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10 publications mentioning hsa-mir-935

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-935. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

[+] score: 109
To assess whether putative miRNA target genes had inverse expression to miR-935 levels in MB, we correlated expression levels of miR-935 with selected target genes in MB primary specimens. [score:9]
Expression levels of APC, RELN and MYT1 remained unchanged at the mRNA level following transfection (data not shown), although it is possible that miR-935 could regulate expression of APC, RELN and MYT1 at the translational level. [score:8]
Several putative miR-935 targets predicted by TargetScan were up-regulated in MB compared to CD133+ NSCs (Table S5). [score:7]
Candidate miR-935 target genes obtained from MMIA analyses, where miRNA and mRNA expression was inversely-correlated, were ranked according to TargetScan context score, with a context score greater than or equal to −0.4 subsequently selected as the cut-off for validation in the laboratory. [score:7]
Pearson's correlation analysis between miR-935 relative expression levels and expression levels of selected target genes. [score:7]
Significant up-regulation of miR-935 was confirmed by qRT-PCR, with ∼11 000 fold up-regulation of miR-935 in pre-miR-935 transfected cells at 24 h compared to scrambled negative control cells (p<0.01). [score:6]
To evaluate the robustness of miRNA/mRNA predictions identified by the integration of miRNA and mRNA expression data and to gain insight into the potential role of differentially expressed miRNAs in MB, we first focused on the most significantly down-regulated miRNA, miR-935. [score:6]
Significant down-regulation of three of the seven predicted miR-935 target genes was observed at the mRNA level (Figure 3A–D): Solute carrier family 5 member 3 (SLC5A3) (p = 0.0251), Hypothetical protein LOC9778 (KIAA0232) (p = 0.0004) and Zinc Finger AN1-type domain 6 (ZFAND6) (p = 0.0037). [score:6]
Nevertheless, our results with mir-935 target genes indicated that the integration of miRNA and mRNA expression data was a useful approach for the identification of putative miRNA-regulated genes in MB. [score:6]
To address whether miR-935 regulates predicted target genes in MB, transient over -expression of miR-935 in the MB cell line, PER-547, which has 7000 fold decreased levels of miR-935 relative to normal CD133+ NSCs, was performed. [score:6]
Target gene expression levels in pre-miR-935 -transfected samples were normalized to scrambled control levels. [score:5]
Whilst little is known about the role of miR-935-regulated target genes KIAA0232 and ZFAND6, SLC5A3 plays an important role in mammalian osmoregulation [87]. [score:5]
miR-935 expression levels were obtained using Taqman qRT-PCR miRNA assay (Life Technologies) and correlated to selected putative target gene levels using Pearson's correlation. [score:4]
Taken together, experimental validation of several miR-935 regulated target genes in MB cells confirmed the validity of this approach, suggesting that predicted miRNA/mRNA interactions obtained in our study may represent functional miRNA/mRNA networks in MB. [score:4]
