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3 publications mentioning bdi-MIR2118a

Open access articles that are associated with the species Brachypodium distachyon and mention the gene name MIR2118a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

[+] score: 39
Other miRNAs from this paper: osa-MIR160a, osa-MIR160b, osa-MIR160c, osa-MIR160d, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR169a, osa-MIR395b, osa-MIR395d, osa-MIR395e, osa-MIR395g, osa-MIR395h, osa-MIR395i, osa-MIR395j, osa-MIR395k, osa-MIR395l, osa-MIR395s, osa-MIR395t, osa-MIR395c, osa-MIR395a, osa-MIR395f, osa-MIR395u, osa-MIR398a, osa-MIR398b, osa-MIR160e, osa-MIR160f, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR169b, osa-MIR169c, osa-MIR169d, osa-MIR169e, osa-MIR169f, osa-MIR169g, osa-MIR169h, osa-MIR169i, osa-MIR169j, osa-MIR169k, osa-MIR169l, osa-MIR169m, osa-MIR169n, osa-MIR169o, osa-MIR169p, osa-MIR169q, osa-MIR167j, osa-MIR437, osa-MIR395m, osa-MIR395n, osa-MIR395o, osa-MIR395p, osa-MIR395q, osa-MIR395v, osa-MIR395w, osa-MIR395r, osa-MIR818a, osa-MIR818b, osa-MIR818c, osa-MIR818d, osa-MIR818e, tae-MIR160, tae-MIR167a, tae-MIR1117, tae-MIR1118, tae-MIR1120a, tae-MIR1122a, tae-MIR1125, tae-MIR1127a, tae-MIR1128, tae-MIR1131, tae-MIR1133, tae-MIR1135, tae-MIR1136, tae-MIR1139, osa-MIR169r, osa-MIR1436, osa-MIR1439, osa-MIR2118a, osa-MIR2118b, osa-MIR2118c, osa-MIR2118d, osa-MIR2118e, osa-MIR2118f, osa-MIR2118g, osa-MIR2118h, osa-MIR2118i, osa-MIR2118j, osa-MIR2118k, osa-MIR2118l, osa-MIR2118m, osa-MIR2118n, osa-MIR2118o, osa-MIR2118p, osa-MIR2118q, osa-MIR2118r, bdi-MIR167a, bdi-MIR1139, bdi-MIR1122, bdi-MIR437, bdi-MIR169b, bdi-MIR1127, bdi-MIR1135, osa-MIR395x, osa-MIR395y, tae-MIR167b, tae-MIR169, tae-MIR395a, tae-MIR395b, tae-MIR398, tae-MIR5085, bdi-MIR5070, bdi-MIR169d, bdi-MIR169i, bdi-MIR395a, bdi-MIR169j, bdi-MIR160a, bdi-MIR395b, bdi-MIR167b, bdi-MIR160b, bdi-MIR167c, bdi-MIR169k, bdi-MIR160c, bdi-MIR167d, bdi-MIR169g, bdi-MIR160d, bdi-MIR160e, bdi-MIR169e, bdi-MIR398a, bdi-MIR169a, bdi-MIR169h, bdi-MIR169c, bdi-MIR395c, bdi-MIR5180b, bdi-MIR5175a, bdi-MIR5175b, bdi-MIR395d, bdi-MIR398b, bdi-MIR5180a, bdi-MIR169f, bdi-MIR395m, bdi-MIR395e, bdi-MIR395f, bdi-MIR395g, bdi-MIR395h, bdi-MIR395j, bdi-MIR395k, bdi-MIR395l, bdi-MIR395n, osa-MIR818f, bdi-MIR167e, bdi-MIR395o, bdi-MIR395p, bdi-MIR5049, bdi-MIR160f, bdi-MIR167f, bdi-MIR167g, bdi-MIR169l, bdi-MIR169m, bdi-MIR169n, bdi-MIR395q, bdi-MIR2118b, tae-MIR1122b, tae-MIR1127b, tae-MIR1122c, tae-MIR167c, tae-MIR5175, tae-MIR1120b, tae-MIR1120c, tae-MIR6197, tae-MIR5049
Other miRNAs included in this study (miR169, miR5085, miR6220, miR2118) may also be expressed, under stress conditions, in other wheat tissues and/or at different developmental stages. [score:4]
Expression of pre-miR2118 in Triticum aestivum L. cv Chinese Spring (CS) (A) Endpoint PCR results (B) qRT-PCR results. [score:3]
Experimental evidence for expression of pre-miR2118 from wheat 5D chromosome. [score:3]
In order to show expression of selected pre-miRNAs (pre-miR2118, pre-miR169, pre-miR5085, pre-miR6220, pre-miR5070), RT-PCR and qRT-PCR was performed using Chinese Spring cDNA. [score:3]
0069801.g005 Figure 5Expression of pre-miR2118 in Triticum aestivum L. cv Chinese Spring (CS) (A) Endpoint PCR results (B) qRT-PCR results. [score:3]
Expression of pre-miR2118 in adult leaves of wheat, grown under standard greenhouse conditions was shown. [score:3]
Evidence for pre-miR2118 expression in wheat. [score:3]
5D-specific pre-miR2118 was shown to be expressed from the leaf tissue of CS grown under standard greenhouse conditions (Figure 5). [score:3]
Among these, expression of miR2118 was experimentally shown. [score:3]
pre-miR169, pre-miR5085 and pre-miR2118 coding regions were found to be 5D chromosome-specific. [score:1]
