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1 publications mentioning bmo-mir-2761

Open access articles that are associated with the species Bombyx mori and mention the gene name mir-2761. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 137
In the present study, we used qRT-PCR to detect the expression of Bmo-miR-2761-3p and BmSDH, result show that Bmo-miR-2761-3p was significantly down-regulated after HCl acid treated, however BmSDH mRNA was significantly up-regulated, suggesting that BmSDH mRNA expression was closely related to Bmo-miR-2761-3p. [score:11]
Bmo-miR-2761-3p down-regulated the expression of BmSDH gene in vitroTarget prediction (S2 Fig) and the qRT-PCR result were indicated that BmSDH might be one of the target genes of Bmo-miR-2761-3p. [score:10]
Student t-test analysis showed that the expression difference was statistically significant (p<0.01), indicating that Bmo-miR-2761-3p could down-regulate the expression of BmSDH in vitro. [score:8]
The up-regulation of BmSDH was closely associated with the down-regulation of Bmo-miR-2761-3p (R [2] = 0.96). [score:7]
Expressions pattern of Bmo-miR-2761-3p and BmSDHSince the BmSDH gene play an important role in the B. mori diapause, we interesting in which miRNAs would regulated its expression. [score:6]
Bmo-miR-2761-3p down-regulated the expression of BmSDH gene in vitro. [score:6]
Target prediction (S2 Fig) and the qRT-PCR result were indicated that BmSDH might be one of the target genes of Bmo-miR-2761-3p. [score:5]
For further research, we used DLR assay to confirmed that Bmo-miR-2761-3p could down-regulate BmSDH expression. [score:5]
GO classification and KEGG pathway enrichment analysis of candidate target genes were used to detect the expression of Bmo-miR-2761-3p and Bombyx mori sorbitol dehydrogenase(BmSDH). [score:5]
We hypothesized that high concentration of Bmo-miR-2761-3p might inhibit BmSDH expression, maintaining diapause stage. [score:5]
qRT-PCR and the DLR assay results indicated Bmo-miR-2761-3p can downregulated the BmSDH expression. [score:5]
These results indicated that Bmo-miR-2761-3p may suppressed BmSDH expression. [score:5]
0180085.g007 Fig 7 Bmo-miR-2761-3p down-regulated BmSDH in vitro. [score:4]
Bmo-miR-2761-3p down-regulated BmSDH in vitro. [score:4]
In the present study, to investigate the regulatory function in silkworm diapause termination, used the DE miRNAs as candidate, by the target prediction, we found only Bmo-miR-2761-3p have target position in the BmSDH 3’UTR. [score:4]
With HCl treatment, Bmo-miR-2761-3p was down-regulated and BmSDH was activated, which then started glycogen synthesis and mediated changes of metabolism to promote embryogenesis. [score:4]
The predicted of Bmo-miR-2761-3p target in BmSDH. [score:3]
After a 24-hour co-transfection, the firefly luciferase activity of Bmo-miR-2761-3p mimics -treated cells was weaker, approximately 89.6% lower than NC mimics -treated cells in the PC group; while in the WT group, Bmo-miR-2761-3p mimic binding BmSDH 3’UTR lowered the firefly luciferase expression by 32.4% (Fig 7). [score:3]
However, compared to the control eggs, the relative Bmo-miR-2761-3p transcript level in HCl -treated eggs was only 0.220, a 76.01% down-regulation 1 day after HCl treatment. [score:3]
By the qRT-PCR screening in day 3 to 7 old eggs, however, Bmo-miR-2761-3p and BmSDH expressed differently and have a strong negative correlation. [score:3]
Expressions pattern of Bmo-miR-2761-3p and BmSDH. [score:3]
0180085.g006 Fig 6Expressions pattern of Bmo-miR-2761-3p and BmSDH from 3 to 7 day old eggs of silkworm, B. mori. [score:3]
BmSDH 3’UTR (746 bp) was cloned and inserted into the Luc2 3’UTR to construct wild type (WT) plasmid, while Bmo-miR-2761-3p inhibitor was inserted into Luc2 3’UTR to construct positive control (PC) plasmid. [score:3]
PC group represents cells transfected with positive control plasmid (Bmo-miR-2761-3p inhibitor inserted into Luc2 3’UTR), WT group represents cells transfected with plasmid containing BmSDH 3’UTR. [score:3]
Expressions pattern of Bmo-miR-2761-3p and BmSDH from 3 to 7 day old eggs of silkworm, B. mori. [score:3]
The expression of Bmo-miR-2761-3p rose slightly afterwards, but maintained at a relatively low level (Fig 6A). [score:3]
Student t-test analysis showed that the gene expression was significantly different between HCl -treated eggs and control eggs for both Bmo-miR-2761-3p and BmSDH (p<0.01). [score:3]
Bmo-miR-2761-3p was identified as an inhibitor of BmSDH expression using (Dual luciferase reporter assay) DLR assay. [score:3]
S2 FigThe predicted of Bmo-miR-2761-3p target in BmSDH. [score:3]
“NC” represents cells transfected with the negative control mimics, “mimics” represents cells transfected with Bmo-miR-2761-3p mimics. [score:1]
Bmo-miR-2761-3p mimics and negative control (NC) mimics were purchased from Dingguo (Beijing, China). [score:1]
Co-transfection was classified into two groups: PC group, including PC + NC mimics and PC + Bmo-miR-2761-3p mimics, and WT group, including WT + NC mimics and WT + Bmo-miR-2761-3p mimics. [score:1]
The first strand and specific cDNAs were used as templates in the amplification of BmSDH (gb|DQ443393.1) and Bmo-miR-2761-3p (MIMAT0013635). [score:1]
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