Validation of miR-935 and miR-10a target gene regulation in MB. [score:4]
Following small RNA-enriched RNA extraction, significant up-regulation of miR-935 and miR-10a was confirmed by qRT-PCR using two way analysis of variance (ANOVA) and an unpaired t-test, respectively. [score:4]
A moderate to strong negative correlation was observed for the majority of miR-935 target genes, supporting prediction and integration analyses (Table 4). [score:3]
Quantitative RT-PCR analysis of (A) KIAA0232 (B) SLC5A3 (C) TBC1D9 and (D) ZFAND6 expression levels in PER-547 cells 24 h after transfection with scrambled negative control or pre-miR-935. [score:3]
Validation of putative miR-935 targets in PER-547 cells. [score:3]
Validation of putative miR-935 and miR-10a targets in MB. [score:3]
0023935.g003 Figure 3Quantitative RT-PCR analysis of (A) KIAA0232 (B) SLC5A3 (C) TBC1D9 and (D) ZFAND6 expression levels in PER-547 cells 24 h after transfection with scrambled negative control or pre-miR-935. [score:3]
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[+] score: 10
Among the top downregulated, miR-935 previously has been shown to be downregulated in elder hBM-MSCs [81]; miR-193a has been reported to regulate uPA [82], to target oxidative stress pathway [83], and not to be repressed in normal BM cells [84]. [score:10]
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[+] score: 9
For the latent stage, 18 consistently differentially expressed mature miRNA sequences were identified: 8 were up-regulated (miR-212-3p, miR-21-5p, miR-132-3p, miR-20a-5p, miR-17-5p, miR-27a-3p, miR-23a-3p, miR-146a-5p) and 10 were down-regulated (miR-139-5p, miR-551b-3p, miR-33-5p, miR-708-5p, miR-7a-5p, miR-935, miR-138-5p, miR-187-3p, miR-30e-3p, miR-222-3p) (Table  2). [score:9]
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[+] score: 9
miR-935 was 5.6 fold up-regulated in eosinophils compared to mDCs (p* = 0.007). [score:3]
Four small -RNA transcripts were each found to be expressed specifically in one cell type: miR-378 in monocytes, miR-31 in T cells, miR-935 in eosinophils and miR-143 in neutrophils (Figure 1). [score:3]
We identified miR-143 as neutrophil specific, miR-31 as T-cell specific, miR-378 as monocyte specific and miR-935 as eosinophil specific. [score:1]
Little is known regarding the function of miR-378 in monocytes, or miR-935 in eosinophils. [score:1]
All miRNAs reported as specific to single cell types in the Roche dataset (miR-143, miR-31, miR-935 and miR-378) remained significant in the HUG dataset, while additional miRNAs were found to be cell-type specific, most notably miR-145 in neutrophils, miR-181 in NK cells and miR-146 in T cells and NK cells. [score:1]
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[+] score: 5
Interestingly, dichotomization based on the median of 4 miRNAs, -miR-222-5p, miR-27a-5p, miR-125b-3p and miR-935-3p, albeit their expression levels were rather low, suggested that higher miRNA expression level was associated with poor overall survival (Figure S1). [score:5]
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[+] score: 4
