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22 publications mentioning tae-MIR399

Open access articles that are associated with the species Triticum aestivum and mention the gene name MIR399. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 41
The expression of TaPHO2 was presented as percentage of that in the control leaves which were transformed with TaPHO2-A1, -B1 or -D1 alone, the relative expression levels of tae-miR399-A1 and TaIPS1 were normalized using the expression of NtACTIN. [score:7]
The relative expression levels of TaPHO2 (a), tae-miR399-A1 (b) and TaIPS1 (c) in the tobacco leaves transiently overexpressed the indicated gene(s). [score:5]
Previously, the miR399 activity in cleaving PHO2 has been found to be reduced by IPS1 according to the target mimicry mechanism in Arabidopsis 14. [score:3]
Each of them contained 10 exons (including 2 untranslated exons in the 5′-UTR), and had five putative miR399 -binding sites in the second exon (Fig. 1a). [score:3]
The degradation of the TaPHO2s by tae-miR399-A1 was able to be inhibited by TaIPS1.1 (Fig. 2). [score:3]
These results indicated that all the three TaPHO2 genes were able to be degraded by tae-miR399, and TaIPS1.1 inhibited this degradation. [score:3]
Indeed, it has been reported that the miR399- PHO2 module regulates the flowering time in response to different ambient temperatures in Arabidopsis 30. [score:2]
The regulation of the TaPHO2 transcripts by tae-miR399 and TaIPS1. [score:2]
Regulation of TaPHO2 by tae-miR399 and TaIPS1. [score:2]
Regulation of TaPHO2 by tae-miR399 and TaIPS1Sequence analysis revealed that there were five putative miR399 -binding sites in 5′-UTRs of the three TaPHO2 genes (Supplemental Fig. S4). [score:2]
As such, the IPS1-miR399- PHO2 signaling cascade is conserved in plants. [score:1]
As all the three TaIPS1 genes conferred the conserved complementary sequences with tae-miR399, only TaIPS1.1 was chosen to check if it affected the degradation of TaPHO2-A1, -B1 or -D1 by tae-miR399. [score:1]
When TaPHO2-A1, -B1 or -D1 was co-transformed with tae-miR399-A1 in the tobacco leaves, the mRNA levels of all three TaPHO2 genes were significantly lower than that transformed with TaPHO2 gene alone (Fig. 2a). [score:1]
Sequence analysis revealed that there were five putative miR399 -binding sites in 5′-UTRs of the three TaPHO2 genes (Supplemental Fig. S4). [score:1]
In order to investigate whether TaPHO2 could be degraded by tae-miR399, we used tobacco transient expression system to analyze this possibility. [score:1]
Moreover, sequence analysis of TaIPS1s also found that three TaIPS1 genes all contained a motif with sequence complementarity to tae-miR399 (Supplemental Fig. S4). [score:1]
TaPHO2 mRNA levels in the tobacco leaves transformed with TaPHO2, tae-miR399-A1 and TaIPS1.1 were higher than that transformed with TaPHO2 and tae-miR399-A1 (Fig. 2a). [score:1]
The full length of tae-miR399 and TaIPS1.1 were also cloned into pRI101-AN vector. [score:1]
The blue ticks in the second exon depict the position of five putative miR399 binding sites. [score:1]
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2
[+] score: 27
The target of this miR399 is the mRNA encoding the enzyme “Phosphatase 2” (PHO2), and downregulation of PHO2 was shown in barley by Hackenberg and his colleagues when the miR399 was upregulated (Hackenberg et al. 2013a). [score:9]
Wang and collogues identified induced expression of miR159 and miR399 upon wounding while miR164, miR167, miR393, and miR398 were detected as downregulated by the same process. [score:6]
