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5 publications mentioning mes-MIR482

Open access articles that are associated with the species Manihot esculenta and mention the gene name MIR482. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 31
Other miRNAs from this paper: mes-MIR159a, mes-MIR167a, mes-MIR171a, mes-MIR156a, mes-MIR156b, mes-MIR156c, mes-MIR156d, mes-MIR156e, mes-MIR156f, mes-MIR156g, mes-MIR156h, mes-MIR156i, mes-MIR156j, mes-MIR156k, mes-MIR160a, mes-MIR160b, mes-MIR160c, mes-MIR160d, mes-MIR160e, mes-MIR160f, mes-MIR160g, mes-MIR160h, mes-MIR166a, mes-MIR166b, mes-MIR166c, mes-MIR166d, mes-MIR166e, mes-MIR166f, mes-MIR166g, mes-MIR166h, mes-MIR166i, mes-MIR166j, mes-MIR167b, mes-MIR167c, mes-MIR167d, mes-MIR167e, mes-MIR167f, mes-MIR167g, mes-MIR167h, mes-MIR169a, mes-MIR169b, mes-MIR169c, mes-MIR169d, mes-MIR169e, mes-MIR169f, mes-MIR169g, mes-MIR169h, mes-MIR169i, mes-MIR169j, mes-MIR169k, mes-MIR169l, mes-MIR169m, mes-MIR169n, mes-MIR169o, mes-MIR169p, mes-MIR169q, mes-MIR169r, mes-MIR169s, mes-MIR169t, mes-MIR169u, mes-MIR169v, mes-MIR169w, mes-MIR169x, mes-MIR169y, mes-MIR169z, mes-MIR169aa, mes-MIR169ab, mes-MIR169ac, mes-MIR171b, mes-MIR171c, mes-MIR171d, mes-MIR171e, mes-MIR171f, mes-MIR171g, mes-MIR171h, mes-MIR171i, mes-MIR171j, mes-MIR171k, mes-MIR319f, mes-MIR390, mes-MIR393a, mes-MIR393b, mes-MIR393c, mes-MIR393d, mes-MIR395a, mes-MIR395b, mes-MIR395c, mes-MIR395d, mes-MIR395e, mes-MIR477h, mes-MIR477i, mes-MIR477a, mes-MIR477b, mes-MIR477c, mes-MIR477d, mes-MIR477e, mes-MIR477f, mes-MIR477g, mes-MIR530b, mes-MIR535a, mes-MIR535b, mes-MIR828a, mes-MIR828b, mes-MIR171l, mes-MIR390b, mes-MIR397b, mes-MIR398, mes-MIR399h, mes-MIR477j, mes-MIR477k, mes-MIR2118, mes-MIR482b, mes-MIR482d, mes-MIR535c, mes-MIR535d, mes-MIR1446b, mes-MIR6445, mes-MIR9386, mes-MIR169ad, mes-MIR11891, mes-MIR11892
Of the cleaved transcripts, three encoding NB -LRR disease resistance proteins (cassava4.1_031767, cassava4.1_001198 and cassava4.1_033128) and one encoding a PPR protein (cassava4.1_002995) were confirmed to be targets of members of the miR482 and miR2118 superfamily. [score:5]
Total RNA from cassava leaves was subjected to 5′ RACE analysis to validate cleavage of selected predicted targets of members of the miR482/2118 superfamily. [score:3]
The number of PHAS loci in each class is indicated after the nameOur analysis shows that similar to results in other plant species, members of the miR482/2118 superfamily in cassava trigger the production of phasiRNAs from NB-LRR disease resistance genes (Fig.   7). [score:3]
As expected, miRNAs from the same family, including miR482 and miR535 families, showed similar expression levels across tissues. [score:3]
Correspondingly, very low levels of miR482, miR477, and miR535 families were observed in callus tissues while mes-miR530b, mes-miR535c, mes-miR169ad, and mes-miR399h appeared not to be expressed in leaves. [score:3]
