9 publications mentioning mdm-MIR171l

Open access articles that are associated with the species Malus domestica and mention the gene name MIR171l. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

The potential target of miRNA171, MdHAM, fluctuated in its expression level from 45 to 105 DABB, and was then downregulated after 125 DABB.

In the mo del, low expression of miRNA164, miRNA171, and high expression of miRNA166 and their potential target genes affected SAM development and reduced the duration of shoot growth in YF trees.

The expression of miRNA171 in CF trees was upregulated on the 8th day after the application of the.

Based on the GO annotation of the target genes and with the KEGG pathway analysis, the potential targets of miR164, miR166, miR171, miR172, and miR482 are involved in meristem development (Table 3).

Our results indicate that miRNA164, miRNA166, and miRNA171 are potentially involved in SAM development and differentially expressed in YF vs.

In contrast, five members of mdm-miR164, three members of mdm-miR171, six members of mdm-miR172, three members of mdm-miR393, nine members of mdm-miR395, two members of mdm-miR396, six members of mdm-miR399, two members of mdm-miR5225, two members of mdm-miR7124, and mdm-miR858 were all downregulated in YF shoot tips.

The potential targets of miR159, miR166, miR167, miR171, miR172, miR393, miR858, and miR828 are involved in cell growth.

The expression of miRNA171 in YF trees exhibited little response to the.

The expression of miRNA171 in YF was significantly lower than CF from 65 to 145 DABB.

miRNA171 potentially targets the HAM subfamily genes, SCL6-II, SCL6-III, and SCL6-IV, within the GRAS gene family.

The expression of miRNA171 in CF and YF increased from 45 to 65, decreased from 85 to 125, and then increased to a high level at 145 DABB.

The expression level of miRNA171 was significantly lower in YF trees than in CF trees.

miRNA171 affects the maintenance of SAM indeterminacy by regulating the shoot apical meristem WUS-CLV feedback loop (Schulze et al., 2010).

The top five miRNA families, mdm-miR156, mdm-miR172, mdm-miR171, mdm-miR167, and mdm-miR399, had more than 10 members.

2

Several miRNAs were differentially expressed across the stem tissues: miR160 showed higher expression in the phloem than xylem and was barely detectable in periderm; miR394 showed higher expression in the xylem than phloem and was barely detectable in periderm; miR168 was more abundant in periderm than phloem and xylem; miR171 was absent from phloem but was detected both in periderm and xylem.

Conservation status miRNA family Arabidopsis Oryza(rice) Populus(poplar) Predicted target gene(s) miR156 √ √ √Squamosa promoter -binding proteins[57] miR159/319 √ √ √GAMYB transcription factors[57] miR160 √ √ √Auxin response factors (ARF) [57] miR162 √ √ √DICER-LIKE 1 (DCL1) [57] miR164 √ √ √NAC domain transcription factors[57] miR156/166 √ √ √HD-ZIP transcription factors[57] miR167 √ √ √Auxin response factors (ARF) [57] miR168 √ √ √ARGONAUTE 1 (AGO1) [57] miR169 √ √ √HAP2-like transcription factors[57] miR171 √ √ √Scarecrow-like transcription factors[57] miR172 √ √ √APETALA 2 transcription factors[58] miR390 √ √ √TAS3[59] miR393 √ √ √F-box transcription factors (TIR1) [60] miR394 √ √ √F-box transcription factors[60] miR396 √ √ √GRF, rhodenase[60] miR397 √ √ √laccase[60] miR398 √ √ √Copper superoxid dismutase, CytC oxidase[60] miR403 √ √ √ARGONAUTE 2 (AGO2)[20] miR408 √ √Peptide chain release factor, laccase[20] miR475 √PPR proteins[8] miR476 √PPR proteins[8] Figure 1 Differential expression of miRNAs in apple tissues.

Conservation status miRNA family Arabidopsis Oryza(rice) Populus(poplar) Predicted target gene(s) miR156 √ √ √Squamosa promoter -binding proteins[57] miR159/319 √ √ √GAMYB transcription factors[57] miR160 √ √ √Auxin response factors (ARF) [57] miR162 √ √ √DICER-LIKE 1 (DCL1) [57] miR164 √ √ √NAC domain transcription factors[57] miR156/166 √ √ √HD-ZIP transcription factors[57] miR167 √ √ √Auxin response factors (ARF) [57] miR168 √ √ √ARGONAUTE 1 (AGO1) [57] miR169 √ √ √HAP2-like transcription factors[57] miR171 √ √ √Scarecrow-like transcription factors[57] miR172 √ √ √APETALA 2 transcription factors[58] miR390 √ √ √TAS3[59] miR393 √ √ √F-box transcription factors (TIR1) [60] miR394 √ √ √F-box transcription factors[60] miR396 √ √ √GRF, rhodenase[60] miR397 √ √ √laccase[60] miR398 √ √ √Copper superoxid dismutase, CytC oxidase[60] miR403 √ √ √ARGONAUTE 2 (AGO2)[20] miR408 √ √Peptide chain release factor, laccase[20] miR475 √PPR proteins[8] miR476 √PPR proteins[8] Figure 1 Differential expression of miRNAs in apple tissues.

