsa-miR-424,hsa-miR-500, hsa-miR-661,hsa-miR-892b) had different gene expression levels between active TB and healthy controls; 4 of them (hsa-miR-144,hsa-miR-365 and hsa-miR-133a, hsa-miR-424) were up-regulated and 3 of them (hsa-miR-500, hsa-miR-661,hsa-miR-892b) were down-regulated in active TB patients. [score:9]
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3
[+] score: 8
From the 13 cfs-miRNAs previously identified to be predominately derived from the epididymis [21], five miRNAs (miR-891b, miR-892b, miR-892a, miR-888 and miR-890), were significantly down-regulated (P=0.000, 0.001, 0.025, 0.001, 0.000, respectively) in men with UA, as compared with the normozoospermic control donors (Figure 1). [score:3]
The results suggested that miR-891b, miR-892b, miR-892a, miR-888 and miR-890 were correlated with sperm progressive motility separately (r=0.287, 0.395, 0.279, 0.346 and 0.301, p=0.001, 0.000, 0.001, 0.000 and 0.001 respectively) (A-E). [score:1]
Levels of miR-891b/miR-892b/miR-892a/miR-888/miR-890 combination (miRNA panel) was also significantly correlated with sperm progressive motility (r=0.218, p=0.000) (Figure 3F). [score:1]
After identifying 5 members of miR-888 cluster (miR-891b, miR-892b, miR-892a, miR-888 and miR-890) dysregulated in UA patients, we analyzed characteristics of these 5 miRNAs dysregulation in validation set. [score:1]
Levels of miR-891b/miR-892b/miR-892a/ miR-888/miR-890 combination (miRNA panel) was also significantly correlated with sperm progressive motility (r=0.218, p=0.000) (F). [score:1]
We found that miRNA level of miR-891b, miR-892b, miR-892a, miR-888 and miR-890 were correlated with sperm progressive motility separately (r=0.287, 0.395, 0.279, 0.346 and 0.301, p=0.001, 0.000, 0.001, 0.000 and 0.001 respectively) (Figure 3A-3E). [score:1]
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[+] score: 5
Five miRs have one single target gene (miR-202: CBFA2T3, miR-500: NEBL, miR-378: KIF26A, miR-892b: BACH2, miR-1305:ID1), with a restricted impact on gene expression modulation. [score:5]
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[+] score: 3
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-17, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, hsa-mir-29a, hsa-mir-30a, hsa-mir-31, hsa-mir-32, hsa-mir-33a, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-106a, mmu-let-7g, mmu-let-7i, mmu-mir-27b, mmu-mir-30a, mmu-mir-30b, mmu-mir-126a, mmu-mir-9-2, mmu-mir-135a-1, mmu-mir-137, mmu-mir-140, mmu-mir-150, mmu-mir-155, mmu-mir-24-1, mmu-mir-193a, mmu-mir-194-1, mmu-mir-204, mmu-mir-205, hsa-mir-30c-2, hsa-mir-30d, mmu-mir-143, mmu-mir-30e, hsa-mir-34a, hsa-mir-204, hsa-mir-205, hsa-mir-222, mmu-let-7d, mmu-mir-106a, mmu-mir-106b, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-30b, hsa-mir-135a-1, hsa-mir-135a-2, hsa-mir-137, hsa-mir-140, hsa-mir-143, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-126, hsa-mir-150, hsa-mir-193a, hsa-mir-194-1, mmu-mir-19b-2, mmu-mir-30c-1, mmu-mir-30c-2, mmu-mir-30d, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-15a, mmu-mir-23a, mmu-mir-24-2, mmu-mir-29a, mmu-mir-31, mmu-mir-92a-2, mmu-mir-34a, rno-mir-322-1, mmu-mir-322, rno-let-7d, rno-mir-329, mmu-mir-329, rno-mir-140, rno-mir-350-1, mmu-mir-350, hsa-mir-200c, hsa-mir-155, mmu-mir-17, mmu-mir-25, mmu-mir-32, mmu-mir-200c, mmu-mir-33, mmu-mir-222, mmu-mir-135a-2, mmu-mir-19b-1, mmu-mir-92a-1, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-7b, hsa-mir-194-2, mmu-mir-194-2, hsa-mir-106b, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-30e, hsa-mir-375, mmu-mir-375, mmu-mir-133b, hsa-mir-133b, rno-