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miRBase |
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![]() 9 publications mentioning hsa-mir-941-5Open access articles that are associated with the species Homo sapiens and mention the gene name mir-941-5. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary. |
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Other miRNAs from this paper: hsa-mir-21, hsa-mir-24-1, hsa-mir-34a, hsa-mir-27b, hsa-mir-137, hsa-mir-9-1, hsa-mir-9-3, hsa-mir-129-2, hsa-mir-193a, hsa-mir-34b, hsa-mir-375, hsa-mir-602, hsa-mir-1224, hsa-mir-941-1, hsa-mir-941-2, hsa-mir-941-3, hsa-mir-941-4, hsa-mir-1234, hsa-mir-1237, hsa-mir-663b, hsa-mir-1247
We postulated that down-regulation of these predicted targets by ectopic expression of miR-941 and miR-1247 would negatively impact cell migration.
[score:8]
We found that over -expression of miR-941 was able to significantly inhibit cell migration in both HCT116 and D KO cells and additionally in DLD1 cells.
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For instance, matrix metallopeptidase 24 (MMP24) is a predicted target for miR-941, and it facilitates tissue remo deling and cell migration in endometrium from endometriosis patients [36].
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Finally, similar suppressive effects on cell migration were independently observed in DLD1 cells (Figure S4C) and D KO cells (Figure S5C) transfected with miR-941 and miR-1247 mimics.
[score:3]
This result is reasonable considering several high priority predicted targets of miR-941 are related to cell migration.
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Over -expression of miR-941, miR-1237, and miR-1247 in HCT116, D KO, and DLD1 cells was achieved by transfecting the cells with 20 nM miRNA duplexes that mimicked the mature miR-941 (MSY0004984, Qiagen), miR-1237 (MSY0005592, Qiagen), and miR-1247 (MSY0005899, Qiagen).
[score:3]
Hence, we tested the effects of ectopic expression of miR-941, miR-1237, and miR-1247 on cell mobility in HCT116 cells.
[score:3]
To determine if expression of these epigenetically silenced miRNAs would affect cancer cell growth and migration, we transfected HCT116 cells with miRNA mimics of miR-941 (the mature form of miR-941-1 and -3), miR-1237, miR-1247, and a non-coding negative control (AllStars Neg.
[score:3]
Furthermore, computationally predicted targets of miR-941 and miR-1247 included several genes involved in cell migration and invasion.
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miR-941 and miR-1247 suppress cell growth and migration in colon cancer cells.
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We focused on two important aspects of cancer development, growth and migration, and selected miR-941, miR-1237, and miR-1247 for functional studies.
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Functional analysis of miR-941, miR-1237, and miR-1247 in HCT116 cells.
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), miR-941, or miR-1247 mimics.
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HCT116 cells transfected with miR-941 and miR-1247 mimics and the AllStars Negative Control were re-plated on coverslips at 24 hr post-transfection.
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), miR-941, miR-1237, or miR-1247 mimics.
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was carried out for (A) miR-941, (B) miR-1237, and (C) miR-1247 in parental HCT116 cells, HCT116 cells treated with 5 µM 5-aza-dC for 48 hr, and D KO cells.
[score:1]
), miR-941, or miR-1247.
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Taken together, these observations suggest that the epigenetic silencing of miR-941 and miR-1247 may facilitate colon cancer cell migration and invasion.
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0020628.g002 Figure 2Bisulfite sequencing was carried out for (A) miR-941, (B) miR-1237, and (C) miR-1247 in parental HCT116 cells, HCT116 cells treated with 5 µM 5-aza-dC for 48 hr, and D KO cells.
[score:1]
Figure S4 Functional analysis of miR-941, miR-1237, and miR-1247 in DLD1 cells.
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Our functional study of miR-941 substantiated this hypothesis.
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Figure S5 Functional analysis of miR-941 and miR-1247 in D KO cells.
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Other miRNAs from this paper: hsa-mir-29a, hsa-mir-140, hsa-mir-126, hsa-mir-26a-2, hsa-mir-374a, hsa-mir-589, hsa-mir-147b, hsa-mir-941-1, hsa-mir-941-2, hsa-mir-941-3, hsa-mir-941-4
Conversely, expression of MIR941 (50%, p < 0.05) and MIR589-5P (69%, p < 0.05), which were predicted by RNA-Seq analysis to be down regulated, were not validated by qPCR.