Our miRNA profile (84) Chromosomal localization Fold change Reference DOWNREGULATED hsa-miR-206 6p12.2 −7.53(29) hsa-miR-219-2-3p 9q33.3 −6.64(52) hsa-miR-383 8p22 −6.56(12, 55, 56) hsa-miR-138 16q13.3/3p21.32 −5.16(12, 14) hsa-miR-323-3p 14q32.2 −4.96(12, 52) hsa-miR-122 18q21.31 −4.82 hsa-miR-105 Xq28 −4.66 hsa-miR-129-5p 11p11.2/7q32.1 −4.56(23) hsa-miR-935 19q13.43 −4.53(52) hsa-miR-329 14q32.2 −4.48 hsa-miR-129-3p 11p11.2/7q32.1 −4.43 hsa-miR-650 22q11.21 −4.19 hsa-miR-184 15q24.3 −4.14 hsa-miR-370 14q32.2 −3.99(12) hsa-miR-433 14q32.2 −3.96(29) hsa-miR-138-2* 16q13. [score:4]
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[+] score: 4
Other miRNAs from this paper: hsa-let-7b, hsa-mir-15a, hsa-mir-19a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-27a, hsa-mir-28, hsa-mir-30a, hsa-mir-100, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-181a-2, hsa-mir-210, hsa-mir-181a-1, hsa-mir-221, hsa-mir-1-2, hsa-mir-15b, hsa-mir-30b, hsa-mir-122, hsa-mir-132, hsa-mir-141, hsa-mir-191, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125a, hsa-mir-195, hsa-mir-200c, hsa-mir-1-1, hsa-mir-30c-1, hsa-mir-34b, hsa-mir-34c, hsa-mir-30e, hsa-mir-371a, hsa-mir-372, hsa-mir-373, hsa-mir-375, hsa-mir-151a, hsa-mir-429, hsa-mir-449a, hsa-mir-483, hsa-mir-193b, hsa-mir-520e, hsa-mir-520f, hsa-mir-520a, hsa-mir-520b, hsa-mir-520c, hsa-mir-520d, hsa-mir-520g, hsa-mir-520h, hsa-mir-548a-1, hsa-mir-548b, hsa-mir-548a-2, hsa-mir-548a-3, hsa-mir-548c, hsa-mir-548d-1, hsa-mir-548d-2, hsa-mir-449b, hsa-mir-151b, hsa-mir-320b-1, hsa-mir-320b-2, hsa-mir-891a, hsa-mir-1233-1, hsa-mir-548e, hsa-mir-548j, hsa-mir-548k, hsa-mir-548l, hsa-mir-548f-1, hsa-mir-548f-2, hsa-mir-548f-3, hsa-mir-548f-4, hsa-mir-548f-5, hsa-mir-548g, hsa-mir-548n, hsa-mir-548m, hsa-mir-548o, hsa-mir-548h-1, hsa-mir-548h-2, hsa-mir-548h-3, hsa-mir-548h-4, hsa-mir-1275, hsa-mir-548p, hsa-mir-548i-1, hsa-mir-548i-2, hsa-mir-548i-3, hsa-mir-548i-4, hsa-mir-1973, hsa-mir-548q, hsa-mir-548s, hsa-mir-548t, hsa-mir-548u, hsa-mir-548v, hsa-mir-548w, hsa-mir-548x, hsa-mir-1233-2, hsa-mir-548y, hsa-mir-548z, hsa-mir-548aa-1, hsa-mir-548aa-2, hsa-mir-548o-2, hsa-mir-548h-5, hsa-mir-548ab, hsa-mir-548ac, hsa-mir-548ad, hsa-mir-548ae-1, hsa-mir-548ae-2, hsa-mir-548ag-1, hsa-mir-548ag-2, hsa-mir-548ah, hsa-mir-548ai, hsa-mir-548aj-1, hsa-mir-548aj-2, hsa-mir-548x-2, hsa-mir-548ak, hsa-mir-548al, hsa-mir-548am, hsa-mir-548an, hsa-mir-371b, hsa-mir-548ao, hsa-mir-548ap, hsa-mir-548aq, hsa-mir-548ar, hsa-mir-548as, hsa-mir-548at, hsa-mir-548au, hsa-mir-548av, hsa-mir-548aw, hsa-mir-548ax, hsa-mir-548ay, hsa-mir-548az, hsa-mir-548ba, hsa-mir-548bb, hsa-mir-548bc
Overall, six miRNAs were downregulated in both conditions: hsa-miR-151-5p that is related to aging; hsa-miR-935 and hsa-miR-125a-3p that are related to cell cycle and chromatin modification; hsa-miR-132-5p, hsa-miR-320b and hsa-miR-195-5p that are involved in cell and cellular component morphogenesis [47]. [score:4]
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[+] score: 3