let-7a-1, rno-let-7a-2, rno-let-7b, rno-let-7c-1, rno-let-7c-2, rno-let-7e, rno-let-7f-1, rno-let-7f-2, rno-let-7i, rno-mir-7b, rno-mir-9a-1, rno-mir-9a-3, rno-mir-9a-2, rno-mir-17-1, rno-mir-19b-1, rno-mir-19b-2, rno-mir-23a, rno-mir-24-1, rno-mir-24-2, rno-mir-25, rno-mir-27b, rno-mir-29a, rno-mir-30c-1, rno-mir-30e, rno-mir-30b, rno-mir-30d, rno-mir-30a, rno-mir-30c-2, rno-mir-31a, rno-mir-32, rno-mir-33, rno-mir-34a, rno-mir-92a-1, rno-mir-92a-2, rno-mir-106b, rno-mir-126a, rno-mir-135a, rno-mir-137, rno-mir-143, rno-mir-150, rno-mir-193a, rno-mir-194-1, rno-mir-194-2, rno-mir-200c, rno-mir-200a, rno-mir-204, rno-mir-205, rno-mir-222, hsa-mir-196b, mmu-mir-196b, rno-mir-196b-1, mmu-mir-410, hsa-mir-329-1, hsa-mir-329-2, mmu-mir-470, hsa-mir-410, hsa-mir-486-1, hsa-mir-499a, rno-mir-133b, mmu-mir-486a, hsa-mir-33b, rno-mir-499, mmu-mir-499, mmu-mir-467d, hsa-mir-891a, hsa-mir-892a, hsa-mir-890, hsa-mir-891b, hsa-mir-888, rno-mir-17-2, rno-mir-375, rno-mir-410, mmu-mir-486b, rno-mir-31b, rno-mir-9b-3, rno-mir-9b-1, rno-mir-126b, rno-mir-9b-2, hsa-mir-499b, mmu-let-7j, mmu-mir-30f, mmu-let-7k, hsa-mir-486-2, mmu-mir-126b, rno-mir-155, rno-let-7g, rno-mir-15a, rno-mir-196b-2, rno-mir-322-2, rno-mir-350-2, rno-mir-486, mmu-mir-9b-2, mmu-mir-9b-1, mmu-mir-9b-3
Conversely, the 5 members of the miR-888 cluster (miR-890, miR-891a, miR-891b, miR-892a, and miR-892b) that have been reported as being highly expressed in the corpus and caudal regions of the human epididymis [12] were not detected in our analysis of the mouse epididymis or in previous work on the rat epididymis [7]. [score:3]
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[+] score: 3
Other miRNAs from this paper: hsa-let-7f-1, hsa-let-7f-2, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, hsa-mir-32, mmu-mir-1a-1, mmu-mir-133a-1, mmu-mir-134, mmu-mir-135a-1, mmu-mir-144, mmu-mir-181a-2, mmu-mir-24-1, mmu-mir-200b, mmu-mir-206, hsa-mir-208a, mmu-mir-122, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-181a-1, hsa-mir-214, hsa-mir-200b, mmu-mir-299a, mmu-mir-302a, hsa-mir-1-2, hsa-mir-122, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-135a-1, hsa-mir-135a-2, hsa-mir-144, hsa-mir-134, hsa-mir-206, mmu-mir-200a, mmu-mir-208a, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-24-2, mmu-mir-328, hsa-mir-200c, hsa-mir-1-1, mmu-mir-1a-2, hsa-mir-181b-2, mmu-mir-25, mmu-mir-32, mmu-mir-200c, mmu-mir-181a-1, mmu-mir-214, mmu-mir-135a-2, mmu-mir-181b-1, mmu-mir-181c, hsa-mir-200a, hsa-mir-302a, hsa-mir-299, hsa-mir-361, mmu-mir-361, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-367, hsa-mir-377, mmu-mir-377, hsa-mir-328, mmu-mir-133a-2, mmu-mir-133b, hsa-mir-133b, mmu-mir-181b-2, hsa-mir-20b, hsa-mir-429, mmu-mir-429, hsa-mir-483, hsa-mir-486-1, hsa-mir-181d, mmu-mir-483, mmu-mir-486a, mmu-mir-367, mmu-mir-20b, hsa-mir-568, hsa-mir-656, mmu-mir-302b, mmu-mir-302c, mmu-mir-302d, mmu-mir-744, mmu-mir-181d, mmu-mir-568, hsa-mir-892a, mmu-mir-208b, hsa-mir-744, hsa-mir-208b, mmu-mir-1b, hsa-mir-302e, hsa-mir-302f, hsa-mir-1307, eca-mir-208a, eca-mir-208b, eca-mir-200a, eca-mir-200b, eca-mir-302a, eca-mir-302b, eca-mir-302c, eca-mir-302d, eca-mir-367, eca-mir-429, eca-mir-328, eca-mir-214, eca-mir-200c, eca-mir-24-1, eca-mir-1-1, eca-mir-122, eca-mir-133a, eca-mir-144, eca-mir-25, eca-mir-135a, eca-mir-568, eca-mir-133b, eca-mir-206-2, eca-mir-1-2, eca-let-7f, eca-mir-24-2, eca-mir-134, eca-mir-299, eca-mir-377, eca-mir-656, eca-mir-181a, eca-mir-181b, eca-mir-32, eca-mir-486, eca-mir-181a-2, eca-mir-20b, eca-mir-361, mmu-mir-486b, mmu-mir-299b, hsa-mir-892c, hsa-mir-486-2, eca-mir-9021, eca-mir-1307, eca-mir-744, eca-mir-483, eca-mir-1379, eca-mir-7177b, eca-mir-8908j