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We used qPCR to confirm expression patterns predicted by RNA-Seq analysis for 8 miRNA (MIR29A-5P, MIR140-5P, MIR126P, MIR374A-3P, MIR26A-2-3P and MIR147B, MIR941 and MIR589-5P) that were induced following treatment of any eCig liquid (ANY LIQUID).
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Also, mir-941 targets genes shown to be involved in insulin pathways and neurotransmitter signaling [80].
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Positive selection signature in miR-941 of Asian populations.
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Recently, it has been reportedthat mir-941 emerged de novo in humans from an evolutionarily volatile tandem repeat sequence, and multiple copies of this miRNA are present in the human genome [80].
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Although we have not observed continental specific differences for variants rs7360929 of mir-941-3, we did observe that, after scanning100 kb of the flanking region,the miR-941 region showed selection signatures in the Asian populations (shown in red color) while the flanking regions did not.
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Other miRNAs from this paper: hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-224, hsa-mir-299, hsa-mir-323a, hsa-mir-133b, hsa-mir-485, hsa-mir-497, hsa-mir-525, hsa-mir-507, hsa-mir-455, hsa-mir-539, hsa-mir-92b, hsa-mir-582, hsa-mir-548a-1, hsa-mir-548b, hsa-mir-548a-2, hsa-mir-590, hsa-mir-548a-3, hsa-mir-603, hsa-mir-548c, hsa-mir-622, hsa-mir-548d-1, hsa-mir-548d-2, hsa-mir-449b, hsa-mir-654, hsa-mir-920, hsa-mir-940, hsa-mir-941-1, hsa-mir-941-2, hsa-mir-941-3, hsa-mir-941-4, hsa-mir-548e, hsa-mir-548j, hsa-mir-548k, hsa-mir-548l, hsa-mir-548f-1, hsa-mir-548f-2, hsa-mir-548f-3, hsa-mir-548f-4, hsa-mir-548f-5, hsa-mir-548g, hsa-mir-548n, hsa-mir-548m, hsa-mir-548o, hsa-mir-548h-1, hsa-mir-548h-2, hsa-mir-548h-3, hsa-mir-548h-4, hsa-mir-548p, hsa-mir-548i-1, hsa-mir-548i-2, hsa-mir-548i-3, hsa-mir-548i-4, hsa-mir-548q, hsa-mir-548s, hsa-mir-548t, hsa-mir-548u, hsa-mir-548v, hsa-mir-323b, hsa-mir-548w, hsa-mir-548x, hsa-mir-548y, hsa-mir-548z, hsa-mir-548aa-1, hsa-mir-548aa-2, hsa-mir-548o-2, hsa-mir-548h-5, hsa-mir-548ab, hsa-mir-548ac, hsa-mir-548ad, hsa-mir-548ae-1, hsa-mir-548ae-2, hsa-mir-548ag-1, hsa-mir-548ag-2, hsa-mir-548ah, hsa-mir-548ai, hsa-mir-548aj-1, hsa-mir-548aj-2, hsa-mir-548x-2, hsa-mir-548ak, hsa-mir-548al, hsa-mir-548am, hsa-mir-548an, hsa-mir-548ao, hsa-mir-548ap, hsa-mir-548aq, hsa-mir-548ar, hsa-mir-548as, hsa-mir-548at, hsa-mir-548au, hsa-mir-548av, hsa-mir-548aw, hsa-mir-548ax, hsa-mir-548ay, hsa-mir-548az, hsa-mir-548ba, hsa-mir-548bb, hsa-mir-548bc
For the efficient target VCAM1, miR-941 repressed it only in normal stage, thus it had contribution to the ‘regulation of immune response’ process only in CIN I stage.
[score:4]
Our finding suggests that based on miR-941’s differential regulation on VCAM1, the leukocyte-endothelial cell adhesion may be promoted in CIN I stage, thus accelerating inflammation in local tissue.
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Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-31, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-199a-1, hsa-mir-199a-2, hsa-let-7g, hsa-let-7i, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-155, hsa-mir-486-1, hsa-mir-941-1, hsa-mir-941-2, hsa-mir-941-3, hsa-mir-941-4, hsa-mir-486-2
The smallest Student t-test P-value for a miRNA for nonprogressed vs progressed data was α=0.0053 (miR-941) (see s and Methods).
