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4 publications mentioning mmu-mir-3963

Open access articles that are associated with the species Mus musculus and mention the gene name mir-3963. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 80
C2C12-DF cells (C2C12 cells grown in differentiation medium) was compared; 42 miRNAs were up-regulated with miR-206, miR-133b and miR-133a being commonly up-regulated (2.21, 1.68 and 1.63 fold in log2 ratio, respectively), and miR-487b, miR-6412 and miR-3963 were down-regulated (−1.98, −2.99 and −2.13 fold in log 2 ratio, respectively). [score:9]
We identified 161 miRNAs to be up-regulated, including miR-206, miR-133b and miR-133a (by 5.84, 5.46 and 5.37 fold in log2 ratio, respectively), while only miR-487b, miR-6412 and miR-3963 were down-regulated by about −1.00 in log2 ratio (−1.70, −0.96 and −0.93 fold in log2 ratio, respectively). [score:7]
miR-487b, miR-3963 and miR-6412 significantly suppress expression of troponin T. miRNA mimic transfection and MyoD and myogenin expression in C2C12 cell. [score:7]
We identified miR-206, miR-133a, and miR-133b as up-regulated miRNAs and miR-487b, miR-3963 and miR-6412 as down-regulated miRNAs in differentiating cells. [score:7]
We found that miR-206, miR-133a, miR-133b were up-regulated, and miR-487b, miR-3963, and miR-6412 were down-regulated during myogenic differentiation. [score:7]
The suppression of troponin T expression was observed from day 1 for miR-487b and miR-6412, and from day 2 for miR-3963. [score:5]
Figure 3 miR-487b, miR-3963 and miR-6412 transfection down-regulated slow type MyHC. [score:4]
Figure 2 miR-487b and miR-3963 transfection down-regulated fast type MyHC. [score:4]
Therefore, we searched for specific miRNAs that are involved in myogenic differentiation, and our miRNA microarray data demonstrated two novel miRNAs, miR-487b, miR-3963 and miR-6412 that are specifically down-regulated in C2C12 cells cultured in differentiation medium. [score:4]
However, we were unable to identify candidate targets associated with muscle differentiation for miR-3963 and miR-6412. [score:3]
The expression of miR-487b, miR-3963 and miR-6412 was assessed by RT-qPCR. [score:3]
Transfection of miR-1 positive control, miR-206 and miR-133a resulted in a significant increase of the troponin T -positive cell ratio, while transfection of miR-487b, miR-3963 and miR-6412 mimics significantly decreased troponin T expression (Figure  1). [score:3]
RT-qPCR forWe performed quantitative PCR analysis to assess expression of miR-487b, miR-3963 and miR-6412. [score:3]
We performed quantitative PCR analysis to assess expression of miR-487b, miR-3963 and miR-6412. [score:3]
Our results suggest that miR-487b, miR-3963 and miR-6412 are novel muscle-specific miRNAs that may function as negative regulators of skeletal muscle differentiation. [score:2]
Microrna (miRNA) C2C12 myoblast-derived cell Skeletal muscle Myogenic differentiation miR-487b miR-3963 miR-6412 Skeletal muscle development is complex, involving several signaling pathways. [score:2]
In this study, we identified miR-486b, miR-3963 and miR-6412 as novel myogenic differentiation-related miRNAs, the functions of which are largely unknown. [score:1]
In the present study, we identified miR-487b, miR-3963 and miR-6412 as new skeletal muscle differentiation -associated miRNAs. [score:1]
Transfection of miR-487b and miR-3963 mimics resulted in a significant decrease in the MyHC (fast) positive ratio (Figure  2), while miR-1 positive control, miR-206, miR-133a, miR-133b and miR-6412 did not affect the MyHC (fast) positive cell ratio. [score:1]
Therefore, we focused on miR-206, miR-133a, miR-133b, miR-487b, miR-6412 and miR-3963 as a potential myogenic differentiation-related miRNAs, and the effect of transfection of these miRNAs on cell differentiation was assessed. [score:1]
Moreover, no reports have been published on the function of miR-3963 or miR-6412. [score:1]
Next, we focused on miR-487b, miR-3963 and miR-6412 transfection, which resulted in decreased differentiation. [score:1]
miR-487b, miR-3963 and miR-6412 mimics significantly decreased the MyHC (slow) positive cell ratio (Figure  3). [score:1]
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2
[+] score: 16
For example, miR-877-5p and miR-5114 were up-regulated, whereas miR-3963, miR-378a-3p, miR-193b-3p, miR-125a-5p, miR-378b, miR-365-3p, let-7e-5p, and miR-712-5p were down-regulated in the 0.01 Gy-irradiated mouse spleens compared with the sham spleens. [score:6]
Consistent with the results of the microarray analysis, the expression levels of miR-378a-3p, miR-193b-3p, miR-125a-5p, and miR-712-5p decreased in the 0.01 Gy-irradiated mouse spleens compared with the sham group, whereas the expression of miR-3963 did not change significantly. [score:4]
Down-regulation of miR-125a-5p and miR-3963 was observed only in the kidney (Fig. 1C). [score:4]
In our qRT-PCR analysis (Fig. 1A), only 5 miRNAs (miR-378a-3p, miR-193b-3p, miR-125a-5p, miR-712-5p, and miR-3963) generated acceptable Ct values, whereas the other 5 miRNAs (miR-877-5p, miR-5114, miR-378b, miR-365-3p, and let-7e-5p) fell below the minimum threshold because of their lower abundance. [score:1]
The levels of miR-378a-3p, miR-193b-3p, miR-125a-5p, and miR-712-5p decreased in response to LDIR, although the level of miR-3963 did not (Fig. 1B). [score:1]
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3
[+] score: 5
The most abundant expressions show the miRNAs miR-122-5p, miR-6366, miR-3963 and miR-5100, while relatively low expression is observed for the miRNAs miR-1196-5p, miR-468-3p and miR-669n. [score:5]
[1 to 20 of 1 sentences]
4
[+] score: 3
Two of the most highly expressed miRs are only recently described: mmu-miR-3963 (on chromosome 3) [32], and mmu-miR-5100 (on chromosome 11) 33– 36. [score:3]
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