P starvation-responsive miRNA, miR399, was found to be upregulated in both barley and wheat (Zhao et al. 2013; Hackenberg et al. 2013b) (Table 3). [score:4]
They observed that miR159, miR160, miR164, miR399, miR1117, and miR1120 exhibited differential expression suggesting a role in N homeostasis (Sinha et al. 2015) (Table 3). [score:3]
dicocoides Cu–Zn super oxide dismutaseKantar et al. 2011a miR399 H. vulgare No targetLv et al. 2012 miR408 T. turgidum ssp. [score:3]
miR399 is not the only miRNA detected as Pi deficiency responsive. [score:1]
These observations suggest that miR399 is a candidate for manipulation of the Pi uptake pathway in wheat and barley. [score:1]
[1 to 20 of 7 sentences]
3
[+] score: 24
According to genome sequencing information, SIZ1 is conserved in rice [27]; miR399 expression has been confirmed in rice, and a potential orthologue of UBC24/PHO2 has been identified by assembly using genomic DNA and expressed sequence tags (ESTs) [26]. [score:5]
Comparison of upregulated wheat and rice transcripts revealed that the PHR1-IPS1-miR399-UBC24/PHO2 signalling cascade is conserved in both crop species. [score:4]
Based on the identification of HvmiR399s [28] and the upregulation of HvIPS1[29], the IPS1-miR399-UBC24/PHO2 signalling cascade appears to be conserved in barley [18]. [score:4]
IPS1, a non-protein coding gene that includes highly conserved sequences near miR399 complementary regions in different plant species [22], has been found to be strongly upregulated under −P in rice [10]. [score:4]
These results suggested that the PHR1-IPS1-miR399-UBC24/PHO2 signalling cascade functions as a potent adaptation to −P in wheat. [score:1]
In Arabidopsis, SIZ1 (small ubiquitin-like modifier [SUMO] E3 ligase) [16], miR399, and UBC24/PHO2 (ubiquitin E2 conjugase) are also involved in the cascade (Figure 5) [26]. [score:1]
A highly conserved PHR1-IPS1-miR399-UBC24/PHO2 signalling cascade. [score:1]
Consequently, the PHR1-IPS1-miR399-UBC24/PHO2 signalling cascade is probably also conserved in wheat (Figure 5). [score:1]
These factors may be related to the PHR1-IPS1-miR399-UBC24/PHO2 signalling cascade, as Pi remobilisation, Pi uptake, and Pi-related metabolism are under the control of that cascade (Figure 5). [score:1]
Members of the IPS1 gene family from different plant species, including rice, have a highly-conserved 23-nt-long motif that exhibits complementarity with the miR399 involved in Pi response [22]. [score:1]
Transcribed sequences encoding miR399, miR399 -binding sites, or protein sequences homologous to N- or C-terminal extensions of UBC24/PHO2 have been observed in wheat [26]. [score:1]
[1 to 20 of 11 sentences]
4
[+] score: 15
P accumulation and leaf necrosis also occurred in the Osspx3 and Osspx5 double mutant, and the expression levels of PSI genes, including IPS1, miR399, PT2, miR827, PAP10 and SQD2, were significantly upregulated [46]. [score:6]
PHR1 regulates the expression of AtACP5, AtIPS1, PHT1.1 and RNS1 [39, 40], as well as the expression of several PSR genes, including microRNA399 and the SPX genes [38], by binding to their promoters through the cis-element PHR1 -binding sequence (P1BS; GNATATNC) [40– 42]. [score:5]
PHR2, SPX1 and SPX2 constitute a regulatory feedback loop in P signallingWang et al. [28, 45] OsSPX3 N/C + NrOsSPX3 plays an important role in OsIPS1/miR399 -mediated long distance regulation on OsPHO2 and acts as a negative regulator of OsPHR2. [score:4]
[1 to 20 of 3 sentences]
5
[+] score: 13
Upon Pi starvation, miR156, miR399, miR778, miR827, and miR2211 of Arabidopsis (Arabidopsis thaliana) display an upregulated expression pattern (Fujii et al., 2005). [score:6]