[score:1]
For example, the score 11 − 0 − 2 = 9 is reached for miR-199a-3p 5′-ACAGUAGUCUGCACAU_UGGUUA-3' and miR-941 5'-CACCCGGCUGUGUGCACAUGUGC-3′ because they contain the common subsequence GU-UGCACAU-UG (Supplementary Materials and Methods).
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[44]Restricting attention to the 40 most robustly expressed miRNAs (that include 3 miRNAs from equation (1), namely, miR-941, miR-103a-3p, and miR-92a-3p), we contrasted miRNA–miRNA correlation networks over the 3 groups by randomly selecting 1000 times subsets of 25 samples from each group and calculating all 780 Pearson correlations of pairs (from 40 miRNAs represented by 25-dimensional vectors).
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Notably, miR-941 and miR-103a-3p were both included in the correlation networks for unaffected and nonprogressed subjects but absent from the network for progressed subjects.
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[44] Restricting attention to the 40 most robustly expressed miRNAs (that include 3 miRNAs from equation (1), namely, miR-941, miR-103a-3p, and miR-92a-3p), we contrasted miRNA–miRNA correlation networks over the 3 groups by randomly selecting 1000 times subsets of 25 samples from each group and calculating all 780 Pearson correlations of pairs (from 40 miRNAs represented by 25-dimensional vectors).
[score:1]
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This host gene is repressed by miR-941, as well as two direct interacting partners of the protein encoded by DNAJC5 (ref.
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Based on DNAJC5 and its partner's functions, miR-941 was suggested to participate in neurotransmitter signalling 60.
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A remarkable example is the human-specific miR-941 hosted by DNAJC5.
[score:1]
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Other miRNAs from this paper: hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-191, hsa-mir-451a, hsa-mir-486-1, hsa-mir-941-1, hsa-mir-941-2, hsa-mir-941-3, hsa-mir-941-4, hsa-mir-4454, hsa-mir-486-2, hsa-mir-7977, hsa-mir-8069-1, hsa-mir-8069-2
Here, several miRNAs such as hsa-miR-941 (not detected in any array experiment, not detected in RT-qPCR, average read count of ~1000 reads using Illumina HiSeq sequencing) had expression levels differing several orders of magnitude between the miRBase microarrays and using HiSeq sequencing.
[score:3]
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Other miRNAs from this paper: hsa-mir-7-1, hsa-mir-7-2, hsa-mir-7-3, hsa-mir-941-1, hsa-mir-941-2, hsa-mir-941-3, hsa-mir-941-4
One recently investigated human-specific non-coding RNA, miR-941, is expressed in the brain and regulates genes involved in neurotransmitter signaling (Hu et al., 2012).
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Evolution of the human-specific microRNA miR-941.
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Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-16-1, hsa-mir-21, hsa-mir-26a-1, hsa-mir-29a, hsa-mir-98, hsa-mir-101-1, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-16-2, hsa-mir-196a-1, hsa-mir-148a, hsa-mir-139, hsa-mir-10b, hsa-mir-34a, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-196a-2, hsa-mir-203a, hsa-mir-205, hsa-mir-212, hsa-mir-214, hsa-mir-224, hsa-mir-200b, hsa-let-7g, hsa-let-7i, hsa-mir-15b, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-125b-1, hsa-mir-137, hsa-mir-141, hsa-mir-143, hsa-mir-144, hsa-mir-152, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125b-2, hsa-mir-127, hsa-mir-129-2, hsa-mir-185, hsa-mir-200c, hsa-mir-155, hsa-mir-181b-2, hsa-mir-29c, hsa-mir-200a, hsa-mir-101-2, hsa-mir-34b, hsa-mir-34c, hsa-mir-26a-2, hsa-mir-342, hsa-mir-196b, hsa-mir-429, hsa-mir-449a, hsa-mir-494, hsa-mir-519d, hsa-mir-449b, hsa-mir-449c, hsa-mir-708, hsa-mir-941-1, hsa-mir-941-2, hsa-mir-941-3, hsa-mir-941-4, hsa-mir-1237, hsa-mir-1247, hsa-mir-203b
In addition, Yan et al. (2011) performed a genome-wide methylome analysis entailing deep sequencing of MBD (methylated DNA binding domain)-isolated DNA in HCT116 cells, and identified a number of methylated genes, including miR-941, miR-1237, and miR-1247.
[score:1]
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