D-F, Spatial expression of miR156, miR167 and miR171 in Arabidopsis inflorescence stem; miR156 and miR167, but not miR171, were detected in Arabidopsis phloem by in situ hybridization using appropriate antisense oligonucleotide probes.

C, Stem-loop RT-PCR analyses using 10 ng total RNA of miR160, miR162, miR164, miR168, miR169, miR171, miR390, miR393, miR394, miR396, miR397, miR398, miR403, miR408, miR475, and miR476 expression.

A-C, Spatial expression of miR156, miR167 and miR171 in apple seedling stem; miR156 and miR167, but not miR171, were detected in apple vascular tissue by in situ hybridization using appropriate antisense oligonucleotide probes.

In contrast, miR171, miR394 and miR403, which were absent from apple phloem sap, were detected in pumpkin phloem sap at a lower level than in the pumpkin vascular bundle.

Antisense oligonucleotides corresponding to miR156 and miR167 but not miR171 produced a hybridization signal in the phloem of apple seedling stems (Figure 2A-C).

Similarly, miR171, miR394 and miR403, which were not detected in apple phloem sap, were also less abundant in the pumpkin phloem sap sample and, with the exception of miR171, were not cloned from Brassica napus phloem sap [37], implying conservation of cell-autonomy for these miRNAs.

Using this approach miR156, miR159, miR160, miR162, miR167, miR169, miR396 and miR398 were clearly detectable; miR172, miR390 and miR393 produced a weak amplification signal; miR166 and miR397 amplification did not produce the expected product, but resulted in a smear not detected in the minus-RT control; miR164, miR168, miR171, miR394, miR403, miR408 and the miRNAs specific to poplar (miR475 and miR476) were not detected (Figure 4).

Both miR156 and miR167 antisense probes produced a strong hybridization signal in the phloem (Figure 2D-E), while no signal was detected with miR171 antisense oligonucleotide probe (Figure 2F), consistent with the RT-PCR results.

Of eighteen detected in at least one of the analysed apple tissues, all except miR171 were detected in the phloem tissue.

3

Arabidopsis miR171 -targeted scarecrow-like proteins bind to GT cis-elements and mediate gibberellin-regulated chlorophyll biosynthesis under light conditions.

Among them, miR164, miR171, miR482, and miR5225 and several reported novel miRNAs enriched in floral bud and also expressed stronger in adult materials in the previous studies (Xia et al., 2012; Xing et al., 2014), indicating their conserved roles in apple flowering process.

Here, the floral bud-enriched miR482 and miR2118 are involved in plant fungal pathogen resistance (Zhai et al., 2011); miR171 play roles in ABA and GA pathways during flower development (Ma Z. et al., 2014).

Many DE-miRNAs in our results were reported to be stress responsive, such as the floral bud-enriched ones including miR159, miR171, miR319, miR396, miR399, miR482, miR1511, and miR2118 (Table 2).

Among these families, major malus miRNAs, including miRNA156 (9 members), miRNA171 (14), miRNA172 (14), miRNA167 (10), and miRNA395 (9), were detected (Figure 2A).

4

The first group of miRNAs to be identified in plants included miR171, which accumulates predominantly in the inflorescence tissues of Arabidopsis thaliana and targets several members of the SCARECROW-LIKE (SCL) family of transcription factors, which is involved in gibberellin-regulated chlorophyll biosynthesis (Llave et al., 2002).

Also, Md-miR171 had the greatest difference in expression following ALT1 inoculation.

A few studies have reported the presence of conserved abiotic- and biotic-stress -induced miRNAs in apple, such as miR164, miR159, and miR171, suggesting that sRNAs also play an important role in defense in this plant (Zhang et al., 2011).

5

However, target genes for miR167 were detected only in predicted gene mo dels, and miR171 had predicted target genes only in mesocarp cDNA sequences.

6

For example, we found that miR160, miR156, miR171, miR172, miR395, and miR398 were differentially expressed upon debagging, these families are predicted to be regulated by UV-B radiation in Arabidopsis and juvenile maize leaves, respectively (Zhou et al., 2007).

The largest miRNA family, miR156, had 29 members, followed by miR171 and miR172 with both 15 members (Figure 3).

7

SCL21 has been recently described in barley as regulated by miRNA171 and interacting with Phytochrome PAT1 [70].

8

Five of the miRNAs (miR171, miR166i, miR159e, miR845, and miR396e) were enriched in the white flowers of Maroussia.

9

A majority of the 42 known miRNA families had several members, and five families, mdm-miR156, mdm-miR171, mdm-miR172, mdm-miR167 and mdm-miR399, had 31, 15, 15, 10 and 10 members, respectively.