1 publications mentioning ata-MIR167a

Open access articles that are associated with the species Aegilops tauschii and mention the gene name MIR167a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

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Upon drought, however, miR167 was downregulated in T. aestivum (fourfold) but upregulated in A. tauschii (26 fold), likely resulting in a significant difference in miR167 accumulation in these two species.

Upon drought, miR167 was downregulated in T. aestivum, however, its expression was remarkably stimulated in A. tauschii (Figure 5).

The expressions of miR167, miR5175, and miR5205 were observed in both species, while miR5523 was expressed only in A. tauschii.

Four miRNAs (miR167, miR5175, miR5205, miR5523) were selected for quantification of expression in response to drought stress, the most prevalent stress condition causing severe yield losses worldwide.

FIGURE 5Expression levels of miR167, miR5175, and miR5205 in A. tauschii and T. aestivum seedlings in response to drought stress.

Under normal conditions, miR167 expression was 18 fold higher in T. aestivum than A. tauschii.

Under control conditions, miR167 expression appeared to be relatively high in T. aestivum, similar to previous observations that syntetic hexaploids (T. turgidum durum × A. tauschii) had higher miR167 levels, compared to the diploids (Kenan-Eichler et al., 2011).

In order to experimentally verify selected miRNAs, miR167, miR5175, miR5205 and miR5523, and quantify their expression levels in response to 4 h shock drought stress qRT-PCR using FastStart Universal SYBR Green Master (ROX; Mannheim, Germany) was performed with the following reaction mixture: 20 μl reaction included 3 μl RT stem–loop cDNA products, 10 μl 2× Master mix, 0.6 μl primers (300 nM each) and 6.4 μl nuclease-free water.

The annealing temperatures were optimized to 56°C for miR5523 and 58°C for miR167, miR5175, and miR5205.

Of the four selected miRNAs, miR167 is conserved among plants, including A. tauschii and wheat, and has been implicated in abiotic and biotic stress responses (Yao et al., 2007; Wei et al., 2009; Xin et al., 2010; Kenan-Eichler et al., 2011; Gupta et al., 2012; Tang et al., 2012; Jia et al., 2013; Li et al., 2013; Khaksefidi et al., 2015).

Two miRNA families, miR167 and miR2118 appeared to gain repetitive stem–loops in bread wheat.

In contrast, miR167, with the highest number of non-repetitive hairpins, did not have any repetitive hairpins and constituted only 1.60% of the overall representation.