The number of PHAS loci in each class is indicated after the name Our analysis shows that similar to results in other plant species, members of the miR482/2118 superfamily in cassava trigger the production of phasiRNAs from NB-LRR disease resistance genes (Fig.   7). [score:3]
Although phasiRNAs from miR482/2118 PHAS targets generally tended to be uniformly distributed across tissues as exemplified by loci found at chr6914:328331.. [score:3]
To confirm predictions that mes-miR2118 and mes-miR482 cleave their target NB-LRR genes in cassava, we carried out a modified RLM-5′ RACE analysis, using total RNAs extracted from cassava leaves. [score:3]
miR482/2118 var. [score:1]
Thus, miRNAs found to occur at high levels, notably members of the miR482 and miR2118 families, also showed high abundance in qRT-PCR results. [score:1]
are variants of miR482/2118 superfamily Abundance levels were observed to vary considerably across tissues, except for mes-miR11892, and to a lesser extent, mes-miR169ad, which more or less occurred at similar levels in most of the tissues analyzed. [score:1]
Some of the miRNAs showed very high abundance in male flower tissues, notably miR482 and miR477 families, mes-miR2118, and mes-miR1446b. [score:1]
are variants of miR482/2118 superfamily Abundance levels were observed to vary considerably across tissues, except for mes-miR11892, and to a lesser extent, mes-miR169ad, which more or less occurred at similar levels in most of the tissues analyzed. [score:1]
[1 to 20 of 13 sentences]
2
[+] score: 15
Other miRNAs from this paper: rco-MIR156a, rco-MIR156b, rco-MIR156c, rco-MIR156d, rco-MIR156e, rco-MIR156f, rco-MIR156g, rco-MIR156h, rco-MIR159, rco-MIR160a, rco-MIR160b, rco-MIR162, rco-MIR164a, rco-MIR164b, rco-MIR164c, rco-MIR164d, rco-MIR166a, rco-MIR166b, rco-MIR166c, rco-MIR166d, rco-MIR166e, rco-MIR167a, rco-MIR167b, rco-MIR167c, rco-MIR168, rco-MIR169a, rco-MIR169b, rco-MIR169c, rco-MIR171a, rco-MIR171b, rco-MIR171c, rco-MIR171d, rco-MIR171e, rco-MIR171f, rco-MIR171g, rco-MIR172, rco-MIR319a, rco-MIR319b, rco-MIR319c, rco-MIR319d, rco-MIR390a, rco-MIR390b, rco-MIR393, rco-MIR395a, rco-MIR395b, rco-MIR395c, rco-MIR395d, rco-MIR395e, rco-MIR396, rco-MIR398a, rco-MIR398b, rco-MIR399a, rco-MIR399b, rco-MIR399c, rco-MIR399d, rco-MIR399e, rco-MIR399f, rco-MIR408, rco-MIR160c, mes-MIR159a, mes-MIR167a, mes-MIR168a, mes-MIR171a, mes-MIR172a, mes-MIR399a, mes-MIR408, mes-MIR156a, mes-MIR156b, mes-MIR156c, mes-MIR156d, mes-MIR156e, mes-MIR156f, mes-MIR156g, mes-MIR156h, mes-MIR156i, mes-MIR156j, mes-MIR156k, mes-MIR159b, mes-MIR159c, mes-MIR159d, mes-MIR160a, mes-MIR160b, mes-MIR160c, mes-MIR160d, mes-MIR160e, mes-MIR160f, mes-MIR160g, mes-MIR160h, mes-MIR162, mes-MIR164a, mes-MIR164b, mes-MIR164c, mes-MIR164d, mes-MIR166a, mes-MIR166b, mes-MIR166c, mes-MIR166d, mes-MIR166e, mes-MIR166f, mes-MIR166g, mes-MIR166h, mes-MIR166i, mes-MIR166j, mes-MIR167b, mes-MIR167c, mes-MIR167d, mes-MIR167