sort by

7 publications mentioning mmu-mir-1271

Open access articles that are associated with the species Mus musculus and mention the gene name mir-1271. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

[+] score: 60
MicroRNAs miR-1271 FOXO1 Hepatocyte The expression of certain miRNAs targeting the molecules transducing insulin signaling is regulated aberrantly in saturated fatty acids (SFA) -induced obesity, and linked intimately to the pathogenesis of insulin resistance [2], [3]. [score:6]
Luciferase reporter constructs containing either a tentative miR-1271 target sequence in FOXO1 3′UTR (wild-type; FOXO1 3U wt), or three nucleotides mutant of the tentative target sequence (FOXO1 3U mut) were generated in the pmirGLO vector (Fig. 1B), as described in the Section 2. These reporter constructs, which included a Firefly luciferase cassette to allow normalization of the internal Renilla luciferase activity, were transfected transiently together with a scRNA control or miR-1271 mimic. [score:5]
Moreover, the transfection of miR-1271 mimics decreased significantly the protein expression of FOXO1 in HepG2 cells, whereas the expression of the β-actin control was unaffected (Fig. 2A). [score:5]
Previously, a dysregulation of miR-1271 expression was reported to be linked causally to the development of hepatic insulin resistance [1]. [score:5]
Human FOXO1 3′UTR, containing either the wild-type miR-1271 binding sites (FOXO1 3U wt) or the mutated miR-1271 binding sites (FOXO1 3U mut), was subcloned to the pmirGLO Dual-luciferase miRNA target expression vector (pmirGLO, Promega) with SacI and XbaI. [score:5]
This data article assessed the targets of miR-1271 on the insulin signaling pathway using in silico analysis, such as TargetScan, Pictar, and miRWalk. [score:5]
This suggests that miR-1271 targets FOXO1 3′UTR directly via its binding site. [score:4]
Interestingly, the transfection of miR-1271 decreased significantly the mRNA level of PEPCK, a downstream target gene of FOXO1 (Fig. 2B). [score:3]
FOXO1 was found to be one such predicted target of miR-1271 that belongs to this pathway (Fig. 1A). [score:3]
Fig. 1 Fig. 2Effect of miR-1271 on the expression of FOXO1 and PEPCK. [score:3]
This data article also presents accompanying data collected from a dual-luciferase reporter gene assay and immunoblotting to determine if FOXO1 would be a validated target of miR-1271 in hepatocytes. [score:2]
Fig. 1Targeting sites of miR-1271 in the 3′UTR of FOXO1, and an analysis of its binding by reporter gene assay. [score:2]
Mutations in the tentative miR-1271 binding site in the FOXO1 3′UTR (FOXO1 3U mut) abrogated the repressive effect of miR-1271 (Fig. 1C). [score:2]
For the Dual-luciferase target validation assay, the HepG2 cells were co -transfected with scRNA or miR-1271 mimics and pmirGLO vector containing the reporter genes using Lipofectamine 2000 (Invitrogen). [score:2]
As shown in Fig. 1C, co-transfection with the miR-1271 mimic and reporter construct (FOXO1 3U wt) inhibited the luciferase activity compared to the scRNA control. [score:2]
