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7 publications mentioning mmu-mir-873a

Open access articles that are associated with the species Mus musculus and mention the gene name mir-873a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

[+] score: 137
Other miRNAs from this paper: mmu-mir-211, mmu-mir-873b
Inhibition of miR-873 expression resulted in upregulation of CSMD3 in several hepatoma cell lines, indicating that CSMD3 is indeed a target of miR-873. [score:10]
The results showed that only upregulation of miR-873 and downregulation of its putative target genes, CSMD3 (CUB and Sushi multiple domains 3) and ATP8B2 (ATPase, aminophospholipid transporter, class I, type 8b, member 2), were consistently verified. [score:9]
To inhibit miR-873 expression in human and murine hepatoma cell lines, miR-873 inhibition plasmid was purchased. [score:7]
On the other hand, when miR-873 was overexpressed, suppression of CSMD3 expression was observed in Mahlavu, BNL and Hep-Y2 cells. [score:7]
Interestingly, following miR-873 upregulation, GRP78 expression was enhanced in most of the hepatoma cell lines. [score:6]
After verification process, only miR-873 -mediated downregulation of CSMD3 expression was consistently confirmed in an independent cohort of TgSW172* mice. [score:6]
These results indicated that pre-S/S-sW172* could upregulate miR-873 expression in at least two hepatoma cell lines (Hepa1-6 and HepY2). [score:6]
To understand whether the increased ER stress in transgenic mice was caused by pre-S/S-sW172* alone or also by miR-873 upregulation, the GRP78 levels were also compared between hepatoma cells with or without miR-873 overexpression. [score:5]
The plasmid was generated based on a lentivirus expression vector, TOOLSilent shRNA Vector (TOOLS Biotechnology, New Taipei City, Taiwan), with insertion of a short stretch DNA sequence complementary to a target area of the miR-873 stem-loop sequence. [score:5]
Notably, in almost all cell lines (except for Huh7 and HepY2), miR-873 activation led to enhanced cell proliferation, consistent with the view that its target, CSMD3, was a tumor suppressor gene. [score:5]
Finally, inhibition of miR-873 expression was achieved using a lentivirus -based vector in various hepatoma cell lines. [score:5]
Activation of miR-873 to target CSMD3 by pre-S/S-sW172* expression in hepatoma cell linesTo further clarify the regulation cascade, pTg-sW172* was transfected into BNL, Hepa1-6, HepY2, Huh7, J7, and Mahlavu cells and miR-873 was assayed 3 days after transfection. [score:5]
Following miR-873 inhibition, the CSMD3 expression remained undetectable in these three cell lines. [score:5]
These results were consistent with the actual changes of CSMD3 protein levels upon miR-873 inhibition (Figure 3f) and overexpression (Figure 4a). [score:5]
Activation of miR-873 to target CSMD3 by pre-S/S-sW172* expression in hepatoma cell lines. [score:5]
Inhibition of microRNA-873 expression. [score:5]
Besides increased ER stress response and apoptosis, resulting in enhanced hepatocyte proliferation and turnover, a miR-873 -mediated downregulation of CSMD3 contributed partly to hepatocarcinogenesis. [score:4]
Up-regulation of miR-873 was found in Hepa1-6 and HepY2 cells (Figure 4b). [score:4]
When examining the regulatory cascade from pre-S/S-sW172* to CSMD3 suppression, it was found that activation of miR-873 only occurred in Hepa1-6 and HepY2 cells, suggesting that other cellular factors were involved in this process. [score:4]
Increasing levels of miR-873 upregulation were noted (Figure 4c). [score:4]
The efficiency of anti-miR-873 expression was also assessed for comparison (Figure 3g). [score:3]