To experimentally validate 5D chromosome localization of selected pre-miRNAs (miR169, miR5085, miR2118, miR5070, miR6220), PCR screening was carried out using DNA from flow-sorted 5D chromosome arms. [score:1]
Three of these pre-miRNAs (miR169, miR5085, miR2118) were shown to be 5D specific (Figure 2). [score:1]
0069801.g002 Figure 2PCR screening of pre-miRNA coding sequences in flow sorted 5D short and long chromosome arms (5DS and 5DL); Triticum aestivum L. cv Chinese Spring (CS) and nullitetrasomic lines (N5D-T5A and N5D-T5B) (A) pre-miR169 (B) pre-miR5085 (C) pre miR5070 (D) pre-miR6220 (E) pre-miR2118. [score:1]
PCR screening of pre-miRNA coding sequences in flow sorted 5D short and long chromosome arms (5DS and 5DL); Triticum aestivum L. cv Chinese Spring (CS) and nullitetrasomic lines (N5D-T5A and N5D-T5B) (A) pre-miR169 (B) pre-miR5085 (C) pre miR5070 (D) pre-miR6220 (E) pre-miR2118. [score:1]
2.5 mM MgCl [2] (stock concentration : 25 mM) was used for the amplification of miR6220, miR5070 and miR2118 and this value was optimized to 2 mM and 3 mM for the miR5085 and miR169 amplicons. [score:1]
The annealing temperature was optimized to 56°C for mi6220 and miR2118 quantification. [score:1]
Our experimental results supported our in silico predictions: 5DS was verified to harbour regions coding for pre-miR2118 and pre-miR5070, and 5DL was confirmeded to contain both of the above plus pre-miR6220, pre-miR5085 and miR169 coding regions. [score:1]
miR2118 was shown to be located on both arms of the 5D chromosome (5D specific), while miR5085 and miR169 were found to be specific to the long arm (Figure 2). [score:1]
For amplification of miR2118 and miR5070, the annealing temperature was optimized to 50 [o]C and 60,5 [o]C, respectively. [score:1]
In this study, five pre-miRNA (miR169, miR5085, miR2118, miR6220, miR2118) coding sequences were verified to be located to the 5D chromosome (Figure 2). [score:1]
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[+] score: 12
It is notable that Bdi-miR5163b-3p does not share sequence similarity with miR472/482, miR2118, miR1507 and miR2109/miR5213, which are known to target NB domains of NB-LRR and trigger phasiRNA generation [21, 22, 24, 67]. [score:3]
Examples of miRNAs initiating 21-nucleotide phased loci are miR173 [19], miR390 [25] and miR2118 [30], whereas miR2275 [30] is the only miRNA currently known that initiates 24-nucleotide phased loci. [score:1]
Additionally, members of the miR444 family (Additional file 2: Figure S1) and miR2118 were added manually. [score:1]
Examples of alternative RNA folding in precursors of Bdi-miR9483 (a), Bdi-miR2118 (b) and Bdi-miR166 (c). [score:1]
Bdi-miR2118 cleavage induces phasing. [score:1]
An example of the PARE and small RNA sequence distribution from representative loci initiated by miR2118 is shown in Additional file 2: Figure S4. [score:1]
The PARE sequences within five phase periods of these loci were pulled from the BDI25 PARE library and scored for complementarity to Bdi-miR390, Bdi-miR2118, Bdi-2275 and the 22-nucleotide small RNAs listed in Additional file 1: Table S2. [score:1]
Since Brachypodium produces miR390, miR2118 and miR2275, all known for initiating phased loci, these miRNAs may initiate some or all of these previously reported loci. [score:1]
Only about half of the phased loci were accounted for by miR390, miR2118 and miR2275. [score:1]
In rice and Brachypodium, 22-nucleotide miR2118 and miR2275 can trigger 21-nucleotide and 24-nucleotide phasiRNA generation, respectively, from hundreds of non-coding loci of unknown function [30- 32]. [score:1]
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[+] score: 4
In particular, Zhai et al. [83] highlighted the synthesis of miR2118 dependent 21 nt and miR2275 dependent 24 nt phasiRNAs, putatively implicated as mobile signals in anther development, coordinating cell-type specific expression [84]. [score:4]
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