Further analysis of the data and insights into the implications of miR-1271 on the pathogenesis of insulin resistance and type 2 diabetes are reported elsewhere [1]. [score:1]
HepG2 cells were transfected with scRNA (200 nM) or miR-1271 (200 nM). [score:1]
HepG2 cells were reverse -transfected with the scrambled control miRNA (scRNA) or miR-1271 mimics using G-fectin (Genolution, Seoul, Korea) according to the manufacturer's instructions. [score:1]
The mutated 3′UTR of FOXO1 gene lacking the miR-1271 binding sites was examined (mutant; FOXO1 3U mut). [score:1]
First, the direct binding of miR-1271 to FOXO1 3′UTR was determined using a Dual luciferase -based reporter assay. [score:1]
2.3 HepG2 cells were reverse -transfected with the scrambled control miRNA (scRNA) or miR-1271 mimics using G-fectin (Genolution, Seoul, Korea) according to the manufacturer's instructions. [score:1]
[1 to 20 of 21 sentences]
[+] score: 5
Inhibition of DIXDC1 by microRNA-1271 suppresses the proliferation and invasion of prostate cancer cells. [score:5]
[1 to 20 of 1 sentences]
[+] score: 3
Other miRNAs from this paper: mmu-mir-130a, mmu-mir-196a-1, mmu-mir-196a-2
Wang Y. Xu L. Jiang L. miR-1271 promotes non-small-cell lung cancer cell proliferation and invasion via targeting HOXA5 Biochem. [score:3]
[1 to 20 of 1 sentences]
[+] score: 3
Firstly, not all the pre-miRNA sequences are expressed in the order of the genome sequence due to the RNA editing [39, 40], such as mir-381,mir-1271. [score:3]
[1 to 20 of 1 sentences]
[+] score: 2
Using significant microarray genes with a p < 0.01, ToppCluster identified 7 different miRNA binding sites, with 9 different miRNAs listed: MIR-32, MIR-92, MIR-96, MIR-129-5p, MIR-140-3p, MIR-141, MIR-200A, MIR-218, and MIR-1271. [score:1]
For example, the conserved binding site UUGGCAC can be bound by MIR-96 and MIR-1271. [score:1]
[1 to 20 of 2 sentences]
[+] score: 1
Xiao et al. confirmed the reduction of NPTX2 in AD and suggested a mechanism whereby NPTX2 reduction is probably caused by increased miR-1271 [19]. [score:1]
[1 to 20 of 1 sentences]
[+] score: 1
Other miRNAs from this paper: hsa-mir-25, hsa-mir-28, hsa-mir-95, mmu-mir-151, mmu-mir-290a, mmu-mir-297a-1, mmu-mir-297a-2, mmu-mir-130b, mmu-mir-340, mmu-mir-25, mmu-mir-28a, hsa-mir-130b, hsa-mir-367, hsa-mir-372, hsa-mir-378a, mmu-mir-378a, hsa-mir-340, hsa-mir-151a, mmu-mir-466a, mmu-mir-467a-1, hsa-mir-505, hsa-mir-506, mmu-mir-367, hsa-mir-92b, hsa-mir-548a-1, hsa-mir-548b, hsa-mir-548a-2, hsa-mir-548a-3, hsa-mir-548c, hsa-mir-648, hsa-mir-548d-1, hsa-mir-548d-2, hsa-mir-659, hsa-mir-421, hsa-mir-151b, hsa-mir-1271, hsa-mir-378d-2, mmu-mir-467b, mmu-mir-297b, mmu-mir-505, mmu-mir-297a-3, mmu-mir-297a-4, mmu-mir-297c, mmu-mir-421, mmu-mir-466b-1, mmu-mir-466b-2, mmu-mir-466b-3, mmu-mir-466c-1, mmu-mir-466e, mmu-mir-466f-1, mmu-mir-466f-2, mmu-mir-466f-3, mmu-mir-466g, mmu-mir-466h, mmu-mir-467c, mmu-