It was found that overexpression of miR-873 led to enhanced cell proliferation in Mahlavu, J7, Hepa1-6 and BNL cells. [score:3]
CSMD3 was undetectable in J7 cells with or without miR-873 overexpression. [score:3]
To understand whether CSMD3 was an authentic target of miR-873, the 3′-UTR of CSMD3 gene was inserted into the 3′-UTR of a luciferase gene for miR-873 co-transfection experiments. [score:3]
The targeted sequence of miR-873 was 5'-AGGAGACTCACAAGTTCCTG C-3'. [score:3]
Subsequently, miR-873 could target at CSMD3 by binding to the 3′-UTR of its transcripts. [score:3]
The putative miR-873-5p recognition seed site in the CSMD 3'UTR were subjected to site-specific mutagenesis by QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA) (CSMD 3'UTR mut), and the mutated sequences were validated via automated DNA sequencing. [score:2]
For measuring the miRNA expression level from cells transfected with antisense miR-873-5p or miR-873 mimics, the ΔCt value was used to calculate expression levels normalized against U6 RNA. [score:1]
To examine the effects of miR-873 on CSMD3 3'UTR, the pMIR-CSMD3-3'UTR (wt or mut) was co -transfected with pre-miR-873 or mock control vector into hepatoma cells. [score:1]
As such, the increased ER stress in transgenic mice not only caused by pre-S/S-sW172* accumulation but also by miR-873 activation. [score:1]
Only microRNA-211 and micriRNA-873 (miR-873) satisfied these criteria. [score:1]
To further clarify the regulation cascade, pTg-sW172* was transfected into BNL, Hepa1-6, HepY2, Huh7, J7, and Mahlavu cells and miR-873 was assayed 3 days after transfection. [score:1]
[1 to 20 of 32 sentences]
[+] score: 6
Only five miRNAs (mmu-miR-451, mmu-miR-223, mmu-miR-92a, mmu-miR-200c, and mmu-miR-873) were differentially expressed, implying that the majority of miRNA downregulation associated with obesity could be reversed by LFD treatment. [score:6]
[1 to 20 of 1 sentences]
[+] score: 4
In the order of the significance score by SAM, 15 up-regulated miRNAs are mmu-miR-127, mmu-miR-410, mmu-miR-433, mmu-miR-138, mmu-miR-181c, mmu-miR-382, mmu-miR-19b, mmu-miR-381, mmu-miR-666-3p, mmu-miR-376a, mmu-miR-873, mmu-miR-181a, mmu-miR-383, mmu-miR-181b, and mmu-miR-99b. [score:4]
[1 to 20 of 1 sentences]
[+] score: 4
Two miRNAs, miR-10b, and miR-873, were significantly downregulated in EtOHc-4D and AW8 neurons (data not shown). [score:4]
[1 to 20 of 1 sentences]
[+] score: 3
01 Stress response, cell proliferation miR-542 ↓2.60 NA miR-574 ↑2.09 Inflammation (Tlr9 activation), cell proliferation, apoptosis miR-669j ↑3.12 NA miR-672 ↑2.40 NA miR-674 ↑2.19 NA miR-744 ↑4.35Oncogene (Tgf) suppression miR-873 ↑2.53 ↑3.22 NA miR-1930 ↑3.31 NA miR-1934 ↑2.17 ↑3.27 NA miR-1942 ↑2.49 NA miR-3064 ↑2.50 NA miR-3065 ↑3.20 NA miR-3069 ↑2.98 NA miR-3071 ↑3.51 NA miR-3073 ↓2.78 NA miR-3092 ↑3.48 NA miR-3093 ↑3.28 NA miR-3109 ↓2.07 NAAll reported variations were statistically significant (P < 0.05). [score:3]
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[+] score: 3
miRNAs for Pik3cg (miR-707), Hbp1 (miR-127, miR-183, and miR-873), Twistnb (miR-718, and miR-691), and Dgkb (miR-489) had no impact on luciferase expression (Figure 2A and data not shown). [score:3]
[1 to 20 of 1 sentences]
[+] score: 1
86 miR-873-as 1,016 e-miR-743b-5p 23.75 miR-449c-as 515e-miR-715 || 69.35 miR-541-as 439 e-miR-881* 56.21 miR-148b-as 336 e-miR-370 97.41 miR-546-as 333 e-miR-3067 100 miR-3074-as 262 e-miR-448-5p 100miR-451-as ‡ 286 e-miR-669o-5p 99.35 † Novel miR* that are processed within the expected window of the mature strand are labelled “generic”. [score:1]
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