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8 publications mentioning sja-mir-2b

Open access articles that are associated with the species Schistosoma japonicum and mention the gene name mir-2b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 11
Interestingly, these five highly expressed miRNAs, miR-71b, miR-2a, miRNA-2e-5p, miRNA-2e-3p and miR-2f, were clustered in tandem at the same genomic locus (CNUS0000011792.1) (Figure 4A), whilst other miR-71 and miR-2 family members, miR-71a, miR-2d, miR-2b, and miR-2c, were clustered at another genomic locus (CNUS0000007682.1) in an inverted orientation (Figure 4B). [score:3]
In addition, both miR-71 and some miR-2 family members in tandem are found to be clustered in a reversal direction mo del on two genomic loci, and two pairs of novel S. japonicum miRNAs were derived from sense and antisense DNA strands at the same genomic loci. [score:2]
Although it is still not known how these Schistosoma miRNAs are evolutionarily related to each other, the observation that both the miR-71and miR-2 families lie within the same genomic clusters presents a mo del for the genesis of novel miRNAs, and implies that these genes exert their effects on worm development in a synergistic manner. [score:2]
Moreover, schistosomes also share some known miRNAs, such as bantam, miR-36, miR-60, miR-2 and miR-71 with Arthropoda and/or Nematoda animals. [score:1]
More interestingly, other miR-71 and miR-2 family members, miR-71a, miR-2d, miR-2b, and miR-2c, were clustered at another genomic locus (CNUS0000007682.1) in an inverted orientation. [score:1]
It should be pointed out that the cluster of miR-2 family members were found in silkworm (Bombyx mori), however, the combination of both miR-2 and miR-71 in the same clusters was not identified in other bilaterian animals. [score:1]
Here only two miRNA clusters identified by this study exhibited the similar genomic structure and components consisting of miR-71 and miR-2 family members. [score:1]
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2
[+] score: 8
The expression of sja-miR-2a and sja-miR-2b were found to be 31% and 26% of sja-miR-71a. [score:3]
Membranes were incubated with biotin-labeled probes complementary to candidate miRNA sequences (sja-miR-1a, sja-miR-1b, sja-miR-7, sja-miR-71b, sja-miR-71a, sja-miR-87, sja-miR-124, sja-miR-281, sja-miR-2b, sja-miR-2a, sja-miR-307, sja-miR-31, sja-miR-133, sja-miR-310, sja-miR-8, sja-miR-10, sja-miR-36, sja-miR-61, sja-miR-277, sja-miR-219, sja-miR-190, sja-miR-candidate-03). [score:1]
For instance, miR-1a and miR-1b had 584,105 and 552 read counts, respectively, while miR-2a and miR-2b had only 13,431 and 57 read counts, respectively. [score:1]
Cluster miR-71a contains 4 stem-loop structures encoding miR-71a, miR-2a, miR-2b, and a schistosome-specific miRNA (sja-miR-novel-12) that shares the seed sequence of the miR-13 family which has been extensively studied in other organisms. [score:1]
It also merit mention that the genomic arrangement of miRNAs, including miR-71a, the miR-2 family and sja-miR-novel-12 (miR-13), is conserved in S. mediterranea and S. mansoni. [score:1]
edu/) indicate that three conserved miRNA genes(sja-miR-71a, sja-miR-2a, sja-miR-2b) and one miRNA gene(sja-miR-novel-12), first reported here, are clustered together within approximately 350 base pair of each other. [score:1]
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3
[+] score: 7
In general, more miRNAs were expressed at stages other than the egg, except Sja-mir-2b and Sja-mir-71, which were constantly expressed during all stages with less than 10-fold variations (Fig. 5c). [score:5]
For instance, Sja-mir-2a, 2b and Sja-novel-36 were situated within one cluster (Fig. 4b, Table 1 and 2); however, the number of reads of Sja-mir-2a and Sja-novel-36 could be 100 times less than that of Sja-mir-2b. [score:1]
B. The predicted secondary structure of a pre-miRNA containing a cluster of Sja-mir-2a, Sja-mir-2b, and Sja-novel-36, which form three hairpin stem-loop structures. [score:1]
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4
[+] score: 7
1005423.g003 Fig 3Transfection of miR-2 (A), Let-7a (B), bantam (C), miR-8 (D and E), miR-31 (F), miR-1989 (G), and miR-3479 mimics (H) into HEK293T or Hela cells led to a significant reduction of luciferase activity from co -transfected plasmids containing their corresponding target regions. [score:3]
Transfection of miR-2 (A), Let-7a (B), bantam (C), miR-8 (D and E), miR-31 (F), miR-1989 (G), and miR-3479 mimics (H) into HEK293T or Hela cells led to a significant reduction of luciferase activity from co -transfected plasmids containing their corresponding target regions. [score:3]
Furthermore, co-transfection in these experiments with antisense miR -mimics (miR-2 and let-7a) led to de-repression of luciferase (Fig 3A and 3B). [score:1]
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5
[+] score: 4
Moreover, the four novel miRNAs transcribed principally in male adults, including one miRNA (sha-miR-new_46) encoded on the same scaffold as the mir-71/mir-2 cluster (KL250488.1) with a CPM more than 20-times higher than that in the library derived from adult female worms, suggest male-specific roles for these miRNAs in gene regulation. [score:2]
These results are consistent with reports of a mir-71/mir-2 cluster duplication in S. mansoni [10, 11] and S. japonicum [14, 34] on the female-sex (W) chromosome and on one autosome (chromosome 5), and further support roles in sex-specific traits, sexual differentiation, pairing of adult worms and reproductive processes in schistosomes. [score:1]
Notably, among the female-biased transcripts were the three miRNAs, sha-mir-71b, sha-mir-2b and sha-mir-2c (encoded in a cluster on scaffold KL251164.1), whereas the related miRNAs sha-mir-71a and sha-mir-2a (encoded in a cluster on scaffold KL250488.1) did not show sex-biased transcription. [score:1]
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6
[+] score: 4
Five out of the 13 known miRNAs coming from one family named as sja-miR-2, including family members from miR-2a to miR-2e. [score:1]
Blue: the largest family members of miR-2. Two conserved miRNAs named as bantam and let-7 were found in both S. japonicum and S. mansoni miRNAs. [score:1]
Blue: the largest family members of miR-2. Two conserved miRNAs named as bantam and let-7 were found in both S. japonicum and S. mansoni miRNAs. [score:1]
Besides the miR-2 family, another two miRNAs, miR-71 and miR-71b-5p, were also found from one family, which is located on two different scaffolds named as SJC_S000054 and SJC_S000102. [score:1]
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7
[+] score: 3
We observed 2 miRNAs (mir-2 and mir-71) expressed in both life cycle stages tested, 7 in adult worms only (mir-4, mir-6, mir-9, mir-32, mir-125, mir-3, mir-5) and 5 in schistosomula only (mir-20, mir-18, mir-22, mir-26, Bantam). [score:3]
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8
[+] score: 1
Furthermore, the 8 conserved miRNAs were identified as originating from 6 miRNA families, namely miR-124, miR-2 (miR-2b, miR-2e), bantam, miR-10, let-7 and miR-71 (miR-71, miR-71b). [score:1]
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