More precisely, we identified five miRNAs expressed at the level > 10 cpm solely in PSSM GM muscle: eca-miR-144, eca-miR-20b, ecaub_novel-miR-472, ecaub_novel-miR-568, and ecaub_novel-miR-892. [score:3]
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[+] score: 1
Among them, miR-890, miR-888, miR-892a, and miR-892b are tightly clustered within a genomic region of 3 kb and are highly similar with each other, suggesting evolution of these members as a result of tandem duplication events. [score:1]
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8
[+] score: 1
Other miRNAs from this paper: hsa-mir-17, hsa-mir-28, hsa-mir-223, hsa-mir-127, hsa-mir-188, hsa-mir-194-1, hsa-mir-155, hsa-mir-194-2, hsa-mir-30e, hsa-mir-362, hsa-mir-363, hsa-mir-367, hsa-mir-379, hsa-mir-196b, hsa-mir-450a-1, hsa-mir-431, ssc-mir-28, hsa-mir-493, hsa-mir-512-1, hsa-mir-512-2, hsa-mir-500a, hsa-mir-501, hsa-mir-502, hsa-mir-450a-2, hsa-mir-513a-1, hsa-mir-513a-2, hsa-mir-506, hsa-mir-508, hsa-mir-509-1, hsa-mir-532, hsa-mir-615, hsa-mir-660, bta-mir-127, bta-mir-30e, bta-mir-17, bta-mir-450a-2, bta-mir-532, bta-mir-363, bta-mir-660, hsa-mir-891a, hsa-mir-892a, hsa-mir-509-2, hsa-mir-450b, hsa-mir-708, hsa-mir-509-3, hsa-mir-1285-1, hsa-mir-1285-2, hsa-mir-1248, ssc-mir-17, bta-mir-155, bta-mir-188, bta-mir-194-2, bta-mir-196b, bta-mir-223, bta-mir-28, bta-mir-362, bta-mir-367, bta-mir-379, bta-mir-431, bta-mir-493, bta-mir-500, bta-mir-502a-1, bta-mir-502a-2, bta-mir-502b, bta-mir-615, bta-mir-708, bta-mir-1248-1, bta-mir-1248-2, ssc-mir-450a, bta-mir-2320, bta-mir-1388, bta-mir-194-1, bta-mir-450a-1, eca-mir-30e, eca-mir-367, eca-mir-684, eca-mir-196b, eca-mir-615, eca-mir-708, eca-mir-194-1, eca-mir-493a, eca-mir-17, eca-mir-1248, eca-mir-28, eca-mir-127, eca-mir-379, eca-mir-431, eca-mir-493b, eca-mir-155, eca-mir-194-2, eca-mir-188, eca-mir-223, eca-mir-362, eca-mir-363, eca-mir-450a, eca-mir-450b, eca-mir-450c, eca-mir-500-1, eca-mir-500-2, eca-mir-501, eca-mir-502, eca-mir-508, eca-mir-509a, eca-mir-532, eca-mir-660, ssc-mir-30e, ssc-mir-196b-1, ssc-mir-450b, ssc-mir-127, ssc-mir-532, ssc-mir-708, ssc-mir-1285, ssc-mir-500, hsa-mir-514b, ssc-mir-363-1, ssc-mir-450c, hsa-mir-500b, ssc-mir-194b, ssc-mir-155, ssc-mir-362, bta-mir-3601, ssc-mir-615, ssc-mir-2320, bta-mir-450b, ssc-mir-194a, ssc-mir-196b-2, ssc-mir-363-2, ssc-mir-493, hsa-mir-892c, eca-mir-1388, eca-mir-514b, eca-mir-506a, eca-mir-509b, bta-mir-194b, ssc-mir-1388, ssc-mir-223, ssc-mir-660, bta-mir-194b-2, bta-mir-1949
However, the six miRNAs in the mir-892 cluster on chrX in human, are conserved in a cluster on chrX in pig (See Additional file 1: Figure S20). [score:1]
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[+] score: 1
These two FSHD1 fetal muscle biopsies share 11 miRNAs with similar modulations (Fig. 4), miR-1225–3p, miR-19b-1*, miR-208b, miR-22, miR-372, miR-383, miR-767–3p, miR-802, miR-872, miR-875–5p, and miR-892b. [score:1]
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[+] score: 1
circRNA-miRNA-mRNA/gene network showed that hsa-miR-138-5p and hsa-miR-30c-1-3p exhibited the most complicated interactions (Figure 4B), followed by hsa-miR-892b, hsa-miR-571, and hsa-miR-328-3p. [score:1]
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[+] score: 1
Analysis of the molecular interaction between hsa_circ_0004277 and numerous miRs was also predicted, identifying miR-138-5p, miR-30c-1-3p hsa-miR-892b, hsa-miR-571, and hsa-miR-328-3p as potential candidates [109]. [score:1]
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