Characterization on miR399 in more detail has elucidated the mechanism underlying the miRNA/target module -mediated internal Pi homeostasis; miR399 regulates Pi starvation response through its role in modifying the target PHO2 transcripts, which further impact Pi uptake and internal P translocation across tissues via transcriptionally regulation of the PT genes (Fujii et al., 2005; Chiou et al., 2006; Pant et al., 2009). [score:5]
miR399 mediates plant cellular Pi homeostasis to be associated with its transcriptional regulation of a set of PT genes, such as Pht1;8 and Pht1;9 (Bari et al., 2006; Chiou et al., 2006; Sunkar et al., 2012). [score:2]
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6
[+] score: 9
In contrast, expression of miR167 and miR396 isomiRs was induced by heat stress (Fig. S5d,e), while expression of three of the four miR399 isomiRs was upregulated from 3 to 10 DAT (Fig. S5f). [score:8]
Families miR167, miR169 and miR398 were represented by variants of five to seven of the length between 18 to 24-mers while miRNAs from miR399 and miR5205 were all 21 nt-long (Fig. 2b). [score:1]
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7
[+] score: 8
For instance, sequestration of miR-399 by target mimic IPS1 lncRNA resulted in increase in the accumulation of target PHO2 mRNA in Arabidopsis (Franco-Zorrilla et al., 2007). [score:5]
In plants, lncRNA Induced by Phosphate Starvation 1 (IPS1) which interferes in binding of ath-miR399 to its specific target, was first discovered in Arabidopsis (Franco-Zorrilla et al., 2007). [score:3]
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8
[+] score: 8
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR162a, osa-MIR164a, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR394, osa-MIR395b, osa-MIR395d, osa-MIR395e, osa-MIR395g, osa-MIR395h, osa-MIR395i, osa-MIR395j, osa-MIR395k, osa-MIR395l, osa-MIR395s, osa-MIR395t, osa-MIR395c, osa-MIR395a, osa-MIR395f, osa-MIR395u, osa-MIR396a, osa-MIR396b, osa-MIR396c, osa-MIR397a, osa-MIR397b, osa-MIR398a, osa-MIR398b, osa-MIR399a, osa-MIR399b, osa-MIR399c, osa-MIR399d, osa-MIR399e, osa-MIR399f, osa-MIR399g, osa-MIR399h, osa-MIR399i, osa-MIR399j, osa-MIR399k, osa-MIR156k, osa-MIR156l, osa-MIR159b, osa-MIR162b, osa-MIR166k, osa-MIR166l, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR168a, osa-MIR168b, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR408, osa-MIR172d, osa-MIR167j, osa-MIR166m, osa-MIR166j, osa-MIR437, osa-MIR396e, osa-MIR444a, osa-MIR528, osa-MIR529a, osa-MIR395m, osa-MIR395n, osa-MIR395o, osa-MIR395p, osa-MIR395q, osa-MIR395v, osa-MIR395w, osa-MIR395r, osa-MIR529b, tae-MIR159b, tae-MIR167a, tae-MIR408, tae-MIR444a, osa-MIR1432, osa-MIR444b, osa-MIR444c, osa-MIR444d, osa-MIR444e, osa-MIR444f, osa-MIR1848, osa-MIR1858a, osa-MIR1858b, osa-MIR1862a, osa-MIR1862b, osa-MIR1862c, osa-MIR1871, osa-MIR1862d, osa-MIR1862e, osa-MIR827, osa-MIR396f, osa-MIR396g, osa-MIR396h, osa-MIR396d, osa-MIR395x, osa-MIR395y, hvu-MIR156a, tae-MIR156, hvu-MIR159b, hvu-MIR166a, tae-MIR167b, hvu-MIR168, tae-MIR395a, tae-MIR395b, hvu-MIR397a, tae-MIR398, tae-MIR444b, hvu-MIR166b, hvu-MIR444a, osa-MIR1862f, osa-MIR1862g, hvu-MIR399, hvu-MIR444b, hvu-MIR166c, tae-MIR396, tae-MIR167c, tae-MIR397, hvu-MIR397b, hvu-MIR156b
Hence, these results confirm actual expression of pre-miR396, pre-miR399 and pre-miR827. [score:3]
miR399 and miR827 are both important for plant uptake of phosphorus under phosphorus deficiency [62], while miR396 is important in controlling cell proliferation [63]. [score:1]