e, mes-MIR167f, mes-MIR167g, mes-MIR167h, mes-MIR169a, mes-MIR169b, mes-MIR169c, mes-MIR169d, mes-MIR169e, mes-MIR169f, mes-MIR169g, mes-MIR169h, mes-MIR169i, mes-MIR169j, mes-MIR169k, mes-MIR169l, mes-MIR169m, mes-MIR169n, mes-MIR169o, mes-MIR169p, mes-MIR169q, mes-MIR169r, mes-MIR169s, mes-MIR169t, mes-MIR169u, mes-MIR169v, mes-MIR169w, mes-MIR169x, mes-MIR169y, mes-MIR169z, mes-MIR169aa, mes-MIR169ab, mes-MIR169ac, mes-MIR171b, mes-MIR171c, mes-MIR171d, mes-MIR171e, mes-MIR171f, mes-MIR171g, mes-MIR171h, mes-MIR171i, mes-MIR171j, mes-MIR171k, mes-MIR172b, mes-MIR172c, mes-MIR172d, mes-MIR172e, mes-MIR172f, mes-MIR319a, mes-MIR319b, mes-MIR319c, mes-MIR319d, mes-MIR319e, mes-MIR319f, mes-MIR319g, mes-MIR319h, mes-MIR390, mes-MIR393a, mes-MIR393b, mes-MIR393c, mes-MIR393d, mes-MIR395a, mes-MIR395b, mes-MIR395c, mes-MIR395d, mes-MIR395e, mes-MIR396a, mes-MIR396b, mes-MIR396c, mes-MIR396d, mes-MIR396e, mes-MIR396f, mes-MIR399b, mes-MIR399c, mes-MIR399d, mes-MIR399e, mes-MIR399f, mes-MIR399g, mes-MIR477h, mes-MIR477i, mes-MIR477a, mes-MIR477b, mes-MIR477c, mes-MIR477d, mes-MIR477e, mes-MIR477f, mes-MIR477g, mes-MIR828a, mes-MIR828b, mes-MIR171l, mes-MIR398, mes-MIR399h, mes-MIR477j, mes-MIR477k, mes-MIR2118, mes-MIR3627, mes-MIR169ad
Alternatively, it is plausible that miR482 did not regulate NBS-LRR genes in Euphorbiaceous species, which is consistent with the prediction that few NBS-LRR genes were targeted by miR482 in Cassava or castor bean, respectively. [score:4]
For example, sly-miR482 and sly-miR2118 in tomato and ath-miR472 and ath-miR825 in Arabidopsis all target a large number of NBS-LRR genes. [score:3]
However, miR482 in Cassava was rarely sequenced in the current data sets (Additional file 5), indicating that miR482 might not be expressed in the tissues we examined. [score:3]
This is in contrast to the results in Medicago and tomatoes, where a large number of NBS-LRR genes have been reported to be targeted by one or two miRNA initiators, such as miR482 [18, 19]. [score:3]
ta-siRNAs may also arise from coding genes; a large number of genes encoding nucleotide binding site–leucine-rich repeat (NBS-LRR) plant innate immune receptors have been reported to give rise to ta-siRNAs, which were triggered by miR482 and miR2118 [16, 18, 19]. [score:1]
We were able to identify one locus encoding miR482 in Cassava genome (Additional files 5). [score:1]
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3
[+] score: 11
miRNA downregulation showed in this work also agrees with the general mo del, since three repressed families (miR535, miR395 and miR482) were predicted to target NB-LRR and LRR resistance-like genes in cassava. [score:6]
These repressed families included miR535, miR395 and miR482 which were predicted to target various candidate NB-LRR and LRR disease resistance proteins. [score:5]
[1 to 20 of 2 sentences]
4
[+] score: 8