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-18a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-21, hsa-mir-23a, hsa-mir-31, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-96, hsa-mir-98, hsa-mir-99a, hsa-mir-106a, mmu-let-7g, mmu-let-7i, mmu-mir-23b, mmu-mir-99a, mmu-mir-127, mmu-mir-128-1, mmu-mir-136, mmu-mir-142a, mmu-mir-145a, mmu-mir-10b, mmu-mir-182, mmu-mir-183, mmu-mir-187, mmu-mir-193a, mmu-mir-195a, mmu-mir-200b, mmu-mir-206, mmu-mir-143, hsa-mir-139, hsa-mir-10b, hsa-mir-182, hsa-mir-183, hsa-mir-187, hsa-mir-210, hsa-mir-216a, hsa-mir-217, hsa-mir-219a-1, hsa-mir-221, hsa-mir-222, hsa-mir-224, hsa-mir-200b, mmu-mir-302a, mmu-let-7d, mmu-mir-106a, hsa-let-7g, hsa-let-7i, hsa-mir-23b, hsa-mir-128-1, hsa-mir-142, hsa-mir-143, hsa-mir-145, hsa-mir-127, hsa-mir-136, hsa-mir-193a, hsa-mir-195, hsa-mir-206, mmu-mir-19b-2, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-18a, mmu-mir-21a, mmu-mir-23a, mmu-mir-31, mmu-mir-92a-2, mmu-mir-96, mmu-mir-98, hsa-mir-200c, mmu-mir-17, mmu-mir-139, mmu-mir-200c, mmu-mir-210, mmu-mir-216a, mmu-mir-219a-1, mmu-mir-221, mmu-mir-222, mmu-mir-224, mmu-mir-19b-1, mmu-mir-92a-1, mmu-mir-128-2, hsa-mir-128-2, mmu-mir-217, hsa-mir-200a, hsa-mir-302a, hsa-mir-219a-2, mmu-mir-219a-2, hsa-mir-363, mmu-mir-363, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-371a, hsa-mir-18b, hsa-mir-20b, hsa-mir-452, mmu-mir-452, ssc-mir-106a, ssc-mir-145, ssc-mir-216-1, ssc-mir-217-1, ssc-mir-224, ssc-mir-23a, ssc-mir-183, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-128-1, ssc-mir-136, ssc-mir-139, ssc-mir-18a, ssc-mir-21, hsa-mir-146b, hsa-mir-493, hsa-mir-495, hsa-mir-497, hsa-mir-505, mmu-mir-20b, hsa-mir-92b, mmu-mir-302b, mmu-mir-302c, mmu-mir-302d, hsa-mir-671, mmu-mir-216b, mmu-mir-671, mmu-mir-497a, mmu-mir-495, mmu-mir-146b, mmu-mir-708, mmu-mir-505, mmu-mir-18b, mmu-mir-493, mmu-mir-92b, hsa-mir-708, hsa-mir-216b, hsa-mir-302e, hsa-mir-302f, ssc-mir-17, ssc-mir-210, ssc-mir-221, mmu-mir-1839, ssc-mir-146b, ssc-mir-206, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-128-2, ssc-mir-143, ssc-mir-10b, ssc-mir-23b, ssc-mir-193a, ssc-mir-99a, ssc-mir-98, ssc-mir-92a-2, ssc-mir-92a-1, ssc-mir-92b, ssc-mir-142, ssc-mir-497, ssc-mir-195, ssc-mir-127, ssc-mir-222, ssc-mir-708, ssc-mir-935, ssc-mir-19b-2, ssc-mir-19b-1, ssc-mir-1839, ssc-mir-505, ssc-mir-363-1, hsa-mir-219b, hsa-mir-371b, ssc-let-7a-2, ssc-mir-18b, ssc-mir-187, ssc-mir-218b, ssc-mir-219a, mmu-mir-195b, mmu-mir-145b, mmu-mir-21b, mmu-let-7j, mmu-mir-21c, ssc-let-7d, ssc-let-7f-2, ssc-mir-20b-1, ssc-mir-20b-2, ssc-mir-31, ssc-mir-182, ssc-mir-216-2, ssc-mir-217-2, ssc-mir-363-2, ssc-mir-452, ssc-mir-493, ssc-mir-671, mmu-let-7k, ssc-mir-7138, mmu-mir-219b, mmu-mir-216c, mmu-mir-142b, mmu-mir-497b, mmu-mir-935, ssc-mir-9843, ssc-mir-371, ssc-mir-219b, ssc-mir-96, ssc-mir-200b
Ssc-miR-106a, ssc-miR-363, ssc-miR-195, ssc-miR-497, ssc-miR-146b, ssc-miR-92b-5p, ssc-miR-20b and ssc-miR-935 were highly expressed in hpiPSCs than that in mpiPSCs (Fig 3A). [score:3]
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[+] score: 1
The number of CCAAT/ATTGG matrices identified varies from a minimum of 3 (pre-miRNA-1302-2, -1302-10) to a maximum of 160 (pre-miRNA-935) while on the 80% of miRNA genomic loci occur 50–100 CCAAT/ATTGG matrices (Fig 2A). [score:1]
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[+] score: 1
Meanwhile, we also revealed several novel miRNAs with so far unknown biological functions in lung tumorigenesis, such as miR-934, miR-935 and miR-936. [score:1]
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