Figure 5 Sequence alignment of each of pre-miRNA396, pre-miRNA399 and pre-miRNA827 from rice, Brachypodium and/or wheat. [score:1]
To test the validity of this approach, we first selected three conserved miRNAs, namely miR396, miR399 and miR827. [score:1]
Sequencing of these PCR products showed high sequence similarity of the barley pre-miRNAs with those from rice, Brachypodium or wheat (Figure 5), confirming that they are indeed barley pre-miR396, pre-miR399 and pre-miR827. [score:1]
To identify pre-miR396, pre-miR399 and pre-miR827 in barley, sequences of pre-miRNA sequences from rice, Brachypodium and/or wheat were aligned to determine conserved regions (Figure 5). [score:1]
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9
[+] score: 6
The results also showed that miR399 targeted putative disease resistance RPP13-like protein, a glycine-rich RNA -binding protein involved in embryo sac development. [score:6]
[1 to 20 of 1 sentences]
10
[+] score: 6
Other miRNAs from this paper: tae-MIR159a, tae-MIR159b
Therefore, IPS1 functions as noncleavable target mimic of miR399 [14]. [score:3]
Non-protein-coding RNA INDUCED BY PHOSPHATE STARVATION1 (IPS1), partially basepairing to miR399 with a three-nucleotide mismatch at miR399 cleavage site, can sequester miR399 and arrest its cleavage activity to its target PHOSPHATE2 mRNA. [score:3]
[1 to 20 of 2 sentences]
11
[+] score: 5
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR160a, osa-MIR160b, osa-MIR160c, osa-MIR160d, osa-MIR164a, osa-MIR164b, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR169a, osa-MIR171a, osa-MIR393a, osa-MIR396a, osa-MIR396b, osa-MIR396c, osa-MIR397a, osa-MIR397b, osa-MIR399a, osa-MIR399b, osa-MIR399c, osa-MIR399d, osa-MIR399e, osa-MIR399f, osa-MIR399g, osa-MIR399h, osa-MIR399i, osa-MIR399j, osa-MIR399k, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR319a, osa-MIR319b, osa-MIR160e, osa-MIR160f, osa-MIR164c, osa-MIR164d, osa-MIR164e, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR168a, osa-MIR168b, osa-MIR169b, osa-MIR169c, osa-MIR169d, osa-MIR169e, osa-MIR169f, osa-MIR169g, osa-MIR169h, osa-MIR169i, osa-MIR169j, osa-MIR169k, osa-MIR169l, osa-MIR169m, osa-MIR169n, osa-MIR169o, osa-MIR169p, osa-MIR169q, osa-MIR171b, osa-MIR171c, osa-MIR171d, osa-MIR171e, osa-MIR171f, osa-MIR171g, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR171h, osa-MIR393b, osa-MIR408, osa-MIR172d, osa-MIR171i, osa-MIR167j, osa-MIR164f, osa-MIR390, osa-MIR439a, osa-MIR439b, osa-MIR439c, osa-MIR439d, osa-MIR439e, osa-MIR439f, osa-MIR439g, osa-MIR439h, osa-MIR439i, osa-MIR396e, osa-MIR444a, tae-MIR159a, tae-MIR159b, tae-MIR160, tae-MIR164, tae-MIR167a, tae-MIR171a, tae-MIR408, tae-MIR444a, osa-MIR169r, osa-MIR444b, osa-MIR444c, osa-MIR444d, osa-MIR444e, osa-MIR444f, osa-MIR396f, osa-MIR396g, osa-MIR396h, osa-MIR396d, tae-MIR156, tae-MIR319, tae-MIR167b, tae-MIR169, tae-MIR444b, tae-MIR171b, tae-MIR396, tae-MIR167c, tae-MIR397
The frequencies of the miRNA families varied from 2 (miR390, miR396, miR397, miR399) to 757 (miR169), indicating that expression varies highly among the different miRNA families in wheat (Figure 2). [score:3]
These include miRNA156/157, miR159, miR160, miR164, miR165/166, miR167, miR168, miR169, miR170/171, miR172, miR319, miR390, miR393, miR396, miR397, miR399 and miR408, which are conserved in diverse plant species (Table 2). [score:1]
This analysis revealed perfect matching of nine miRNA families, miR159, miR160, miR164, miR167, miR169, miR170, miR399, miR408 and miR444, to 14 ESTs. [score:1]
[1 to 20 of 3 sentences]
12
[+] score: 5
Specific expression of tae-miR399 was found in the control sample, whereas tae-miR5085, tae-miR5200, and tae-miR6197-5p were specifically expressed in the drought sample. [score:5]