Other miRNAs from this paper: mes-MIR159a, mes-MIR167a, mes-MIR168a, mes-MIR171a, mes-MIR172a, mes-MIR399a, mes-MIR408, mes-MIR156a, mes-MIR156b, mes-MIR156c, mes-MIR156d, mes-MIR156e, mes-MIR156f, mes-MIR156g, mes-MIR156h, mes-MIR156i, mes-MIR156j, mes-MIR156k, mes-MIR159b, mes-MIR159c, mes-MIR159d, mes-MIR160a, mes-MIR160b, mes-MIR160c, mes-MIR160d, mes-MIR160e, mes-MIR160f, mes-MIR160g, mes-MIR160h, mes-MIR162, mes-MIR164a, mes-MIR164b, mes-MIR164c, mes-MIR164d, mes-MIR166a, mes-MIR166b, mes-MIR166c, mes-MIR166d, mes-MIR166e, mes-MIR166f, mes-MIR166g, mes-MIR166h, mes-MIR166i, mes-MIR166j, mes-MIR167b, mes-MIR167c, mes-MIR167d, mes-MIR167e, mes-MIR167f, mes-MIR167g, mes-MIR167h, mes-MIR169a, mes-MIR169b, mes-MIR169c, mes-MIR169d, mes-MIR169e, mes-MIR169f, mes-MIR169g, mes-MIR169h, mes-MIR169i, mes-MIR169j, mes-MIR169k, mes-MIR169l, mes-MIR169m, mes-MIR169n, mes-MIR169o, mes-MIR169p, mes-MIR169q, mes-MIR169r, mes-MIR169s, mes-MIR169t, mes-MIR169u, mes-MIR169v, mes-MIR169w, mes-MIR169x, mes-MIR169y, mes-MIR169z, mes-MIR169aa, mes-MIR169ab, mes-MIR169ac, mes-MIR171b, mes-MIR171c, mes-MIR171d, mes-MIR171e, mes-MIR171f, mes-MIR171g, mes-MIR171h, mes-MIR171i, mes-MIR171j, mes-MIR171k, mes-MIR172b, mes-MIR172c, mes-MIR172d, mes-MIR172e, mes-MIR172f, mes-MIR319a, mes-MIR319b, mes-MIR319c, mes-MIR319d, mes-MIR319e, mes-MIR319f, mes-MIR319g, mes-MIR319h, mes-MIR390, mes-MIR393a, mes-MIR393b, mes-MIR393c, mes-MIR393d, mes-MIR395a, mes-MIR395b, mes-MIR395c, mes-MIR395d, mes-MIR395e, mes-MIR396a, mes-MIR396b, mes-MIR396c, mes-MIR396d, mes-MIR396e, mes-MIR396f, mes-MIR397, mes-MIR399b, mes-MIR399c, mes-MIR399d, mes-MIR399e, mes-MIR399f, mes-MIR399g, mes-MIR535a, mes-MIR535b, mes-MIR827, mes-MIR2275, mes-MIR171l, mes-MIR398, mes-MIR399h, mes-MIR2118, mes-MIR535c, mes-MIR535d, mes-MIR169ad
For example in tomato, sequence diverse members of the miR482 family target large numbers of NB-LRR mRNAs, which in turn produce phased tasiRNAs [97], while in Medicago truncatula miR2118 causes large amounts of phased secondary tasiRNAs from NB-LRR mRNAs [98]. [score:3]
The four families, miR319, miR396, miR482, and miR535 were found to contain 29, 26, 21, and 20 families, respectively. [score:1]
All the miRNAs were experimentally validated except for miR482/miR2118 (Fig 5A). [score:1]
A member of the miR482 family identified in this study was also found to be 22 nt. [score:1]
Seven identified conserved miRNAs: miR169, miR170/171, miR408, miR476 and miR482/2118 were selected for RT-PCR validation studies. [score:1]
Studies demonstrate that members of the miR482/2118 superfamily initiate large numbers of phased, secondary tasiRNA accumulation from plant NB-LRR class of resistance genes. [score:1]
[1 to 20 of 6 sentences]
5
[+] score: 3
AV1, AV2 and IR were targeted by 3 (miR160f*, miR1082b, miR1446a), 2 (miR399c*, miR2927) and 4 (miR478a, miR482, miR2119, miR3633b) miR/miR* sequences, respectively (Table S8 in File S1). [score:3]
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