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1 publications mentioning tgu-mir-29a-1Open access articles that are associated with the species Taeniopygia guttata and mention the gene name mir-29a-1. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary. |
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Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-25, hsa-mir-29a, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-192, hsa-mir-129-1, hsa-mir-219a-1, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-132, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-129-2, hsa-mir-134, hsa-mir-29c, hsa-mir-219a-2, gga-mir-29a, gga-let-7a-3, gga-mir-92-1, gga-mir-124a, gga-let-7f, gga-let-7a-1, gga-mir-219a, gga-mir-124b, gga-let-7a-2, gga-mir-29c, gga-mir-9-2, hsa-mir-92b, gga-mir-9-1, gga-mir-124a-2, gga-mir-2954, tgu-mir-219b, tgu-mir-2954, tgu-mir-2976-1, tgu-mir-2976-3, tgu-mir-2976-2, tgu-mir-2985-1, tgu-mir-2985-2, tgu-mir-129, tgu-mir-9-2, tgu-mir-9-1, tgu-mir-124-1, tgu-mir-124-2, tgu-mir-124-3, tgu-let-7a-1, tgu-let-7a-3, tgu-let-7a-2, tgu-let-7a-4, tgu-let-7f, tgu-mir-29a-2, tgu-mir-219a, tgu-mir-92-1, tgu-mir-92-2, gga-mir-219b, tgu-mir-25, tgu-mir-192, tgu-mir-132, hsa-mir-219b, gga-mir-124c, gga-mir-9-3, gga-mir-92-2, gga-mir-9-4, gga-mir-2985-1, gga-mir-9b-1, gga-mir-132a, gga-mir-132b, gga-mir-132c, gga-mir-2985-2, gga-mir-129-1, gga-mir-129-2, gga-mir-129-3, gga-mir-9b-2
Probe sequences used for each target miRNA are given in Table 4. Table 4 Probes used for Taqman analysis of specific miRNA sequences miRBase name Company name Sequence detected tgu-let-7a let-7a 5'-UGAGGUAGUAGGUUGUAUAGUU-3' tgu-let-7f let-7f 5'-UGAGGUAGUAGAUUGUAUAGUU-3' tgu-miR-124 miR-124 5'-UAAGGCACGCGGUGAAUGCC-3' tgu-miR-9 miR-9 5'-UCUUUGGUUAUCUAGCUGUAUGA-3' tgu-miR-129-5p miR-129-5p 5'-CUUUUUGCGGUCUGGGCUUGC-3' tgu-miR-129-3p miR-129-3p 5'-AAGCCCUUACCCCAAAAAGCAU-3' tgu-miR-29a miR-29c 5'-UAGCACCAUUUGAAAUCGGU-3' tgu-miR-92 miR-92a 5'-UAUUGCACUUGUCCCGGCCUGU-3' tgu-miR-25 miR-25 5'-CAUUGCACUUGUCUCGGUCUGA-3' RNU6B RNU6B 5'-CGCAAGGAUGACACGCAAAUUCGUGAAGCGUUCCAUAUUUUU-3' tgu-miR-2954-5p novel51F-5p 5'-GCUGAGAGGGCUUGGGGAGAGGA-3' tgu-miR-2954-3p novel51F-3p 5'-CAUCCCCAUUCCACUCCUAGCA-3' (Northern validated) tgu-miR-2954R-5p novel51R-5p 5'-UGCUAGGAGUGGAAUGGGGAUG-3' tgu-miR-2954R-3p novel51R-3p 5'-UCCUCUCCCCAAGCCCUCUCAGC-3' Northern blotting to confirm novel miRNA tgu-miR-2954-3p was performed by modifying the protocol of [97].
[score:3]
Probe sequences used for each target miRNA are given in Table 4. Table 4 Probes used for Taqman analysis of specific miRNA sequences miRBase name Company name Sequence detected tgu-let-7a let-7a 5'-UGAGGUAGUAGGUUGUAUAGUU-3' tgu-let-7f let-7f 5'-UGAGGUAGUAGAUUGUAUAGUU-3' tgu-miR-124 miR-124 5'-UAAGGCACGCGGUGAAUGCC-3' tgu-miR-9 miR-9 5'-UCUUUGGUUAUCUAGCUGUAUGA-3' tgu-miR-129-5p miR-129-5p 5'-CUUUUUGCGGUCUGGGCUUGC-3' tgu-miR-129-3p miR-129-3p 5'-AAGCCCUUACCCCAAAAAGCAU-3' tgu-miR-29a miR-29c 5'-UAGCACCAUUUGAAAUCGGU-3' tgu-miR-92 miR-92a 5'-UAUUGCACUUGUCCCGGCCUGU-3' tgu-miR-25 miR-25 5'-CAUUGCACUUGUCUCGGUCUGA-3' RNU6B RNU6B 5'-CGCAAGGAUGACACGCAAAUUCGUGAAGCGUUCCAUAUUUUU-3' tgu-miR-2954-5p novel51F-5p 5'-GCUGAGAGGGCUUGGGGAGAGGA-3' tgu-miR-2954-3p novel51F-3p 5'-CAUCCCCAUUCCACUCCUAGCA-3' (Northern validated) tgu-miR-2954R-5p novel51R-5p 5'-UGCUAGGAGUGGAAUGGGGAUG-3' tgu-miR-2954R-3p novel51R-3p 5'-UCCUCUCCCCAAGCCCUCUCAGC-3' Northern blotting to confirm novel miRNA tgu-miR-2954-3p was performed by modifying the protocol of [97].
[score:3]
In nine out of ten cases, we observed the same direction of song response by TaqMan as in the small RNA-seq experiment, although the P-value by TaqMan was below 0.05 in only five cases (tgu-miR-124, tgu-miR-29a, tgu-miR-92, tgu-129-5p, and tgu-miR-2954-3p, Additional File 1, Table S4).
[score:2]
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