[1 to 20 of 1 sentences]
13
[+] score: 4
We also found 8 cases (miR156, miR159, miR172, miR167, miR169, miR396, miR399 and miR818) where all the members of a miRNA family were expressed at similar pattern in response to powdery mildew infection or heat stress. [score:3]
Recent studies in Arabidopsis have also established that miR399, miR395 and miR398 are induced in response to phosphate-, sulfate- and Cu [2+]-deprived conditions, respectively [8, 26- 29]. [score:1]
[1 to 20 of 2 sentences]
14
[+] score: 4
Other miRNAs from this paper: bdi-MIR399a, hvu-MIR399, bdi-MIR399b, bdi-MIR399c, bdi-MIR399d
The AtIPS1 transcript contains a miR399 recognition site and functions as a miRNA target mimic [30]. [score:3]
A 251 bp fragment of HvIPS1, downstream of the miR399 recognition site, was inserted into BSMV-γ-MCS, and seven days old plants grown under hydroponics conditions as described above were inoculated with BSMV-α, -β and either BSMV-γ-IPS1 or a BSMV-γ-GFP [250 ]control construct. [score:1]
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15
[+] score: 3
Two microRNAs, tae-miR399 and tae-miR9778, were specifically expressed in SL1 (Fig. 4c). [score:3]
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16
[+] score: 3
In another example, lncRNA IPS1 has been shown to inhibit miR399 -mediated cleavage of PHO2 as a competitor for PHO2 mRNA [24]. [score:3]
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17
[+] score: 3
In plants, after the IPS1-target mimic of miR399 was identified, Wu et al. [42] predicted endogenous mimics (eTMs) for 20 conserved miRNAs from intergenic or non-coding gene-originated regions in Arabidopsis and rice, and several Arabidopsis eTMs have been shown to be functional [41, 42, 52]. [score:3]
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18
[+] score: 3
Other miRNAs from this paper: tae-MIR396
To generate the artificial MIM172, we synthesized the wheat ortholog of the natural miR399 target mimic IPS1 gene described in Arabidopsis (Franco-Zorrilla et al., 2007) and replaced the sequence complementary to miR399 by a sequence complementary to miR172. [score:3]
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19
[+] score: 2
Other miRNAs from this paper: tae-MIR167a, tae-MIR167b, tae-MIR169, tae-MIR167c
Three other miRNA families with well-established roles in plants, miR167, miR169 and miR399, were also prominent (10.1%, 6.9% and 7.8%, respectively) among miRNAs putatively encoded by Tdic5B. [score:1]
Additionally, Tdic5B was found to encode 11 members of miR167 family, 10 members of miR169 family and 6 members of miR399 family. [score:1]
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20
[+] score: 1
Eleven miRNA variants were more abundant and substituted for the reported miRNA sequences such as miR156a, miR399 and miR408 (S3 and S4 Tables). [score:1]
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21
[+] score: 1
Of these miR437, miR2118, miR2905, miR5049, miR5050, miR5075 & miR5200 have not previously been reported from wheat EST sequences, while miR399k is also a new representative of the miR399 family. [score:1]
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22
[+] score: 1
Sixteen miRNA families were shown to be putatively present on chromosome 5A: two of them (miR164 and miR167) were found only in the short arm, three families (miR156, miR399 and miR2118) were found only in the long arm, while the remaining eleven families were found in both arms (Table 3). [score:1]
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