mir-467d, mmu-mir-92b, mmu-mir-466d, hsa-mir-297, mmu-mir-467e, mmu-mir-466l, mmu-mir-669g, mmu-mir-466i, mmu-mir-466f-4, mmu-mir-466k, mmu-mir-467f, mmu-mir-466j, mmu-mir-467g, mmu-mir-467h, mmu-mir-1195, hsa-mir-548e, hsa-mir-548j, hsa-mir-1285-1, hsa-mir-1285-2, hsa-mir-1289-1, hsa-mir-1289-2, hsa-mir-548k, hsa-mir-1299, hsa-mir-548l, hsa-mir-1302-1, hsa-mir-1302-2, hsa-mir-1302-3, hsa-mir-1302-4, hsa-mir-1302-5, hsa-mir-1302-6, hsa-mir-1302-7, hsa-mir-1302-8, hsa-mir-548f-1, hsa-mir-548f-2, hsa-mir-548f-3, hsa-mir-548f-4, hsa-mir-548f-5, hsa-mir-1255a, hsa-mir-548g, hsa-mir-548n, hsa-mir-548m, hsa-mir-548o, hsa-mir-1268a, hsa-mir-548h-1, hsa-mir-548h-2, hsa-mir-548h-3, hsa-mir-548h-4, hsa-mir-548p, hsa-mir-548i-1, hsa-mir-548i-2, hsa-mir-548i-3, hsa-mir-548i-4, hsa-mir-1255b-1, hsa-mir-1255b-2, mmu-mir-1906-1, hsa-mir-1972-1, hsa-mir-548q, mmu-mir-466m, mmu-mir-466o, mmu-mir-467a-2, mmu-mir-467a-3, mmu-mir-466c-2, mmu-mir-467a-4, mmu-mir-466b-4, mmu-mir-467a-5, mmu-mir-466b-5, mmu-mir-467a-6, mmu-mir-466b-6, mmu-mir-467a-7, mmu-mir-466b-7, mmu-mir-467a-8, mmu-mir-467a-9, mmu-mir-467a-10, mmu-mir-466p, mmu-mir-466n, mmu-mir-466b-8, hsa-mir-3116-1, hsa-mir-3116-2, hsa-mir-3118-1, hsa-mir-3118-2, hsa-mir-3118-3, hsa-mir-548s, hsa-mir-378b, hsa-mir-466, hsa-mir-548t, hsa-mir-548u, hsa-mir-548v, hsa-mir-3156-1, hsa-mir-3118-4, hsa-mir-3174, hsa-mir-3179-1, hsa-mir-3179-2, hsa-mir-3179-3, hsa-mir-548w, hsa-mir-3156-2, hsa-mir-3156-3, hsa-mir-548x, mmu-mir-3470a, mmu-mir-3470b, mmu-mir-3471-1, mmu-mir-3471-2, hsa-mir-378c, hsa-mir-1972-2, hsa-mir-1302-9, hsa-mir-1302-10, hsa-mir-1302-11, mmu-mir-1906-2, hsa-mir-3683, hsa-mir-3690-1, hsa-mir-548y, hsa-mir-548z, hsa-mir-548aa-1, hsa-mir-548aa-2, hsa-mir-548o-2, hsa-mir-1268b, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-548h-5, hsa-mir-548ab, hsa-mir-378f, hsa-mir-378g, hsa-mir-548ac, hsa-mir-548ad, hsa-mir-548ae-1, hsa-mir-548ae-2, hsa-mir-548ag-1, hsa-mir-548ag-2, hsa-mir-548ah, hsa-mir-378h, hsa-mir-548ai, hsa-mir-548aj-1, hsa-mir-548aj-2, hsa-mir-548x-2, hsa-mir-548ak, hsa-mir-548al, hsa-mir-378i, hsa-mir-548am, hsa-mir-548an, mmu-mir-28c, mmu-mir-378b, mmu-mir-28b, hsa-mir-548ao, hsa-mir-548ap, mmu-mir-466q, hsa-mir-548aq, hsa-mir-548ar, hsa-mir-548as, hsa-mir-548at, hsa-mir-548au, hsa-mir-548av, hsa-mir-548aw, hsa-mir-548ax, hsa-mir-378j, mmu-mir-378c, mmu-mir-378d, hsa-mir-548ay, hsa-mir-548az, hsa-mir-3690-2, mmu-mir-290b, hsa-mir-548ba, hsa-mir-548bb, hsa-mir-3179-4, mmu-mir-466c-3, hsa-mir-548bc
The functional networks of miR-92b (PRdmiR, mir-25 family, derived from GC rich tandem repeats), miR-28 (RdmiR, mir-28 family, derived from LINE), miR-151 (RdmiR, mir-28 family, derived from LINE), miR-421 (RdmiR, mir-95 family, derived from LINE), miR-1271 (RdmiR, mir-1271 family, derived from LINE), miR-340 (RdmiR, mir-340 family, derived from DNA transportable element) and miR-378 (RdmiR, mir-378 family, derived from SINE) have been reconstructed (Figure 8). [score:1]
[1 to 20 of 1 sentences]