miRBase entry: hsa-mir-129-2

Stem-loop hsa-mir-129-2

HGNC: MIR129-2
Homo sapiens hsa-mir-129-2 precursor miRNA
Gene family
MIPF0000073; mir-129

Caution, this is an AI generated summary based on literature. This may have errors. ?

MIR129-2 is a microRNA that has been studied in various types of cancer, including Diffuse Intrinsic Pontine Glioma (DIPG) [PMC4494928]. In order to validate the role of MIR129-2 in targeting NG2 in human DIPG, primary DIPG lines (SF8628) were used [PMC4494928]. The association between MIR129-2 methylation and other tumor suppressive microRNA methylation, such as MIR34A, MIR124-1, MIR203, and MIR196B, was examined in patients with Multiple Myeloma (MM), Non-Hodgkin Lymphoma (NHL), and Chronic Lymphocytic Leukemia (CLL) using a Χ2 test [PMC3576298]. It was found that a CpG island is present near the promoter of MIR129-2 but not MIR129-1 [PMC3576298]. Furthermore, the frequency of MIR129-2 methylation was higher in MM patients at diagnosis compared to patients with Monoclonal Gammopathy of Undetermined Significance (MGUS) [PMC3576298]. The difference in frequency between MM and MGUS was statistically significant with a p-value of 0.023 [PMC3576298].

In summary, the role of MIR129-2 in targeting NG2 in human DIPG was validated using primary DIPG lines. Additionally, the association between methylation of MIR129-2 and other tumor suppressive microRNAs was examined in MM, NHL, and CLL patients. The presence of a CpG island near the promoter of MIR129-2 but not MIR129-1 suggests differential regulation between these two microRNAs. Furthermore, higher frequency of methylation at the CpG island was observed in MM patients at diagnosis compared to patients with MGUS.

Literature search
136 open access papers mention hsa-mir-129-2
(1245 sentences)


66020 reads, 302 reads per million, 95 experiments

u             -       C   CU      ---     acau 
 gcccuucgcgaau CUUUUUG GGU  GGGCUU   GCugu    a
 ||||||||||||| ||||||| |||  ||||||   |||||     
 cgggaggcguuUA GAAAAAC CCA  CCCGAA   cgaua    a
g             C       C   UU      ggc     acuc 

Annotation confidence High
Do you think this miRNA is real?
This miRNA sequence was predicted based on homology to a verified miRNA cloned from mouse cerebellum [1]. Expression of this miRNA was subsequently verified in a human osteoblast sarcoma cell line [2]. Reference [2] named the human/mouse conserved sequence miR-129b, but subsequent genome searches suggest that the same mature sequence may be expressed from two predicted hairpin precursors in both human (this entry and MIR:MI0000252) and mouse (MIR:MI0000222 and MIR:MI0000585). Landgraf et al. show that the 5' product of mir-129-1 (MIR:MI0000222) is the predominant one, whereas both 5' and 3' products are significantly expressed from mir-129-2 (this entry) [3].

Genome context
chr11: 43581394-43581483 [+]

Disease association
hsa-mir-129-2 is associated with one or more human diseases in the Human microRNA Disease Database
Disease Description Category PubMed ID

Database links

Mature hsa-miR-129-5p

Accession MIMAT0000242
Description Homo sapiens hsa-miR-129-5p mature miRNA
Evidence experimental
cloned [2-3]
Database links
Predicted targets

Mature hsa-miR-129-2-3p

Accession MIMAT0004605
Description Homo sapiens hsa-miR-129-2-3p mature miRNA
Evidence experimental
cloned [3]
Database links
Predicted targets


  1. PubMed ID: 17604727
    A mammalian microRNA expression atlas based on small RNA library sequencing
    "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
    "Cell (2007) 129:1401-1414

  2. PubMed ID: 12554859
    New microRNAs from mouse and human
    "Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T"
    "RNA (2003) 9:175-179

  3. PubMed ID: 12007417
    Identification of tissue-specific microRNAs from mouse
    "Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T"
    "Curr Biol (2002) 12:735-739