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6 publications mentioning hsa-mir-1237

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-1237. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 22
To verify that the up-regulation of these miRNAs was consequent to DNA demethylation after 5-aza-dC treatment, we assessed changes in DNA methylation status in the proximal regions of miR-941-1/3, miR-1237 and miR-1247 by bisulfite genomic sequencing (Figure 2). [score:4]
Over -expression of miR-941, miR-1237, and miR-1247 in HCT116, D KO, and DLD1 cells was achieved by transfecting the cells with 20 nM miRNA duplexes that mimicked the mature miR-941 (MSY0004984, Qiagen), miR-1237 (MSY0005592, Qiagen), and miR-1247 (MSY0005899, Qiagen). [score:3]
To determine if expression of these epigenetically silenced miRNAs would affect cancer cell growth and migration, we transfected HCT116 cells with miRNA mimics of miR-941 (the mature form of miR-941-1 and -3), miR-1237, miR-1247, and a non-coding negative control (AllStars Neg. [score:3]
Hence, we tested the effects of ectopic expression of miR-941, miR-1237, and miR-1247 on cell mobility in HCT116 cells. [score:3]
We focused on two important aspects of cancer development, growth and migration, and selected miR-941, miR-1237, and miR-1247 for functional studies. [score:2]
Figure S4 Functional analysis of miR-941, miR-1237, and miR-1247 in DLD1 cells. [score:1]
Functional analysis of miR-941, miR-1237, and miR-1247 in HCT116 cells. [score:1]
0020628.g002 Figure 2Bisulfite sequencing was carried out for (A) miR-941, (B) miR-1237, and (C) miR-1247 in parental HCT116 cells, HCT116 cells treated with 5 µM 5-aza-dC for 48 hr, and D KO cells. [score:1]
Based on qRT-PCR results, we determined that miR-1237, miR-602, miR-941-1, miR-941-3, and miR-663b are transcriptionally independent from their respective putative host genes (Figure 4). [score:1]
UCSC genome browser screen captures of the promoter regions (±1000 bp from annotated transcription start site) for (A) RPS6KA4, putative host gene for miR-1237, (B) C9orf3, putative host gene for miR-24-1 and miR-27b, (C) AB058779, putative host gene for miR-602, (D) ANKRD30BL, putative host gene for miR-663b, and (E) DNAJC5, putative host gene for miR-941-1 and miR-941-3. The first track contains DNA methylation at these promoters in HCT116, and the second track contains DNA methylation in D KO cells as detected by MiGS. [score:1]
), miR-941, miR-1237, or miR-1247 mimics. [score:1]
was carried out for (A) miR-941, (B) miR-1237, and (C) miR-1247 in parental HCT116 cells, HCT116 cells treated with 5 µM 5-aza-dC for 48 hr, and D KO cells. [score:1]
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2
[+] score: 7
We selected 12 miRNAs: miR-9, miR-19b, miR-27b, miR-92a, miR-140-5p, miR-190, miR-200a, let-7a, miR-129-5p, miR-582-5p, miR-892a, and miR-1237 (Figure  1) and tested whether they downregulate FOXP2 expression in cell culture systems. [score:6]
In contrast, miR-27b, miR-92a, miR-190, and miR-1237 did not repress luciferase activity whether the reporter constructs contained a FOXP2 3′ UTR or not (Figure  3A). [score:1]
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3
[+] score: 7
Functional screening by in vitro transfection of miRNA mimics and inhibitorsMiRNA mimics for miR-1237, miR-365b-5p, miR-550a-5p and miR-135a-3p and miRNA inhibitors for miR-301b, miR-503, miR-542-5p and miR-210 were chosen for further functional investigation. [score:3]
We performed GO analysis and KEGG pathway analysis on the differentially expressed miRNAs, and the results are shown in Supplementary Figure 1. Ultimately, fifteen potential miRNAs associated with the proliferation of osteosarcoma were selected for microarray validation by quantitative real-time RT-PCR analysis, including miR-1237, miR-550a-5p, miR-365b-5p, miR-135a-3p, miR-933, miR-762, miR-629-3p, miR-542-5p, miR-503, miR-301b, miR-210, miR-374a-5p, miR-199a-5p, miR-199a-3p and miR-195-5p. [score:3]
MiRNA mimics for miR-1237, miR-365b-5p, miR-550a-5p and miR-135a-3p and miRNA inhibitors for miR-301b, miR-503, miR-542-5p and miR-210 were chosen for further functional investigation. [score:1]
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4
[+] score: 4
Using, miR-1237, miR-30a, miR-598, miR-601, miR-205*, and miR-328 exhibited enrichment of seed regions in the set of mRNAs up-regulated 20 hours after IR. [score:4]
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5
[+] score: 3
Of the original list of 45 overexpressed microRNAs, 14 had no specific-3p or-5p strand information and a further microRNA (miR-1237-3p) had no corresponding-5p probe on the test platform (Supplementary Table S1). [score:3]
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6
[+] score: 1
In addition, Yan et al. (2011) performed a genome-wide methylome analysis entailing deep sequencing of MBD (methylated DNA binding domain)-isolated DNA in HCT116 cells, and identified a number of methylated genes, including miR-941, miR-1237, and miR-1247. [score:1]
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