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1 publications mentioning ata-MIR167a

Open access articles that are associated with the species Aegilops tauschii and mention the gene name MIR167a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

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[+] score: 35
Upon drought, however, miR167 was downregulated in T. aestivum (fourfold) but upregulated in A. tauschii (26 fold), likely resulting in a significant difference in miR167 accumulation in these two species. [score:7]
Upon drought, miR167 was downregulated in T. aestivum, however, its expression was remarkably stimulated in A. tauschii (Figure 5). [score:6]
The expressions of miR167, miR5175, and miR5205 were observed in both species, while miR5523 was expressed only in A. tauschii. [score:5]
Four miRNAs (miR167, miR5175, miR5205, miR5523) were selected for quantification of expression in response to drought stress, the most prevalent stress condition causing severe yield losses worldwide. [score:3]
FIGURE 5Expression levels of miR167, miR5175, and miR5205 in A. tauschii and T. aestivum seedlings in response to drought stress. [score:3]
Under normal conditions, miR167 expression was 18 fold higher in T. aestivum than A. tauschii. [score:3]
Under control conditions, miR167 expression appeared to be relatively high in T. aestivum, similar to previous observations that syntetic hexaploids (T. turgidum durum × A. tauschii) had higher miR167 levels, compared to the diploids (Kenan-Eichler et al., 2011). [score:2]
In order to experimentally verify selected miRNAs, miR167, miR5175, miR5205 and miR5523, and quantify their expression levels in response to 4 h shock drought stress qRT-PCR using FastStart Universal SYBR Green Master (ROX; Mannheim, Germany) was performed with the following reaction mixture: 20 μl reaction included 3 μl RT stem–loop cDNA products, 10 μl 2× Master mix, 0.6 μl primers (300 nM each) and 6.4 μl nuclease-free water. [score:2]
The annealing temperatures were optimized to 56°C for miR5523 and 58°C for miR167, miR5175, and miR5205. [score:1]
Of the four selected miRNAs, miR167 is conserved among plants, including A. tauschii and wheat, and has been implicated in abiotic and biotic stress responses (Yao et al., 2007; Wei et al., 2009; Xin et al., 2010; Kenan-Eichler et al., 2011; Gupta et al., 2012; Tang et al., 2012; Jia et al., 2013; Li et al., 2013; Khaksefidi et al., 2015). [score:1]
Two miRNA families, miR167 and miR2118 appeared to gain repetitive stem–loops in bread wheat. [score:1]
In contrast, miR167, with the highest number of non-repetitive hairpins, did not have any repetitive hairpins and constituted only 1.60% of the overall representation. [score:1]
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