![]() |
miRBase |
![]() |
![]() |
![]() 1 publications mentioning ata-MIR167aOpen access articles that are associated with the species Aegilops tauschii and mention the gene name MIR167a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary. |
|
1 |
![]()
Other miRNAs from this paper: tae-MIR167a, tae-MIR1117, tae-MIR1118, tae-MIR1120a, tae-MIR1121, tae-MIR1123, tae-MIR1125, tae-MIR1127a, tae-MIR1128, tae-MIR1130a, tae-MIR1131, tae-MIR1133, tae-MIR1135, tae-MIR1136, tae-MIR1137a, tae-MIR1139, tae-MIR167b, tae-MIR5086, tae-MIR1127b, tae-MIR1137b, tae-MIR167c, tae-MIR5175, tae-MIR1120b, tae-MIR1130b, tae-MIR1120c, tae-MIR6197, tae-MIR5049, ata-MIR167c, ata-MIR167e, ata-MIR2118c, ata-MIR5181, ata-MIR167b, ata-MIR2118a, ata-MIR167f, ata-MIR2118b, ata-MIR167d, ata-MIR2118d
Upon drought, however, miR167 was downregulated in T. aestivum (fourfold) but upregulated in A. tauschii (26 fold), likely resulting in a significant difference in miR167 accumulation in these two species.
[score:7]
Upon drought, miR167 was downregulated in T. aestivum, however, its expression was remarkably stimulated in A. tauschii (Figure 5).
[score:6]
The expressions of miR167, miR5175, and miR5205 were observed in both species, while miR5523 was expressed only in A. tauschii.
[score:5]
Four miRNAs (miR167, miR5175, miR5205, miR5523) were selected for quantification of expression in response to drought stress, the most prevalent stress condition causing severe yield losses worldwide.
[score:3]
FIGURE 5Expression levels of miR167, miR5175, and miR5205 in A. tauschii and T. aestivum seedlings in response to drought stress.
[score:3]
Under normal conditions, miR167 expression was 18 fold higher in T. aestivum than A. tauschii.
[score:3]
Under control conditions, miR167 expression appeared to be relatively high in T. aestivum, similar to previous observations that syntetic hexaploids (T. turgidum durum × A. tauschii) had higher miR167 levels, compared to the diploids (Kenan-Eichler et al., 2011).
[score:2]
In order to experimentally verify selected miRNAs, miR167, miR5175, miR5205 and miR5523, and quantify their expression levels in response to 4 h shock drought stress qRT-PCR using FastStart Universal SYBR Green Master (ROX; Mannheim, Germany) was performed with the following reaction mixture: 20 μl reaction included 3 μl RT stem–loop cDNA products, 10 μl 2× Master mix, 0.6 μl primers (300 nM each) and 6.4 μl nuclease-free water.
[score:2]
The annealing temperatures were optimized to 56°C for miR5523 and 58°C for miR167, miR5175, and miR5205.
[score:1]
Of the four selected miRNAs, miR167 is conserved among plants, including A. tauschii and wheat, and has been implicated in abiotic and biotic stress responses (Yao et al., 2007; Wei et al., 2009; Xin et al., 2010; Kenan-Eichler et al., 2011; Gupta et al., 2012; Tang et al., 2012; Jia et al., 2013; Li et al., 2013; Khaksefidi et al., 2015).
[score:1]
Two miRNA families, miR167 and miR2118 appeared to gain repetitive stem–loops in bread wheat.
[score:1]
In contrast, miR167, with the highest number of non-repetitive hairpins, did not have any repetitive hairpins and constituted only 1.60% of the overall representation.
[score:1]
[1 to 20 of 12 sentences]
|